Toxic Effects of Sodium ‎Polyacrylate at Different ‎Concentrations on Embryo ‎Development Rate and Expression ‎of Apoptotic and Antioxidant ‎Genes in Mouse Blastocysts

Khodabandeh, Zahra; Alaee, Sanaz; Dara, Mahintaj; Davari, Maryam; Bakhtari, Azizollah

doi:10.30473/eab.2023.61453.1852

Document Type : Article

Authors

¹ Assistant Professor, Stem Cells Technology Research Center, ‎Shiraz University of Medical Sciences, Shiraz, Iran

² Assistant Professor, Department of Reproductive Biology, ‎School of Advanced Medical Sciences and Technologies, ‎Shiraz University of Medical Sciences, Shiraz, Iran

³ Ph.D., Stem Cells Technology Research Center, Shiraz ‎University of Medical Sciences, Shiraz, Iran

https://doi.org/10.30473/eab.2023.61453.1852

Abstract

Sodium polyacrylate is a material with a high potential for water and moisture absorption. In this study, the effects of this material on the rate of embryo development and the expression of apoptosis-related and antioxidants genes in the blastocyst was evaluated. Adult female mice were superovulated by injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) and after placing beside adult male mice, zygotes were harvested from oviducts and transferred into media containing 0, 5, 25, and 50 μg/ml sodium polyacrylate. Zygotes were cultured towards the blastocyst stage and the rate of embryo development was assessed. Expression of intended genes were evaluated by real time RT-PCR. One-way analysis of variance (ANOVA) and Duncan's post hoc toast were used to determine the differences between the means of the groups. The rate of blastocysts was significantly lower in 50 μg/ml compared to the control group (P<0.05). The expression of Bcl-2 increased significantly in 5 μg/ml in comparison to the control group and decreased significantly in 50 μg/ml compared to 5 and 25 μg/ml (P<0.05). The expression of Bax/Bcl-2 and Caspase-3 in 50 μg/ml increased significantly in comparison to 0, 5, and 25 μg/ml (P<0.05). Therefore, the high concentration of sodium polyacrylate has an adverse effect on the embryo through apoptosis system.

Keywords

References

Cao, W.; Zhu, X.; Tang, Z.; Song, Y. (2019). A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica. JoVE (Journal of Visualized Experiments);(146): e58560.

Driscoll, K.E. (1996). Role of inflammation in the development of rat lung tumors in response to chronic particle exposure. Inhalation toxicology; 8: 139-154.

Esteves, S.C. (2014). Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. International braz j urol; 40(4): 433-453.

Garay-Jimenez, J.C.; Turos, E. (2011). A convenient method to prepare emulsified polyacrylate nanoparticles from for drug delivery applications. Bioorganic & medicinal chemistry letters; 21(15): 4589-4591.

Haselbach, J.; Berner, T.; Wright, H.; Dunlap, E. (2000). Single-Dose Oral Toxicity Study of a Cross-linked Sodium Polyacrylate/Polyvinyl Alcohol Copolymer in Chickens (Gallus domesticus). Regul. Toxicol. Pharmacol.; 32(3): 332-336.

Henderson, W.R.; Barnbrook, J.; Dominelli, P.B.; Griesdale, D.E.; Arndt, T.; Molgat-Seon, Y.; Foster, G.; Ackland, G.L.; Xu, J.; Ayas, N.T. (2014). Administration of intrapulmonary sodium polyacrylate to induce lung injury for the development of a porcine model of early acute respiratory distress syndrome. Intensive care medicine experimental; 2(1): 1-16.

Hong, S.H.; Ham, S.Y.; Kim, J.S.; Kim, I.-S.; Lee, E.Y. (2016). Application of sodium polyacrylate and plant growth-promoting bacterium, Micrococcaceae HW-2, on the growth of plants cultivated in the rooftop. international biodeterioration & biodegradation; 113: 297-303.

Khodadadi Dehkordi, D.; Shamsnia, S.A. (2020). Application of Reclaimed Sodium Polyacrylate to Increase Soil Water Retention. CLEAN–Soil, Air, Water; 48(11): 2.

Liu, Y.; Sun, Y.; Sun, L.; Wang, Y. (2016). In vitro and in vivo study of sodium polyacrylate grafted alginate as microcapsule matrix for live probiotic delivery. Journal of Functional Foods; 24: 429-437.

Manzur, T.; Iffat, S.; Noor, M.A. (2015). Efficiency of sodium polyacrylate to improve durability of concrete under adverse curing condition. Advances in Materials Science and Engineering; 2015.

