نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه زیست دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران
2 گروه علوم جانوری و زیست‌شناسی دریا، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران
10.30473/eab.2025.74307.1991
چکیده
کیتین در اسکلت خارجی بسیاری از بی‌مهرگان، دیواره سلولی اکثر قارچ‌ها و برخی از جلبک‌ها یافت می‌شود که بر اثر واکنش‌هایی به کیتوزان تبدیل می‌شود. کیتوزان هم پلیمری ترکیبی گلوکز آمین و N استیل گلوکز آمین است که به‌وسیله پیوندهای ۱و ۴ گلیکوزیدی به هم متصلند. در این پژوهش ویژگی‌های ساختاری و فیزیکوشیمیایی کیتوزان استخراجی از ضایعات پوسته میگو گونه Penaeus merguiensis و صدف ماهی مرکب Sepia pharaonis مقایسه شده است. نمونه‌ها از ساحل بندرعباس جمع‌آوری شد و به‌منظور استحصال کیتین و کیتوزان، مشخصات فیزیکوشیمیایی با روش‌های FTIR، XRD و SEM مورد سنجش قرار گرفت. وزن مولکولی نمونه‌های کیتوزان به‌دست‌آمده از پوسته میگو موزی و صدف ماهی‌مرکب به‌ترتیب معادل 26000 و 14000 کیلودالتون مورد محاسبه قرار گرفت و درصد استیل‌زدایی کیتوزان در گونه P. merguiensis و S. pharaonis به‌ترتیب 66 و 5/30 درصد محاسبه شد. یافته‌ها نشان داد که میکروگراف‌های پوسته میگو ناهمگن و غیر‌مسطح و به‌صورت ساختارهای میله‌ای شکل شکسته و مورفولوژی سطح کیتوزان تهیه‌شده از صدف ماهی مرکب سطحی صاف را با تعداد کمی ساختار بزرگ‌تر و سنگ مانند روی آن مشاهده شد. در طی سال‌های اخیر افزایش توجه مجامع علمی به محصولات کیتوزانی حاصل از ضایعات دریایی برای این بیوپلیمر افزایش یافته و این بررسی بیانگر این امر بود که با توجه حجم زیاد میزان ضایعات دریایی تولید شده در بندرعباس و با درنظرگرفتن ملاحظات زیست محیطی نسبت به استخراج مشتقات کیتوزانی از ضایعات دریایی اقدام نمود.
کلیدواژه‌ها
موضوعات
کشاورز، موسی (1399). گونه‌های سپیاهای اقیانوسی. انتشارات دانشگاه هرمزگان، 117 صفحه.
موسوی‌نسب، مرضیه؛ موسوی‌نسب، سحرالسادات؛ مصباحی، غلامرضا و جمالیان، جلال (1393). ارزیابی خصوصیات کیفی کیتوزان تولیدی از پوسته میگو و کیتوزان تجاری پوسته خرچنگ. فصلنامه علوم و صنایع غذایی. 45 (11)، 174-163.
Abdou, E. S., Nagy, K. S., & Elsabee, M. Z. (2008). Extraction and characterization of chitin and chitosan from local sources. Bioresource technology, 99(5), 1359-1367.
Abdulkarim, A., Isa, M. T., Abdulsalam, S., Muhammad, A. J., & Ameh, A. O. (2013). Extraction and characterisation of chitin and chitosan from mussel shell. Civil and Environmental Research, 3(2), 108-114.
Ahing, F. A., & Wid, N. (2016). Optimization of shrimp shell waste deacetylation for chitosan production. International Journal of advanced and applied sciences, 3(10), 31-36.
Al Sagheer, F. A., Al-Sughayer, M. A., Muslim, S., & Elsabee, M. Z. (2009). Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbohydrate polymers, 77(2), 410-419.
Alterary, S. S., & Marei, N. H. (2022). New isolated shrimp (Litopenaeus setiferus) chitosan-based films loaded with fly ash for antibacterial evaluation. Polymers, 14(10), 2099.
AOAC, (2007). Official Methods of Analysis of the Association of Official Analytical Chemistry (A.O.A.C.) International, 18th Ed., Gaithersburg, Maryland, USA
Arafat, A., Samad, S. A., Masum, S. M., & Moniruzzaman, M. (2015). Preparation and characterization of chitosan from shrimp shell waste. International journal of scientific & engineering research, 6(5), 538-541.
Boudouaia, N., Bengharez, Z., & Jellali, S. (2019). Preparation and characterization of chitosan extracted from shrimp shells waste and chitosan film: application for Eriochrome black T removal from aqueous solutions. Applied Water Science, 9(4), 91.
Cho, Y.I., No, H. K., & Meyers, S. P. (1998). Physicochemical characteristics and functional properties of various commercial chitin and chitosan products. Journal of Agricultural Food Chemistry, 46:3839-3843.
