Davoud Fazli; Aliakbar Malekirad
Abstract
Reactive oxygen species (ROS) are cytotoxic agents that lead to significant oxidative damage. Bisphenol A (BPA) is a contaminant toxic, oxidative and estrogenic effects. Purslane is a promising natural product, which could be useful for the prevention of chronic diseases caused by oxidative stress. In ...
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Reactive oxygen species (ROS) are cytotoxic agents that lead to significant oxidative damage. Bisphenol A (BPA) is a contaminant toxic, oxidative and estrogenic effects. Purslane is a promising natural product, which could be useful for the prevention of chronic diseases caused by oxidative stress. In this study 49 adult male rats (250 10g) were divided randomly in 7 groups (n=7): Control, Sham (500 ug corn oil\Kg BW), Toxin group (500ug BPA\kg BW), Positive control (100mg purslane hydroalcoholic extract\kg BW) and 3 purslane treated groups (500ug BPA + 50,100&150 mg purslane\kg BW). All injections are done by i.p rout in 14 consequently days. At the end of administration time (14 day) all animals were anesthetized with Chloral hydrate and sacrificed, then liver samples isolated to preparing tissue homogenates for measure antioxidant enzymes including SOD, CAT, TAC, GPX and MDA. Data are analyzed in SPSS statistical software by one way ANOVA with Tukey posttest. Analyzed data showed that MDA, TAC, CAT, GSH and GPX measures are modified in purslane treated groups compared to the bisphenol A group which indicates that the portulaca oleracea extract can improve the hepatotoxicity of bisphenol A induced.
Biochemistry
Milad Saffary; Zolfaghar Lotfi; Amir Arasteh
Abstract
Aloysia citrodora is very important in Iranian traditional medicine. The aim of this study was to produce silver nanoparticles with aqueous extract of A. citrodora and investigate its antioxidant and inhibitory effects on the amyloid nanofibers from BSA as model protein. For synthesis of silver nanoparticles, ...
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Aloysia citrodora is very important in Iranian traditional medicine. The aim of this study was to produce silver nanoparticles with aqueous extract of A. citrodora and investigate its antioxidant and inhibitory effects on the amyloid nanofibers from BSA as model protein. For synthesis of silver nanoparticles, aqueous A. citrodora extract was prepared and one mM silver nitrate was added to solution. To confirm the presence and determine the size of silver nanoparticles, UV-Vis spectroscopy and TEM were used. The antioxidant effect of the extract was evaluated by DPPH and the production of amyloid fibers was measured by Congored absorption method. The toxicity effect of nanoparticles on neuroblasts was investigated by MTT method and the inhibitory properties of the extract on amyloid production were investigated by TEM. Caryophyllene (7.66%), phytol (7.47%) and limonene (2.68%) were the most abundant compounds. TEM also confirmed spherical crystalline nanoparticles with a size between 30–70 nm. By increasing the concentration of the extract, the antioxidant properties of nanoparticles increased, so that 97.35% of DPPH free radicals were inhibited at a concentration of 1 mg/ml. The highest inhibition of amyloid fibrils production was observed at concentration of 2 mg/ml by 96%, and the highest level of cytotoxicity at a concentration of 2 and with an IC50 of 2.25 mg/ml. The results showed that nanoparticles from A. citrodora leaves with anti–amyloid, antioxidant and anticancer properties can be considered as a new bio–nanomaterial in reducing Alzheimer's and cancer complications.
Zahra Khodabandeh; Sanaz Alaee; Mahintaj Dara; Maryam Davari; Azizollah Bakhtari
Abstract
Sodium polyacrylate is a material with a high potential for water and moisture absorption. In this study, the effects of this material on the rate of embryo development and the expression of apoptosis-related and antioxidants genes in the blastocyst was evaluated. Adult female mice were superovulated ...
