Articles in Press, Accepted Manuscript, Available Online from 20 February 2015
Abstract
Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called molecular ...
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Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called molecular signature is unique to a particular class of tumor or tumor cell. This paper describes the latest technique for monitoring the expression of a panel of cancer-specific genes. The PCR technique combines the quantitative performance of SYBR® Green-based real-time PCR is widely used for gene profiling. This technique is cost-effective, easy-to-use, and focuses only on the genes that you desire. In this study the expression of our target genes were quantitatively determined in five human cancer cell lines. We selected gene β-actin as our reference gene. Cells were lysed and the mRNAs were extracted using the RNA Purification Kit and cleaned up with Qiagen RNeasy spin columns. The first-strand cDNA was synthesized according to the High Capacity cDNA Reverse Transcription Kit protocol. RT-PCR were performed with Gene Expression Assays in an AB step one plus Sequence Detection System. Briefly the expression of p53 was high in both breast cancer cell lines, MCF7, T47-D and lung cancer cells, A549. Src expression was higher in prostate cell line, PC3 and lung cancer cells, A549. Meanwhile SKOV3 (ovarian cancer cell line) showed high expression of her-2 gene. The results clearly show that the expression pattern of this panel of genes was unique to almost every cell line examined.
Animal physiology
Articles in Press, Accepted Manuscript, Available Online from 20 November 2013
Abstract
Background: Obesity is often associated with micronutrient deficiency, the result of poor diet, lack of antioxidants and polyunsaturated fat on one side and saturated fats, on the other side. Oxidative stress has a role in the onset of chronic diseases associated with obesity.Plant-derived secondary ...
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Background: Obesity is often associated with micronutrient deficiency, the result of poor diet, lack of antioxidants and polyunsaturated fat on one side and saturated fats, on the other side. Oxidative stress has a role in the onset of chronic diseases associated with obesity.Plant-derived secondary metabolites such as phenolics and total flavonoids may have potential for free radical scavenging. The purpose of this study was to determine the effect of natural compounds such as thymol, acarbose,thioflavin T and Chalcone on serum total antioxidant levels in mice obese.Methods: This study was performed in NMRI male mice. Animals were randomly divided into two groups: a control group (receiving standard rodent diet) and experimental group for 8 weeks (receiving high-calorie diet group).The obese animals into three groups: sham,obese and experimental groups. In the experimental group of mice with compounds thymol, acarbose, thioflavin T and Chalcone(12mg/kg/day) were gavage for 8 weeks.The end of treatment, blood samples, to investigate serum total antioxidant capacity (Total Antioxidant Capacity, TAC) was performed .Results: Antioxidant capacity decreased in the obese group, the sham TAC levels than obese and control increased. In all treatment groups, TAC levels increased, but this increase in the thioflavin and chalcone group compared with the other groups was not significant; but in acarbose group (P
Articles in Press, Accepted Manuscript, Available Online from 20 November 2013
Abstract
Steroidal sex hormones especially testosterone have a protective effect in neurodegenerative events such as Alzheimer Disease. The present study was designed to explore effect of testosterone and androgen receptors in memory impairment induced by intra- cerebroventricular (icv) injection of streptozotocin ...
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Steroidal sex hormones especially testosterone have a protective effect in neurodegenerative events such as Alzheimer Disease. The present study was designed to explore effect of testosterone and androgen receptors in memory impairment induced by intra- cerebroventricular (icv) injection of streptozotocin (STZ) as a model of sporadic AD. In this research animals were divided randomly into 11 equal groups. Experimental model of AD was induced by bilateral icv injection of STZ. STZ-induced memory impairment was assessed two weeks after the last dose of STZ by using a passive avoidance task. The interval between the placement of animals in the illuminated chamber and the entry into the dark chamber was measured as step-through latency (STL). Castration was performed by surgical removing of testis and behavioral study of memory impairment was done after 4 weeks. Results of this study showed that icv injection of STZ could induce marked (p<0.001) memory impairment. Memory was worsened in castrated rats (P<0.001) when compared with normal and sham-operated animals. Testosterone replacement therapy in 4 week castrated rats restored memory up to the level of control groups. Testosterone had not any significant effect on memory impairments of non-castrated rats. According to the obtained results it can be concluded that testosterone improves cognitive and memory impairment of AD.
Microbiology
Articles in Press, Accepted Manuscript, Available Online from 20 November 2013
Abstract
Background and aims: E.coli is known one of the most important bacteria as it causes some problems such as urinary tract and noncosomial. In this study, the antimicrobial susceptibility of E.coli in the urine culture in the west of Mazandaran province was determined. Materials and methods: For this ...
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Background and aims: E.coli is known one of the most important bacteria as it causes some problems such as urinary tract and noncosomial. In this study, the antimicrobial susceptibility of E.coli in the urine culture in the west of Mazandaran province was determined. Materials and methods: For this descriptive study, a total of 357 urine samples were collected over a 12 month period, from September 2010 to September 2011. Then all specimens included in this study were routinely cultured on the EMB and MacConkey plates. All suspected colonies were identified and confirmed by Gram staining, colony characteristics, motility and biochemical reactions by MIVP tests for each isolates. Antibiogram tests were done by disc diffusion method according NCCLS standards. Results: Out of 357 specimens, 302 samples were positive cultures for E.coli. According to our results of antimicrobial tests, the highest susceptibilities were to Ciprofloxacin, Gentamicin, Nitrofurantoin with 48.4, 43.3 and 37.9 , respectively. Furthermore, lower than 10% of E.coli isolates were susceptible for antibiotics such as Ceftazidime,Tetracycline, Ampicilin, Erythromycin, Amoxicillin and Cefotaxime . Also statistical analysis by SPSS , chi square test, showed that sensitivity to some antibiotics such as Ceftriaxon, Nalidixic acid and Ceftizoxime was significant (χ2 test, P≤5) between men and women. Conclusion: in our study, the highest sensitivity for E.coli isolates was to ciprofloxicin. In conclusion, the rate of sensitivity for most of antibiotics, which had been tested, was not considerable and it can be alarming in this part of Mazandarn.
Articles in Press, Accepted Manuscript, Available Online from 23 August 2014
Abstract
Abstract Glucocorticoids are observed to increase at anxiety and different stressful situations. This augmentation is driven by stimulation of the hypothalamic-pituitary-adrenal axis which can threaten physical and mental health in long-term. Thus, this study compared the effects of diazepam and currant ...
