Abstract
Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; the released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometric analysis were done for CD105, CD90, CD146, CD31 and CD34 at third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated at the third passage after 21 days of induction.The flowcytometry analysis showed that EnSCs were positive for CD90, CD105, CD146 and were negative for CD31, CD34. Moreover we show that the hEnSCs can differentiate in osteocyte and adipocyte, as confirmed by alizarin Red and oil-Red O staining. These differentiated cells into neuron and endothelial express related markers including Nestin, MAP2 and CD31. Results show that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.
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