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Behavioral and electrophysiological study of the healing process ‎of severed sciatic nerve in male Wistar rats with the help of ‎umbilical cord stromal cells cultured on Plga nanofiber scaffolds

Document Type : Article

Authors

1 Department of Cell Biology, Faculty ‎of Basic Sciences, Islamic Azad ‎University, Research Branch, Tehran, ‎Iran

2 Baqiyatallah University of Medical ‎Sciences, Tehran, Iran

3 ‎1Department of Cell Biology, Faculty ‎of Basic Sciences, Islamic Azad ‎University, Research Branch, Tehran, ‎Iran

10.30473/eab.2024.71486.1954

Abstract

Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were divided into 4 groups of 7 Healthy rats, sciatic nerve cut rats without therapeutic intervention, rats with umbilical cord blood cells on PLGA membrane scaffold at the nerve cut site, rats were treated with umbilical cord blood cell injection at the injury site. The degree of recovery was evaluated by the sensorimotor activity of the sciatic nerve, electrophysiological studies.
Findings: motor evaluation of the sciatic nerve, the control group was not observed to return to the normal state in the eighth week, the cell therapy group was repaired on the PLGA membrane scaffold in the eighth week. The level of AMP in the 8th week after recovery of the cell therapy group with a gentle slope is a sign of the healing process of the cell therapy group. Counting the number of nerve fibers in a surface equal to 1000 square meters, the number of nerve fibers in the cell therapy groups increased after the eighth week of repair, compared to the control group and the PLGA membrane group. At the end of the eighth week, the sensory activity index of the sciatic nerve (Hot Plate test), the healing process of the cell therapy group on the PLGA membrane scaffold was more evident than the other groups.
Conclusion: Umbilical cord blood cell transplantation causes sciatic nerve repair and PLGA membrane scaffold with cord blood cells accelerates sciatic nerve repair.

