Fisheries
Bita Seraj; Mesbah Mehrzad; Paria Akbari
Abstract
In this study, in addition to the LC50 and the maximum allowable concentration, effects of silver nanoparticles on several of immune and blood serum enzymes parameters of Barbus grypus were determined For this purpose, 240 shirbut, to investigate the effects of sub lethal toxicity were divided as follows-control ...
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In this study, in addition to the LC50 and the maximum allowable concentration, effects of silver nanoparticles on several of immune and blood serum enzymes parameters of Barbus grypus were determined For this purpose, 240 shirbut, to investigate the effects of sub lethal toxicity were divided as follows-control treatment (G1), the concentration LC50 1% (0.012 mg/L AgNP) (G2), concentration LC50 2% (0.025 mg/L AgNP) (G3) and concentration LC50 4% (0.05 MG/L AgNP) (G4) and concentration LC50 8% (0.1 Mg/ L AgNP) (G5). Then on days zero, 7, 14 and 21 immune responses and serum enzymes (ALT, LDH, SGOP, SGPT) in Barbus grypus were measured. In the study, WBC counts increased significantly (P<0.05) in G2, G3, G4 and G5 treatments compared to control (G1). Serum anti-bacterial activity was enhanced in G3 in twenty first day and G4 and G5 in 14th and 21s days in compared to control. Serum lysozyme activity enhanced significantly (P<0.05) in (G4) in 14th and (G5) 14th and 21s in days compared to control. ALT and LDH Level decreased significantly in (G2), (G3), (G4) and (G5) treatments in 21s day compared to control. SGPT level decreased significantly in (G2), (G3), (G4) and (G5) in 21s day compared to control. SGOT level decreased significantly in (G3), (G4) and (G5) treatments in 21s day compared to control. According to the results of this study concentrations toxicity of nano silver increased immune responses and serum enzymes.
Maryam Zobeidy nezhad; Mostafa Ghaffari; Ali Taheri
Volume 7, Issue 1 , September 2018, Pages 17-25
Abstract
Abstract Nowadays due to the emergence of resistant forms of pathogenic bacteria, discovering new antimicrobial substances from marine natural resources is considered very important. This study aims to investigate the antibacterial activity of methanolic extract of brown algae against some Gram negative ...
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Abstract Nowadays due to the emergence of resistant forms of pathogenic bacteria, discovering new antimicrobial substances from marine natural resources is considered very important. This study aims to investigate the antibacterial activity of methanolic extract of brown algae against some Gram negative bacteria. After sampling and transferring the samples to the laboratory, algae samples were washed, dried and pulverized; then, the extraction was conducted using methanol solvent. Methanolic extracts of algae (padina gymnospora) were prepared in four concentrations of 10 to 80 mg/ml and were tested against 4 strains of Gram negative bacteria Vibrio cholerae, Morganella morganii, Proteus mirabilisand Proteus vulgaris using disc diffusion technique and inhibition growth zone measurement. The results revealed that P. vulgaris, with growth inhibition zone diameter of 11.23±0.25 mm in 80 mg/ml concentration, showed the maximum sensitivity to algae extract. This value had a significant difference compared to reference antibiotics of gentamycin and neomycin and all other concentrations (ppadina gymnospora, can be considered a potential resource of antimicrobial compounds aiming to replace chemical medicines.
Namdar Yousofvand; Vahid Hasanvand
Volume 7, Issue 1 , September 2018, Pages 27-33
Abstract
Abstract Many of the medicinal herbs have shown to be effective in reducing the incidence of diabetes complications (including hematocrit), which garlic is one of these herbs. 21 adult Wistar male rats were divided into 3 groups (n=7), which include: normal control group that received normal water and ...
