نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار گروه زیست شناسی دریا، دانشکده علوم دریایی دانشگاه دریانوردی و علوم دریایی چابهار
2 دانشجوی کارشناسی ارشد زیست شناسی دریا، دانشکده علوم دریایی دانشگاه دریانوردی و علوم دریایی چابهار
چکیده
چکیده
خارپوستان گروهی قدیمی از بیمهرگان دریایی بوده که منبع غنی ترکیبات ضدباکتری با مکانیسم فعالیت بالا هستند. هدف از مطالعه حاضر، بررسی اثر ضدباکتریایی پوسته توتیای دریایی گونه Echinometra mathaei در دوزهای مختلف، روی چند سویه باکتری بیماریزای انسانی است. پس از انجام نمونهبرداری و انتقال نمونهها به آزمایشگاه، جانور تشریح و پوسته آن جدا و پس از شستشو و آسیاب شدن، توسط حلال کلروفرم عصارهگیری صورت گرفت و غلظتهای مختلف 25/11-25/1 میلیگرم بر میلیلیتر تهیه شد و جهت تعیین فعالیت ضدباکتریایی آنها، روی باکتریهای Staphylococcus aureus، Enterococcus faecalis، Escherichia coli و Klebsiella pneumonia از تست انتشار از دیسک استفاده شد. نتایج نشان داد که غلظتهای مختلف عصاره تهیه شده در باکتریهای مختلف دارای اختلاف معنیداری هستند) 05/0(p
کلیدواژه‌ها
Abubakar, L.; Mwangi, C.; Uku, J.; Ndirangu, S.; (2012). Antimicrobial activity of various extract of the sea urchin Tripneustes gratilla (Echinoidea). African Journal of Pharmacology and Therapeutics; 1(1): 19-23.
Adibpour, N.; Nasr, F.; Nematpour, F.; Shakouri, A.; Ameri, A.; (2014). Antibacterial and Antifungal Activity of Holoyjuria leucospilota Isolated From Persian Gulf and Oman Sea. Jundishapur j Microbiol; 7(1): e8708.
Ageenko, V.N.; Kiselev, V.K.; Nelly, A.O.; (2011). Epression Of Pigments Cell-Specific Genes In The Ontogenesis of The Sea Urchi Strongylocentrotus Intermedius. Hindawi Publishing Corporation; Article ID 730356, 9 pages.
Asleian, H.; Kamrany, A.; Yousefzadey, M.; Keshavarz, M.; (2014). Antibacterial activity of various extracts of the sea urchin Echinometra mathaei; 37-52.
Bickmeyer, U.; Assmann M.; Kock, M.; Christian, S.; (2005). A secondary metabolite, 4,5dibromopyrrole-2-carboxylic acid , from marinesponges of the genus Agelas alters cellular calciumsignals. Environmental toxicology and pharmacology; 19: 423-427.
Blunt, JW.; Copp, B.R.; Munro, M.H.G.; Northcote, P.T.; Prinsep, M.R.; (2007). Marine natural products. Nat Prod Rep; 21: 1-49.
Briskin, D.; (2000). Medicinal Plants and Phytomedicines.Linking Plant Biochemistry and Physiology to Human Health. Plant Physiology; 124: 507-514.
Brogden, KA.; (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol; 3: 238-250.
Casas, SM.; Comesana, P.; Cao, A.; Villalba, A.; (2011). Comparison of antibacterial activity in the hemolymph of marine bivalves from Galicia (NW Spain). J. Invertebr. Pathol; 106: 343-345.
De Vries, D.J.; Hall, M.R.; (1994). Marine biodiversity as a source of chemical diversity. Drug Dev; 33: 161-173.
Hale, JD.; Hancock, RE.; (2007). Alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Expert Rev Anti Infect Ther; 5: 951-959.
Harper, M.K.; Bungi; T.S.; Copp, R.D.; James, B.S.; Lindsay, A.D.; Richardson, P.C.; Schnabel, D.; Tasdemir, R.M.; Vanwagoner, S.M.; Verbitski, M.; Ireland, C.M.; (2001). Introduction to the chemical ecology of marine natural products. In: J.B. McClintock and B.J. Baker (eds), Marine chemical ecology, marine biology; 3-69.
Haug, T.; Kjuul, AK.; Styrvold, OB.; Sandsdalen, E.; Olsen, MO.; Stensvag, K.; (2002). Antibacterial activity in Strongylocentrotus droebachiensis (Echinoidea), Cucumaria frondosa (Holothuroidea) and Asterias rubens (Asteroidea). J. Invertebr. Pathol; 81: 94-102.