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Abstract Glucocorticoids are observed to increase at anxiety and different stressful situations. This augmentation is driven by stimulation of the hypothalamic-pituitary-adrenal axis which can threaten physical and mental health in long-term. Thus, this study compared the effects of diazepam and currant hydroalcoholic extract on serum corticosterone level in adult male rat. In this study, 48 adult male mice were used in 6 groups of 8 animals, including the control (no treatment), Sham (receiving water) and 4 experimental groups receiving doses of 100, 200, 400 mg/kg from currant hydroalcoholic extract and 1 mg/kg of diazepam by gavage. After15days, rats perceived stressinelevatedplusmaze, after which corticosteronelevel wasmeasured in the serum of the blood collected from theirheart. Resultswere statically analyzedusingANOVAandTukey'stest. The results of this study showed that the doses of 200 and 400 mg/kg from currant extract significantly increases (P≤0.05) and diazepam significantly decreases (P≤0.01) the corticosterone levels I serum.Diazepamreducedcorticosteronelevelsin serum by affectingthe benzodiazepinereceptors and facilitatinginhibitory effect of GABA while currantextract increased it probablyby stimulatingnoradrenergicpathways.
Articles in Press, Accepted Manuscript, Available Online from 21 November 2013
Abstract
Peste des petits ruminants is a systemic and highly contagious disease in goats and sheep. Causing agent (Peste des petits ruminants virus) is a Morbillivirus in Paramyxoviridae. The disease is enzootic in Iran and the west of Iran especially Kermanshah province has a lot of serious problems in this ...
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Peste des petits ruminants is a systemic and highly contagious disease in goats and sheep. Causing agent (Peste des petits ruminants virus) is a Morbillivirus in Paramyxoviridae. The disease is enzootic in Iran and the west of Iran especially Kermanshah province has a lot of serious problems in this case. Therefore, in this study domestic small livestock were investigated by both serological (Competitive ELISA) and molecular (RT-PCR) methods in Kermanshah. The aim was comparison of sensitivity of these methods in diagnosis of PPR in early stage especially before diarrhea onset. By RT-PCR assay, 23 from 30 samples turned out negative and 7 has become positive (23.33% positive). 2 samples from sera related to those 7 animals were negative in ELISA test and the other 5 sera were positive. So, overlapping percent of the assays is 71.42. Moreover, sensitivity of PCR is 6.70% more than cELISA. In spite of more costs of the PCR than ELISA, accuracy and speed of PCR is a specific advantage which suggests virus isolation and competitive ELISA can be substituted by PCR to control and prevention of the illness. Regarding to the results, it is suggested to do more investigations on the prevalence of the disease in Iran to gain clear information about prevalence and risk factors of the infection in different parts of the country.
Articles in Press, Accepted Manuscript, Available Online from 23 August 2014
Abstract
Abstract 144juvenileSobaity,Sparidentexhasta,afteracclimationtolaboratoryconditionsinseawater(40ppt)foroneweek,seawaterwasreplacedwithdifferentsalinities(5,20and60ppt)andseawater(40ppt)ascontrol.Samplingswereperformedat6and12hours,1,2,7and14daysafterthetimeofchanginginenvironmentalsalinities.Bloodsampleswerecollectedforplasmacortisol,glucoseandelectrolytesanalysis.Accordingtotheresults,plasmalevelsofcortisolshowedsignificantincreasesin5and60pptenvironmentinrelationtocontroland20pptgroups12hoursfromthebeginningoftheexperiment.Howeverin24hsamplesuptotheendoftheexperiment(14days)therewerenosignificantdifferencesamongtreatments.Plasmaglucoselevelsshowedasignificantincreaseonlyat12hsamplingtimeinrelationto6and24hoursinfishadaptedto5and60ppt.PlasmaCa++concentrationshadnosignificantchangesduringexperiment.PlasmaMg++amountsdecreasedsignificantlyin5and20pptgroupsinrelationwithcontrolat24hsamplingtime.Inconclusion,cortisolhasimportantroleinadaptationtoboth5and60pptatthebeginningoftheexposure,becauseofitssignificantincreaseatjust12hoursin5and60pptthanthecontrols,buttheroleofcortisolhasbeenreplacedwithotherchangesinosmoregulatoryrelatedtissuesandcellsaccordingtotheplasmacortisolamountsin5and60pptthatreturnedtothebasiclevels
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Abstract 144juvenileSobaity,Sparidentexhasta,afteracclimationtolaboratoryconditionsinseawater(40ppt)foroneweek,seawaterwasreplacedwithdifferentsalinities(5,20and60ppt)andseawater(40ppt)ascontrol.Samplingswereperformedat6and12hours,1,2,7and14daysafterthetimeofchanginginenvironmentalsalinities.Bloodsampleswerecollectedforplasmacortisol,glucoseandelectrolytesanalysis.Accordingtotheresults,plasmalevelsofcortisolshowedsignificantincreasesin5and60pptenvironmentinrelationtocontroland20pptgroups12hoursfromthebeginningoftheexperiment.Howeverin24hsamplesuptotheendoftheexperiment(14days)therewerenosignificantdifferencesamongtreatments.Plasmaglucoselevelsshowedasignificantincreaseonlyat12hsamplingtimeinrelationto6and24hoursinfishadaptedto5and60ppt.PlasmaCa++concentrationshadnosignificantchangesduringexperiment.PlasmaMg++amountsdecreasedsignificantlyin5and20pptgroupsinrelationwithcontrolat24hsamplingtime.Inconclusion,cortisolhasimportantroleinadaptationtoboth5and60pptatthebeginningoftheexposure,becauseofitssignificantincreaseatjust12hoursin5and60pptthanthecontrols,buttheroleofcortisolhasbeenreplacedwithotherchangesinosmoregulatoryrelatedtissuesandcellsaccordingtotheplasmacortisolamountsin5and60pptthatreturnedtothebasiclevels
Biophysics
Amir Sabet
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Nowadays the world is full of some less known species of signals which connect us together. So human beings are continually exposed to electric, magnetic and electromagnetic fields. In order to investigate the effects of magnetic fields on cell survival, bone marrow mesenchymal stem cells were exposed ...
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Nowadays the world is full of some less known species of signals which connect us together. So human beings are continually exposed to electric, magnetic and electromagnetic fields. In order to investigate the effects of magnetic fields on cell survival, bone marrow mesenchymal stem cells were exposed to 15 mT static magnetic field in the presence and the absence of 0.5 gray X-ray. The rate of dead cells didn't show a significant difference when the cells were treated with static magnetic field alone. But after an acute exposure with the ionizing radiation, the field increased the percentage of survived cells significantly and rescued a part of X-ray induced apoptotic cells. These data show that static magnetic field treatment, suppresses apoptosis and promotes survival in bone marrow stem cells of rat. Thus, magnetic fields might act as a co-mutagenic and co-carcinogenic agent with increasing the risk of tumor development by inhibiting apoptosis.
