نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، گروه زیست‌شناسی، دانشکده علوم- دانشگاه پیام نور، تهران، ایران
2 دانشیار، گروه زیست‌شناسی، دانشکده علوم- دانشگاه پیام نور، تهران، ایران
چکیده
گونه‌های فعال اکسیژن عوامل سیتوتوکسیک هستند که موجب آسیب اکسیداتیو می‌شوند. بیسفنول A یک آلاینده سمی، اکسیداتیو و شبه‌استروژنی است. گیاه خرفه یک محصول امیدوارکننده طبیعی است که می‌تواند برای جلوگیری از بیماری های مزمن با منشأ استرس اکسیداتیو مفید واقع شود. در این مطالعه 49 سر رت نر بالغ (10±250گرم) به‌طور تصادفی به هفت گروه هفت‌تایی تقسیم شدند؛ گروه کنترل، شاهد (500 میکروگرم روغن ذرت/ کیلوگرم وزن بدن)، گروه دریافت‌کننده سم (500 میکروگرم بیسفنول A/ کیلوگرم وزن بدن)، کنترل مثبت (100 میلی‌گرم عصاره هیدروالکلی گیاه خرفه/ کیلوگرم وزن بدن)، سه گروه تیمار دریافت‌کننده خرفه (500 میکروگرم بیسفنول A+ 50، 100، 150 میلی‌گرم عصاره هیدروالکلی گیاه خرفه/ کیلوگرم وزن بدن). تمام تزریقات از طریق روش درون صفاقی و طی 14 روز پیاپی انجام شد. سپس تمامی حیوانات با کلرال هیدرات بی‌هوش شده و نمونه‌های کبدی جهت تهیه هموژن بافتی جداسازی شدند تا سنجش آنزیم‌ها آنتی‌اکسیدانی شامل TAC، CAT، GPx، GSH و MDA در این بافت انجام شود. آنالیز داده‌ها در نرم‌افزار آماری SPSS توسط روش ANOVA با تست تعقیبی توکی انجام شد. تجزیه و تحلیل داده‌ها حاکی از آن است که تجزیه و تحلیل داده‌ها حاکی از آن است که مقادیر MDA، TAC، CAT، GSH و GPX در گروه‌های تیمار با خرفه نسبت به گروه بیسفنول تعدیل شده‌اند که این امر حاکی از آن است که عصاره خرفه می‌تواند سمیت بافت کبدی ناشی از بیسفنول A را بهبود بخشد.
کلیدواژه‌ها
Alam, M, A.; Juraimi, A. S.; Rafii, M. Y.; Hamid, A. A.; Aslani, F.; Alam M. Z. (2015). Effects of salinity and salinity-induced augmented bioactive compounds in purslane (Portulaca oleracea L.) for possible economical use. Food Chemistry; 169: 439-447.
Alam, M.; Juraimi, A. S.; Rafii, M. Y.; Abdul Hamid, A.; Aslani, F.; Hasan, M. M.; et al (2014). Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected purslane (Portulaca oleracea L.) accessions. BioMed research international; 2014. https://doi.org/10.1155/2014/296063
Asl, Z. S.; Malekirad, A. A.; Abdollahi, M.; Bakhshipour, A.; Dastjerdi, H. A.; Mostafalou, S.; et al (2014). Effects of the Mixture of Cichorium intybus L. and Cinnamomum zeylanicum on hepatic enzymes activity and biochemical parameters in patients with nonalcoholic fatty liver disease. Health; 6(11):1212-1217.
Aydoğan, M.; Korkmaz, A.; Barlas, N.; Kolankaya, D. (2008). The effect of vitamin C on bisphenol A, nonylphenol and octylphenol induced brain damages of male rats. Toxicology; 249(1): 35-39.
Bindhumol, V.; Chitra, K.C.; Mathur, P.P. (2003). Bisphenol A induces reactive oxygen species generation in the liver of male rats. Toxico; 188: 117-124.
