In Vitro effect of different levels ‎of peppermint (Mentha piperital) ‎extract on semen quality ‎parameters in Arabi ram

Golandam, Shadan; Tabatabaei Vakili, Saleh; Mirzadeh, Khalil

doi:10.30473/eab.2021.56997.1811

Document Type : Article

Authors

¹ M. A., Department of Animal Science, Faculty of Animal and ‎Food Science, Agricultural Sciences and Natural Resources ‎University of Khuzestan, Ahvaz, Iran

² Associate Professor, Department of Animal Science, Faculty of ‎Animal and Food Science, Agricultural Sciences and Natural ‎Resources University of Khuzestan, Ahvaz, Iran

https://doi.org/10.30473/eab.2021.56997.1811

Abstract

The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint extract. At different storage times of diluted semen containing treatments (zero, 24, 48 and 72 hours) in liquid condition, semen quality parameters were evaluated. Immediately after sperm collection, the lowest total motility and sperm viability belonged to the level of 200 peppermint extract (P<0.05). At 24 hours, levels 150 and 200 of extract increased the rate of morphological abnormalities of sperms. At this time, the lowest level of sperm plasma membrane integrity was related to the level of 200 peppermint extracts (P<0.05). 48 hours after sperm storage, the highest total motility, viability and plasma membrane integrity of sperms were belonged to the control group (P<0.05). 72 hours after semen storage, no statistically significant differences were found among treatments for all sperm quality parameters. At this time, the highest concentration of malondialdehyde (MDA) in seminal plasma was related to the level of 100 μl/ml peppermint extract (P<0.05). In general, by adding different levels of peppermint extract to the diluent and maintaining the diluted semen of the Arabi ram in a liquid state at 5°C, the concentrations used of peppermint extract not only did not improve the qualitative parameters of the sperm, it also had a devastating effect.

Keywords

20.1001.1.23222387.1400.10.1.7.7

References

Agarwal, A.; Sekhon, L.H. (2010). The role of antioxidant therapy in the treatment of male infertility. Human Fertility; 13(4):217-225.

Akdogan, M.; Ozguner, M.; Kocak, A. (2004). Effects of peppermint teas on plasma testosterone, follicle- stimulating hormone and luteinizing hormone levels and testicular tissue in rats. Urology; 64:394-398.

Alizadeh, K.; Moslemipur, F.; Bahri Binabaj, F.; Mojtahedin, A. (2020). The effect of adding horsemint ethanol extract and cysteine into extender on fertility characteristic of frozen Baluchi ram semen after thawing. Journal of Ruminant Research; 8(1):49-62.

Amini Pour, H.; Tahmasebi, A.M.; Naserain, A.A. (2013). The influence of vitamin E on semen characteristics of ghezel rams in during cooling and frozen process. European Journal of Zoological Reserch; 2(5):94-99.

Angel, A.; Zamfirescu, S.; Coprean, D.; Sogorescu, E. (2009). The effects of cystein, bovine serum albumin and vitamin E on the calitative parameters of frozen-thawed ram semen. Annals of the Romanian Society for Cell Biology; 14(2):133-136.

Baumber, J.; Ball, B.A.; Gravance, CG.; Medina, V. (2000). The effect of reactive oxygen species on equine sperm moltility, viability, acrosomal integtity, mitochondrial membrane potential and membrane lipid peroxidation. Andrology; 2000; 21: 895-902.

Blokhina, O.; Virolainen, E.; Fagersted, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivaion stress: a review. Annals of Botany; 91(2):179-194.

Daghigh Kia, H.; Sadeghi Sadegh Abad, F.; Mohamadzadeh, H.; Vaseghi Dodran, H.; Ashrafi, I. (2016). The effect of Origanum vulgare extract as natural antioxidant on quality cryopreserved ram sperm. 26(4): 111-120.

Fanaei, H.; Azizi, Y.; Khayat, S. (2013). A review: Role of oxidative stress in male infertility. Journal of Fasa University of Medical Sciences; 3(2):93-103.

Farhadi, R.; Daghighkia, H.; Hosseinkhani, A.; Ghasemi Panahi B., Dehghan GH.; Ashrafi, I. (2015). Effect of Origanium vulgare ethanol extract on quality parameters and malondialdehyde concentration of cryopreserved Holstein bull sperm. Animal Science Research; 25(1):1-11.

Foster, S. (1996). Peppermint: Mentha piperita. American Botanical Council-Botanical Series; 36:3-8.

Ghazvinian Kh.; Javaheri Vayghan. A.; Irani A. Reproductive physiology and applied artificial insemination in sheep and goat. Semnan University press, 273.