Articles in Press, Accepted Manuscript, Available Online from 22 May 2014
Abstract
Abstract Inthis survey anatomical and histological aspects of testis and seminiferous tubules during post-hibernation period in Laudakianupta inhabiting the north of Lorestan province in Zagros Mountains were investigated. To execute this task male lizard’s samples were collected from the ...
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Abstract Inthis survey anatomical and histological aspects of testis and seminiferous tubules during post-hibernation period in Laudakianupta inhabiting the north of Lorestan province in Zagros Mountains were investigated. To execute this task male lizard’s samples were collected from the study area during April to September for two consecutive years. In the laboratory gonads were removed and passed through fixation, dehydration, clearing, infiltration and embedding stages. By means of a rotary microtome serial sections were cut and stained by Haematoxylin-Eosin. Using light microscopy, quantitative and qualitative examinations were then performed on the sections. Our results revealed that during April to September the diameter of seminiferous tubules and the thickness of germinal epithelium constantly decrease. In addition, during the April and May the germinal cells are undergoing the meiosis or maturing process but no spermatozoid is found in the tubules yet. During June and July, the spermatogenesis process reaches to its maximum peak and mature spermatozoa are constantly released into the tubules lumen. During August to September, the production of sperm ceases and the tubules begin a reduction process. In general, the results displayed that the type of spermatogenesis in this taxon is classified as associated and spermatogenic activity peaks when there are high temperatures and long photoperiod which coincides with June and July.
Articles in Press, Accepted Manuscript, Available Online from 20 February 2015
Abstract
Abstract The present study was investigated artichoke extract in drinking water on performance, carcass characteristics and some blood parameters concentrations such as Triglyceride, Cholesterol, very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) in ...
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Abstract The present study was investigated artichoke extract in drinking water on performance, carcass characteristics and some blood parameters concentrations such as Triglyceride, Cholesterol, very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) in broiler chickens. Experiment was used by randomized complete block design (RCBD) with 200 broiler chickens consisted of 5 treatments 0, 100, 200, 300 and 500 milligrams artichoke extract per water liter at four replicates per treatment and 10 chickens per replication during 21-35 days of age. Artichoke extract had any significant effect on feed intake, body weight gain, feed conversion ration and carcass characteristics. Artichoke extract had any significant effect on the blood parameters in broiler chickens during 35 day of age. It can be concluded that inclusion extract of artichoke up to 500 milligrams per liter has no beneficial consequences on performance and blood serum fat constituents.
Articles in Press, Accepted Manuscript, Available Online from 22 May 2014
Abstract
Abstract Zoning and implementation of strict program for each zone must be done to procurement of management goals in protected areas. In order to evaluate of ecological potential of Kuh-e Gorm non- hunting area (83410 ha) in Fars province, possibility of change it to protected area and land management, ...
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Abstract Zoning and implementation of strict program for each zone must be done to procurement of management goals in protected areas. In order to evaluate of ecological potential of Kuh-e Gorm non- hunting area (83410 ha) in Fars province, possibility of change it to protected area and land management, zoning be performed using GIS software. Thus, to pursue the purposes, nocturnal and daily survey was done to identify vegetation communities and animal species. Ecological and socio-economic resources were initially surveyed and mapped (1:100.000). Overlaying of different maps creates final map of environmental units (with 625 environmental units). Table of environmental units was completed and positional data were analyzed, then area was zoned for above purposes. The result show that 7 zones are possible in the area;10.74% of the area is suitable for restricted nature reserve, 31.86% for protected area zone, 2.40% for restricted use zone, 46.88 for extensive use zone, 4.11 for rehabilitation zone, 0.61 for special use zone and 3.40 for buffer zone. In total 42% of allocated zones are suitable for conservational proposes. Some animal species such as Capra aegagrus, Lutra lutra, Dryomys nitedula, Aquila chrysaetos, Falco tinnunculus, varanus griseus and Testudo graeca are important in term of conservational value.
Biophysics
Mostafa Jamalan; Majid Zeinali; Ebrahim Asadabadi
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Overexpression and malfunction of epidermal growth factor receptors (EGFRs) is associated with occurrence of malignancy in various tissues. Among all members of EGFRs, HER2 as an orphan receptor dimerized and activated without presence of any kind of ligands has significant role in incidence of breast ...
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Overexpression and malfunction of epidermal growth factor receptors (EGFRs) is associated with occurrence of malignancy in various tissues. Among all members of EGFRs, HER2 as an orphan receptor dimerized and activated without presence of any kind of ligands has significant role in incidence of breast cancer. Herceptin as approved therapeutic monoclonal antibody targets extracellular domain of HER2 and inhibits its dimerization and prevents induction of intracellular signaling cascades. Efficiency of point mutation which offered based on rational design approach for affinity maturation of Herceptin was investigated by 10 ns of molecular dynamic simulation. According to our results, Herceptin with mutated light chain make a more stable complex with HER2 compared to its native type. Asp92 in mutated VL of Herceptin constructs a stable salt bridge with Lys569 on HER2, which decreases the electrostatic energy between VL of Herceptin and HER2, thereby affecting RMSD values of IV-HER2 during 10 ns of MD simulation. Herceptin (VL; Tyr92Asp)-HER2 complex has lower level of total energy during allover of MD simulation compared to Herceptin-HER2 complex. Although Herceptin has high Kd of 5 nM, it has great potential to be enhanced. Our molecular modeling investigation demonstrated that affinity maturation of Herceptin through rational design could be performed via mutation of Tyr92 to Asp in VL chain of antibody. Improvement of Herceptin affinity for HER2 could increase its efficiency and result to reduction of its dose or frequency of administrations.
Animal physiology
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
The association between ABO blood groups and Helicobacter pylori seroprevalence is still controversial. We aimed to study the association between ABO/Rh antigens and IgG seropositivity in an Iranian adult population for the first time. Blood samples of 300 healthy young adults aging 18 -30 years were ...
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The association between ABO blood groups and Helicobacter pylori seroprevalence is still controversial. We aimed to study the association between ABO/Rh antigens and IgG seropositivity in an Iranian adult population for the first time. Blood samples of 300 healthy young adults aging 18 -30 years were collected for H. pylori antibody detection and ABO/Rhesus blood group antigen typing. 51.2 %, 49.5 %, 52.5 % and 60.0 % of cases with blood groups O, A, B and AB were seropositive, respectively. The frequency of seropositivity was not statistically different among the four blood groups (P = 0.843). No association was found between seropositivity and either Rh antigen (P = 0.850) or gender (P = 0.377). Seropositive individuals were significantly older than seronegative ones (24.48 ± 3.72 years vs. 23.25 ± 3.88 years; P = 0.006). There was a sharp increase in the prevalence of seropositivity in the 21-24 year age group compared to the 18-21 year age group and in the 27-30 year age group compared to 24-27 year age group. Adults in Iran seem to be more prone to new H. pylori infection in the beginning and the end of their twenties than the years in between.