Björnsdotter, M.K.; Boer, J.D.; Ballesteros-Gómez, A. (2017). Bisphenol A and replacements in thermal paper: a review. Chemosphere; 182: 691-706. DOI: 10.1016/j.chemosphere.2017.05.070
Changizi-Ashtiyani, S.; Zarei, A.; Taheri, S.; Rasekh, F.; Ramazani, M. (2013). The effects of Portulaca oleracea alcoholic extract on induced hypercholesterolemia in rats. Zahedan J Res Med Sci ;15(6): 34-39. [in Persian]
Dkhil, M. A.; Moniem, A. E.; Al-Quraishy, S.; Saleh, R. A. (2011). Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action. J Med Plants Res; 5(9): 1589-1563.
Fani, A.; Malekirad, A.; Fani, I. (2001). On the benefit of Cinnamomum zeylanicum for radiology staff. VIRTUAL; 1(1): 384-389.
Fazli, D.; Malekirad, A.; Bayrami, M.; Shariatzadeh, S. M.; Karkhaneh, A. (2009). The effect of pomegranate juice (Punica granatum L.) on the oxidative stress of 15-17 year old girls in Arak. Journal of Shahrekord Uuniversity of Medical Sciences; 10.
Geens, T.; Aerts, D.; Berthot, C.; Bourguignon, J. P.; Goeyens, L.; Lecomte, P.; et al. (2012a). A review of dietary and non-dietary exposure to bisphenol-A. Food Chem. Toxicol; 50(10): 3725-3740. DOI: 10.1016/j.fct.2012.07.059
Geens, T.; Neels, H.; Covaci, A. (2012b). Distribution of bisphenol-A, triclosan and n-nonylphenol in human adipose tissue, liver and brain. Chemosphere; 87(7): 796-802. DOI: 10.1016/j.chemosphere.2012.01.002
Gharibi, S.; Dilmaghanian, A.; Sadighara, P.; Fard, R.M.N. (2013). The effect of bisphenol a on oxidative stress indices and pathological changes in the brain of chicken embryos. World Appl Sci J; 26: 345-51.
Ghorbanian, B.; Saberi, Y.; Azali Alamdari, K.; Shokrollahi, F.; Mohammadi, H. (2019). The Effects of Portulaca Supplementation on Antioxidant Biomarkers and Oxidative Stress in non-Active Girls. J. Med. Plants; 4 (72): 255-263.
Gong, Y.; Han, X.D. (2006). Nonylphenol-induced oxidative stress and cytotoxicity in testicular sertoli cells. Reprod. Toxicol; 22: 623-630.
Jain, S.; Kumar, C.M.; Suranagi, U.D.; Mediratta P.K. (2011). Protective effect of N-acetylcysteine on bisphenol A-induced cognitive dysfunction and oxidative stress in rats. Food and chemical toxicology; 49(6): 1404-1409.
Kabuto, H.; Amakawa, M.; Shishibori, T. (2004). Exposure to bisphenol A during embryonic/ fetal life and infancy increases oxidative injury and causes underdevelopment of the brain and testis in mice. Life Sci; 74: 2931-2940.
Kabuto, H.; Hasuike, S.; Minagawa, N.; Shishibori T. (2003). Effects of bisphenol A on the metabolisms of active oxygen species in mouse tissues. Environmental research; 93(1): 31-35.
Kajta, M.; Wójtowicz, A. K. (2013). Impact of endocrine-disrupting chemicals on neural development and the onset of neurological disorders. Pharmacological Reports; 65(6): 1632-1639.
Karasek, M. (2007). Does melatonin play a role in aging processes? Journal of physiology and pharmacology; 58(6): 105-113.
