Veterinary
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment ...
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The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment containing 5 pen and 15 birds on each pen. Diets included 0, 1 or 1.5gr of probiotic BIOSAF/kg diet and considered as control, treatment1 and treatment 2, respectively. On day 20 and 35 of age, birds were injected (i.m) with 0.1 mg of 0.5% sheep red blood cells (SRBC). Antibody production against SRBC was measured after 7 days of inoculation. On the day 10 of age, the chicks were vaccinated against Newcastle disease virus (NDV Generally, usingprobiotic BIOSAF per kg diet has efficient effect on growth performance of male Ross chekien particularly, when supplementation is done in starter period. Also, supplementation probiotic BIOSAF has positive effect on immune response to Newcastle and SRBC. However, 1gr/kg supplementation of probiotic BIOSAF is more suitable than 1.5gr/kg diet.
Fisheries
seyedeh abedi; mohammad khalesi; Sohrab Eskandari
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity ...
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This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity factor (TF) was determined for each species. For bioaccumulation trial, the two species exposed to sublethal concentrations (10% of LC50 values) of Cr at separate triplicate treatments for 15 days. The examined tissues from each species were sampled at days 7 and 15 of the experiment. Following chemical digestion, each tissue was analyzed by atomic absorption unit (Model Thermo) to measure chromium concentration. According to the results, Almost all differences in bioaccumulations between the two species and the controls were significant (p < 0.05). Concentration of chromium in common carp was in the form of gills> liver> skin> scales> muscle, and accumulation in catfish was in the form of liver> muscle> skin> gills. Final entire contents of Cr in the catfish's tissues were 3.65 times, than in the carp’s (excluding the scales). In addition, the catfish's muscle amassed 16.15 times greater amounts of Cr.The results of this investigation indicate that the carp's scaled armature, compared to the scaleless skin of the catfish, adsorbs metal toxicants leading to much lesser metals content in the muscle rendering this tissue rather safer for comestible consumption.
Veterinary
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity ...
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The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity (GPX units/ml PCV) and Total Antioxidant Status (TASmmol/L). Thirty six pregnant Holstein dairy cows were assigned into two treatment groups based on their BCS and expected.calving date in t student examination. Treatment groups consisted of 1)glutamine supplementation 100 g/d per cow from 21d before calving until parturition (F), 2) glutamine unsupplementation from 21d before calving until parturition (N). There weren’t any significant differences among treatments in DMI and BCS on d -21,-14 ,-7 and calving day before parturition. There weren’t significant differences in The total antioxidant status (TAS) but plasma glutathione Peroxidase activity (GPX) was significant difference between two group and was greater for (F) group at -7d before calving (57.44 vs 47.94 and p≤0.05 respectively) and -14 (45.87 vs 41.72 and p≤0.04 respectively) before parturition. It seems that supplementation diets with glutamine on the close up period can enhance plasma glutathione Peroxides activity (GPX) and the best level of formaldehyde for protection of glutamine is 1%.
animal biosystematic
iman gowhari; shahrokh pashaei rad
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Based on the published statistics of diseases management center, yearly frequency of scorpion bite in Iran between 2001 to 2003 has been 38415 bite and the rate of death wich is resultant of scorpion bite has been 21 cases during a year. Doubtless scorpion bite is one of our medical problems, but what ...
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Based on the published statistics of diseases management center, yearly frequency of scorpion bite in Iran between 2001 to 2003 has been 38415 bite and the rate of death wich is resultant of scorpion bite has been 21 cases during a year. Doubtless scorpion bite is one of our medical problems, but what is important regarding this matter is a fear that people have about this creature. Unfortunately a precise and right statistcs toward the risk of this creature bite does not exist. Till based on that, it woud be possible to recognize scorpion bite of our society is related to what species and it has what a status among category of death causes and based on that prepre a antivenom monovalan related to every scorpion؛ surely we can not deny this problem in south and west regions of our country specially for hot seasons.In this research first, Ilam province based on geography position, scorpion bitting domain, climate and local researches divided to 14 region of sampling. By use of valid identifying keys such as Navid pour(2008), samples recognizes, frequency of scorpion species in each climate region has showed and their distribution map by use of GIS software has drawn. In Ilam province, among 390 hunted scorpion, 10 species include Hottentotta saulcyi(23/07), Mesobuthus eupeus(22/56), Compsobuthus matthiesseni(13/07), hemiscorpius lepturus(12/3), Orthochirus iranus(7/94), Razianus zarudnyi(4/87), Androctonus crassicaudata(4/1), Buthacus macrocentrus(3/33), Scorpio maurus(2/56), Odontobuthus bidentatus(1/02) scorpion identified.
