نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد، گروه زیست‌شناسی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران
2 استادیار، گروه کشاورزی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران
3 استادیار، گروه زیست‌شناسی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران
چکیده
طولانی‌شدن زمان بهبود زخم، زمینه ایجاد عفونت‌های مزمن را مستعد می‌کند. لذا دستیابی به روش‌هایی پیشگیریکننده عفونت به‌منظور تسریع روند التیام زخم یکی از نیازهای روز بشر محسوب می‌شود. در این مطالعه اثرات عصاره خیار دریایی گونه Holothuria leucospilota بر روی ترمیم زخم حاد ایجادشده در پوست رت ویستار نر بررسی شد. بدین منظور عصاره متانولی از نمونه‌های خیار دریایی جمع‌آوری‌شده از عمق 10 الی 15 متری سواحل سوزا در جزیره قشم با استفاده از روتاری تهیه شد که در ادامه به‌منظور تهیه پماد، 5/0 و 1 گرم از عصاره به‌دست‌آمده به پماد پایه اضافه شد. سپس 40 عدد رت با میانگین وزنی 5± 185گرم را به چهار گروه شاهد، گروه شم، گروه تیمارشده با پماد 5/0 درصد و گروه تیمارشده با پماد 1 درصد تقسیم شدند. بر روی پوست تمامی رتها زخمی به عمق تمام پوست و قطر 1± 6 میلی‌متر بوسیله پانچ بیوبسی ایجاد گردید و هر گروه را با دوز پماد تهیه شد به‌مدت 13 روز و یک نوبت در روز به میزان 1/0 سیسی تیمار شدند. در روزهای صفر، چهار، هفت، 10 و 13 روند بهبود زخم بررسی و قطر زخمها اندازهگیری شد. براساس مشاهدات، سرعت بهبود زخم در گروههای تیمارشده با پماد 1 درصد و 5/0 درصد نسبت به گروه شاهد و شم در طی مدت مطالعه بیش‌تر بود. هم‌چنین از نظر آماری بین گروه تیمارشده با پماد 1، شم و شاهد در روزهای هفت، 10 و 13 تفاوت معنی‌داری مشاهده شد.
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