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Abstract Many of the medicinal herbs have shown to be effective in reducing the incidence of diabetes complications (including hematocrit), which garlic is one of these herbs. 21 adult Wistar male rats were divided into 3 groups (n=7), which include: normal control group that received normal water and food for 45 days and injected normal saline on 15th day. Control (diabetic by STZ at 40 mg/kg dose on 15th day) and the prevention group by garlic flower extract at a dose of 360 mg/Lit in drinking water received a treatment period for 15 days before diabetes. One dose of STZ 40mg/kg was injected intraperitoneally. The hematocrit of blood samples was measured, using the ABACUS-c Diatron device (Austria). Hematocrit showed a significant increase (p
azita farashi; Mohammad Sarbaz; Ali Khani
Volume 7, Issue 1 , September 2018, Pages 35-44
Abstract
Abstract Khorasan Razavi province, including important habitats for marbled polecat (Vormela peregusna) in Iran. in this study, we used maximum entropy method (Maxent) for habitat modeling in the province. This method is an effective approach for habitat modelling with presence points. In this study, ...
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Abstract Khorasan Razavi province, including important habitats for marbled polecat (Vormela peregusna) in Iran. in this study, we used maximum entropy method (Maxent) for habitat modeling in the province. This method is an effective approach for habitat modelling with presence points. In this study, 25 points were used and 89 percent accuracy was achieved. Based on the results, the northern and northwestern regions of the province are more suitable for the species. According to the study, 6% of the whole province is suitable for the species. The results also showed that the suitable habitats areas overlap with roads and in addition to roads with human settlements such as towns and villages also showed a positive relation and in according to the responses curves, with increasing of distance of the areas, decreasing habitat suitability. And also, forest areas and rivers have a positive effect on habitat suitability. According most environmental degradation is about habitats, so we must try to survey and management these areas appropriately.
Nassir Niamaimandi; Azizollah Mahdevi
Volume 7, Issue 1 , September 2018, Pages 45-53
Abstract
Abstract In the Persian Gulf waters, the sciaenid species, Otolithes ruber, plays an important role in the commercial fishery and to this reason, the resources are under catch pressure. Stock assessment and population dynamics parameters of this species were studied in Bushehr and Khozestan waters from ...
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Abstract In the Persian Gulf waters, the sciaenid species, Otolithes ruber, plays an important role in the commercial fishery and to this reason, the resources are under catch pressure. Stock assessment and population dynamics parameters of this species were studied in Bushehr and Khozestan waters from April 2012 to March 2013. In the present study, 4203 of specimens obtained from gillnet landing places and length frequency data (fork length) were recorded. Length frequency ranging from 11 to 50 cm. The parameters of the von Bertalanffy growth equation (males and females combined) for asymptotic length (L∞) was 52.5 cm and instantaneous growth coefficient (K) was 1.1 year−1. The instantaneous rates of total mortality (Z), fishing mortality (F) and natural mortality (M) were 3.42, 1.85 and 1.57 per year, respectively. Estimated parameters of precautionary target (Fopt) and limit (Flimit) biological reference points were 0.79 and 1.04 respectively. The values of two parameters Fopt and Flimit were lower than fishing mortality (F) that showed this species is over exploited in the Bushehr and Khozestan waters by gillnet fishery.
Amir Arasateh; Samira Vaziri; Mohammad Fazilati; Habib-ollah Nazem
Volume 7, Issue 1 , September 2018, Pages 55-72
Abstract
Abstract Amyloids are created from various proteins in denaturant conditions. Highly active surface, makes these materials suitable for enzyme immobilization. The aim of this research was to study the production of amyloid fibrils and investigate the possibility of using them as a matrix for enzyme immobilization. ...