Animal physiology
Seyed Ebrahim Hosseini; Mozhde Heidari
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Introduction: Due to the increasing use of high intake of saturated fatty acids and their effects on overall physiological structures, effects of unsaturated fatty acids, including omega-3 and omega-6, and the proportion of these two together is essential physiological functions. Therefore, this study ...
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Introduction: Due to the increasing use of high intake of saturated fatty acids and their effects on overall physiological structures, effects of unsaturated fatty acids, including omega-3 and omega-6, and the proportion of these two together is essential physiological functions. Therefore, this study aimed to investigate the interaction of corn and salmon fish’s oil on avoidance learning in mature male rat.Method: In this empirical research work, we used 72 mature male rats weighing from 180-200g were enrolled as the control, sham and experiment groups. The control group was no treated. The sham group received only 1 ml saline, 6 experimental groups of different types received 0.5, 1 and 1.5 ml/kg body weight corn oil, and 0.25, 0.5 and 0.75 ml/kg body weight and 1 group other received doses of 0.5 and 1.5, salmon oil and corn oil respectively, daily for 28 days. For avoidance learning behavior, the rats were trained to shuttle box and 24 hours after training, the rats were tested retrieval. The data were evaluated using ANOVA.Results: The results showed that consumption of corn and salmon’s oil alone enhances learning and taking the time to arrange a 3 to 1 ratio, boost the oil alone is more than the avoidance learning.Discussion and conclusion: Omega-3 and Omega-6 fatty acids the rate 3 to 1, are most effective in promoting avoidance learning, so further investigation can be listed using ratios to enhance learning and prevention of Alzheimer's, it uses .
Animal physiology
mokhtar fathi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied ...
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A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group set as control (healthy) group and reared under normal temperature, second group set as PHS group and reared under cold temperature. At the end of the experiment (day42), 2 chicks from each replicate were randomly selected bled and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. The results showed that hematocrit value, mortality due to ascites, RV/TV index and differential white blood cells count were significantly higher in CT treatment chick (P˂0.05). The concentrations of plasma t4 of CT birds were significantly lower than other group birds at day 21 & 42. it is also, The concentrations of plasma t3 of CT birds were significantly higher than other group birds at day 21(exception day 42) (P˂0.05).
histology
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured ...
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To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured by method Enzymatic, serum levels of lipoproteins were determined by method Precipitation and serum levels of testosterone were measured by method Radio Immuno Assay. For histomorphometric study, the 5 µ sections were made and stained with Hematoxyline-Eosin. Data was statistically analyzed by the one way ANOVA and Pearson correlation tests (p≤0.05). Results showed that serum levels of total cholesterol, triglycerids, VLDL-c and LDL-c had not significant differences between different ages. The serum levels of HDL-c, testosterone, as well as the area of spermatic tubules and testicular interstitium, the diameter of seminiferous tubules, the germinal epithelium height, the number and diameters of the leydig cells; additionally, the number of spermatid cells had significant increase from one year group to two years group and also significant decrease from two years animals to three years group. The serum HDL-c values were significantly correlated with the number of leydig cells as well as serum levels of testosterone. Serum triglycerides values were also inversely correlated with germinal epithelium height. It can be concluded that increased serum levels of HDL-c and triglycerides have respectively a benefit and deleterious effects on the spermatogenic activity and histomorphometric structure of the testis in the male goats of Ilam province.
Fisheries
Elham Abdzadeh; Fatemeh Nazarhaghighi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, ...
