Shahriar Saeidian; Zahra Baghaei Far; Zivar Parvanak; Mokhtar Fathi
Abstract
Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential ...
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Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential of these molecules as biomarkers for disease diagnosis. Also, changes in the expression of lncRNAs in patients with different types of cancer subtypes and different races intensify the importance of using these molecules as biomarkers for disease diagnosis. Therefore, the aim of this study was to investigate the expression of GAS5, NEAT1 and SRA lncRNAs in cancer specimens with cancer and in healthy individuals. In this study, from the tumor tissue of 22 patients with breast cancer and also 22 samples of healthy tissue from individuals under the direct supervision of a pathologist and according to clinical signs and laboratory findings were collected from hospitals in Isfahan. After RNA extraction from tumor and normal tissue, cDNA was fabricated according to RT-qPCR method. The lncRNA expression level of GAS5, NEAT1 and SRA genes was calculated by ∆∆CT method. The expression pattern was analyzed using Rest 2009 software as well as SPSS version 16. Real Time Reverse transcription-PCR results showed that the mean relative gene expression in tumor samples was significantly lower for GAS5 and NEAT1 lncRNAs than normal samples. But no expression change was observed for lncRNA SRA.
Leyla Najafi; Mohammad Fazilati; Hossein Salavati
Abstract
C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various ...
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C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes.
Shahrzad Fouladi; Reza Haji Hosseini; Masoud Torkzadeh-Mahani; Hooshang Hamidian
Abstract
Urate oxidase or uricase (UOX) (EC 1.7.3.3) is an globular tetramer oxidoreductase enzyme which lacks cofactor. Much research on this enzyme has been done so far due to its biomedical applications as a therapeutic and diagnostic agent. Urate oxidase catalyzes uric acid degradation and produces allantoin. ...
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Urate oxidase or uricase (UOX) (EC 1.7.3.3) is an globular tetramer oxidoreductase enzyme which lacks cofactor. Much research on this enzyme has been done so far due to its biomedical applications as a therapeutic and diagnostic agent. Urate oxidase catalyzes uric acid degradation and produces allantoin. An imbalance in the excretion of uric acid causes the hyperuricemia. Part of a clinically important diagnostic kit used to measure the concentration of uric acid in the blood is the enzyme uricase. In this research, efforts are made to investigate the effect of triethylammonium maleate (TEAM) ionic liquid on the function of urate oxidase (UOX). Ionic liquids are salts with important properties like high thermal stability, high solvating capacity and high polarity. These salts have been considered in biochemical and biomedical fields. They can also be utilized as a stabilizer for long-term protein storage and they can extend the shelf life of some proteins such as therapeutic or industrial proteins. In this study, we treated the enzyme in different concentrations of triethylammonium maleate ionic liquid. The volume percentages of TEAM in the solvent phase are 1%, 2%, 5%, 10% and 15%. The results indicate that TEAM has a concentration-dependent effect on the activity of UOX enzyme. The use of 5% TEAM ionic liquid increased the enzymatic activity in comparison to untreated enzyme. We concluded that this ionic liquid was able to alter the structure of the uricase in a way that increased the activity and improve its catalytic efficiency of uricase.
Abbas Ashoori; HOSSEIN VARASTEH MORADI; Farhad Hosseini Tayefeh
Abstract
Anzali International Wetland is one of the most important bird sites in Iran and the region, but so far no comprehensive study has been conducted to determine the diversity and status of its birds. In this study, all parts of the wetland were monitored monthly from January 2014 to December 2015 with ...
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Anzali International Wetland is one of the most important bird sites in Iran and the region, but so far no comprehensive study has been conducted to determine the diversity and status of its birds. In this study, all parts of the wetland were monitored monthly from January 2014 to December 2015 with the aim of identifying and surveying the status of the birds in the wetland. A total of 243 bird species including 112 waterbirds and 131 terrestrial species were identified. The highest number of species was recorded in April and the lowest number in June, and more than 70% of the birds in the wetland were passage and wintering migrants. The highest number of birds was counted in January (134,167 individuals) and the lowest number in May (6,979 individuals). Twenty-six percent of the birds were globally threatened, near-threatened and nationally protected in Iran, and nine species of were named: Sociable Lapwing in the critically endangered, White-headed Duck, Saker Falcon and Steppe Eagle in the endangered, and Common Pochard, Horned Grebe, Greater Spotted Eagle, Eastern Imperial Eagle and European Turtle Dove in the vulnerable category. Breeding of Ferruginous Duck, Savi’s Warbler, Little Crake and Little Tern was recorded for the first time in this wetland and more than one percent of the global population of Whiskered Tern, Teal, Mallard, Gadwall, Whooper Swan, Pygmy Cormorant, Great cormorant and Dalmatian Pelican occur in this wetland. This study shows that Anzali wetland, despite being included in the Ramsar Convention’s Montreux Record of listed sites experiencing change in ecological character, is still one of the most important sites for birds, especially waterbirds, and still satisfies all the waterbird-related criteria of the Ramsar Convention. Therefore, maintaining the quality and extent of the wetland and its valuable habitats is essential.