Müller, G. (1987). Bekturov, EA; Bakauova, Z. Kh.: Synthetic Water‐Soluble Polymers in Solution 241 S., 87 Abb., 30. Tab., Format 15× 23 cm. Basel/Heidelberg/New York: Huethig & Wepf 1986, Wiley Online Library.

Ntekpe, M.E.; Mbong, E.O.; Edem, E.N.; Hussain, S. (2020). Disposable Diapers: Impact of Disposal Methods on Public Health and the Environment. Am J Med Public Health. 2020; 1 (2); 1009.

Pandey, S.P.; Shukla, T.; Dhote, V.K.; Mishra, D.K.; Maheshwari, R.; Tekade, R.K. (2019). Use of polymers in controlled release of active agents. Basic fundamentals of drug delivery, Elsevier: 113-172.

Petkar, K.C. (2019). Polyacrylate Nanoparticles as a Promising Tool for Anticancer Therapeutics. Polymeric Nanoparticles as a Promising Tool for Anti-cancer Therapeutics, Elsevier; 35-56.

Rai, P.; Lee, B.-M.; Liu, T.-Y.; Yuhui, Q.; Krause, E.; Marsman, D.S.; Felter, S. (2009). Safety evaluation of disposable baby diapers using principles of quantitative risk assessment. Journal of Toxicology and Environmental Health, Part A; 72(21-22): 1262-1271.

Reingold, A.L. (1991). Toxic shock syndrome: an update. American journal of obstetrics and gynecology; 165(4): 1236-1239.

Ren, H.; Huang, X. (2010). Polyacrylate nanoparticles: toxicity or new nanomedicine? European Respiratory Journal; 36(1): 218-221.

Ritthidej, G.C. (2011). Nasal delivery of peptides and proteins with chitosan and related mucoadhesive polymers. Peptide and protein delivery, Elsevier: 47-68.

Song, Y.; Li, X.; Du, X. (2009). Exposure to nanoparticles is related to pleural effusion, pulmonary fibrosis and granuloma. Eur. Respir. J.; 34(3): 559-567.

Soni, V.; Pandey, V.; Tiwari, R.; Asati, S.; Tekade, R.K. (2019). Design and evaluation of ophthalmic delivery formulations. Basic Fundamentals of Drug Delivery, Elsevier: 473-538.

Tiwari, R.R.; Sadhu, H.G.; Sharma, Y.K. (2021). Respiratory health of workers exposed to polyacrylate dust. Lung India; 38(3): 252.

Turos, E.; Reddy, G.S.K.; Greenhalgh, K.; Ramaraju, P.; Abeylath, S.C.; Jang, S.; Dickey, S.; Lim, D.V. (2007). Penicillin-bound polyacrylate nanoparticles: restoring the activity of β-lactam antibiotics against MRSA. Bioorg. Med. Chem. Lett.; 17(12): 3468-3472.

Turos, E.; Shim, J.-Y.; Wang, Y.; Greenhalgh, K.; Reddy, G.S.K.; Dickey, S.; Lim, D.V. (2007). Antibiotic-conjugated polyacrylate nanoparticles: new opportunities for development of anti-MRSA agents. Bioorg. Med. Chem. Lett.; 17(1): 53-56.

Zhu, X.; Cao, W.; Chang, B.; Zhang, L.; Qiao, P.; Li, X.; Si, L.; Niu, Y.; Song, Y. (2016). Polyacrylate/nanosilica causes pleural and pericardial effusion, and pulmonary fibrosis and granuloma in rats similar to those observed in exposed workers. International journal of nanomedicine; 11: 1593.

Zhuang, W.; Li, L.; Liu, C. (2013). Effects of sodium polyacrylate on water retention and infiltration capacity of a sandy soil. SpringerPlus, Springer.

Experimental animal Biology

Toxic Effects of Sodium ‎Polyacrylate at Different ‎Concentrations on Embryo ‎Development Rate and Expression ‎of Apoptotic and Antioxidant ‎Genes in Mouse Blastocysts

References

References

Volume 11, Issue 1 - Serial Number 41
No.1
June 2022
Pages 1-9

Toxic Effects of Sodium ‎Polyacrylate at Different ‎Concentrations on Embryo ‎Development Rate and Expression ‎of Apoptotic and Antioxidant ‎Genes in Mouse Blastocysts

References

References

Volume 11, Issue 1 - Serial Number 41 No.1June 2022Pages 1-9

Volume 11, Issue 1 - Serial Number 41
No.1
June 2022
Pages 1-9