Development
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; ...
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Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; the released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometric analysis were done for CD105, CD90, CD146, CD31 and CD34 at third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated at the third passage after 21 days of induction.The flowcytometry analysis showed that EnSCs were positive for CD90, CD105, CD146 and were negative for CD31, CD34. Moreover we show that the hEnSCs can differentiate in osteocyte and adipocyte, as confirmed by alizarin Red and oil-Red O staining. These differentiated cells into neuron and endothelial express related markers including Nestin, MAP2 and CD31. Results show that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.
animal biosystematic
rose salatin
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
lepidoptera class consists of butterflies and moths.this class has about 165000 species all over the world which 20000of these species are butterflies.Rhopalocera subclass which belongs to lepidoptera class, is the most familiar and specified of insects.these insects have considerable economical importance ...
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lepidoptera class consists of butterflies and moths.this class has about 165000 species all over the world which 20000of these species are butterflies.Rhopalocera subclass which belongs to lepidoptera class, is the most familiar and specified of insects.these insects have considerable economical importance ,most of these species larvae are vegeterian and a large number of them are crop pests. on the other hand,in the mature stage ,they play an essential role in flowers pollination and growth.a lot of these mature species are gathered for their beauty by collectors and they are used as models in art and drawing. this study has done to complete Elborz butterflies fauna. in researches during spring and summer of 2010 on butterflies fauna in some parts of central Elborz(Dizin, Galandrud, Kamarbon, Nesen, Kodir and Baladeh), 170 butterfly samples of nymphalidae family were gathered by insect net and specified which among them 6 species belong to 3 genus and 2 tribes belong to nymphalinae subfamily.
zahra zamani
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
For environmental safety and reduce the effect of pesticide, for the first report we used the enthomopathogenic fungi Lecanicillium muscarium and Ferula assafoetida extract for Biological control of keys pest that have wide damages to our crops. We isolate the pathogenic strain KB512 of enthomopathogenic ...
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For environmental safety and reduce the effect of pesticide, for the first report we used the enthomopathogenic fungi Lecanicillium muscarium and Ferula assafoetida extract for Biological control of keys pest that have wide damages to our crops. We isolate the pathogenic strain KB512 of enthomopathogenic fungi Lecanicillium muscarium from the soil and recognized.the pathogencity test was carried out with direct insect spray. Bioassay with concentration،1×108 conidi / ml were tested and the control were spraied by 0/01% tween80 in distilled water after spraying the pest, plat were placed in the incubator with temperature of 25±1 and RH80%.Tested pest were monitoring every day for checking the growth of the fungi ,after 9,7,5 days the percentage of mortality in treatment with ( 1×108 conidi/ml) concentration respectively was 36%-25%-17/26% and Ferula assafoetida extract was 40/96%,27/02%,18/62%for Aphis faba, and treatment with ( 1×108 conidi/ml) concentration 40/66%-28/79%-24% % and treatment with Ferula assafoetida extract was 38/9%,35/68%,32/02% for Bemisia tabaci
Safieh Soufian
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Occult hepatitis B infection (OHB) is defined as infection with detectable HBV DNA and undetectable surface antigen (HBsAg) in patients' blood. It is believed that detection of hepatitis B virus by host in “a” region of surface antigen (HBsAg) is performed. However, there have never been successful ...
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Occult hepatitis B infection (OHB) is defined as infection with detectable HBV DNA and undetectable surface antigen (HBsAg) in patients' blood. It is believed that detection of hepatitis B virus by host in “a” region of surface antigen (HBsAg) is performed. However, there have never been successful attempts to provide evidence for this hypothesis, partly because the 3D structure of HBsAg molecule has not been determined. Tertiary structure prediction of HBsAg solely from its primary amino acid sequence may reveal the structural changes of the mutant proteins. We carried out this study to analyze the predicted HBsAg conformation changes of OBH variant and wild isolated from Iranian patient blood. We extracted HBsAg sequences (OBH and wilde) of Iranian from Gen Bank. Tertiary structures of the wild & occult HBsAg were predicted using “ab initio” modeling and I-TASSER method. Then visualized using deep View/Siwss-pdb Viewer. Dipole moments of 3D structures were determined by chimera software. Wild & occult HBsAg sequences were analyzed in CLC Main Workbench to prediction antigenic index of each sequence. Our results showed the single amino acid substitutions in OBH, may alter structural properties of variant HBsAg, which can be shown by both its antigenic index and dipole moment of predicted 3D conformation. This survey suggested that the moreover “a" region mutations, other mutations can be change structure of HBsAg. It suggested to detection and vaccine design should be pay attention to three-dimensional structure of HbsAg.
Fisheries
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
In this study acute toxicity (96h LC50) of EDTA has been carried out in laboratory conditions in 2011 fall for rainbow trout. The experiments were conucted for 96h with standard method (O.E.C.D). LC50 values of EDTA at 96 h were 2231 mgl-1, to the rainbow trout. Then, individuals with a body weight of ...
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In this study acute toxicity (96h LC50) of EDTA has been carried out in laboratory conditions in 2011 fall for rainbow trout. The experiments were conucted for 96h with standard method (O.E.C.D). LC50 values of EDTA at 96 h were 2231 mgl-1, to the rainbow trout. Then, individuals with a body weight of 51 ± 12 g and length 18.5 ± 2.1 cm were selected for six groups (five test group and control group). Onchorhychus mykiss were exposed to 800, 1100, 1400, 1700 and 2100 mgl-1 of EDTA. Fish were exposed for 96 h. The experiments periods were controlled water physicochemical factors such as: pH, total hardness, dissolved oxygen and temperature. For analysis of all data SPSS software program was used. Haematology results show that exposure to EDTA causes an increase in leucocyte count (WBC), notrophil, eosinophil, monocyte and a decrease in haemoglobin (Hb), haematocrit (HCT) and lymphocyte (P0.05). The results showed that exposure to low concentrations of EDTA causes changes in some haematological parameters of rainbow trout and may weaken the fish immune system.
Veterinary
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment ...
