T. Ghanbari; R. Abdi; M. Bahmani; R. Kazemi; A. Savari
Volume 3, Issue 4 , July 2015, Pages 1-8
Abstract
Abstract In this study, cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histology examination were done and paraffin blocks cut at 6 microne and then stained ...
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Abstract In this study, cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histology examination were done and paraffin blocks cut at 6 microne and then stained with H&E, Safranin-O and Verhoff staining. In photomicrographs were compared cell structure and compounds of substance grounds in cartilage tissues. It showed that perichondrium layer of dorsal fin was more advanced from perichondrium layer of vertebral column and their componants were different. Microscopic masurement of two cartilage tissue showed the cells of dorsal fin were larger than the cells of vertebral column but in cells number,decrised with physiological maturation. Comparative study of volume of extracellular matrix and number of isogenous groups by SPSS software showed that growth of cartilage tissue of vertebral column was interstitial but in dorsal fin was appositional.
H. Ghafari Farsani; S. A. Akbar Hedayati; M. Mansouri Chorehi; N. Rostamian; E. Chaharde Baladehi
Volume 3, Issue 4 , July 2015, Pages 9-16
Abstract
Abstract The aim of current study was to evaluate the effects of heavy metal cadmium on liver tissue of gold fish during experimental condition. Study was in a week on 5 treatments and 3 replicates, five concentration (2.8, 5.6, 8.4 and 11.2 ppm respectively) of cadmium as 0 (control), 25%, 50%, 75% ...
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Abstract The aim of current study was to evaluate the effects of heavy metal cadmium on liver tissue of gold fish during experimental condition. Study was in a week on 5 treatments and 3 replicates, five concentration (2.8, 5.6, 8.4 and 11.2 ppm respectively) of cadmium as 0 (control), 25%, 50%, 75% and 100% of LC50 were selected and tested. Histopathological changes were done using classic histology method in hematoxylin and eosin staining and using a light microscope and camera images properly installed and connected the camera to the computer system software was developed. The overall result of this study showed that in liver tissue exposed to cadmium over a week with increasing duration of exposure to pesticide exposure, a high level of toxin in the lesion by the most frequent lesions end of a week, was observed including necrosis, atrophy and cloudy swelling of the cell. Result of this study show that liver tissue have many lesions in response to cadmium in one week and it will be increase with concentration and duration of toxin, as it was seen highest amount of lesions at the end of experiment within last dose.
M. Jamalan; M. Amin; M. K. Safdari; N. Aghel
Volume 3, Issue 4 , July 2015, Pages 17-23
Abstract
Abstract Bacterial resistance to antibiotics is main challenge in the treatment of infectious diseases. Thus, investigation for alternative drugs is an essential phenomenon. This study designed to aim to evaluate the antimicrobial property of essential oil of Boswellia carterii against bacterial and ...
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Abstract Bacterial resistance to antibiotics is main challenge in the treatment of infectious diseases. Thus, investigation for alternative drugs is an essential phenomenon. This study designed to aim to evaluate the antimicrobial property of essential oil of Boswellia carterii against bacterial and fungal causative agents of the mouth, gastrointestinal tract infections and antibiotic-resistant species. Essential oils were isolated through microdilution tube using Clevenger's method. The antimicrobial activities of the essential oils were individually evaluated against different microorganisms including pathogenic fungus (candida albican and three type of aspergillus), Gram-positive (Streptococcus salivarius, Streptococcus sanguis and Lactobacillus) and Gram-negative (Salmonella, Pseudomonas, Shigella and E.coli) bacteria strains. The essential oils have activity against all eleven microbes and the best activity determined against Streptococcus (5.3 μg/ml) were obtained. On the other hand, pseudomonas aeruginosa showed the most resistant among bacterial and fungal agent (85 μg/ml). Antimicrobial effects of B. carterii on resistance pathogens such as Pseudomonas aeroginosa and candida albicans revealed the necessity of performing expanded research about composition and property of this plant.
A. Shakouri; O. Javanmard Kamy Ghzy Mahalleh
Volume 3, Issue 4 , July 2015, Pages 25-35
Abstract
Abstract
Echinoderms, ancient group of marine invertebrates, are a rich source of antibacterial compounds with high activity mechanism. In the present study, the antibacterial effect of sea urchin skin, Echinometra mathaei, was examined in different doses on some pathogenic bacterial strains. After ...
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Abstract
Echinoderms, ancient group of marine invertebrates, are a rich source of antibacterial compounds with high activity mechanism. In the present study, the antibacterial effect of sea urchin skin, Echinometra mathaei, was examined in different doses on some pathogenic bacterial strains. After sampling and transport of samples to the laboratory, animals were dissected, and the shucks separated after grinding it and, potentially bioactive metabolites were extracted using chloroform. The extracts were tested for activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Klebsiella pneumonia with varying concentration ranged from 1.25 to 11.25 mg/ml. The tests were performed by disk diffusion method. The results showed that the antibacterial effect of different concentrations of extracts on types of bacteria are significantly different (p
E. Haghighy; M. Sattari; S. Dorafshan; Y. Keivany
Volume 3, Issue 4 , July 2015, Pages 37-46
Abstract
Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters ...
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Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters were measured and thirteen meristictraits were counted on each specimen. Nonparametric methods for comparison of means and Principal Component Analysis (PCA) for separation of populations were used. Among the meristic characters, nine characters were significantly different between the four populations (p<0.05). Among the morphometric and relative morphometric characters, nineteen characters showed significant differences (p<0.05) between the two populations. In the PCA of meristic characters, three factors accounted for 47.5% of variations within populations, including; caudal peduncle scales, scale above lateral line and anal fin ray numbers. Within the morphometric characters, three factors accounted for about 71% of variations within the two populations, including dorsal fin height, pectoral fin base, ventral fin length, caudal peduncle depth, head length, inter orbital width, standard length to pre dorsal length, head length to snout length, head length to eye diameter and head length to inter orbital width. The results showed while morphometric and relative morphometric characters were not useful for separating the two populations and sexes, the meristic characters could relatively separate these two populations.