Choi, C. R., Kim, E. K., Kim, Y. S., Je, J. Y., An, S. H., Lee, J. D., & Park, P. J. (2012). Chit oligosaccharides decreases plasma lipid levels in healthy men. International Journal of Food Sciences and Nutrition, 63(1), 103-106.
Gartner, C., Peláez, C. A., & López, B. L. (2010). Characterization of chitin and chitosan extracted from shrimp shells by two methods. e-Polymers, 10(1), 069.
Ghormade, V., Pathan, E.K., & Deshpande, M.V. (2017). Can fungi compete with marine sources for chitosan production?, International Journal of Biological Macromolecules, 104, 1415-1421.
Hongpattarakere, T., & Riyaphan, O. (2008). Effect of deacetylation conditions on antimicrobial activity of chitosans prepared from carapace of black tiger shrimp (Penaeus monodon). Songklanakarin Journal of Science & Technology, 30(Suppl. 1), 1-9.
Hussain, I., Singh, T., & Chittenden, C. (2012). Preparation of chitosan oligomers and characterization: Their antifungal activities and decay resistance. NZ Forest Research Institute, 66: 119-125.
Isa, M.T., Ameh, A. J., Gabreil, J. O., & Adama, K. K. (2012). Extraction and characterization of chitin from Nigeria sources. Leonardo Electronic Journal of Practices and Technologies, 21, 73-81.
Jan, S. S., Liu, D. C., Dong, X. Y., Hu, Y. M., & Chen, J. D. (2012). Effects of chitosan and its derivative added to water on immunological enhancement and disease control. Immunotherapy, 4(7), 697-701.
Kasaai, M. R. (2007). Calculation of Mark-Houwink-Sakurada (MHS) equation viscometric constants for chitosan in any solvent-temperature system using experimental reported viscometric constants data. Carbohydrate Polymers, 68(3), 477-488.
Keshavarz, M. (2020). Species of oceanic sepien. 1st Ed., University of Hormozgan. Bandar Abbas. 117 pp. (In Persian)
Khoerunnisa, F., Yolanda, Y. D., Nurhayati, M., Zahra, F., Nasir, M., Opaprakasit, P., Choo, M. Y., & Ng, E. P. (2021). Ultrasonic Synthesis of Nanochitosan and Its Size Effects on Turbidity Removal and Dealkalization in Wastewater Treatment. Inventions, 6(4), 98.
Kucukgulmez, A., Celik, M., Yanar, Y., Sen, D., Polat, H., & Kadak, A.E. (2011). Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells. Food Chemistry, 126(3), 1144-1148.
Kumari, S., & Rath, P. K. (2014). Extraction and characterization of chitin and chitosan from (Labeo rohit) fish scales. Procedia Materials Science, 6, 482-489.
Kumirska, J., Weinhold, M. X., Thöming, J., & Stepnowski, P. (2011). Biomedical activity of chitin/chitosan based materials-Influence of physicochemical properties apart from molecular weight and degree of N-acetylation. Polymers. 3, 1875-1901.
Mousevi nasab, M., Mousevi nasab, S. S., Mesbahi, G. H., & Jamalian, J. (2014). Evaluation of the quality characteristics of chitosan produced from shrimp shell and commercial chitosan from crab shell. Journal of Food Science and Technology., 45(11), 163-174. (In Persian)
Nessa, F., Masum, S. M., Asaduzzaman, M., Roy, S. K., Hossain, M. M., & Jahan, M. S. (2010). A process for the preparation of chitin and chitosan from prawn shell waste. Bangladesh Journal of Scientific and Industrial Research, 45(4), 323-330.
No, H. K., & Prinyawiwatkul, W. (2009). Stability of chitosan powder during long-term storage at room temperature. Journal of Agricultural and Food Chemistry, 57(18), 8434-8438.
No, H. K., Cho, Y. I., Kim, H. R., & Meyers, S. P. (2000). Effective deacetylation of chitin under conditions of 15 psi/121oC. Journal of Agricultural Food Chemistry, 48(6), 2625-2627.
Nouri, M., Khodaiyan, F., Razavi, H. S., & Mousavi, M. (2016). Improvement of chitosan production from Persian Gulf shrimp waste by response surface methodology. Food Hydrocolloids, 59, 50-58.
Omar, B. A., Elmasry, R., Eita, A., Soliman, M. M., El-Tahan, A.M., & Sitohy, M. (2022). Upgrading the preparation of high-quality chitosan from Procambarus clarkii wastes over the traditional isolation of shrimp chitosan. Saudi Journal of Biological Sciences, 29(2), 911-919.
