نوع مقاله : مقاله پژوهشی
نویسنده
پژوهشکده آرتمیا و آبزی‌پروری، دانشگاه ارومیه، ارومیه، ایران
10.30473/eab.2023.68141.1912
چکیده
در این پژوهش، ماهیان دیپلوئید، تریپلوئید و تتراپلوئید قزل‌آلای رنگین‌کمان از نظر رشد، بقا و ویژگی‌های گلبول قرمز مورد مقایسه قرار گرفتند. برای تولید ماهیان تریپلوئید، تخم‌های لقاح یافته تحت شوک گرمایی 5/26 درجه سانتی‌گراد در قالب سه تیمار: 1- دوبار شوک یک دقیقه‌ای در 15 و 30 دقیقه بعد از لقاح، 2- یک‌بار شوک 15 دقیقه‌ای در 15 دقیقه بعد از لقاح و 3- یک‌بار شوک 12 دقیقه‌ای در 20 دقیقه بعد از لقاح، قرار گرفتند. برای تولید ماهیان تتراپلوئید، تخم‌های لقاح یافته تحت شوک گرمایی 28 درجه سانتی‌گراد در قالب سه تیمار: 1- شوک 10 دقیقه‌ای در 59 ساعت-درجه بعد از لقاح، 2- شوک 10 دقیقه‌ای در 66 ساعت-درجه بعد از لقاح و 3- شوک 10 دقیقه‌ای در 72 ساعت-درجه بعد از لقاح، قرار گرفتند. در تریپلوئیدسازی، شوک گرمای 5/26 درجه سانتی‌گراد در 20 دقیقه بعد از لقاح به‌مدت 12 دقیقه با درصد تفریخ 6/66 درصد و نرخ تریپلوئیدسازی 87 درصد بهتر از سایر گروه‌ها بود. در تتراپلوئیدسازی، شوک گرمای 28 درجه سانتی‌گراد در 59 ساعت-درجه بعد از لقاح به‌مدت 10 دقیقه با درصد تفریخ 9/54 درصد و نرخ تتراپلوئیدسازی 94/7 درصد نسبت به سایر تیمارها مناسب‌تر بود. ماهیان تریپلوئید از نظر رشد با دیپلوئیدها اختلاف معنی‌داری نداشتند اما رشد ماهیان تتراپلوئید به‌طور معنی‌داری کمتر از دیپلوئیدها بود. به‌طورکلی، در این مطالعه علاوه بر بهینه‌سازی روش‌های تریپلوئید و تتراپلوئیدسازی، استفاده از طول، عرض و مساحت سلولی گلبول‌های قرمز، به‌عنوان یک روش کاربردی و کم هزینه برای تفکیک ماهیان دیپلوئید، تریپلوئید و تتراپلوئید قزل‌آلای رنگین‌کمان مورد تائید قرار گرفت.
کلیدواژه‌ها
موضوعات
lcantar-Vazquez, J. P., Dumas, S., Puente-Carreon, E., Pliego-Cortés, H. S., & Peña, R. (2008). Induction of triploidy in spotted sand bass (Paralabrax maculatofasciatus Steindachner, 1868) by cold shock. Aquacaltural Research, 39, 59-63.
Bahrami Babaheydari, S., Keyvanshokooh, S., Dorafshan, S., & Johari, S. (2017). Effects of tetraploidization on hatching, survival, growth performance and proximate composition in rainbow trout (Oncorhynchus mykiss). Veterinary Research & Biological Products, 30(4), 231-240. (in Persian).
Bekcan, S., Atar, H. H., & Yavuzcan, H. (2016). The survival rate of rainbow trout (Oncorhynchus mykiss, walbaum) at the stages of eyed eggs, larvae and fry in tetraploid applications. Journal of Applied Biological Sciences, 10(3), 20-23.
Bencsik, I., Pacala, N., Dumitrescu, G., Dronca, D., Stanculet, J., & Petculescu-Ciochina, L. (2013). Triploidy determination in rainbow trout (Oncorhynchus mykiss) based on erythrocytes dimensions. Animal Science and Biotechnologies, 46(1), 113-117.
Benfey, T. J., & Sutterlin, A. M. (1984a). Triploidy induced by heat shock and hydrostatic pressure in landlocked Atlantic salmon (Salmo salar L.). Aquaculture, 36, 359-367.
