نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
10.30473/eab.2024.69812.1931
چکیده
چربی خون یک مشکل جهانی و رو به افزایش در همه کشورها از جمله ایران و یکی از تهدیدهای مهم برای سلامت جامعه میباشد. چربی خون بالا به‌عنوان یک مشکل چند عاملی تحت تأثیر مجموعه عوامل مختلف، به‌ویژه عوامل متابولیکی و ژنتیکی قرار دارد. هدف از این مطالعه، بررسی پلی‌مورفیسم rs2016520 در ژن PPARD و ارتباط آن با ﺳﻄﺢ لیپوپروتئین با چگالی پایین (LDL) میباشد. خونگیری از 137 فرد شامل 83 زن و 54 مرد ایرانی شامل 79 فرد سالم یا شاهد (LDL نرمال) (7/57 درصد) و 58 فرد با LDL بالاتر از 130 (3/42 درصد) در محدوده سنی 18 تا 60 سال با گرفتن رضایتنامه، انجام شد.DNA توسط کیت آماده استخراج شد. غلظت و کیفیتDNA جداشده به‌وسیله دستگاه اسپکتروفتومتری و ژل آگارز سنجیده شد. شناسایی ژنوتیپ‌ها با روش PCR-RFLP انجام شد. میزان 6 درصد فراوانی ژنوتیپ‌ها در گروه باLDL نرمال: 8/3 درصد برای CC، 7/36 درصد برای CT و 5/59 درصد برای ژنوتیپ TT و در گروه باLDL بالاتر از 130: 1/12 درصد برای CC، 9/37 درصد برای CT و 0/50 درصد برای ژنوتیپ TT بود. اختلاف فراوانی ژنوتیپ‌ها بین دو گروه با LDL نرمال و گروه با LDL بالاتر از 130 معنی‌دار نبود (05/0 < P). 56/2 درصد از نمونه‌ها، BMI پایینتر از 25 و 8/43 درصد، BMI بالاتر از 25 داشتند. نتایج مطالعه حاضر ارتباط معنیداری میان پلی‌مورفیسم rs2016520 و سطح LDL نشان نداد.
کلیدواژه‌ها
موضوعات
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