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The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment containing 5 pen and 15 birds on each pen. Diets included 0, 1 or 1.5gr of probiotic BIOSAF/kg diet and considered as control, treatment1 and treatment 2, respectively. On day 20 and 35 of age, birds were injected (i.m) with 0.1 mg of 0.5% sheep red blood cells (SRBC). Antibody production against SRBC was measured after 7 days of inoculation. On the day 10 of age, the chicks were vaccinated against Newcastle disease virus (NDV Generally, usingprobiotic BIOSAF per kg diet has efficient effect on growth performance of male Ross chekien particularly, when supplementation is done in starter period. Also, supplementation probiotic BIOSAF has positive effect on immune response to Newcastle and SRBC. However, 1gr/kg supplementation of probiotic BIOSAF is more suitable than 1.5gr/kg diet.
Fisheries
seyedeh abedi; mohammad khalesi; Sohrab Eskandari
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity ...
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This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity factor (TF) was determined for each species. For bioaccumulation trial, the two species exposed to sublethal concentrations (10% of LC50 values) of Cr at separate triplicate treatments for 15 days. The examined tissues from each species were sampled at days 7 and 15 of the experiment. Following chemical digestion, each tissue was analyzed by atomic absorption unit (Model Thermo) to measure chromium concentration. According to the results, Almost all differences in bioaccumulations between the two species and the controls were significant (p < 0.05). Concentration of chromium in common carp was in the form of gills> liver> skin> scales> muscle, and accumulation in catfish was in the form of liver> muscle> skin> gills. Final entire contents of Cr in the catfish's tissues were 3.65 times, than in the carp’s (excluding the scales). In addition, the catfish's muscle amassed 16.15 times greater amounts of Cr.The results of this investigation indicate that the carp's scaled armature, compared to the scaleless skin of the catfish, adsorbs metal toxicants leading to much lesser metals content in the muscle rendering this tissue rather safer for comestible consumption.
Veterinary
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity ...
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The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity (GPX units/ml PCV) and Total Antioxidant Status (TASmmol/L). Thirty six pregnant Holstein dairy cows were assigned into two treatment groups based on their BCS and expected.calving date in t student examination. Treatment groups consisted of 1)glutamine supplementation 100 g/d per cow from 21d before calving until parturition (F), 2) glutamine unsupplementation from 21d before calving until parturition (N). There weren’t any significant differences among treatments in DMI and BCS on d -21,-14 ,-7 and calving day before parturition. There weren’t significant differences in The total antioxidant status (TAS) but plasma glutathione Peroxidase activity (GPX) was significant difference between two group and was greater for (F) group at -7d before calving (57.44 vs 47.94 and p≤0.05 respectively) and -14 (45.87 vs 41.72 and p≤0.04 respectively) before parturition. It seems that supplementation diets with glutamine on the close up period can enhance plasma glutathione Peroxides activity (GPX) and the best level of formaldehyde for protection of glutamine is 1%.
animal biosystematic
iman gowhari; shahrokh pashaei rad
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Based on the published statistics of diseases management center, yearly frequency of scorpion bite in Iran between 2001 to 2003 has been 38415 bite and the rate of death wich is resultant of scorpion bite has been 21 cases during a year. Doubtless scorpion bite is one of our medical problems, but what ...
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Based on the published statistics of diseases management center, yearly frequency of scorpion bite in Iran between 2001 to 2003 has been 38415 bite and the rate of death wich is resultant of scorpion bite has been 21 cases during a year. Doubtless scorpion bite is one of our medical problems, but what is important regarding this matter is a fear that people have about this creature. Unfortunately a precise and right statistcs toward the risk of this creature bite does not exist. Till based on that, it woud be possible to recognize scorpion bite of our society is related to what species and it has what a status among category of death causes and based on that prepre a antivenom monovalan related to every scorpion؛ surely we can not deny this problem in south and west regions of our country specially for hot seasons.In this research first, Ilam province based on geography position, scorpion bitting domain, climate and local researches divided to 14 region of sampling. By use of valid identifying keys such as Navid pour(2008), samples recognizes, frequency of scorpion species in each climate region has showed and their distribution map by use of GIS software has drawn. In Ilam province, among 390 hunted scorpion, 10 species include Hottentotta saulcyi(23/07), Mesobuthus eupeus(22/56), Compsobuthus matthiesseni(13/07), hemiscorpius lepturus(12/3), Orthochirus iranus(7/94), Razianus zarudnyi(4/87), Androctonus crassicaudata(4/1), Buthacus macrocentrus(3/33), Scorpio maurus(2/56), Odontobuthus bidentatus(1/02) scorpion identified.
Animal physiology
Seyed Ebrahim Hosseini; Mozhde Heidari
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Introduction: Due to the increasing use of high intake of saturated fatty acids and their effects on overall physiological structures, effects of unsaturated fatty acids, including omega-3 and omega-6, and the proportion of these two together is essential physiological functions. Therefore, this study ...
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Introduction: Due to the increasing use of high intake of saturated fatty acids and their effects on overall physiological structures, effects of unsaturated fatty acids, including omega-3 and omega-6, and the proportion of these two together is essential physiological functions. Therefore, this study aimed to investigate the interaction of corn and salmon fish’s oil on avoidance learning in mature male rat.Method: In this empirical research work, we used 72 mature male rats weighing from 180-200g were enrolled as the control, sham and experiment groups. The control group was no treated. The sham group received only 1 ml saline, 6 experimental groups of different types received 0.5, 1 and 1.5 ml/kg body weight corn oil, and 0.25, 0.5 and 0.75 ml/kg body weight and 1 group other received doses of 0.5 and 1.5, salmon oil and corn oil respectively, daily for 28 days. For avoidance learning behavior, the rats were trained to shuttle box and 24 hours after training, the rats were tested retrieval. The data were evaluated using ANOVA.Results: The results showed that consumption of corn and salmon’s oil alone enhances learning and taking the time to arrange a 3 to 1 ratio, boost the oil alone is more than the avoidance learning.Discussion and conclusion: Omega-3 and Omega-6 fatty acids the rate 3 to 1, are most effective in promoting avoidance learning, so further investigation can be listed using ratios to enhance learning and prevention of Alzheimer's, it uses .
Animal physiology
mokhtar fathi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied ...
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A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group set as control (healthy) group and reared under normal temperature, second group set as PHS group and reared under cold temperature. At the end of the experiment (day42), 2 chicks from each replicate were randomly selected bled and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. The results showed that hematocrit value, mortality due to ascites, RV/TV index and differential white blood cells count were significantly higher in CT treatment chick (P˂0.05). The concentrations of plasma t4 of CT birds were significantly lower than other group birds at day 21 & 42. it is also, The concentrations of plasma t3 of CT birds were significantly higher than other group birds at day 21(exception day 42) (P˂0.05).
histology
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured ...