Benfey, T. J., & Sutterlin, A. M. (1984b). Growth and gonadal development in triploid landlocked Atlantic salmon Salmo salar. Canadian Journal of Fisheries and Aquatic Sciences, 41, 1387-1392.
Cal, R. M., Vidal, S., Gómez, C., Álvarez-Blázquez, B., Martínez, P., & Piferrer, F. (2006). Growth and gonadal development in diploid and triploid turbot (Scophthalmus maximus). Aquaculture, 251, 99-108.
Chourrout, D., Chevassus, B., Krieg, F., Happe, A., Burger, G., & Renard, P. (1986). Production of second generation triploid and tetraploid rainbow trout by mating tetraploid males and diploid females—potential of tetraploid fish. Theoritcal and Applied Genetic, 72, 193-206.
Diaz, N. F., Iturra, P., Veloso, A., Estay, F., & Colihueque, N. (1993). Physiological factors affecting triploid production in rainbow trout, Oncorhynchus mykiss. Aquaculture, 114, 33-40.
Doğankaya, L., & Bekcan, S. (2014). The effects of pressure and heat shocks induced at different times after fertilization on triploid production in rainbow trout (Oncorhynchus mykiss, W. 1792). Eğirdir Su Ürünleri Fakültesi Dergisi, 10(2), 35-43.
Dorafshan, S., Vafaei Saadi, A., Nekoeifard, A. (2014). Optimal heat shock condition for tetraploidy induction in rainbow trout (Oncorhynchus mykiss). Journal of Veterinary Research, 69(4), 411-421. (in Persian).
Dunhum, R. A. (2004). Aquqculture Fisheries Biotecnology: Genetic Approaches. Cambridge. CABI Publishing.
Espinosa, E., Josa, A., Gil, L., & Marti, J. I. (2005). Triploidy in rainbow trout determined by computer-assisted analysis. Journal of Experimental Zoology, 303A, 1007-1012.
Fauconneau, B., Kaushik, S. J., & Blanc, J. M. (1989). Uptake and metabolization of dissolved compounds in rainbow trout fry. Comparative Biochemistry and Physiology, 93, 839-843.
Felip, A., Piferrer, F., Zanuy, S., & Carrillo, M. (2001). Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons. Journal of Fish Biology, 58, 76-88.
Gandomkar, H. A., Rastiannasab, A., Nazari, S., Kazemi, E., & Jahanabad, J. M. (2020). Efficiency and optimization of heat shock in production of rainbow trout tetraploid. Scientific Research Journal of Animal Environment, 12(4), 335-343. (in Persian).
Gjedrem, T., Robinson, N., & Rye, M. (2012). The importance of selective breeding in aquaculture to meet future demands for animal protein: a review. Aquaculture, 350-353, 117-129.
Güner, M., Peker, Z., & Altunok, M. (2016). Optimization of thermal shock for poliplody induction in rainbow trout (Oncorhynchus mykiss) under photoperiodic control of spawning. Turkish Journal of Fisheries and Aquatic Sciences, 16, 797-804.
Horstgen-Schwark, G. (1993). Initiation of tetraploid breeding line development in rainbow trout, Oncorhynchus mykiss. Aquaculture and Fisheries Management, 24, 641-652.
Howell, W. M., & Black, D. A. (1980). Controlled silver staining of nucleolus organizer with a protective colloidal developer: a 1-step method. Expereintia, 36(8), 1014-1015.
Hwang, G. D. (2012). Inland fisheries research institute yearly research report. Korea: Chungcheongbuk-do.
Irani, A., & Agh, N. (2020). Rainbow trout larvae production in an airlift-based recirculating system. Aquaculture, 518, 734831.
Kalbasi, M. R., Bagheri, A., Pourkazemi, M., & Abdolhai, H. (2004). Induction of t etraploidy in rainbow trout (Onchorhynchus mykiss) by heat shock. Iranian Scientific Fisheries Journal, 12(4), 143-152. (in Persian).
Kalbassi, M. R., & Johari S. A. (2008). A Study on the Production Possibility of All-Female Triploid Rainbow Trout (Oncorhynchus mykiss). Journal of Crop Production and Processing, 12(44), 269-278. (in Persian).
Kizak, V., Güner, Y., Türel, M., & Kayim, M. (2013). Comparison of growth performance, gonadal structure and erythrocyte size in triploid and diploid brown trout (Salmo trutta fario L., 1758). Turkish Journal of Fisheries and Aquatic Sciences, 13, 571-80.