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Abstract Amyloids are created from various proteins in denaturant conditions. Highly active surface, makes these materials suitable for enzyme immobilization. The aim of this research was to study the production of amyloid fibrils and investigate the possibility of using them as a matrix for enzyme immobilization. Response surface methodology (RSM) was used to generate the maximum amyloid content. The results of the Congored spectrometry and circular dichroism (CD) were analyzed by Design Expert 7 software (Trial version) and transmission electron microscopy was used to confirm the presence of amyloid fibrils. The immobilization was done by creating glutaraldehyde mediated crosslink of enzyme on the amyloid fibrils and kinetic factors including activity, specific activity, optimal temperature and pH and thermal stability were compared with the free enzyme. The highest amyloid content was obtained after 72.6 hours agitation of bovine serum albumin at 4.35 mg.ml-1 in mixed citrate–phosphate buffer pH=4.49 at 80 ºC and immobilized lipase was improved relative to free lipase in the case of activity, specific activity, Km and Vmax, optimal temperature and pH and thermal stability at 40 ºC. Amyloid fibrils as a rich material of chemical groups can be suitable for stabilization. This protein matrix can be a good inVivo candidate as a bio–compatible scaffold for enzyme immobilization. Amyloids with a diameter less than 100 nanometers, as new nano–materials, not only increase the stability of lipase but also enhance other kinetic properties as a new nano–matrix.
Amir Dehghani Samani; Yaser Pirali; Mehdi Salimi; Nader Ahmadi Saleh Baberi; Habib Allah Rashidzadeh
Volume 7, Issue 1 , September 2018, Pages 73-80
Abstract
Abstract Ticks, as usual groups of ecto-parasites, have abundant importance in medicine due to their ability in conservation and transmission of important pathogens like zoonotic pathogens. They choose different species as host including; mammals, reptiles and birds. Hedgehog, a mammal species which ...
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Abstract Ticks, as usual groups of ecto-parasites, have abundant importance in medicine due to their ability in conservation and transmission of important pathogens like zoonotic pathogens. They choose different species as host including; mammals, reptiles and birds. Hedgehog, a mammal species which is related to humans and animals, can be infested by ticks. Aim of current study is identification and description of ticks fauna in native hedgehogs located in central parts of Iran. Six hedgehogs were collected from rural areas in Najafabad (Isfahan province) and 10 ticks were collected from different part of their bodies in examination. Ticks were temporary preserved in solution contained 95% Etanol 70° plus 5% glycerin and then were cleared via KOH, mounted and observed by light microscope and their identifications were confirmed via wallker tick systematic keys. Ticks were identified as Rhipicephalus appendiculatus, Rhipicephalusturanicus and Haemaphysalis concinna. This study reports the hedgehog's infestation by Haemaphysalis concinna for the first time in Iran. Recognition of new hosts for ticks and dimension of their dispersion are first steps in order to solve their problems. Presence of ticks on new hosts and their resistance against pesticides are also important subjects that must be considered more than past.
Sima Nasri
Volume 7, Issue 1 , September 2018, Pages 81-88
Abstract
Abstract Because of many side effects of chemical drugs, herbal medicines have been considered for cancer treatment. Hulthemiapersica has five phenolic antioxidants.Therefore, in the present study, its anticancer activity in Helacells was studied in comparison with healthy Hek cells. After collecting ...
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Abstract Because of many side effects of chemical drugs, herbal medicines have been considered for cancer treatment. Hulthemiapersica has five phenolic antioxidants.Therefore, in the present study, its anticancer activity in Helacells was studied in comparison with healthy Hek cells. After collecting the plant, its aerial parts were separated and extracted by percolation method. Hela and Hek cell lines were placed in plates of 96 houses with various concentrations of 8.7, 15.625, 31.25, 62.5, 125, 250, 500, 1000µ g/ mlfor 24, 48 and 72 hours, and the percentage of cell viability Measured by MTT test. Plant extract has no inhibitory effect on Hek cells. However, Hulthemiapersica extract decreases viability rate of the Hela cells at doses of 125, 62.5, 31.25, 15.6, and 7.8 in 24 hours,at all doses in 48 hours and at doses of 1000, 500, 250, 125, 62.5, 31.25 in 72 hours compared to the control group (P<0.05).