Jahan, N.; Khatoon, R.; Shahzad, A.; Shahid, M.; Ahmad, S.; )2013(. Comparison of antibacterial activity of parent plant of Tylophora indica Merr. with its in vitro raised plant and leaf callus. African Journal of Biotechnology; 12(31): 4891 4896.
Jha, RK.; Zirong, Xu.; (2004). Biomedical Compounds from Marine organism. Mar Drugs; 2(3): 123-146.
Kamysz, W.; Okroj, M.; Łukasiak, J.; (2003). Novel properties of antimicrobial peptides. Acta Biochim Pol; 50: 461-469.
Lai, Y.G.; (2009). AMPed up immunity: how antimicrobial peptides have multple roles in immune defense. Trends Immunol; 30: 131-141.
Lebedev, A.; Lvanova, M.; Levitsky, D.; (2008). Iron Chelators and Free Radical Scavengers In Naturally Occuring Polyhyroxylated 1, 4-Naphthoquinones. Hemoglobin; 32(1-2): 165-79.
Nicolas, P.; (2009). Multifunctional host defense peptides: intracellular-targeting antimicrobial peptides. FEBS J; 276: 6483-6496.
Nourozy, J.; Valey, Gh.; Yousefy, H.; (2004). The effect of mutations on different methods and patterns of antibiotic resistance plasmids in E.coli and Staphylococcus aureus. Scientific Journal; 8(29): 8-1.
Ovchinnikon, T.V.; (2006). Aurelin a novel antimicrobial peptide from jellyfish Aurelia aurita with structural features of defensins and channel-blocking toxins. Biochem. Biophys. Res. Commun; 348: 514-523.
Reich, M.L.; (2006). Combrian holothurians the early fossil record and evolution of Holothuroidea. Journal Georges Ubaghs (Dijon, France: Universite de Bourgogne) dipl. geol. David Ware; 36-37.
Rinkevich, B.; (2000). Cell culture from marin invertebrates: obstacle, new approches and recent improvements. J. Biotechnol; 70: 133-153.
Schillaci, D.; Arizza, V.; (2013). Echinoderm Antimicrobial Peptides to Contrast Human Pathogens. Natural Products Chemistry & Research; 1: 2.
Service, M.; Wardlaw, A.C.; (1984). Echinochrome-A as a bactericidal substance in the coelomic fluid of Echinus esculentus (L.). Comp. Biochem. Physiol; B79: 161-165.
Shankarlal, S.; Prabu, K.; Natarajan, E.; (2011). Antimicrobial and Antioxidant Activity of Purple Sea Urchin Shell (Salmacis virgulata). American-Eurasian Journal of Scientific Research; 3: 178-181.
Stabili, L.; Lassagues, M.; Pastore, M.; (1996). Study on the antibacterial capabilities of eggs of Paracentrotus liviys (Echinodermata: Echinodea). J. Invertebr. Pathol; 67: 180-182.
Stabili, L.; Pagiliara, P.; (1994). Antibacterial protection in Marthasterias glacialis eggs-characterization of lysozyme-like activity. Comp. Biochem. Physioal; B 109, 709-713.
Uma, B.; Parvathavarthini, R.; (2014). Antibacterial Activity of Hydroalcohil Extract of Sea Urchin Temnopleurus Alexandri. Journal of Applied Research; Issue 1: 1677-1680.
Uma, B.; Paravathavarthini, R.; (2010). Antibacterial Effect of Hexane extract of Sea Urchin, Temnopleurus alexandri. International Journal of PharmTech Research; 3: 1677-1680.
Uma, B.; Parvathavarthini, R.; (2014). Antibacterial Activity of Hydroalcohil Extract of Sea Urchin Temnopleurus Alexandri. Journal of Applied Research. Volume 4- Issue:1
Venugopal, V.; (2009). Marine products for healthcare : function and bioactive nutraceutical compound from the ocean. CRC Press Taylor & Francis Group; Boca Raton, FL, USA; 23-50.
WHO (World health Organization).; (1998). The World Health Report 1998, Life in the 21st century A vision for all. Report in the Director-General. Genova, Switzerland. Pp 30-46.
Yasoda, H.N.; Chi, Z.; Zhu, K.; (2006). Probiotics and sea cucumber farming. SPC beche-de-mer Information Bulletin; 24: 4-8.
Yount, NY.; Bayer, AS.; Xiong, YQ.; Yeaman, MR.; (2006). Advances in antimicrobial peptide immunobiology. Biopolymers; 84: 435-458.
Zhou, D.Y.; Qin, L.; Zhu, B.W.; Wang, X.D.; Tan, H.; Feng Yang, J. et al.; (2011). Extraction and Antioxidant Property of Polyhydroxylated Naphthoquinone Pigments From Spines of Purple Sea Urchin Strongylocentrotus Nudus. Food Chemistry; 129(4): 1591-1597.
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