I. Gowhari; S. Molaei Birgani; Sh. Pashaei Rad; Sh. Navidpour
Volume 3, Issue 4 , July 2015, Pages 47-52
Abstract
Abstract Scorpions are the oldest terrestrial arthropodas. Scorpions dig their nest in the ground to protect themselves and their infants from light, high and low tempreture. Regarding habitat, species, depth and the palce of digging, the nest of scorpions would be various , with due regard to the climate ...
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Abstract Scorpions are the oldest terrestrial arthropodas. Scorpions dig their nest in the ground to protect themselves and their infants from light, high and low tempreture. Regarding habitat, species, depth and the palce of digging, the nest of scorpions would be various , with due regard to the climate of Khuzestan Province and the variety of Scorpion species in this Province, investigation of Scorpion nesting behaviors is an essential issue in Scorpionology. In this research, the nesting behaviors of two types of Scorpions Odothouths bidentatus and Scorpio mauruss were examined. In this Survey, length and width of the nest entrance were measured and small models of the nests were made by means of injecting materials such as cement and chalk in these nests. After the computation of mean, Standard deviation and Standard error of measurment applying T-test and the Significance of error less than%5 , the result of measuring width and length of the entrance, different parts of the nest including initial, middle, final and length of the nest of Scorpion s under investigation were compared. To compare the parameters insides the species, nonparametric Freedman method was applied, and also to compare the measured parameters concerning physicochemical properties of Scorpion soilbed, Mann Whitney method was used.
M. Larijani; S. H. Khabbazi Khadar; M. J. Yousefnezhad; Z. Rahimi Kalate
Volume 3, Issue 4 , July 2015, Pages 53-65
Abstract
Abstract
Fish meat has high nutritional value and putrescence. For this reason, it is very important increase the shelf life and fish meat quality. In this study tried to go up the shelf life and quality fish meat by ozon ice. The number of Rainbow trout with weight 300±10 purchased and ...
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Abstract
Fish meat has high nutritional value and putrescence. For this reason, it is very important increase the shelf life and fish meat quality. In this study tried to go up the shelf life and quality fish meat by ozon ice. The number of Rainbow trout with weight 300±10 purchased and transferred to the Mashhad laboratory (Iran) immediately. The fish was divided into four groups, control (A), dose of 3g (B) 4g (C) and 5g of ozone per 50 kg ice with the brine 5% (D). Daily to the fourteenth day of corruption were used on the WHO chart. Moreover sampling of skin, meat and gill tissue was done every day to the 12th day for check bacterial by (PCA) cultured. The amount of histamine (ELISA method), peroxide (PV) (Egan method) and TVN (Kldal distiller method), was obtained respectively. The results showed that A sample (control) had corrupted the most number of bacteria in depth and surface have existence ten days significantly (P <0.05). Amounts of peroxide and TVN in B sample lower than to the other fish samples and had no significant increased (P 0.05). Histamine changes was not significant in all samples (P<0.05) and all of the fishes ozone treated have better maintenace and quality. Thus it can be infered that ozone is an effective mater to increase the durability of fish.
M. Bayati; R. Patimar; M. Farhangi; M. Farhangi; M. Forouhar Vajargah
Volume 3, Issue 4 , July 2015, Pages 67-78
Abstract
Abstrac The aim of this study was to determinate the effect of trout farm sewage on fecundity and diversity of macro invertebrate benthoses with some biological indices. This study was done on the Marber River in Semirom city, the southwest area of Isfahan province. Sampling was took place in five stations ...
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Abstrac The aim of this study was to determinate the effect of trout farm sewage on fecundity and diversity of macro invertebrate benthoses with some biological indices. This study was done on the Marber River in Semirom city, the southwest area of Isfahan province. Sampling was took place in five stations on summer of 2012, monthly using a Surber sampler (30.5cm×30.5 cm). Sampling in each station was done in three points (edges and middle of the river). Captured samples were fixed in 4% formalin and were taken to the laboratory. In laboratory animals were collected with a sieve of 500 microns in diameter springs to assort and identify to the least level possible (families and genera). In this study, several biological parameters such as: Abundance, Biomass, Similarity, Shannon-Wiener, EPT/ Chironomidae, Margalef richness, Hylsnhvf, BMWP index and ASPT index was used. Eventually 20 families, with 10 orders, 7 classes and 5 phyla were identified.
P. Khodarahmi; H. Nasrollahzade Phili; M. Heydari Nasrabadi
Volume 3, Issue 4 , July 2015, Pages 79-87
Abstract
Abstract
Silver nanoparticles have numerous applications in food technology and medical industry. These applications are mediated via their mitotic, oxidative and apoptotic effects. In this study, the apoptotic effects of short and long term administrations of nanosilver on rat hippocampus, which is ...
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Abstract
Silver nanoparticles have numerous applications in food technology and medical industry. These applications are mediated via their mitotic, oxidative and apoptotic effects. In this study, the apoptotic effects of short and long term administrations of nanosilver on rat hippocampus, which is involved in memory and learning, was investigated. 56 male Wistar rats were divided into two groups of short term and long term injections which were treated for 5 and 10 consecutive days, respectively. Each group was divided into four subgroups of control, 100, 200 and 400 ppm nanosilver administration. The control subgroup received saline and the treatment subgroups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal tissue sections were prepared and the extent of apoptosis was evaluated using TUNEL assay. In short term (5-day injection) group, the percentage of apoptotic cells between control and 100 (p