Saeid Mohammadzadeh; Arash Azarfar; Mahdiyeh Gholamipour
Volume 7, Issue 1 , September 2018, Pages 89-96
Abstract
Abstract The Effects of low frequency of electromagnetic fields in albumin quality, blood parameter, and hatchability on one day chicken broiler. With the spread of technology, organisms with high diversity are exposed to electromagnetic fields (EMF). In order to study the effect of low frequency ...
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Abstract The Effects of low frequency of electromagnetic fields in albumin quality, blood parameter, and hatchability on one day chicken broiler. With the spread of technology, organisms with high diversity are exposed to electromagnetic fields (EMF). In order to study the effect of low frequency of electromagnetic fields during storage of eggs, 138 fertile eggs (Ross strain) were selected. Eggs were exposed to 50-Hz electromagnetic field with 50 HZ frequency and 5.5 MT in different time; (control), 8, 16, 24, 32 and 64 hours, with six replications. Duration of electromagnetic field weren’t effect on albumin quality, Haugh unit, cholesterol, triglycerides and total protein level of serum significantly (P
Hassan Ghahari
Volume 7, Issue 1 , September 2018, Pages 97-106
Abstract
Abstract The ground beetles (Coleoptera: Carabidae) are powerful predators and have important role in biological control of agricultural pests. The fauna of these beneficial insects was studied in fruit orchards and rice fields of Mazandaran province during 2015-2016. In total, 22 species within 16 genera ...
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Abstract The ground beetles (Coleoptera: Carabidae) are powerful predators and have important role in biological control of agricultural pests. The fauna of these beneficial insects was studied in fruit orchards and rice fields of Mazandaran province during 2015-2016. In total, 22 species within 16 genera and 10 subfamilies were identified in different regions of Mazandaran. The samplings indicated that the highest species diversity was observed in Amol and Qaemshahr, and the lowest species diversity in Babolsar; also the highest population density was observed in Qaemshahr and Behshahr, and the lowest one in Babolsar and Noor regions. Among the studied species, Harpalus griseus (Panzer, 1797) had the highest distribution and Acinopus megacephalus (Rossi, 1794), Bembidion menetriesi Kolenati, 1845, Carabus roseni Reitter, 1897, Chlaenius steveni Quensel, 1806 and Calosoma maderae dsungaricum Gebler, 1833 the lowest one. Also, the highest population density was obtained for H. griseus and Harpalus fuscicornis Menetries, 1832 and the lowest one for A. megacephalus, B. menetriesi, C. steveni, Cicindela rhodoterena Tschitschherine, 1903, C. maderae dsungaricum and Zabrus spectabilis Hampe, 1852, respectively. The results indicate that Harpalus griseus and Harpalus fuscicornis are the dominant species in the most sampled regions of Mazandaran province.
Yazdan Keivany; Atta Mouludi Saleh; Seyed Amir Hossein Jalali
Volume 7, Issue 1 , September 2018, Pages 107-118
Abstract
Abstract In order to evaluate the biometry of chub (Squalius namak, Khaefi et al., 2016) populations in Khaznagh, Ghinercheh, Gharechae, Jajrud and Ghomrud rivers of Lake Namak Basin, 129 specimens were captured by a seine nets in 2010-2011. After anesthetizing in 1% clove oil solution and fixing in ...
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Abstract In order to evaluate the biometry of chub (Squalius namak, Khaefi et al., 2016) populations in Khaznagh, Ghinercheh, Gharechae, Jajrud and Ghomrud rivers of Lake Namak Basin, 129 specimens were captured by a seine nets in 2010-2011. After anesthetizing in 1% clove oil solution and fixing in 10% neutralized formalin, specimens were transferred to the laboratory for further studies. Some 14 meristic characters were counted under a stereomicroscope. To extract data for morphometric characteristics, 19 distances were measured on images in ImageJ software. To reduce the effects of allometric growth, morphometric data were standardized. To analyze the differences among the populations, Kolmogorov-Smirnov, Kruskal-Wallis, ANOVA, Duncan test, PCA, CVA and Cluster analyses were used. The morphometric characteristics separated Gharachai population from Khaznagh and Qomrud in PCA and CVA Analyses. But meristics were overlapping with each other in the studied populations, and there was no significant differences among them. The result showed that some morphometric characteristics are well able to distinguish some populations of S. namak.
