Evaluation of the effect of docetaxel on ‎cryotop-vitrification of mouse oocytes

Dehghani, Naeimeh; Dianatpour, Mehdi; Hosseini, Seyed Ebrahim; Khodabandeh, Zahra; Daneshpazhouh, Hamed

doi:10.30473/eab.2020.45820.1705

Document Type : Article

Authors

¹ Assistant Professor, Department of Biology, Payame Noor Unvierstiy, ‎Tehran, Iran

² Associate Professor, Stem Cells Technology Research Center, ‎Shiraz University of Medical Sciences, Shiraz, Iran

³ Professor, Department of Biology, College of Science, Shiraz ‎Branch, Islamic Azad University, Shiraz, Iran ‎

⁴ Assistant professor, Stem Cells Technology Research Center, ‎Shiraz University of Medical Sciences, Shiraz, Iran

⁵ Ph.D., Department of Biology, Payam Noor University, Tehran, ‎Iran

https://doi.org/10.30473/eab.2020.45820.1705

Abstract

As a stabilizing agent, docetaxel can potentially reduce the damage to the oocyte cytoskeleton during vitrification. The aim of the present study was to investigate the effect of docetaxel on the survival rate and in vitro fertilization of oocytes after vitrification. NMRI mice (8-10 weeks old) were superovulated by injecting PMSG and HCG. Oocytes are surrounded by cumulus and corona cells and must be denuded by 0.1% hyaluronidase enzyme. The oocytes were then divided into 5 experimental groups including control, docetaxel, docetaxel+vitrification solution; docetaxel+ vitrification and vitrification. Mature oocytes were vitrified in ethylene glycol and dimethyl sulfoxide solutions at 15% concentration and 0.5 M sucrose. After thawing, their survival and fertilization rates were assessed up to the two-cell stage. Staining of the microtubules in the oocytes was performed with alpha-tubulin antibody. The fertilization rate of each group showed a significant decrease compared to the control group (P=0.001). The rate of formation of 2-cell embryos in both vitrified groups (docetaxel+ vitrified and vitrified vitrified) was significantly lower than non-vitrified (control (P=0.001) and docetaxel ((P=0.004)). The results showed that survival and fertilization rates in pre-incubated groups with docetaxel were higher than non-incubated groups, so docetaxel could improve reproductive techniques by reducing the damage to the oocyte cytoskeleton.

Keywords

20.1001.1.23222387.1399.9.2.6.1

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Experimental animal Biology

Evaluation of the effect of docetaxel on ‎cryotop-vitrification of mouse oocytes

References

References

Volume 9, Issue 2 - Serial Number 34
November 2020
Pages 73-81

Evaluation of the effect of docetaxel on ‎cryotop-vitrification of mouse oocytes

References

References

Volume 9, Issue 2 - Serial Number 34November 2020Pages 73-81

Volume 9, Issue 2 - Serial Number 34
November 2020
Pages 73-81