In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Document Type : Article


1 Associate Professor, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

2 Professor, Department of Biology, College of Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran and Department of Biology, College of Science, Shiraz Branch , Islamic Azad University, Shiraz, Iran.

3 Associate Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran and Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.



Orexin A and orexin B are hypothalamic neuropeptides that have critical roles in the regulation of physiological responses such as pain. C-fos is neural activity markers in painful situations. This study was designed to evaluate the role of trigeminal nucleus caudalis (TNC) orexin 1 receptor on capsaicin-induced orofacial pain and c-fos expression in TNC. In this experimental study, 70 male Wistar rats (200-250 g) were randomly allocated into 10 groups: control, pain, sham surgery, sham capsaicin, surgery and pain, sham orexin A and pain, orexin A (50 pM/rat) and pain, sham SB-334867 and pain, SB-334867 (40 nM/rat) as antagonist­ and pain, orexin A plus SB-334867 and pain. Following cannula implantation and recovery period, the drugs were administration 20 min before capsaicin injection. C-fos expression in TNC was evaluated using immunohistochemistry. The capsaicin-injected rats showed a significant pain behavior and expression c-fos (p


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