Evaluation of Expression of Long ‎Non-Coding RNAs of GAS5, ‎NEAT1 and SRA Genes in ‎Patients with Breast Cancer and ‎Healthy People

Saeidian, Shahriar; Baghaei Far, Zahra; Parvanak, Zivar; Fathi, Mokhtar

doi:10.30473/eab.2021.60350.1846

Document Type : Article

Authors

¹ Assistan Professor of Biochemistry, Payame Noor university, ‎Iran

² Assistant Professor of Cell and Developmental Biology, ‎Payame Noor University, Iran ‎

³ M.A. of Biochemistry, Payame Noor University, Iran

⁴ Assistant Professor of Animal Science, Agriculture Collage, ‎Payame Noor University, Iran ‎

https://doi.org/10.30473/eab.2021.60350.1846

Abstract

Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential of these molecules as biomarkers for disease diagnosis. Also, changes in the expression of lncRNAs in patients with different types of cancer subtypes and different races intensify the importance of using these molecules as biomarkers for disease diagnosis. Therefore, the aim of this study was to investigate the expression of GAS5, NEAT1 and SRA lncRNAs in cancer specimens with cancer and in healthy individuals. In this study, from the tumor tissue of 22 patients with breast cancer and also 22 samples of healthy tissue from individuals under the direct supervision of a pathologist and according to clinical signs and laboratory findings were collected from hospitals in Isfahan. After RNA extraction from tumor and normal tissue, cDNA was fabricated according to RT-qPCR method. The lncRNA expression level of GAS5, NEAT1 and SRA genes was calculated by ∆∆CT method. The expression pattern was analyzed using Rest 2009 software as well as SPSS version 16. Real Time Reverse transcription-PCR results showed that the mean relative gene expression in tumor samples was significantly lower for GAS5 and NEAT1 lncRNAs than normal samples. But no expression change was observed for lncRNA SRA.

Keywords

20.1001.1.23222387.1400.10.2.1.3

References

Balazs, I.; Phillip, T. (2015). Breast Cancer Survivorship: A Comprehensive Review of Long-Term Medical Issues and Lifestyle Recommendations. The Permanente Journal; 19(2): 48-79.

Berteaux, N.; Lottin, S.; Monte, D.; Pinte, S.; Quatannens, B. (2005). H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1The Journal of Biological Chemistry; 280:29625-29636.

Choudhry, H.; Schodel, J.; Albukhari, A.; Oikonomopoulos, S.; Haider, S.; Moralli, D. (2014). Unlocking the complexity of hypoxia non-coding transcriptome landscape of breast cancer. BMC Genom; 15(2): 30.

Davies, B.R.; Auersperg, N.; Worsley, S.D.; Ponder, B.A. (1998). Transfection of rat ovarian surface epithelium with erb-B2/neu induces transformed phenotypes in vitro and the tumorigenic phenotype in vivo. The American Journal of Pathology; 152(1): 297-306.

Ebrahimi, M.; Vahdaninia, M.; Montazeri, A. (2002). Risk factors for breast cancer in Iran: a case-control study. Breast Cancer Research; 4(5): R10.

Guttman, M.; Amit, I.; Garber, M.; French, C.; Lin, M.F. (2009). Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature; 458: 223-227.

Gibb, E.A.; Brown, C.J.; Lam, W.L. (2011). The functional role of long non-coding RNA in human carcinomas. Molecular Cancer; 10: 38.

Karami, F.; Mehdipour, P. (2013). A Comprehensive Focus on Global Spectrum of BRCA1 and BRCA2 Mutations in Breast Cancer. BioMed Research International. 928562.

Lanz, R.B.; Chua, S.S. (2003). Barron imulates proliferation as well as apoptosis in vivo. Molecular and Cellular Biology; 23: 7163-76.

Leygue, E. (2007). Steroid receptor RNA activator (SRA1): unusual bifaceted gene products with suspected relevance to breast cancer. Nuclal Receptor Signal 5: e006.

Meng, J.; Li, P.; Zhang, Q.; Yang, Z.; Fu., S. (2014). A four-long non-coding RNA signature in predicting breast cancer survival. The Journal of Experimental & Clinical Cancer Research; 33(84): 1-10.

Mourtada-Maarabouni, M.; Pickard, M.R.; Hedge, V.L.; Farzaneh, F.; Williams, G.T. (2009). GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer. Oncogene; 28: 195-208.

Perander, M. (2014). The long non-coding RNA NEAT1 is upregulated during epithelial-mesenchymal transition and is abnormally expressed in breast cancer. RNA; 20: 1844-184.

Xin, Y.U.; Zheng, L.I. (2015). Long non‑coding RNA growth arrest‑specific transcript 5 in tumor biology (Review). Oncology Letters; 10(4): 1953-1958.

Zhang, Z.; Zhu, Z.; Watabe, K.; Zhang, X.; Bai, C.; Xu, M. (2013). Negative regulation of lncRNA GAS5 by miR-21. Cell Death & Differentiation; 20(11): 1558-1568.

Experimental animal Biology

Evaluation of Expression of Long ‎Non-Coding RNAs of GAS5, ‎NEAT1 and SRA Genes in ‎Patients with Breast Cancer and ‎Healthy People

References

References

Volume 10, Issue 2 - Serial Number 38
November 2021
Pages 11-19

Evaluation of Expression of Long ‎Non-Coding RNAs of GAS5, ‎NEAT1 and SRA Genes in ‎Patients with Breast Cancer and ‎Healthy People

References

References

Volume 10, Issue 2 - Serial Number 38November 2021Pages 11-19

Volume 10, Issue 2 - Serial Number 38
November 2021
Pages 11-19