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Sodium polyacrylate is a material with a high potential for water and moisture absorption. In this study, the effects of this material on the rate of embryo development and the expression of apoptosis-related and antioxidants genes in the blastocyst was evaluated. Adult female mice were superovulated by injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) and after placing beside adult male mice, zygotes were harvested from oviducts and transferred into media containing 0, 5, 25, and 50 μg/ml sodium polyacrylate. Zygotes were cultured towards the blastocyst stage and the rate of embryo development was assessed. Expression of intended genes were evaluated by real time RT-PCR. One-way analysis of variance (ANOVA) and Duncan's post hoc toast were used to determine the differences between the means of the groups. The rate of blastocysts was significantly lower in 50 μg/ml compared to the control group (P<0.05). The expression of Bcl-2 increased significantly in 5 μg/ml in comparison to the control group and decreased significantly in 50 μg/ml compared to 5 and 25 μg/ml (P<0.05). The expression of Bax/Bcl-2 and Caspase-3 in 50 μg/ml increased significantly in comparison to 0, 5, and 25 μg/ml (P<0.05). Therefore, the high concentration of sodium polyacrylate has an adverse effect on the embryo through apoptosis system.
Mokhtar Fathi; Mohamad Heydari; Mehran Mohamadikhah
Abstract
The present study was performed to investigate the effect of furosemide on oxidative stress, mortality due to ascites and some blood parameters in broiler chickens. A total of 450 Ross 308 days-old chicks were assigned to 3 groups (control and two treatments of 30 and 60 ppm zamide) in a completely randomized ...
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The present study was performed to investigate the effect of furosemide on oxidative stress, mortality due to ascites and some blood parameters in broiler chickens. A total of 450 Ross 308 days-old chicks were assigned to 3 groups (control and two treatments of 30 and 60 ppm zamide) in a completely randomized design with 5 replications and 30 chicks per replicate. The birds were subjected to a special cold temperature program to induce ascites. Blood parameters: red blood cell count, hematocrit, hemoglobin, white blood cells, activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatine kinase and oxidative stress parameters such as; Plasma antioxidant capacity, plasma malondialdehyde, glutathione peroxidase and superoxide dismutase activity, glucose, protein, triglyceride, cholesterol and high-density lipoprotein were measured. Performance parameters of body weight gain, feed intake and feed conversion ratio were also evaluated. Deaths were recorded daily and described to differentiate the cause of death and determine ascites. The results showed that administration of 60 ppm of furosemide significantly reduced weight and feed intake as well as improved feed conversion ratio (P<0.05). In addition, furosemide, significantly decreased ascites index and ascites losses (P<0.05). Also, level of 60 ppm furosemide, increased white blood cells, plasma antioxidant capacity, glutathione-peroxidase activity). Decreased plasma protein, malondialdehyde and activity of plasma aspartate, aminotransferase, alkaline phosphatase, and creatinine kinase (P<0.05). The results of this research show that the level of 60 ppm Furosemide can reduce the problems caused by high blood pressure in birds with this metabolic syndrome by improving its antioxidant status.
Nahid Ghaed Amini; Mohammad Fazilati; Saeid Habib-Allahi; Habib-Allah Nazem; Seyed Hossein Hejazi
Abstract
Despite the many chemical drugs available, the prevalence and mortality rate of melanoma is high. Therefore, the use of drugs of natural origin with lower cost and higher efficiency can be a good way to save patients. Therefore, in this study, the main purpose of this study was to evaluate the effects ...
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Despite the many chemical drugs available, the prevalence and mortality rate of melanoma is high. Therefore, the use of drugs of natural origin with lower cost and higher efficiency can be a good way to save patients. Therefore, in this study, the main purpose of this study was to evaluate the effects of Moringa extract on melanoma. Hydroalcoholic extract of Moringa leaves was prepared. After examining the total phenolic and flavonoid content of the extract, their type was confirmed by HPLC and spectrophotometer methods. The antioxidant power was measured by DPPH free radical scavenging and FRPA methods. Then the tumor was induced in C57BL6 mice and then the effects of the extract were evaluated on B16F10 cell line and tumor mice. The total flavonoid content was 60.65±1.75 mg quercetin acid per gram of dry extract and the total phenol content was 20.25±1.23 mg gallic acid per gram of dry extract. Three phenolic and flavonoid compounds with antioxidant properties identified include quercetin, gallic acid, and caffeic acid. The free radical scavenging power and the reducing power of the extract increased with increasing the concentration. The IC50 of moringa extract was 73 μM/mol. The tumor volume was significantly reduced by different doses of the extract in two weeks. The results of present study show the positive effect of Moringa leaves extract in reducing the survival rate of B16F10 melanoma cancer cells and tumor volume in mice.
Leyla Najafi; Mohammad Fazilati; Hossein Salavati
Abstract
C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various ...
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C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes.