Pal, J., Verma, H. O., Munka, V. K., Maurya, S. K., Roy, D., & Kumar, J. (2014). Biological method of chitin extraction from shrimp waste an eco-friendly low cost technology and its advanced application. International Journal of Fisheries and Aquatic Studies, 1(6), 104-107.
Pangestuti, R., & Kim, S.K. (2010). Neuroprotective properties of chitosan and its derivatives. Marine Drugs, 8(7), 2117-212.
Park, S. C., Nam, J. P., Kim, J. H., Kim, Y. M., Nah, J. W., & Jang, M. K. (2015). Antimicrobial action of water-soluble β-chitosan against clinical multi-drug resistant bacteria. International journal of molecular sciences. 16(4), 7995-8007.
Parthiban, F., Balasundari, S., Gopalakannan, A., Rathnakumar, K., & Felix, S. (2017). Comparison of the quality of chitin and chitosan from shrimp, crab and squilla waste. Current World Environment, 12(3), 672-679.
Peniche, C., Argüelles-Monal, W., & Goycoolea, F. M. (2008). Chitin and Chitosan: Major Sources, Properties and Applications. In: Monomers, Polymers and Composites from Renewable Resources, Elsevier, 517-542.
Puvvada, Y. S., Vankayalapati, S., & Sukhavasi, S. (2012). Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. International Current Pharmaceutical Journal, 1(9), 258-263.
Renuka, V., Ravishankar, C. N. R., Elavarasan, K., Zynudheen, A. A., & Joseph, T. C. (2019). Production and characterization of chitosan from shrimp shell waste of Parapeneopsis stylifera. International Journal of Current Microbiology and Applied Sciences, 8(11), 2076-2083.
Rinaudo, M. (2006). Chitin and chitosan: Properties and applications. Properties and applications, 31(7), 603-632.
Salman, D. D., Ulaiwi, W. S., & Qais, A. (2018). Preparation of chitosan from Iraqi shrimp shell by autoclave, studying some physiochemical properties and antioxidant activity. Journal of Pharmaceutical Sciences and Research, 10(12), 3120-3123.
Sowjanya, N. T., Dhivya, R., Meenakshi, K., & Vedhanayakisri, K. A. (2013). Potential applications of chitosan nanoparticles as novel support in enzyme immobilization. Research Journal of Engineering and Technology, 4(4), 14.
Sreelekshmi, RS., Alex, L., & Jose, J. J. (2022). Shelf-Life Specific Moisture Variation in Chitosan of Genus Fenneropenaeus Distributed Along Arabian Sea, India. International Journal of Innovative Research in Technology, 9(2), 247-253.
Szymańska, E., & Winnicka, K. (2015). Stability of chitosan-a challenge for pharmaceutical and biomedical applications. Marine drugs, 13(4), 1819-1846.
Toan, N. V. (2009). Production of chitin and chitosan from partially autolyzed shrimp shell materials. The open biomaterials journal, 1(1), 21-24.
Tsaih, M. L., & Chen, R. H. (2003). The effect of reaction time and temperature during heterogenous alkali deacetylation on degree of deacetylation and molecular weight of resulting chitosan. Journal of applied polymer science, 88(13), 2917-2923.
Vázquez, R., Caro-León, F. J., Nakal, A., Ruiz, S., Doñoro, C., García-Fernández, L., Vázquez-Lasa, B., San Román, J., Sanz, J., García, P., & Aguilar, M. R. (2021). DEAE-chitosan nanoparticles as a pneumococcus-biomimetic material for the development of antipneumococcal therapeutics. Carbohydrate Polymers, 273, 118605.
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Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., & Liu, W. (2012). Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses. Molecular Medicine Reports, 6(2), 385-390.
Xu, Y., Liang, K., Ullah, W., Ji, Y., & Ma, J. (2018). Chitin nanocrystal enhanced wet adhesion performance of mussel-inspired citrate-based soft-tissue adhesive. Carbohydrate polymers, 190, 324-330.
Yang, E. J., Kim, J. G., Kim, J. Y., Kim, S. C., Lee, N. H., & Hyun, C. G. (2010). Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells. Central European Journal of Biology, 5, 95-102.
Yen, M. T., Yang, J. H., & Mau, J. L. (2009). Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate polymers, 75(1), 15-21.
Younes, I., & Rinaudo, M. (2015). Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine drugs, 13(3), 1133-1174.
Zargar, V., Asghari, M., & Dashti, A. (2015). A review on chitin and chitosan polymers: structure, chemistry, solubility, derivatives, and applications. ChemBioEng reviews, 2(3), 204-226.
Zhou, H. Y., Chen, X. G., Kong, M., Liu, C. S., Cha, D. S., & Kennedy, J. F. (2008). Effect of molecular weight and degree of chitosan deacetylation on the preparation and characteristics of chitosan thermosensitive hydrogel as a delivery system. Carbohydrate polymers, 73(2), 265-273.
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