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To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured by method Enzymatic, serum levels of lipoproteins were determined by method Precipitation and serum levels of testosterone were measured by method Radio Immuno Assay. For histomorphometric study, the 5 µ sections were made and stained with Hematoxyline-Eosin. Data was statistically analyzed by the one way ANOVA and Pearson correlation tests (p≤0.05). Results showed that serum levels of total cholesterol, triglycerids, VLDL-c and LDL-c had not significant differences between different ages. The serum levels of HDL-c, testosterone, as well as the area of spermatic tubules and testicular interstitium, the diameter of seminiferous tubules, the germinal epithelium height, the number and diameters of the leydig cells; additionally, the number of spermatid cells had significant increase from one year group to two years group and also significant decrease from two years animals to three years group. The serum HDL-c values were significantly correlated with the number of leydig cells as well as serum levels of testosterone. Serum triglycerides values were also inversely correlated with germinal epithelium height. It can be concluded that increased serum levels of HDL-c and triglycerides have respectively a benefit and deleterious effects on the spermatogenic activity and histomorphometric structure of the testis in the male goats of Ilam province.
Fisheries
Elham Abdzadeh; Fatemeh Nazarhaghighi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, ...
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To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor,with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and blood plasma were measured inorganic phosphorous. The results showed that the cortisol and glucose levels on day 5, there was a significant difference between control and Butachlor treatments (P 0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P
Mehrdad Modaresi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
The scientific name of a dark mountain celery is Apium graveolens. This plant contains phytoestrogens and has many effects on the reproductive system. In this study, hydroalcoholic leaf extract on the testicular tissue and spermatogenesis in male mice was investigated. The samples were randomly divided ...
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The scientific name of a dark mountain celery is Apium graveolens. This plant contains phytoestrogens and has many effects on the reproductive system. In this study, hydroalcoholic leaf extract on the testicular tissue and spermatogenesis in male mice was investigated. The samples were randomly divided into 5 groups (three treatment groups , placebo and control) groups, and all samples were stored in the same condition. Apium leaves hydroalcoholic extract in different doses (mg/kg/2day150, 100,50) was injected (IP) into the treated groups for 20 days (one day in between) . The Placebo group were used for injection of saline. The parameters that were evaluated changes , testicular weight and histology of spermatogenesis was compared with control groups. Data were analyzed by SPSS software and statistical tests one way ANOVA and Duncan test.
This study showed dose-dependent negative effect of Apium extract on spermatogenesis and reproductive potential in male rats.
Keywords : Apium graveolens, Mice, spermatogenesis
Medical physiology
Sayeh Bidaran; Sima Nasri
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Introduction & Objective: Zinc is an essential element for the immune system. This element has different effects on immune system activity. On the other hand different serum Zn levels also alter normal B-cell line and T-cell line functions. The present study was carried out to assess B cell line and ...
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Introduction & Objective: Zinc is an essential element for the immune system. This element has different effects on immune system activity. On the other hand different serum Zn levels also alter normal B-cell line and T-cell line functions. The present study was carried out to assess B cell line and T cell line tolerance to low concentration Zinc Chloride In vitro studyMaterials & Methods: This experimental study was conducted at the Payame Noor University of Tehran in 2010. The B- cell line and T-cell line were exposed to 1, 5, 10, 20, 30, 40, 50, 75, 100,125, 150,175 and 200 µm (λ) of Zinc Chloride followed by incubation at 12, 24, 36, 48, 60 and 72 hrs. Viability of B- cell line (Raji) and T-cell line (molt-4) were evaluated with hemocytometer method and percentage of living cells have been considered as cellular tolerance index to cytotoxic effects of Zinc chloride. The results were analyzed by SPSS software, version 18 using T-test and Kruskal-Wallis testResults: In this study, the results showed Zinc chloride concentrations up to 40 µm (λ) at different incubation time points (12-72 hrs) had no effects on B cell line and T cell line viability( p> 0.05) With 50-200 µm (λ) concentrations, Zinc at different incubation time points(12-72 hrs)
animal biosystematic
Valiallah Khalaji-Pirbalouty
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the ...
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In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the detailed drawings are made using special pens and proper lines. The final illustrations produced with this method can be stored in all known formats and used in the scientific literatures. The digitizer panel, as an alternative option, may be used to schedule the initial drawing and also adding the details.Key words: Animal illustrations, CorelDraw, Digital drawings.In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the detailed drawings are made using special pens and proper lines. The final illustrations produced with this method can be stored in all known formats and used in the scientific literatures. The digitizer panel, as an alternative option, may be used to schedule the initial drawing and also adding the details.Key words: Animal illustrations, CorelDraw, Digital drawings
Veterinary
Navab Ghobadi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and ...
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The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and second Semenation, amount of pregnancy at first semenation and number of semenation for every pregnancy. In This study 30 Holeshtain dairy cow are used in 2 group of 15(treatment and control group).treatment group recivead 10cc Oxytocin immidiatly after parturition and 5cc prostsglandinF2α,20 to 25 days after parturition.the result statistically using chi sequare and t student test were analyzed with spss.The result show a meaning ful difference(p≥0/05)in uterus contraction ,amount of pregnancy at first Semenation and number of Semenation for every pregnancy between treatment and control group.there is a noticable improvement in percent of pregnancy for Treatment group(47/1percent for treatment group and 23/5percent for control group).The result also shows that injecting oxytocin immidiatly after parturition and prostaglandinf2α 20 to25 days later can hare positive increase in amount of future fertility in dairy cow and also it can decrease economical loss in cow herd. This result can show connection of contraction influence of oxytocin and prostaglandinf2α in myometriom layer of uterus to enjection secrection and early contraction of ovary activity.
animal biosystematic
seyedُ pourrabie; Shahrogh Pashaie Rad; Mahbobeh Hajirostamlo; Hasan Baroghie
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Abstract:
Different species of Syrphid flies are beneficial insects in nature and are important in agriculture. During a faunistic study on syrphid flies (Dip.:Syrphidae) on 2011 to 2012 of Jolfa region (East Azarbaijan province), one Merodon Meigen, 1803 species was collected. They were identified ...
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Abstract:
Different species of Syrphid flies are beneficial insects in nature and are important in agriculture. During a faunistic study on syrphid flies (Dip.:Syrphidae) on 2011 to 2012 of Jolfa region (East Azarbaijan province), one Merodon Meigen, 1803 species was collected. They were identified as Merodon latifemoris Radenkovic, based on morphological characters as a new species from Iran region.
In this study, species was collected, and transferred to the Laboratory of Entomology, in Islamic Azad University of Marand and was studied at the morphological character.
The syrphidae important organs such as legs, wings, thorax, abdomen and genitalia were examined. In this research isolated end of the tergit, and transferred in to KOH 10% for 24-12h and then transferred in to alchole 10%, so added one drope glycrin at study time, and was recognized by prestigious keys. Their figures and a simple diagnostic key were provided for their determination in this region.