Saeed Esmaeili-Mahani2; Mehri Bahaaddini; Saeed Khatamsaz; Mehdi Abbasnejad; Maryam Raoof
Volume 7, Issue 1 , September 2018, Pages 119-132
Abstract
Abstract Orexin A and orexin B are hypothalamic neuropeptides that have critical roles in the regulation of physiological responses such as pain. C-fos is neural activity markers in painful situations. This study was designed to evaluate the role of trigeminal nucleus caudalis (TNC) orexin 1 receptor ...
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Abstract Orexin A and orexin B are hypothalamic neuropeptides that have critical roles in the regulation of physiological responses such as pain. C-fos is neural activity markers in painful situations. This study was designed to evaluate the role of trigeminal nucleus caudalis (TNC) orexin 1 receptor on capsaicin-induced orofacial pain and c-fos expression in TNC. In this experimental study, 70 male Wistar rats (200-250 g) were randomly allocated into 10 groups: control, pain, sham surgery, sham capsaicin, surgery and pain, sham orexin A and pain, orexin A (50 pM/rat) and pain, sham SB-334867 and pain, SB-334867 (40 nM/rat) as antagonist and pain, orexin A plus SB-334867 and pain. Following cannula implantation and recovery period, the drugs were administration 20 min before capsaicin injection. C-fos expression in TNC was evaluated using immunohistochemistry. The capsaicin-injected rats showed a significant pain behavior and expression c-fos (p
Tohid Vahdatpour; Yahya Ebrahimnezhad; Hosein Cheraghi; Morteza Hesari
Volume 7, Issue 1 , September 2018, Pages 133-141
Abstract
Abstract The aim of the present study was to investigate the effects of theophylline consumption on the electrocardiography indicators and performance traits of Japanese quails. The experimental diets including control (basal diet), and experimental diets containing of theophylline in levels of: 0.01, ...
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Abstract The aim of the present study was to investigate the effects of theophylline consumption on the electrocardiography indicators and performance traits of Japanese quails. The experimental diets including control (basal diet), and experimental diets containing of theophylline in levels of: 0.01, 0.02, 0.04 and 0.08 percentage of basal diet that supply almost amount 10, 20, 40 and 80 mg/kg of the body weight, respectively. The result showed that the feed conversion ratio in quails fed by theophylline was significant (p
Behzad Taneh; behrooz abtahi; rajab mohamad nazari
Abstract
In vitro assessment of final oocyte maturation index (GVBD) in Persian Sturgeon and broodstock selectionFinal oocyte maturation index (GVBD) inin vitro for selection of Persian sturgeon Broodstock, Acipenserpersicus, was studied on 10 females captured from the Caspian Sea during reproduction season. ...
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In vitro assessment of final oocyte maturation index (GVBD) in Persian Sturgeon and broodstock selectionFinal oocyte maturation index (GVBD) inin vitro for selection of Persian sturgeon Broodstock, Acipenserpersicus, was studied on 10 females captured from the Caspian Sea during reproduction season. About 150 oocytes from every broodstock, taken by hollow probe, were incubated in incubation media of RM2 containing 10 µg/l of progesterone for induction ofthe final oocyte maturation andassessment of GVBDindex. Results indicated that significant differences in GVBD of thein vitro and in vivo were observed (P<0.01). However, Broodstockes with rate of GVBD inin vitro higher than %50 had a significantly better ovulation, fertilization and incubation rates (P<0.05), from those with a GVBD lower than %50. It could be concluded that use from method of assessment of GVBD index under in vitro circumstance was preferable to nucleus polarization index for selection of suitable broodstock.Keywords:Acipenserpersicus, in vitro, GVBD,final oocyte maturation,Broodstock Selection