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References
Azadeh Tajik et al. (2014). Effect of non-autologous adipose-derived stem cells transplantation and nerve growth factor on the repair of crushed sciatic nerve in rats. Journal of Birjand University of Medical Sciences. 20 (4), 357-365.
Bateman, E. A., Pripotnev, S., Larocerie‐Salgado, J., Ross, D. C., & Miller, T. A. (2024). Assessment, management, and rehabilitation of traumatic peripheral nerve injuries for non‐surgeons. Muscle & Nerve.
Bojanic, C., To, K., Zhang, B., Mak, C., & Khan, W. S. (2020). Human umbilical cord derived mesenchymal stem cells in peripheral nerve regeneration. World journal of stem cells, 12(4), 288.
Cao, F. J., & Feng, S. Q. (2009). Human umbilical cord mesenchymal stem cells and the treatment of spinal cord injury. Chinese medical journal, 122(02), 225-231.
Cattin, A. L., & Lloyd, A. C. (2016). The multicellular complexity of peripheral nerve regeneration. Current opinion in neurobiology, 39, 38-46.
Cho, Y. H., & Seo, T. B. (2022). The timing point of exercise intervention regulates neuropathic pain-related molecules in the ipsilateral dorsal root ganglion neurons after sciatic nerve injury. Journal of exercise rehabilitation, 18(5), 286.
Dadon-Nachum, M., Melamed, E., & Offen, D. (2011). Stem cells treatment for sciatic nerve injury. Expert opinion on biological therapy, 11(12), 1591-1597.
Galhom, R. A., Abd El Raouf, H. H. H., & Ali, M. H. M. (2018). Role of bone marrow derived mesenchymal stromal cells and Schwann-like cells transplantation on spinal cord injury in adult male albino rats. Biomedicine & Pharmacotherapy, 108, 1365-1375.
Gordon, T. (2016). Electrical stimulation to enhance axon regeneration after peripheral nerve injuries in animal models and humans. Neurotherapeutics, 13(2), 295-310.
Guo, W., Imai, S., Yang, J. L., Zou, S., Li, H., Xu, H., ... & Ren, K. (2018). NF-KappaB pathway is involved in bone marrow stromal cell-produced pain relief. Frontiers in Integrative Neuroscience, 12, 49.
Ishii, T., Sakai, D., Schol, J., Nakai, T., Suyama, K., & Watanabe, M. (2017). Sciatic nerve regeneration by transplantation of in vitro differentiated nucleus pulposus progenitor cells. Regenerative Medicine, 12(4), 365-376.
Joyce, N. C., Harris, D. L., Markov, V., Zhang, Z., & Saitta, B. (2012). Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium. Molecular Vision, 18, 547.
Kerosuo, L., Nie, S., Bajpai, R., & Bronner, M. E. (2015). Crestospheres: long-term maintenance of multipotent, premigratory neural crest stem cells. Stem cell reports, 5(4), 499-507.
Li, C., Luo, Y., & Li, S. (2024). The roles of neural stem cells in myelin regeneration and repair therapy after spinal cord injury. Stem Cell Research & Therapy, 15(1), 204.
Lotfinia, M., Lak, S., Ghahhari, N. M., Johari, B., Maghsood, F., Parsania, S., ... & Kadivar, M. (2017). Hypoxia pre-conditioned embryonic mesenchymal stem cell secretome reduces IL-10 production by peripheral blood mononuclear cells. Iranian biomedical journal, 21(1), 24.
Ma, Y., Dong, L., Zhou, D., Li, L., Zhang, W., Zhen, Y., ... & Wang, X. (2019). Extracellular vesicles from human umbilical cord mesenchymal stem cells improve nerve regeneration after sciatic nerve transection in rats. Journal of cellular and molecular medicine, 23(4), 2822-2835.
Mehrasa, M., Asadollahi, M. A., Ghaedi, K., Salehi, H., & Arpanaei, A. (2015). Electrospun aligned PLGA and PLGA/gelatin nanofibers embedded with silica nanoparticles for tissue engineering. International journal of biological macromolecules, 79, 687-695.
Moazami Goudarzi, M., Azarnia, M., Kaka, G., Sadraii, S. H., & Khatibi Aghda, A. (2013). Study of bone marrow stromal cells, nerve growth factor, and marginal on nerve regeneration in rat crushed sciatic nerve. Journal of Mazandaran University of Medical Sciences, 23(102), 96-105.
Mobasher, M., Aramesh, K., Ale Davous, S.J., Ashraf Gangoie, N., Divsalar, K., Philips, C.J.C., & Larijani Mohamad Bagher, A. (2008). Proposing A Nathional Ethical Framwork for animal reserch in Iran. Iranian Journal of Public Health, 37(1) (supplementary isuue on bioechics), 39-46.
Molina-Gonzalez, I., Holloway, R. K., Jiwaji, Z., Dando, O., Kent, S. A., Emelianova, K., ... & Miron, V. E. (2023). Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration. Nature Communications, 14(1), 3372.
Paskal, A. M., Paskal, W., Pietruski, P., Kusmierczyk, Z., Jankowska-Steifer, E., Andrychowski, J., & Wlodarski, P. K. (2020). Neuroregenerative effects of polyethylene glycol and FK-506 in a rat model of sciatic nerve injury. Journal of Plastic, Reconstructive & Aesthetic Surgery, 73(2), 222-230.
Sulaiman, W., & Gordon, T. (2013). Neurobiology of peripheral nerve injury, regeneration, and functional recovery: from bench top research to bedside application. Ochsner Journal, 13(1), 100-108.
Viswanathan, S., Ciccocioppo, R., Galipeau, J., Krampera, M., Le Blanc, K., Martin, I., ... & Ashford, P. (2021). Consensus International Council for Commonality in Blood Banking Automation–International Society for Cell & Gene Therapy statement on standard nomenclature abbreviations for the tissue of origin of mesenchymal stromal cells. Cytotherapy, 23(12), 1060-1063.
Wakao, S., Hayashi, T., Kitada, M., Kohama, M., Matsue, D., Teramoto, N., ... & Dezawa, M. (2010). Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration. Experimental neurology, 223(2), 537-547.
Wang, Z., & Jia, X. (2023). Treating peripheral nerve injury-induced spinal cord degeneration and neuropathic pain with peripherally administrated stem cells. Neural Regeneration Research, 18(3), 537-538.
Xu, X. D., Lin, L., Qiu, Y. B., Zeng, B. W., Chen, Y., Liu, J. L., ... & Zhang, L. C. (2023). Ultrasonic visualization technique for anatomical and functional analyses of the sciatic nerve in rats. Frontiers in Neuroscience, 17, 1187669.
Yu, J. I., Cho, Y. H., Seo, T. B., & Kim, Y. P. (2023). Effect of combined intervention of exercise and autologous bone marrow stromal cell transplantation on neurotrophic factors and pain-related cascades over time after sciatic nerve injury. Journal of exercise rehabilitation, 19(1), 19.
Zainul, Z., Ma, B., Koka, M., Wilkerson, J. L., Ortiz, Y. T., Kerosuo, L., & Chandran, V. (2022). Novel roles of phentolamine in protecting axon myelination, muscle atrophy, and functional recovery following nerve injury. Scientific Reports, 12(1), 3344.
Zhou, G., Chang, W., Zhou, X., Chen, Y., Dai, F., Anwar, A., & Yu, X. (2020). Nanofibrous nerve conduits with nerve growth factors and bone marrow stromal cells pre-cultured in bioreactors for peripheral nerve regeneration. ACS applied materials & interfaces, 12(14), 16168-16177.
Zolfagari, D., Kaka, G., Sadraee, S. S. H., & Herfehdoost, G. (2014). Characterization of bone marrow stromal cell growth on substrate PLGA nanofibers. Journal of Mazandaran University of Medical Sciences, 24(117), 65-73.
Zou, X., Dong, Y., Alhaskawi, A., Zhou, H., Ezzi, S. H. A., Kota, V. G., ... & Wang, C. (2024). Techniques and graft materials for repairing peripheral nerve defects. Frontiers in Neurology, 14, 1307883.
Experimental animal Biology
Volume 13, Issue 1 - Serial Number 49
No.1
September 2024
Pages 69-80
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