Kobroob, A.; Peerapanyasut, W.; Chattipakorn, N.; Wongmekiat, O. (2018). Damaging effects of bisphenol A on the kidney and the protection by melatonin: emerging evidences from in vivo and in vitro studies. Oxidative medicine and cellular longevity. DOI: 10.1155/2018/3082438
Kumar, V.L.; Padhy, B.M. (2011). Protective effect of aqueous suspension of dried latex of Calotropisprocera against oxidative stress and renal damage in diabetic rats. Biocell; 35(3): 63-9.
Lin, Y.; Zeng X. G.; Wu D. S.; Wang X. (2006). Study on bisphenol A induced primary cultured mesencephalic neuronal cell injury by oxidative stress. Wei sheng yan jiu. Journal of hygiene research; 35(4): 419-422.
Malekirad, A.A.; Hosseini, N.; Bayrami, M.; Hashemi, T.; Rahzani, K.; Abdollahi, M. (2011). Benefit of Lemon Verbena in Healthy Subjects; Targeting Diseases Associated with Oxidative Stress. Asian Journal of Animal and Veterinary Advances; 6(9): 953-957.
Miladi-Gorgi, H.; Vafaei, A.A.; Taherian, A.A.; et al. (2009). The Effects of Aqueous Extracts of Purtulaca oleracea on Withdrawal Syndrome in Mice. Journal of Medicinal Plants; 8: 51-7. (in Persian)
Nakagawa, Y.; Tayama S. (2000). Metabolism and cytotoxicity of bisphenol A and other bisphenols in isolated rat hepatocytes. Arch Toxicol; 74(2): 99-105.
Nemzer, B.; Al-Taher, F.; Abshiru N. (2020). Phytochemical composition and nutritional value of different plant parts in two cultivated and wild purslane (Portulaca oleracea L.) genotypes. Food chemistry; 320: 126621.
Pablos, M. I.; Agapito, M. T.; Gutierrez, R.; Recio, J. M.; Reiter, R.J.; Barlow‐Walden, L.; et al. (1995). Melatonin stimulates the activity of the detoxifying enzyme glutathione peroxidase in several tissues of chicks. J Pineal Re;19(3): 111-5.
Palaniswamy, U. R.; McAvoy, R. J.; Bible, B.B. (2001). Stage of harvest and polyunsaturated essential fatty acid concentrations in purslane (Portulaca oleraceae) leaves. Journal of Agricultural and Food Chemistry; 49(7): 3490-3493.
Petropoulos, S.; Karkanis, A.; Martins, N.; Ferreira I. C. (2016). Phytochemical composition and bioactive compounds of common purslane (Portulaca oleracea L.) as affected by crop management practices. Trends in food science & technology; 55; 1-10.
Raja, G. L.; Lite, C.; Subhashree, K. D.; Santosh, W.; Barathi, S. (2020). Prenatal bisphenol-A exposure altered exploratory and anxiety-like behaviour and induced non-monotonic, sex-specific changes in the cortical expression of CYP19A1, BDNF and intracellular signaling proteins in F1 rats. Food and Chemical Toxicology; 142: 111442.
Rashed, A. N.; Afifi, F. U.; Disi A. M. (2003). Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol; 88(2-3): 131-6.
Reiter, R. J. (1991). Pineal melatonin: Cell biology of its synthesis and of its physiological interactions. Endocr Rev; 12(2): 151-80.
Reiter, R. J. (1995). Oxidative processes and antioxidative defense mechanisms in the aging brain. The FASEB journal; 9(7): 526-533.
Rochester, J. R.; Bolden, A. L. (2015). Bisphenol S and F: A systematic review and comparison of the hormonal activity of Bisphenol A substitutes. Environ Health Perspect; 74(9): 1-33.
Rogers, J. A.; Metz, L.; Yong V. W. (2013). Endocrine disrupting chemicals and immune responses: A focus on bisphenol-A and its potential mechanisms. Molecular immunology; 53(4): 421-430.
