Zahra Khodabandeh; Sanaz Alaee; Mahintaj Dara; Maryam Davari; Azizollah Bakhtari
Abstract
Sodium polyacrylate is a material with a high potential for water and moisture absorption. In this study, the effects of this material on the rate of embryo development and the expression of apoptosis-related and antioxidants genes in the blastocyst was evaluated. Adult female mice were superovulated ...
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Sodium polyacrylate is a material with a high potential for water and moisture absorption. In this study, the effects of this material on the rate of embryo development and the expression of apoptosis-related and antioxidants genes in the blastocyst was evaluated. Adult female mice were superovulated by injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) and after placing beside adult male mice, zygotes were harvested from oviducts and transferred into media containing 0, 5, 25, and 50 μg/ml sodium polyacrylate. Zygotes were cultured towards the blastocyst stage and the rate of embryo development was assessed. Expression of intended genes were evaluated by real time RT-PCR. One-way analysis of variance (ANOVA) and Duncan's post hoc toast were used to determine the differences between the means of the groups. The rate of blastocysts was significantly lower in 50 μg/ml compared to the control group (P<0.05). The expression of Bcl-2 increased significantly in 5 μg/ml in comparison to the control group and decreased significantly in 50 μg/ml compared to 5 and 25 μg/ml (P<0.05). The expression of Bax/Bcl-2 and Caspase-3 in 50 μg/ml increased significantly in comparison to 0, 5, and 25 μg/ml (P<0.05). Therefore, the high concentration of sodium polyacrylate has an adverse effect on the embryo through apoptosis system.
Naeimeh Dehghani; Mehdi Dianatpour; Seyed Ebrahim Hosseini; Zahra Khodabandeh; Hamed Daneshpazhouh
Abstract
As a stabilizing agent, docetaxel can potentially reduce the damage to the oocyte cytoskeleton during vitrification. The aim of the present study was to investigate the effect of docetaxel on the survival rate and in vitro fertilization of oocytes after vitrification. NMRI mice (8-10 weeks old) were ...
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As a stabilizing agent, docetaxel can potentially reduce the damage to the oocyte cytoskeleton during vitrification. The aim of the present study was to investigate the effect of docetaxel on the survival rate and in vitro fertilization of oocytes after vitrification. NMRI mice (8-10 weeks old) were superovulated by injecting PMSG and HCG. Oocytes are surrounded by cumulus and corona cells and must be denuded by 0.1% hyaluronidase enzyme. The oocytes were then divided into 5 experimental groups including control, docetaxel, docetaxel+vitrification solution; docetaxel+ vitrification and vitrification. Mature oocytes were vitrified in ethylene glycol and dimethyl sulfoxide solutions at 15% concentration and 0.5 M sucrose. After thawing, their survival and fertilization rates were assessed up to the two-cell stage. Staining of the microtubules in the oocytes was performed with alpha-tubulin antibody. The fertilization rate of each group showed a significant decrease compared to the control group (P=0.001). The rate of formation of 2-cell embryos in both vitrified groups (docetaxel+ vitrified and vitrified vitrified) was significantly lower than non-vitrified (control (P=0.001) and docetaxel ((P=0.004)). The results showed that survival and fertilization rates in pre-incubated groups with docetaxel were higher than non-incubated groups, so docetaxel could improve reproductive techniques by reducing the damage to the oocyte cytoskeleton.
