Authors

• Behzad Taneh ¹
• behrooz abtahi ²
• rajab mohamad nazari ³

¹ assistant of payame noor University

² assistantprof. of shahidbeheshti university

³ ShahidRajaei Sturgeon fish farm - Sari

Abstract

In vitro assessment of final oocyte maturation index (GVBD) in Persian Sturgeon and broodstock selection
Final oocyte maturation index (GVBD) inin vitro for selection of Persian sturgeon Broodstock, Acipenserpersicus, was studied on 10 females captured from the Caspian Sea during reproduction season. About 150 oocytes from every broodstock, taken by hollow probe, were incubated in incubation media of RM2 containing 10 µg/l of progesterone for induction ofthe final oocyte maturation andassessment of GVBDindex. Results indicated that significant differences in GVBD of thein vitro and in vivo were observed (P<0.01). However, Broodstockes with rate of GVBD inin vitro higher than %50 had a significantly better ovulation, fertilization and incubation rates (P<0.05), from those with a GVBD lower than %50. It could be concluded that use from method of assessment of GVBD index under in vitro circumstance was preferable to nucleus polarization index for selection of suitable broodstock.
Keywords:Acipenserpersicus, in vitro, GVBD,final oocyte maturation,Broodstock Selection

Keywords

• Acipenserpersicus
• in vitro
• GVBD
• final oocyte maturation
• Broodstock Selection