Investigation of antioxidant and ‎Inhibitory properties of soybean ‎seeds extract (Glycine max) on the ‎acetylcholinesterase and the ‎production of Amyloid Nano-‎biofibrils

Fallah Digsara, Fereshteh; Arasteh, Amir

doi:10.30473/eab.2021.48416.1735

Document Type : Article

Authors

¹ M. A., Department of Biology, Rasht Branch, Islamic ‎Azad University, Rasht, Iran ‎

² Assistant Professor, Department of biology, Rasht ‎Branch, Islamic Azad University, Rasht, Iran

https://doi.org/10.30473/eab.2021.48416.1735

Abstract

Soybean (Glycine max) is a plant of the legume family and one of the most important oil plants. Soybean is rich in various proteins and phytochemicals, especially isoflavones. The aim of this study was to investigate the antioxidant and inhibitory effects of hydro-alcoholic extract of soybean seed on acetyl cholinesterase and production of amyloid nanobiofibrils in bovine serum albumin protein as a model protein. First, the yellow soybean seeds was powdered, then its hydro-alcoholic extract was prepared. The extracts composition were obtained by GC-MS spectroscopy. Anti-Alzheimer's effects were performed by measuring antioxidant activity by DPPH method, Inhibition of acetylcholinesterase by ellman method and inhibition of the production of amyloid nanobiofibrils by congored absorption method. Transmission electron microscopy was used to confirm the presence of amyloid fibrils. The presence of genistein active component in yellow soybean seed extract was confirmed by 2.97% and palmitic and linoleic acids by 3.11 and 10.69%, respectively. Investigation of antioxidant activity of soybean extract in different doses showed that with increasing the concentration of the extract, the percentage of antioxidant activity also increases. Investigation of acetylcholinesterase inhibition using a substrate analogue called acetylthiocholine iodide (ATCI) showed that with increasing the concentration of the extract, the amount of enzyme inhibition increases. The amounts of extract at high concentrations reduced the production of amyloid nanofibrils. This was confirmed by electron microscope images.Yellow soybeans, with their good antioxidant and inhibitory effects on the acetylcholinesterase enzyme, can be a good candidate for reducing the side effects of Alzheimer's.

Keywords

20.1001.1.23222387.1400.10.1.1.1

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Experimental animal Biology

Investigation of antioxidant and ‎Inhibitory properties of soybean ‎seeds extract (Glycine max) on the ‎acetylcholinesterase and the ‎production of Amyloid Nano-‎biofibrils

References

References

Volume 10, Issue 1 - Serial Number 37
No.1
August 2021
Pages 11-22

Investigation of antioxidant and ‎Inhibitory properties of soybean ‎seeds extract (Glycine max) on the ‎acetylcholinesterase and the ‎production of Amyloid Nano-‎biofibrils

References

References

Volume 10, Issue 1 - Serial Number 37 No.1August 2021Pages 11-22

Volume 10, Issue 1 - Serial Number 37
No.1
August 2021
Pages 11-22