نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه فیزیولوژی ورزشی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران
2 گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران
10.30473/eab.2025.71559.1956
چکیده
مطالعه حاضر با هدف بررسی اثر تمرینات تناوبی و تداومی بر بیان گیرنده لپتین در بافت مغز و میزان کالری مصرفی در موش‌های صحرایی دیابتی سالمند انجام شد. این پژوهش بنیادی و تجربی روی 32 موش ماده نژاد اسپراگ داولی انجام شد. موش‌ها به‌طور تصادفی به چهار گروه تقسیم شدند؛ کنترل سالم، کنترل دیابتی، دیابتی تمرین تناوبی و دیابتی تمرین تداومی (هر گروه هشت نمونه). پس از اعمال پروتکل‌های تمرینی در گروه‌های مربوطه، سطح لپتین و کالری مصرفی ارزیابی شدند. بیان ژن لپتین با استفاده از روشReal- Time PCR و کالری مصرفی در هفته اول و آخر اندازه‌گیری شد. نتایج نشان داد که تمرین تناوبی تأثیر معنی‌داری بر بیان ژن لپتین در بافت مغز موش‌های صحرایی دیابتی سالمند داشت (001/0=P). علاوه بر این، پس از هشت هفته تمرین، تفاوت معنی‌داری در میزان کالری مصرفی بین گروه‌ها مشاهده شد (001/0=P). آزمون توکی نیز نشان داد که تفاوت معنی‌داری بین گروه تمرین تناوبی شدید و تداومی از نظر بیان لپتین وجود دارد (03/0=P). همچنین، کالری مصرفی در گروه کنترل دیابتی نسبت به دو گروه تمرین افزایش یافت (001/0=P). تأثیر تمرینات تناوبی و تداومی بر کالری مصرفی یکسان بود (58/0=P). تمرینات تناوبی به‌طور مؤثرتری از تمرینات تداومی بر بیان ژن لپتین در بافت مغز موش‌های صحرایی دیابتی سالمند تأثیر می‌گذارند. با این‌حال، هر دو نوع تمرین بر میزان کالری مصرفی تأثیر یکسانی داشتند.
کلیدواژه‌ها
موضوعات
Abbenhardt, C., McTiernan, A., Alfano, C. M., Wener, M. H., Campbell, K. L., Duggan, C., Foster‐Schubert, K. E., Kong, A., Toriola, A. T., & Potter, J. D. (2013). Effects of individual and combined dietary weight loss and exercise interventions in postmenopausal women on adiponectin and leptin levels. Journal of internal medicine, 274(2), 163-175.
Afzalpour, M. E., Chadorneshin, H. T., Foadoddini, M., & Eivari, H. A. (2015). Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiology & Behavior, 147, 78-83. https://doi.org/https://doi.org/10.1016/j.physbeh.2015.04.012
Alkhatib, A., Tsang, C., Tiss, A., Bahorun, T., Arefanian, H., Barake, R., Khadir, A., & Tuomilehto, J. (2017). Functional foods and lifestyle approaches for diabetes prevention and management. Nutrients, 9(12), 1310.
Aparicio, V., Coll-Risco, I., Camiletti-Moirón, D., Nebot, E., Martínez, R., López-Jurado, M., & Aranda, P. (2016). Interval aerobic training combined with strength-endurance exercise improves metabolic markers beyond caloric restriction in Zucker rats. Nutrition, Metabolism and Cardiovascular Diseases, 26(8), 713-721.
Becic, T., Studenik, C., & Hoffmann, G. (2018). Exercise increases adiponectin and reduces leptin levels in prediabetic and diabetic individuals: systematic review and meta-analysis of randomized controlled trials. Medical sciences, 6(4), 97.
Cavalcante, L. P., da Rosa Lima, T., de Almeida, P. C., Tolazzi, G. J., Ávila, E. T. P., Navalta, J. W., Junior, R. C. V., & Voltarelli, F. A. (2021). Intermittent fasting compromises the performance of eutrophic rats submitted to resistance training. Nutrition, 86, 111187.
Cho, N. H., Shaw, J., Karuranga, S., Huang, Y., da Rocha Fernandes, J., Ohlrogge, A., & Malanda, B. (2018). IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes research and clinical practice, 138, 271-281.
Colberg, S.R., Sigal, R.J., Yardley, J. E., Riddell, M.C., Dunstan, D.W., Dempsey, P.C., Horton, E.S., Castorino, K., & Tate, D.F. (2016). Physical activity/exercise and diabetes: a position statement of the American Diabetes Association. Diabetes care, 39(11), 2065.
Dinari Ghozhdi, H., Heidarianpour, A., Keshvari, M., & Tavassoli, H. (2021). Exercise training and de-training effects on serum leptin and TNF-α in high fat induced diabetic rats. Diabetology & Metabolic Syndrome, 13(1), 57.
Dundar, A., Kocahan, S., & Sahin, L. (2021). Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training. Archives of physiology and biochemistry, 127(4), 291-295.
Fatouros, I., Tournis, S., Leontsini, D., Jamurtas, A., Sxina, M., Thomakos, P., Manousaki, M., Douroudos, I., Taxildaris, K., & Mitrakou, A. (2005). Leptin and adiponectin responses in overweight inactive elderly following resistance training and detraining are intensity related. The Journal of Clinical Endocrinology & Metabolism, 90(11), 5970-5977.
Hopkins, M., Gibbons, C., Caudwell, P., Webb, D.-L., Hellström, P. M., Näslund, E., Blundell, J. E., & Finlayson, G. (2014). Fasting leptin is a metabolic determinant of food reward in overweight and obese individuals during chronic aerobic exercise training. International journal of endocrinology, 2014.