Fereshteh Fallah Digsara; Amir Arasteh
Abstract
Soybean (Glycine max) is a plant of the legume family and one of the most important oil plants. Soybean is rich in various proteins and phytochemicals, especially isoflavones. The aim of this study was to investigate the antioxidant and inhibitory effects of hydro-alcoholic extract of soybean seed on ...
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Soybean (Glycine max) is a plant of the legume family and one of the most important oil plants. Soybean is rich in various proteins and phytochemicals, especially isoflavones. The aim of this study was to investigate the antioxidant and inhibitory effects of hydro-alcoholic extract of soybean seed on acetyl cholinesterase and production of amyloid nanobiofibrils in bovine serum albumin protein as a model protein. First, the yellow soybean seeds was powdered, then its hydro-alcoholic extract was prepared. The extracts composition were obtained by GC-MS spectroscopy. Anti-Alzheimer's effects were performed by measuring antioxidant activity by DPPH method, Inhibition of acetylcholinesterase by ellman method and inhibition of the production of amyloid nanobiofibrils by congored absorption method. Transmission electron microscopy was used to confirm the presence of amyloid fibrils. The presence of genistein active component in yellow soybean seed extract was confirmed by 2.97% and palmitic and linoleic acids by 3.11 and 10.69%, respectively. Investigation of antioxidant activity of soybean extract in different doses showed that with increasing the concentration of the extract, the percentage of antioxidant activity also increases. Investigation of acetylcholinesterase inhibition using a substrate analogue called acetylthiocholine iodide (ATCI) showed that with increasing the concentration of the extract, the amount of enzyme inhibition increases. The amounts of extract at high concentrations reduced the production of amyloid nanofibrils. This was confirmed by electron microscope images.Yellow soybeans, with their good antioxidant and inhibitory effects on the acetylcholinesterase enzyme, can be a good candidate for reducing the side effects of Alzheimer's.
Shadan Golandam; Saleh Tabatabaei Vakili; Khalil Mirzadeh
Abstract
The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint ...
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The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint extract. At different storage times of diluted semen containing treatments (zero, 24, 48 and 72 hours) in liquid condition, semen quality parameters were evaluated. Immediately after sperm collection, the lowest total motility and sperm viability belonged to the level of 200 peppermint extract (P<0.05). At 24 hours, levels 150 and 200 of extract increased the rate of morphological abnormalities of sperms. At this time, the lowest level of sperm plasma membrane integrity was related to the level of 200 peppermint extracts (P<0.05). 48 hours after sperm storage, the highest total motility, viability and plasma membrane integrity of sperms were belonged to the control group (P<0.05). 72 hours after semen storage, no statistically significant differences were found among treatments for all sperm quality parameters. At this time, the highest concentration of malondialdehyde (MDA) in seminal plasma was related to the level of 100 μl/ml peppermint extract (P<0.05). In general, by adding different levels of peppermint extract to the diluent and maintaining the diluted semen of the Arabi ram in a liquid state at 5°C, the concentrations used of peppermint extract not only did not improve the qualitative parameters of the sperm, it also had a devastating effect.
Mokhtar Fathi; Shahriar Saeidian; Kobra Varmaghani
Abstract
A study was conducted to evaluate the antioxidant effects of selenium nanoparticles and sodium selenite on the antioxidant status, enzymatic activity and plasma lipid parameters of Pullet Chickswith 1050 birds in 7 treatments for 6 weeks. The treatments included three levels of 0.15, 0.3 and 0.6 (ppm) ...
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A study was conducted to evaluate the antioxidant effects of selenium nanoparticles and sodium selenite on the antioxidant status, enzymatic activity and plasma lipid parameters of Pullet Chickswith 1050 birds in 7 treatments for 6 weeks. The treatments included three levels of 0.15, 0.3 and 0.6 (ppm) sodium selenite and nano selenium. The antioxidant indices measured in the heart, liver and plasma include glutathione peroxidase (GPx), malondialdehyde (MDA) and total antioxidant capacity (TAS). Activity of alanine aminotransferase (ALT) enzymes, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were measured and lipid parameters including total triglyceride, total cholesterol and plasma LDL were determined. The results showed that sodium selenite and selenium nanoparticles significantly increased the activity of glutathione peroxidase and reduced levels of malondialdehyde in the plasma, heart and liver. The highest antioxidant effect was related to 0.6 ppm sodium selenium and 0.3 ppm selenium nanoparticles. There was no significant effect on total antioxidant capacity of plasma, heart and liver in experimental group (P<0.05) .There was no significant effect of experimental treatments on plasma enzyme activity (P<0.05). Sodium selenite and selenium nanoparticles at levels of 0.3 and 0.6 ppm significantly decreased triglyceride, cholesterol and LDL in the plasma of birds (P<0.05). Conclusion 0.3 ppm of selenium nanoparticles and 0.6 ppm of Sodium selenitecan be successfully used to improve the antioxidant status and reduce plasma bad fats.