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To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor,with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and blood plasma were measured inorganic phosphorous. The results showed that the cortisol and glucose levels on day 5, there was a significant difference between control and Butachlor treatments (P 0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P
Mehrdad Modaresi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
The scientific name of a dark mountain celery is Apium graveolens. This plant contains phytoestrogens and has many effects on the reproductive system. In this study, hydroalcoholic leaf extract on the testicular tissue and spermatogenesis in male mice was investigated. The samples were randomly divided ...
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The scientific name of a dark mountain celery is Apium graveolens. This plant contains phytoestrogens and has many effects on the reproductive system. In this study, hydroalcoholic leaf extract on the testicular tissue and spermatogenesis in male mice was investigated. The samples were randomly divided into 5 groups (three treatment groups , placebo and control) groups, and all samples were stored in the same condition. Apium leaves hydroalcoholic extract in different doses (mg/kg/2day150, 100,50) was injected (IP) into the treated groups for 20 days (one day in between) . The Placebo group were used for injection of saline. The parameters that were evaluated changes , testicular weight and histology of spermatogenesis was compared with control groups. Data were analyzed by SPSS software and statistical tests one way ANOVA and Duncan test.
This study showed dose-dependent negative effect of Apium extract on spermatogenesis and reproductive potential in male rats.
Keywords : Apium graveolens, Mice, spermatogenesis
Medical physiology
Sayeh Bidaran; Sima Nasri
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
Introduction & Objective: Zinc is an essential element for the immune system. This element has different effects on immune system activity. On the other hand different serum Zn levels also alter normal B-cell line and T-cell line functions. The present study was carried out to assess B cell line and ...
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Introduction & Objective: Zinc is an essential element for the immune system. This element has different effects on immune system activity. On the other hand different serum Zn levels also alter normal B-cell line and T-cell line functions. The present study was carried out to assess B cell line and T cell line tolerance to low concentration Zinc Chloride In vitro studyMaterials & Methods: This experimental study was conducted at the Payame Noor University of Tehran in 2010. The B- cell line and T-cell line were exposed to 1, 5, 10, 20, 30, 40, 50, 75, 100,125, 150,175 and 200 µm (λ) of Zinc Chloride followed by incubation at 12, 24, 36, 48, 60 and 72 hrs. Viability of B- cell line (Raji) and T-cell line (molt-4) were evaluated with hemocytometer method and percentage of living cells have been considered as cellular tolerance index to cytotoxic effects of Zinc chloride. The results were analyzed by SPSS software, version 18 using T-test and Kruskal-Wallis testResults: In this study, the results showed Zinc chloride concentrations up to 40 µm (λ) at different incubation time points (12-72 hrs) had no effects on B cell line and T cell line viability( p> 0.05) With 50-200 µm (λ) concentrations, Zinc at different incubation time points(12-72 hrs)
animal biosystematic
Valiallah Khalaji-Pirbalouty
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the ...
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In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the detailed drawings are made using special pens and proper lines. The final illustrations produced with this method can be stored in all known formats and used in the scientific literatures. The digitizer panel, as an alternative option, may be used to schedule the initial drawing and also adding the details.Key words: Animal illustrations, CorelDraw, Digital drawings.In this paper, we propose a simple, fast, and accurate method to make scientific illustrations of animal and specimens. Photos and/or pencil drawings provided via microscope or stereo-microscope equipped with Camera Lucida are scanned. The scanned illustrations are then imported into CorelDraw and the detailed drawings are made using special pens and proper lines. The final illustrations produced with this method can be stored in all known formats and used in the scientific literatures. The digitizer panel, as an alternative option, may be used to schedule the initial drawing and also adding the details.Key words: Animal illustrations, CorelDraw, Digital drawings
Veterinary
Navab Ghobadi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and ...
