Development
Nasim Hayati; Amirhosein Fazlali; Gholamreza Kaka; Kazem Parivar
Abstract
Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were ...
Read More
Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were divided into 4 groups of 7 Healthy rats, sciatic nerve cut rats without therapeutic intervention, rats with umbilical cord blood cells on PLGA membrane scaffold at the nerve cut site, rats were treated with umbilical cord blood cell injection at the injury site. The degree of recovery was evaluated by the sensorimotor activity of the sciatic nerve, electrophysiological studies.
Findings: motor evaluation of the sciatic nerve, the control group was not observed to return to the normal state in the eighth week, the cell therapy group was repaired on the PLGA membrane scaffold in the eighth week. The level of AMP in the 8th week after recovery of the cell therapy group with a gentle slope is a sign of the healing process of the cell therapy group. Counting the number of nerve fibers in a surface equal to 1000 square meters, the number of nerve fibers in the cell therapy groups increased after the eighth week of repair, compared to the control group and the PLGA membrane group. At the end of the eighth week, the sensory activity index of the sciatic nerve (Hot Plate test), the healing process of the cell therapy group on the PLGA membrane scaffold was more evident than the other groups.
Conclusion: Umbilical cord blood cell transplantation causes sciatic nerve repair and PLGA membrane scaffold with cord blood cells accelerates sciatic nerve repair.
Development
Fatemeh Razeghi; Marjan Nouri; Fattah Sotoodehnejadnematalahi; Ehsan Ehsani; Kazem Parivar
Abstract
Kidney diseases are an important medical problem worldwide. Since there are limited treatment options for damaged kidneys, stem cell therapy has become an alternative treatment. The aim of present study is to investigate effect of culture medium obtained from mesenchymal stem cells (MSCs-CM) of newborn ...
Read More
Kidney diseases are an important medical problem worldwide. Since there are limited treatment options for damaged kidneys, stem cell therapy has become an alternative treatment. The aim of present study is to investigate effect of culture medium obtained from mesenchymal stem cells (MSCs-CM) of newborn mice kidneys in percentages of 10, 30 and 50 on differentiation of embryonic stem cells towards kidney epithelial cells. Mesenchymal stem cells were isolated from kidneys of newborn mice, passaged and propagated. Determination of the identity of cells was done using flow cytometry and checking the expression of surface markers CD105, CD29, CD90. In third passage of extracted cells, supernatant culture medium was collected, hESC were cultured and multiplied in the complete culture medium of cells and the differentiation of hES cells into progenitor cells was investigated. The expression of PAX2, ZO1 and CK18 genes was investigated using RT-PCR, expression of CD133, CD24 and CD44 surface markers was investigated using flow cytometry. Flow cytometry results confirmed the mesenchymal nature of the cells. The results of differentiation of hESCs showed that expression of PAX2, ZO1 and CK18 genes increased significantly (p<0.05) in the groups containing supernatant. The results of flow cytometry show an increase in expression of CD133 and CD24 markers in groups containing CM and the expression of CD44 marker in the group containing 50% CM, compared to control group. In general, results showed supernatant culture process of cells has a positive effect on inducing differentiation of human embryonic stem cells into kidney progenitor cells.
Kazem Parivar; Nasim Hayati Roodbari; Alireza Badiei; Roghiyeh Taghizadeh Hajiagha
Volume 4, Issue 1 , September 2015, , Pages 1-10
Abstract
Abstract Increase in the use of nano zinc oxide in medicine, manufacturing cosmetic products and research, have raised the questions about their toxicity. Zinc has direct role in growth, maturation and ovulation through influence on production of estradiol and progesterone. In this study the effects ...
Read More
Abstract Increase in the use of nano zinc oxide in medicine, manufacturing cosmetic products and research, have raised the questions about their toxicity. Zinc has direct role in growth, maturation and ovulation through influence on production of estradiol and progesterone. In this study the effects of nano zinc oxide on oogenesis of adult NMRI mouse strain have been investigated. The adult female mice were randomly divided into six groups, including control, sham and four experimental groups. Experimental groups were injected nano-ZnO (50-100-150-300 mg/kgbw) Intraperito-neally, respectively for 3 days. After 7 day, histological changes of ovaries were studied. Data indicated increase of numbers of Graafian follicles and corpora lutea in 100 and 150 mg/kg bw doses. Increase in estradiol and progestron levels with increase on dose was observed. Data indicated increase of numbers of atretic follicles with decreased body weight in higher doses. Results indicated oogenesis under 100 and 150 doses nano-Zno has improved with enhancing the number of Graafian follicles and corpora lutea, and decrease of body weight and increase of number of atretic follicles under higher doses. The results also indicated that nano-Zno has toxic effects on reproductive organs in female animal.
N Hayati Roodbari; K parivar; S Rezaee; A.R. Badiee
Volume 3, Issue 1 , January 2015, , Pages 27-38
Abstract
According to increasing use of nano materials and our daily exposure to them, it is required to investigate their effects on genital organs. Upon this purpose, in this resserch the effect of nano titanium dioxide on the spermatogenesis were studied. The effect of titanium dioxide with particle ...
Read More
According to increasing use of nano materials and our daily exposure to them, it is required to investigate their effects on genital organs. Upon this purpose, in this resserch the effect of nano titanium dioxide on the spermatogenesis were studied. The effect of titanium dioxide with particle size of 10 nm on spermatogenesis of adult male NMRI mice strain in “in vivo” conditions was studied. Three groups: control (no treatment), sham (treated with distilled water), experimental (treatment with doses of 6, 30, 150, 300 mg / kg of nano titanium dioxide 10nm) were investigated. Intraperitoneal injection was applied for 5 consecutive days. 2 weeks after the last injection, mice were anesthetized and their testes and epididymis were removed. After fixing and preparing slides, they were examined. In this study, mouse weight, number of tubes in the testes and epididymis, tunica albuginea thickness, number of Leydig cells, fibroblasts, Sertoli and sperm numbers at three significant levels (p