Masumeh Akbari; Mahdi Khodaei; Alireza Shayestehfar; Seyyed Mehdi Talebi
Abstract
Tick transmit infective agents to humans and animals, and carriers of parasitic and viral diseases such as the Crimean Congo bleeding fever. The purpose of this study was to identify valuable morphobiometric characters for differentiation of Ixodida order using morphobiometric methods. For this purpose, ...
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Tick transmit infective agents to humans and animals, and carriers of parasitic and viral diseases such as the Crimean Congo bleeding fever. The purpose of this study was to identify valuable morphobiometric characters for differentiation of Ixodida order using morphobiometric methods. For this purpose, a total of 186 ticks were identified from the Argasidae family belonging to the two genera of Argas and Ornithodoros, including the species Argas persicus, A. reflexus and Ornithodoros Lahorensis. 413 ticks are from the Ixodidae family, which belongs to 3 genera and 5 species including Rhipicephalus sanguineus, R. turanicus, Hyalomma anatolicum, H. asiaticum, Haemaphysalis sulcata and were identified using several valid identification keys. The study of species diversity and frequency of cattle, sheep and goats in all age groups and both sexes were done in ruminant including cattle, sheep and goats. To this end, 20 stations were selected in the Mashhad Meyghan area and sampling was carried out monthly and during one year from April to March 2017. A total of 18 morphobiometric parameters were determined using calibrated stereomicroscope. The data were analyzed by PCA method and analyzed using SPSS software (version 16), and analyzed by UPGMA, PCO and PCA methods using MVSP software, then shrubs and the corresponding charts were drawn. In PCA analysis, a total of 4 components of morphobiometric traits were obtained, which explained 91% of the total variance. One-way ANOVA showed a significant difference between between most of the traits.
Fereshteh Keshavarzian; Abdolhassan Doulah; Maryam Rafieirad
Abstract
Parkinson is a chronic and widespread neurodegenerative disorder. In this study, the effects of swimming exercise and oleuropein supplementation on oxidative stress of brain tissue in experimental model of Parkinson's disease in rat were investigated. In this experimental study, 40 adult male Wistar ...
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Parkinson is a chronic and widespread neurodegenerative disorder. In this study, the effects of swimming exercise and oleuropein supplementation on oxidative stress of brain tissue in experimental model of Parkinson's disease in rat were investigated. In this experimental study, 40 adult male Wistar rats were selected and divided into five groups including: control group, Parkinson's group, swimming exercise, oleuropein consumption, swimming exercise with oleuropein consumption. The groups were forced to swim for 4 weeks (five sessions per week for 30 minutes each session) and groups 4 and 5 received 20 mg of oleuropein daily per kilogram of body weight orally for 4 weeks. Serum levels of the variables were evaluated using the thiobarbituric acid method. Data were analyzed using one-way ANOVA and Tukey post hoc tests (p≥0.05). In this study, oleuropein-Parkinson, swimming exercise-Parkinson, and swimming exercise with oleuropein-Parkinson consumption significantly decreased Malondialdehyde levels, significantly increased glutathione peroxidase activity and thiol compared to Parkinson's group (P<0/001). Swimming exercise with oleuropein consumption can improve antioxidant defense and decrease lipid peroxidation. Therefore, this interactive effect may necessitate more therapeutic and preventive treatment to prevent oxidative stress-related diseases.
Arash Jouladeh Roudbar; Hamid Farahmand; Amirreza Abed Elmdoust; Bagher Mojazi amiri; Soheil Eagderi
Abstract
Luciobarbus is one of the most important and economical genus of the Cyprinidae family, most of them are similar in appearance, making it difficult to identify species. In the present study, 519 stations in seven basins, including: Caspian, Namak, Urmia, Hari, Tigris, Persis and Hormuz basins were sampled ...
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Luciobarbus is one of the most important and economical genus of the Cyprinidae family, most of them are similar in appearance, making it difficult to identify species. In the present study, 519 stations in seven basins, including: Caspian, Namak, Urmia, Hari, Tigris, Persis and Hormuz basins were sampled using nets, electro fisher and jigging, during the years 2011-2011. In total, the presence of nine species including L. barbulus, L. brachycephalus, L. capito, L. conocephalus, L. esocinus, L. kersin, L. mursa, L. subquincunciatus and L. xanthopterus was confirmed in Iran's inland waters. For the approved members of this genus in Iran, the identification key were presented and their distribution map were prepared. Also, after examining the conservation status of the members of this genus, it was suggested that a well-codified, principled and scientific program be considered to identify populations, suitable places for reproduction and release of larvae from reproduction in captivity.