Animal physiology
Seyed Ebrahim Hosseini; Elham Rezaei; Davood Mehrabani
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Increasing the levels of blood lipids is the most common factors in diabetes that will exacerbate its complications. PJ (Pomegranate juice) is a nutrient with medical purposes. The present study aimed to investigate the effects of pomegranate juice on levels of triglycerides, cholesterol; LDL and HDL ...
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Increasing the levels of blood lipids is the most common factors in diabetes that will exacerbate its complications. PJ (Pomegranate juice) is a nutrient with medical purposes. The present study aimed to investigate the effects of pomegranate juice on levels of triglycerides, cholesterol; LDL and HDL in healthy and diabetic adult male rats were done. In the study, 90 adult male rats weighing 220-200 g were divided into groups of control, diabetic and non-diabetic experimental groups. Each group includes 9 mice. Experimental groups received respectively 1, 2 and 4 ml of pomegranate juice per rat for 21 days. To create diabetes by Intra-peritoneal injection, streptozotocin 60mg/kg was used. At the end of experiment by the phlebotomizing the animal hearts, serum levels of triglycerides, cholesterol, LDL and HDL were measured. Data were analyzed by ANOVA and LSD tests. The results showed that pomegranate juice in doses of 2 and 4 ml caused a significant reduction in triglyceride levels, respectively, in the P
animal biosystematic
azita farashi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Carnivores play great ecological roles in ecosystems because of placing on top of the food pyramid. Food habits of large carnivores are associated with important ecological features such as spatial pattern, movement, habitat selection, social structure, geographical distribution and reproductive success. ...
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Carnivores play great ecological roles in ecosystems because of placing on top of the food pyramid. Food habits of large carnivores are associated with important ecological features such as spatial pattern, movement, habitat selection, social structure, geographical distribution and reproductive success. Accordingly, in this study was used scat analysis to determine the diet of persian leopards in Sarigol National Park using percent of occurrence index. The results showed that diet of leopards in the Sarigol National Park includes a high diversity of wild boar (Sus scrofa), wild goat (Capra aegagrus), wild sheep (Ovis orientalis), tolai hare (Lepus tolai), common fox (Vulpes vulpes), Indian crested porcupine (Hystrix indica), domesticated animals; dog, horse, bird and plant materials. Wild sheeps have the largest share and rabbits and horses have the lowest share in the diet of this species. Undoubtedly, identifying food habits of leopard and maintaining prey populations can improve management programs and conservation status.Keywords: Food pyramid, Scat, Food habits
Animal physiology
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: ...
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Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: In a quasi-experimental pre/post design, twenty two volunteer healthy men (aged 20-30 years and body fat 10-16%) participated in an exhaustive aerobic exercise (Bruce treadmill test). Serum and saliva samples were collected immediately before and after the exercise test. C-reactive protein was determined by immunophotometry and IL-6 was determined by ELISA method. Data were expressed as mean (±SD) and analyzed by t-test and Spearman's correlation coefficient at α≤0.05. Results: The results show that the serum and saliva CRP and IL-6 were significantly increased (P
Omid Tabiee
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
The objective of this paper was to determine species diversity of wintering waterbirds from a ten-year (2002-2011) monitoring in Meyghan wetland. Data for calculating the estimates of biodiversity come from the midwinter census by using a total count from the department of the Markazi province's environment. ...
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The objective of this paper was to determine species diversity of wintering waterbirds from a ten-year (2002-2011) monitoring in Meyghan wetland. Data for calculating the estimates of biodiversity come from the midwinter census by using a total count from the department of the Markazi province's environment. Data analysis for determine species diversity carried out by using Ecological Methodology and SPSS software. During a 10 periods from 2002 to 2011, a total of 134350 wetland birds composed of 56 species from 6 orders and 16 families of the bird fauna of Iran were observed during the course of this study. The most common species counted was the Common Crane (Grus grus) with a total of 54000 individuals. The results indicated that among families of migratory, wintering waterfowl and waders in Meyghan wetland the large family was Gruidae with 40.19 percent abundance and the lowest was Haematopodidae and Burhinidae each one with 0.01 percent. The highest species richness recording in years of 2010 with 37 species in this year. The results showed that in this period wader's fauna with 62.1 percent and waterfowl fauna with 37.9 percent, respectively has the highest and lowest abundances. According to the results of species diversity and evenness the year of 2010 has the highest biodiversity of migratory, wintering waterfowl and wader's fauna in Meyghan wetland and the year of 2002, 2003 and 2008 has the lowest biodiversity (P < 0.05).
Animal physiology
ramesh monagemi; noushin naghsh
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian ...
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Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian C. medica on Hela cell line. After preparation extract and volatile oils, different concentrations of volatile oils of C. medica (0.25-10 μg / ml) and extract of Citrullus Colocynthis (100-1000 μg / ml) were added to Hela cultured cells and incubated for 72 hours. Cell survival was evaluated using MTT-based cytotoxicity assay. The results showed that methanolic extract of Citrullus Colocynthis had not significant cytotoxic effect on Hela cells but Volatile oils of Citrus medica had significant cytotoxic effect on this cells and it’s most compounds was limonene , linalool , linalool acetate and beta - pinene. The findings showed that volatile oils of C. medica had more cytotoxic effect than the Citrullus Colocynthis extract, limonene and linalool of the volatile oil causes this effects.
Biochemistry
reza Haji Hosseini
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum ...
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Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum total protein was measured by the Bradford method.
Results:
The average amount of protein in the serum of exposure to mustard gas (patients) compared with a control group, showed no significant difference (05/0
Biochemistry
Nayere Bahamin; Behzad Sareghi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
In this study, the interaction of various concentrations of Cadmium Sulfate with Peroxidase (E.C 1.11.1.7) was investigated in different temperatures (25-35 °C). For this purpose thermostability, spectrophotometry, spectroflorimetry and kinetics studies were done to obtain thermodynamic parameters including ...
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In this study, the interaction of various concentrations of Cadmium Sulfate with Peroxidase (E.C 1.11.1.7) was investigated in different temperatures (25-35 °C). For this purpose thermostability, spectrophotometry, spectroflorimetry and kinetics studies were done to obtain thermodynamic parameters including ∆G°, ∆H°m, ∆S°m, Tm and also kinetics parameters including Vmax and Km. The results showed that Cadmium Sulfate caused uncompetitive inhibition at different temperatures and increasing of temperature intensified this inhibition effect. Also Cadmium Sulfate was reduced the stability of peroxidase and reduced its Tm. In the spectrophotometric studies the effect of Cadmium Sulfate on the peroxidase in such a way that the absorption in 275nm increased and temperatures increased it too, also the absorption in 404nm (soret bond) decreased and temperature decreased it too. Totally, Cadmium Sulfate ions in a time- and dose- dependent manner and affected by temperature bind to the peroxidase in the heme environment and decrease the thermostability of it, and exert uncompetitive inhibition.
animal biosystematic
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
To identify the species of sea cucumbers belong to Holothuroidea in the intertidal zones of Chabahar Bay, field studies were conducted during one year from November 2008 to September 2009. The area of study was located at the farthest end of southeast of Iran in province of Sistan and Balouchestan ...