Ronnberg, L.; Kauppila, A.; Leppaluoto, J.; Martikainen, H.; Vakkuri, O. (1990). Circadian and seasonal variation in human preovulatory follicular fluid melatonin concentration. J Clin Endocrinol Metab; 71(2): 492-6.
Rubin, B. S.; Lenkowski, J. R.; Schaeberle, C. M.,;Vandenberg, L. N.; Ronsheim, P. M.; Soto A.M. (2006). Evidence of altered brain sexual differentiation in mice exposed perinatally to low, environmentally relevant levels of bisphenol A. Endocrinology; 147(8): 3681-3691. DOI: 10.1210/en.2006-0189
Saffaryazdi, A.; Ganjeali, A.; Farhoosh, R.; Cheniany M. (2020). Culture Optimization for In Vitro Callogenesis in Purslane (Portulaca oleracea) and Effect of Yeast Extract on Antioxidant Compounds. Journal Of Horticultural Science; 34(1): 107-118. (in Persian)
Saffaryazdi, A.; Ganjeali, A.; Farhoosh, R.; Cheniany M. (2020). Variation in phenolic compounds, α-linolenic acid and linoleic acid contents and antioxidant activity of purslane (Portulaca oleracea L.) during phenological growth stages. Physiology and Molecular Biology of Plants; 26(7); 1519-1529.
Sen, C.K. (1999). Glutathione homeostasis in response to exercise training and nutritional supplements. In Stress adaptation, prophylaxis and treatment. Springer; 31-42
Sharma, A.; Vijayakumar, M.; Rao, C. V.; Unnikrishnan, M.K.; Reddy G.D. (2009). Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats. Journal of Complementary and Integrative Medicine; 6(1).
Vogel, S. A. (2009). The politics of plastics: the making and unmaking of bisphenol a "safety". Am. J. Public Health; 99(3): 559-566. DOI: 10.2105/AJPH.2008.159228
Wang, C.Q.; Yang, G.Q. (2010). Betacyanins from Portulaca oleracea L. ameliorate cognition deficits and attenuate oxidative damage induced by D-galactose in the brains of senescent mice. Phytomedicine; 17(7): 527-32.
Wang, I. J.; Chen, C. Y.; Bornehag, C. G. (2016). Bisphenol A exposure may increase the risk of development of atopic disorders in children. Int. J. Hyg. Environ. Health; 219(3); 311-316. DOI: 10.1016/j.ijheh.2015.12.001
Wiersielis, K. R.; Adams, S.; Yasrebi, A.; Conde, K.; Roepke, T. A. (2020). Maternal exposure to organophosphate flame retardants alters locomotor and anxiety-like behavior in male and female adult offspring. Hormones and behavior; 122: 104759.
Xiang, L.; Xing, D.; Wang, W.; Wang, R.; Ding, Y.; Du, L. (2005). Alkaloids from Portulaca oleracea L. Phytochemistry; 66(21): 2595-2601.
Yang, Z.; Liu, C.; Xiang, L.; Zheng, Y. (2009). Phenolic alkaloids as a new class of antioxidants in Portulaca oleracea. Phytotherapy Research; 23(7): 1032-5.
Young, I.S.; Woodside, J. (2001). Antioxidants in health and disease. Journal of clinical pathology; 54(3): 176-186.
Zareei, M. A.; Saberi, M.; PIR, Z. J.; Golmanesh, L. (2005). Investigation of Acetaminophen Induction Effects on GSH Concentration by N-Acetylcysteine and 2-Oxothrazolidin-4-Carboxylate (OTC) and Its Protective Effect on sulfure Mustard Injures in Hf2ff Cells. Kowsar medical journal; 10(3): 175-182.
Zarei, A,; Changizi Ashtiyani, S.; Taheri, S. (2014). The effects of hydroalcoholic extract of Portulaca Oleracea on the serum concentreation of Hepatic enzymes in Rats. Iran South Med J; 17(5): 889-99. (in Persian)
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