Hosseini, R., Bagherpoor, T., & Nemati, N. (2022). The Effect of Six Weeks Aerobic Training and Cinnamon Extract on Leptin Genes Expression in Liver Tissue of Obese Male Feeded by High Fat Diet. Journal of Animal Biology, 14(4), 91-105.
Katsiki, N., Mikhailidis, D.P., & Banach, M. (2018). Leptin, cardiovascular diseases and type 2 diabetes mellitus. Acta Pharmacologica Sinica, 39(7), 1176-1188.
Khakdan, S., Delfan, M., Heydarpour Meymeh, M., Kazerouni, F., Ghaedi, H., Shanaki, M., Kalaki-Jouybari, F., Gorgani-Firuzjaee, S., & Rahimipour, A. (2020). High-intensity interval training (HIIT) effectively enhances heart function via miR-195 dependent cardiomyopathy reduction in high-fat high-fructose diet-induced diabetic rats. Archives of physiology and biochemistry, 126(3), 250-257.
Kraemer, R., Acevedo, E., Synovitz, L., Hebert, E., Gimpel, T., & Castracane, V. (2001). Leptin and steroid hormone responses to exercise in adolescent female runners over a 7-week season. European journal of applied physiology, 86, 85-91.
Longo, M., Zatterale, F., Naderi, J., Parrillo, L., Formisano, P., Raciti, G.A., Beguinot, F., & Miele, C. (2019). Adipose tissue dysfunction as determinant of obesity-associated metabolic complications. International journal of molecular sciences, 20(9), 2358.
Marroqui, L., Gonzalez, A., Neco, P., Caballero-Garrido, E., Vieira, E., Ripoll, C., Nadal, A., & Quesada, I. (2012). Role of leptin in the pancreatic β-cell: effects and signaling pathways. Journal of molecular endocrinology, 49(1), R9-R17.
Meek, T. H., & Morton, G. J. (2016). The role of leptin in diabetes: metabolic effects. Diabetologia, 59(5), 928-932.
Min, T., & Stephens, J. W. (2015). Targeting abdominal obesity in diabetes. Diabetes Management, 5(4), 301.
Molina, A., Vendrell, J., Gutiérrez, C., Simón, I., Masdevall, C., Soler, J., & Gómez, J. M. (2003). Insulin resistance, leptin and TNF-α system in morbidly obese women after gastric bypass. Obesity Surgery, 13(4), 615-621.
Peng, B.-y., Wang, Q., Luo, Y.-h., He, J.-f., Tan, T., & Zhu, H. (2018). A novel and quick PCR-based method to genotype mice with a leptin receptor mutation (db/db mice). Acta Pharmacologica Sinica, 39(1), 117-123.
Shadegan, P. A., Khajehlandi, A., & Mohammadi, A. (2020). Effect of Aerobic Training and Crocin Consumption on Bax Gene Expression in the Hippocampal Tissue of Ovariectomized Rats. Gene, Cell and Tissue, 7(3).
Shaw, J. E., Sicree, R. A., & Zimmet, P. Z. (2010). Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes research and clinical practice, 87(1), 4-14.
Shojaei, M. (2024). A Systematic Review of the Relationship Between Sex Hormones and Leptin and Insulin Resistance in Men. Eurasian Journal of Chemical, Medicinal and Petroleum Research, 3(2), 443-453.
Singh, R. G., Pendharkar, S. A., Gillies, N. A., Miranda-Soberanis, V., Plank, L. D., & Petrov, M. S. (2017). Associations between circulating levels of adipocytokines and abdominal adiposity in patients after acute pancreatitis. Clinical and experimental medicine, 17, 477-487.
Thorand, B., Zierer, A., Baumert, J., Meisinger, C., Herder, C., & König, W. (2010). Associations between leptin and the leptin/adiponectin ratio and incident Type 2 diabetes in middle‐aged men and women: results from the MONICA/KORA Augsburg Study 1984–2002. Diabetic medicine, 27(9), 1004-1011.
Triantafyllou, G. A., Paschou, S. A., & Mantzoros, C. S. (2016). Leptin and hormones: energy homeostasis. Endocrinology and Metabolism Clinics, 45(3), 633-645.
Uysal, N., Agilkaya, S., Sisman, A. R., Camsari, U. M., Gencoglu, C., Dayi, A., Aksu, I., Baykara, B., Cingoz, S., & Kiray, M. (2017). Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats. Journal of chemical neuroanatomy, 81, 27-33.
Vasilenko, M. A., Kirienkova, E. V., Skuratovskaia, D. A., Zatolokin, P. A., Mironyuk, N. I., & Litvinova, L. S. (2017, July). The role of production of adipsin and leptin in the development of insulin resistance in patients with abdominal obesity. In Doklady Biochemistry and Biophysics (Vol. 475, pp. 271-276). Pleiades Publishing.
Yazdani, F., Shahidi, F., & Karimi, P. (2020). The effect of 8 weeks of high-intensity interval training and moderate-intensity continuous training on cardiac angiogenesis factor in diabetic male rats. Journal of physiology and biochemistry, 76, 291-299.
Zhu, J. Z., Zhao, C., Sui, Y. L., Liu, Y. Y., & Qiao, Y. B. (2020). Effects of leptin on lipid metabolism and inflammatory factors in diabetic rats. Zhongguo Ying Yong Sheng li xue za zhi= Zhongguo Yingyong Shenglixue Zazhi=. Chinese Journal of Applied Physiology, 36(3), 197-201.
Zulfania, A. K., Tahir Ghaffar, A. K., & SURO, M. (2020). Correlation between serum leptin level and Body mass index (BMI) in patients with type 2 diabetes Mellitus. JPMA, 10.
)