Elham Hoveizi
Abstract
Lung cancer is one the most common cancer in the world and Iran that lead to many deaths. Despite abundant researches, an appropriate cure has not been yet found for cancer. Citrus extract contains abundant anti-oxidative compounds. In this research evaluated and compared the toxic effect ...
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Lung cancer is one the most common cancer in the world and Iran that lead to many deaths. Despite abundant researches, an appropriate cure has not been yet found for cancer. Citrus extract contains abundant anti-oxidative compounds. In this research evaluated and compared the toxic effect of Citrus leaves hydroalcoholic extracts on the proliferation and viability of A459 cancer cells. Citrus leaves extracts were prepared by soxhlet set. A549 cells were proliferated and treated with 0.05-10 mg/ml concentrations of extracts for 72h. Cell viability and morphology were evaluated by MTT assay and acridine orange staining on certain times after treatment. Comparison of the cell viability percent in experimental groups indicated that IC50 were 2, 6, and 8 respectively for Citrus limon, Citrus reticulata, and Citrus aurantium leaves extract (P<0.05). Also, number of apoptotic cells was significantly (P<0.05) more in C.lemon group than C. reticulata and C. aurantium group and indeed, number of apoptotic cells were significantly (P<0.05) more in C. reticulata group than C. aurantium group. Citrus leaves hydroalcoholic extract can induce apoptosis in A549 cells in a dose and time-dependent manner.
Ameneh Jamshidi; Mohammad Ali Ebrahimi; Tayebeh Rajabian; Gholam Reza Bakhshikhaniki; Shahla Mozaffari
Abstract
Abstract Chlorella sorokiniana contains valuable metabolites such as proteins, antioxidants, lipids, vitamins and minerals, and is used as a food for animals and medicine. Therefore, in the present study, the effect of some culture factors and conditions on nutrient supplementation of algal levels for ...
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Abstract Chlorella sorokiniana contains valuable metabolites such as proteins, antioxidants, lipids, vitamins and minerals, and is used as a food for animals and medicine. Therefore, in the present study, the effect of some culture factors and conditions on nutrient supplementation of algal levels for aquaculture was investigated. For this purpose, algae were cultured in modified Bold Basal Media (BBM) and 0.5 gram of glucose was added as carbon source. Then, the effect of pH, addition of thiamine pyrophosphate, changes in nitrate and phosphate levels, addition of yeast and lack of glucose, were studied on growth and content of algal metabolites. The highest growth rate, antioxidant content, protein and some algal mineral content were obtained in medium containing twice the nitrate and phosphate with or without thiamine pyrophosphate. Adding yeast increased dry weight and decreased none significantly antioxidants. Adding thiamine pyrophosphate alone and low reduction of acidity did not significantly effect on growth and metabolites of alga. Lack of glucose significantly reduced alga growth.
mokhtar fathi; T. Tanha
Volume 1, Issue 1 , October 2012, , Pages 69-80
Abstract
160 one-day-old male broiler chicks (Ross308) in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group of these birds was rose in normal temperature (NT treatment) and the other group was raised in cold temperature induce PHS and heart failure ...
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160 one-day-old male broiler chicks (Ross308) in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group of these birds was rose in normal temperature (NT treatment) and the other group was raised in cold temperature induce PHS and heart failure (CT treatment). Glutathione peroxidase (GPX), superoxide dismutase SOD (, total antioxidant status (TAS) and malondialdehyde (MDA) content of both plasma and liver were determined at days 21 and 42 At the end of the experiment (day42), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. Results showed that MDA content in plasma and liver of CT birds was greater than that of NT birds at day 21 and 42. GPX activity in plasma and liver of CT birds at day 21&42 and SOD activity in plasma at day 42 were lower than that of NT birds. Furthermore, birds of NT treatment had a higher plasma TAS (P<0.05) at both ages. Moreover, RV/TV ratio at day 42 and mortality in total period were significantly higher in CT birds.