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To identify the species of sea cucumbers belong to Holothuroidea in the intertidal zones of Chabahar Bay, field studies were conducted during one year from November 2008 to September 2009. The area of study was located at the farthest end of southeast of Iran in province of Sistan and Balouchestan between 25°16́́ 61̋ and 25°22́ 47 ̋ North, and between 60°39́ 90 ̋ and 60°24́ 38 ̋ East. 5 sampling stations along the shoreline zone was determined based on geomorphological characteristics, habitat and accessibility. Bimonthly Sampling was carried out in the selected stations in complete tide and all samples of each species were collected. Then samples were transferred to laboratory and identified based on extracted ossicles with valid identification keys .the four species of sea cucumbers were identified: Holothuria leucospilota, Holothuria arenicola, Holothuria insignis, Holothuria sp. Which bolnge to family of Holothuroidae and order of Elasipodida. The Maximum frequency of Holothuroidea Was measured in January (2009) in station 4.
animal biosystematic
Articles in Press, Accepted Manuscript, Available Online from 12 November 2014
Abstract
Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters ...
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Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters including shape, size of shell, dextral and sinistral position of the shell, operculum, aperture, radula, mucus colour, respiratory pore, keel, renal ridge and reproduction system. The most and least distribution among identified snail species are 18 and 8 species belong to 4 and 2 stations respectively from Dez river, which they are 20/82 kilometers away from each other. The most commonly distributed family is Thiaridae with 8 species Melanopsispraemorsa ، Melanopsis costata ،Melanopsis doriae Melanopsis nodosa، Melanopsis subtingitana, Melanoides pyramis, Melanoides tuberculata Thiara scabra. Bulinus truncates and Lymnaea truncatula are identified as the most frequent and has medical importance.Melanopsis subtingitana Annandale,1918 and Gyraulus huwaizahensis species are reported for the first time from Iran.
teimour thanha
Articles in Press, Accepted Manuscript, Available Online from 15 May 2017
Abstract
This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal fistula .first, ...
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This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal fistula .first, The chemical properties include: moisture, ash, fat, lactose and protein were measured. Later, using Tris buffer samples were extracted proteins and the extracted proteins were determined electrophoresis. For nylon bag technique, the amounts of 5 gram of whey powder samples were placed inside the bag. Incubation times were: 0, 2, 4, 8, 16, 24, 48 and 72 hours. Gel electrophoresis showed that the major protein in whey powder consisted of β- lactoglobulin .lactalbumin, bovine serum albumin and immunoglobulin. These proteins are respectively 50, 20, 10 and 10 percent of total whey proteins in powder form nylon bag Results showed that at 0, 2, 4 and 48 hours incubation, WP1 and WP2 and 72 hours incubation, WP2 highest amount of protein degradability (p
Articles in Press, Accepted Manuscript, Available Online from 13 March 2018
Abstract
Abstract
The bilateral relationship between Ventro Medial Nucleus and dopaminergic system may play an important role in the secretion of the gastrointestinal exocrine glands, nutritional behaviors and habits. The aim of this study was to investigate the role of dopaminergic receptors in regulation of ...
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Abstract
The bilateral relationship between Ventro Medial Nucleus and dopaminergic system may play an important role in the secretion of the gastrointestinal exocrine glands, nutritional behaviors and habits. The aim of this study was to investigate the role of dopaminergic receptors in regulation of the exocrine glands and to find out whether the change in saliva secretion is the primary effects of central nervous system or there are subsequences of taking food, lick, swallowing and digestion in the mouth. Ninety rats were stereotaxically implanted under urethane anesthesia. The rats were divided into 9 groups: one control group, one fasted control group, two sham groups given surgery and surgery plus medicine solvents, one SCH23390 group, one bromocriptine group, one mixed group: SCH23390 along with bromocriptine, one pilocarpine and a mixed group: bromocriptine along with pilocarpine. In this study, saliva secretion was gathered from submandibular glands by cannulas and measures then volume of secretion by using SPSS program the groups were compared (Test Anova). The saliva secretion was not significantly different between all groups except SCH23390 group, pilocarpine, and both mixed groups which showed significant differences in the saliva secretion. This study showed that SCH23390 (7.5 μg) could increase saliva secretion. Bromocriptine could not affect saliva secretion. Bromocriptine along with pilocarpine significantly decreased saliva secretion. However, this agonist together with SCH23390 significantly increased saliva secretion. So the dopaminergic system of the Ventro Medial Nucleus has a considerable effect on the secretion of the exocrine glands in the digestive system.
Articles in Press, Accepted Manuscript, Available Online from 29 April 2018
animal biosystematic
Soheil Eagderi
Abstract
This study was conducted to survey the morphological variation in four populations of the Urmia kingfish (Alburnus atropatenae) using geometric morphometric technique. A total number of 163 specimens were collected from four rivers of the Urmia Lake basin including the Baneh, Saghezchai, Siminehrood ...
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This study was conducted to survey the morphological variation in four populations of the Urmia kingfish (Alburnus atropatenae) using geometric morphometric technique. A total number of 163 specimens were collected from four rivers of the Urmia Lake basin including the Baneh, Saghezchai, Siminehrood and Zarinehrood by electrofishing. The left side of specimens were photographed using a digital camera. Then fifteen homologous landmark points were selected and digitized on 2D pictures using tpsDig2 software. Landmark data after GPA, analyzed using PCA, CVA with obtained P-value from permutation test and cluster analysis. The patterns of population’s body shape were illustrated in relation to consensus configuration of all specimens. The results showed a significant differences between the body shape of studied populations. Based on cluster analysis, the studied populations according to their body shape were divided into Baneh clad and Saghezchai, Siminehrood and Zarinehrood clade. The Baneh, Saghezchai, and Siminehrood populations have deeper body and are distinguishable due to smaller head, posterior position of eye and shorter base of dorsal fin, respectively and Zarinehrood population was differentiable due to a more fusiform body shape and less body depth. These morphological differences can display ongoing evolutionary trends of studied populations as result of environmental conditions of their habitats and geographical isolation.
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