Kim, H. S., Chung, K., & Son, J. (2017). Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout. Fisheries and Aquatic Sciences, 20(1), 1-7.
Johari, S. A., & Kalbassi., M. R. (2007). Investigation of red blood cell alterations in triploid rainbow trout (Oncorhynchus mykiss). Iranian Journal of Biology, 19(4), 492-495. (in Persian).
Johnstone, R. (1985). Induction of triploidy in Atlantic salmon by heat shock. Aquaculture, 49, 133-139.
Lamatsch, D. K., Steinlein, C., Schmid, M., & Schartl, M. (2000). Non-invasive determination of genome size and ploidy level in fishes by flow cytometry detection of triploid Poecilia formosa. Cytometry, 39, 91-95.
Lincoln, R. F., & Scott, A. P. (1983). Production of all-female triploid rainbow trout. Aquaculture, 30, 375-380.
Liu, S., Sezakki, D., Hashimoto, K., Kobayashi, H., & Nakamura, M. (1978). Simplified techniques for determination of polyploidy in ginbuna, Carassius auratus langsdorfi. Bulletin of the Japanese Society for the Science of Fish, 44, 601-606.
Maxime, V. (2008). The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish and Fisheries, 9(1), 67-78.
Olele, N. F., & Tiguiri, O. H. (2013). Optimization of triploidy induction and growth performance of Claria anguillarias (African catfish) using cold shock. Academic journal of interdisciplinary studies, 2, 189-96.
Pandian, T. J., & Koteeswaran, R. (1998). Ploidy induction and sex control in fish. Hydrobiologia, 384, 167-243.
Piferrer, F., Beaumont, B., Falguiere, J. C., Flajshans, D. M., Haffray, P. E., & Colombo, L. (2009). Polyploy fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture, 293, 125-56.
Porto-Foresti, F., Oliveira, C., Tabata, Y. A., Rigolino, M. G., & Foresti, F. (2002). NORs inheritance analysis in crossings including individuals from two stocks of rainbow trout (Oncorhynchus mykiss). Hereditas, 136,227-30.
Pradeep, P. J., Srijaya, T. C., Jose, D., Papini, A., Hassan, A., & Chatterji, A. K. (2011). Identification of diploid and triploid red tilapia by using erythrocyte indices. Caryologia, 64, 485-492.
Shimizu, Y., Oshiro, T., & Sakaizumi, M. (1993). Electrophoretic studies on diploid, triploid and tetraploid forms of the Japanese silver crucian carp, Carassius auratus langsdorfi. Japanese Journal of Ichthyology, 40, 65-75.
Small, S. A., & Benfey, T. J. (1987). Cells size in triploid salmon. J Exp Zool, 241, 339-342.
Solar, I. I., Donaldson, E. M., George, A., & Hunter, G. A. (1984). Induction of triploidy in rainbow trout (salmo gairdneri Richardson) by heat shock, and investigation of early growth. Aquaculture, 42, 57-67.
Sourinezhad, I., & Kalbassi, M. R. (2011). Investigation of growth indices of all female and mixed sex diploid and triploid rainbow trout Oncorhynchus mykiss in the second year of culture. Iranian Journal Of Biology, 24(4), 517-527. (in Persian).
Sourinezhad, I., Kalbasi, M. R., Khodabandeh, S., & Rezaei, M. (2009). Comparative study of ovary development of all female diploid and tnploid rainbow trout Oncorhynchus mykiss in the second year of culture. Iranian Journal Of Biology, 22(1), 111-123. (in Persian).
Thorgaard, G. H. (1983). Chromosome sex manipulation and sex control in fish. In: Hoar, W., Randall, D. and Donaldson, E., editors. Fish physiology, 9, New York, NY: Academic Press.
Thorgaard, G. H., & Disney, G. (1990). Chromosome preparation and analysis. In: Schreck, C., Moyle, P., editors. Methods for fish biology. Bethesda, MD: American Fisheries Society.
Tiwary, B. K., Kirubagaran, R., & Ray, A. K. (1997). Induction of triploid by cold shock in catfish, Heteropneustes fossilis (Bloch). Asian Fisheries Science, 10, 123-129.
Tiwary, B. K., Kirubagaran, R., & Ray, A. K. (2004). The biology of triploid fish. Reviews in Fish Biology and Fisheries, 14, 391-402.
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