با همکاری مشترک دانشگاه پیام نور و انجمن فیزیولوژی و فارماکولوژی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران

2 گروه بیوشیمی، دانشگاه علوم پزشکی دانشگاه ‏ایران، تهران، ایران

10.30473/eab.2026.73689.1981

چکیده

بیماری کبد چرب غیرالکلی (NAFLD) یک اختلال مزمن کبدی است که با تجمع قطرات چربی (LDs) در سلول‌های کبدی مشخص می‌شود. این مطالعه با هدف بررسی تأثیر اولئات (اسید چرب غیراشباع)، پالمیتات (اسید چرب اشباع)  و ترکیب آن‌ها بر تجمع چربی، پروفایل لیپیدی و ایجاد مدل سلولی NAFLD در رده سلولی HepG2 انجام شد. مقادیر IC50 برای هر یک از اسیدهای چرب با استفاده از آزمون MTT تعیین گردید. محتوای قطرات چربی از طریق رنگ‌آمیزی Oil Red O، بررسی میکروسکوپی و تحلیل نیمه‌کمی ارزیابی شد. مقادیر تری‌گلیسیرید (TG)، کلسترول (Ch) و HDL با استفاده از کیت پارس‌آزمون اندازه‌گیری و داده‌ها با نرم‌افزار GraphPad Prism تحلیل شدند. نتایج نشان داد که تیمار با پالمیتات منجر به افزایش قابل‌توجهی در تجمع قطرات چربی و سطوح TG، Ch و HDL به‌ترتیب 1/41±2/1، 3/6±3/0 و 33/0±4/0 نسبت به سلول‌های کنترل (5/17±2/1، 5/3±7/0 و 27/0±4/0 شد. ترکیب پالمیتات و اولئات نیز باعث افزایش بیش‌تر تجمع چربی و مقادیر TG و Ch به‌ترتیب 5/43±5/1 و 1/6±9/0) گردید. برخلاف مطالعات پیشین، اولئات در این پژوهش نه‌تنها اثر حفاظتی نداشت، بلکه موجب تشدید اثرات آسیب‌زای پالمیتات شد. این یافته‌ها بر لزوم انجام مطالعات بیش‌تر در زمینه مکانیسم‌های مولکولی اثرات ترکیبی اسیدهای چرب اشباع و غیراشباع در پیشرفت NAFLD تأکید دارد.

کلیدواژه‌ها

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