Halliwel, B.; Gutteridge, J.M.C (1989). Lipid preoxidation : a radical chain reaction. Free radicals in biology and medicine; 2:188-218.

Honari, N.; Pouraboli I. (2019). The effect of hydroalcoholic extract of Thymus caramanicus on serum testosterone and testis antioxidant enzymes levels in streptozotocin induced diabetic rats: An experimental study. Journal of Rafsanjan University of Medical Sciences; 17(11): 1017-1030.

Jalali-Kohi Kheili, S.M.; Mohammadi, M.; Roostaei-Ali Mehr, M. (2016). Effect of olive leaf extract on rooster semen storage. Journal of Animal Production. 18(2): 377-385.

Kumar, V.; Kural, M.R.; Pereira, .M.; Roy, P. (2008). Spearmint induced hypothalamic oxidative stress and testicular antiandrogenicity in male rats - altered levels of gene expression, enzymes and hormones. Food Chemistry and Toxicology. 46: 3563-3570.

Kursat, M.; Emre, I.; Yilmaz, O.; Erecevit, P. (2011). Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) ietswaart and origanum acutidens (Hand-Mazz) ietswaart from turket. Grasas Y Aceites; 62(4):410-417.

Memon, A.A.; Wahid, H.; Rosnina, Y.; Goh, Y.M.; Ebrahimi, M.; Nadia, F.M. (2012). Effect of antioxidants on post thaw microscopic, oxidative stress parameter and fertility of Boer goat spermatozoa in Tris egg yolk glycerol extender. Animal Reproduction Science; 136(1): 55-60.

McKay, D.L.; Blumberrg, J.B. (2006). A review of the bioactivity and potential health benefits of peppermint tea (mentha piperital Linn). Phytotherapy Research; 20(8):619-633.

Nozhat, F.; Alaee S.; Behzadi, Kh.; Azadi Chegini, N. (2014). Evaluation the possible toxic effects of spearmint (Mentha spicata) on the reproductive system, fertility and number of offspring of adult male rats. Avicenna Journal of Phytomedicine; 4(6):420-429.

Placer, Z.A.; Cushman, L.L.; Johnson, B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analitical Biochemistry; 16: 359-364.

Plante, M.; de Lamirande, E.; Gagnon, C. (1994). Reactive oxygen species released by activated neutrophils, but not by deficient spermatozoa, are sufficient to affect normal sperm motility. Fertility and Sterility; 62(2): 387-393.

Ramezaninejad, H.; Roostaei Ali Mehr, M. (2017). Storage of rooster semen in liquid form using alcoholic extract of rosemary. Iranian Journal of Animal Science; 48(1): 51-59.

Roca, J.; Gil, M.A.; Parrilla, I.; Vazquez, J.M.; Marinez, E.A. (2004). Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. Journal of Andrology; 25:397-405.

Shafigh, H.; Shakeri, M.; Zeinoldini, S.; Kohram, H.; ZHhandi, M.; Moghbeli, M. (2016). Improving sperm cryopreservation of rooster by using rosemary alcoholic essential oil. Journal of Animal Production; 18(3):615-624.

Sharma, N.; Jacob, D. (1996). Fertility suppression of the male mouse
after administration of mint leaf extract. Phytochemical Research; 10:175-177.

Tvrda, E.; Konecna, N.; Zbynovska, K.; Lukac, N. (2018). Antioxidant effects of Peppermint (Mentha piperita) extract on the oxidative balance of rabbit spermatozoa. Journal of Advanced Agricultural Technologies; 5(2):117-122.

Vahedi, V.; Gharibkhaje V.; Hedayat, N. (2018). Antioxidant effects of peppermint extract on the survival rate of frozen-thawed sperm of Moghani ram. The first national conference on novel ideas in agriculture and natural recourses.

Zanganeh, Z.; Zhandi, M.; Zare-Shahneh, A.; Najafi, A.; Nabi, M.; Mohammadisangcheshme, A. (2013). Does rosemary aqueus extract improve buck semen cryopreservation? Small Ruminant Reserch, 114:120-125.

Experimental animal Biology

In Vitro effect of different levels ‎of peppermint (Mentha piperital) ‎extract on semen quality ‎parameters in Arabi ram

References

References

Volume 10, Issue 1 - Serial Number 37
No.1
August 2021
Pages 71-79

In Vitro effect of different levels ‎of peppermint (Mentha piperital) ‎extract on semen quality ‎parameters in Arabi ram

References

References

Volume 10, Issue 1 - Serial Number 37 No.1August 2021Pages 71-79

Volume 10, Issue 1 - Serial Number 37
No.1
August 2021
Pages 71-79