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The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and second Semenation, amount of pregnancy at first semenation and number of semenation for every pregnancy. In This study 30 Holeshtain dairy cow are used in 2 group of 15(treatment and control group).treatment group recivead 10cc Oxytocin immidiatly after parturition and 5cc prostsglandinF2α,20 to 25 days after parturition.the result statistically using chi sequare and t student test were analyzed with spss.The result show a meaning ful difference(p≥0/05)in uterus contraction ,amount of pregnancy at first Semenation and number of Semenation for every pregnancy between treatment and control group.there is a noticable improvement in percent of pregnancy for Treatment group(47/1percent for treatment group and 23/5percent for control group).The result also shows that injecting oxytocin immidiatly after parturition and prostaglandinf2α 20 to25 days later can hare positive increase in amount of future fertility in dairy cow and also it can decrease economical loss in cow herd. This result can show connection of contraction influence of oxytocin and prostaglandinf2α in myometriom layer of uterus to enjection secrection and early contraction of ovary activity.
animal biosystematic
seyedُ pourrabie; Shahrogh Pashaie Rad; Mahbobeh Hajirostamlo; Hasan Baroghie
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Abstract:
Different species of Syrphid flies are beneficial insects in nature and are important in agriculture. During a faunistic study on syrphid flies (Dip.:Syrphidae) on 2011 to 2012 of Jolfa region (East Azarbaijan province), one Merodon Meigen, 1803 species was collected. They were identified ...
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Abstract:
Different species of Syrphid flies are beneficial insects in nature and are important in agriculture. During a faunistic study on syrphid flies (Dip.:Syrphidae) on 2011 to 2012 of Jolfa region (East Azarbaijan province), one Merodon Meigen, 1803 species was collected. They were identified as Merodon latifemoris Radenkovic, based on morphological characters as a new species from Iran region.
In this study, species was collected, and transferred to the Laboratory of Entomology, in Islamic Azad University of Marand and was studied at the morphological character.
The syrphidae important organs such as legs, wings, thorax, abdomen and genitalia were examined. In this research isolated end of the tergit, and transferred in to KOH 10% for 24-12h and then transferred in to alchole 10%, so added one drope glycrin at study time, and was recognized by prestigious keys. Their figures and a simple diagnostic key were provided for their determination in this region.
Animal physiology
Seyed Ebrahim Hosseini; Elham Rezaei; Davood Mehrabani
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Increasing the levels of blood lipids is the most common factors in diabetes that will exacerbate its complications. PJ (Pomegranate juice) is a nutrient with medical purposes. The present study aimed to investigate the effects of pomegranate juice on levels of triglycerides, cholesterol; LDL and HDL ...
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Increasing the levels of blood lipids is the most common factors in diabetes that will exacerbate its complications. PJ (Pomegranate juice) is a nutrient with medical purposes. The present study aimed to investigate the effects of pomegranate juice on levels of triglycerides, cholesterol; LDL and HDL in healthy and diabetic adult male rats were done. In the study, 90 adult male rats weighing 220-200 g were divided into groups of control, diabetic and non-diabetic experimental groups. Each group includes 9 mice. Experimental groups received respectively 1, 2 and 4 ml of pomegranate juice per rat for 21 days. To create diabetes by Intra-peritoneal injection, streptozotocin 60mg/kg was used. At the end of experiment by the phlebotomizing the animal hearts, serum levels of triglycerides, cholesterol, LDL and HDL were measured. Data were analyzed by ANOVA and LSD tests. The results showed that pomegranate juice in doses of 2 and 4 ml caused a significant reduction in triglyceride levels, respectively, in the P
animal biosystematic
azita farashi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Carnivores play great ecological roles in ecosystems because of placing on top of the food pyramid. Food habits of large carnivores are associated with important ecological features such as spatial pattern, movement, habitat selection, social structure, geographical distribution and reproductive success. ...
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Carnivores play great ecological roles in ecosystems because of placing on top of the food pyramid. Food habits of large carnivores are associated with important ecological features such as spatial pattern, movement, habitat selection, social structure, geographical distribution and reproductive success. Accordingly, in this study was used scat analysis to determine the diet of persian leopards in Sarigol National Park using percent of occurrence index. The results showed that diet of leopards in the Sarigol National Park includes a high diversity of wild boar (Sus scrofa), wild goat (Capra aegagrus), wild sheep (Ovis orientalis), tolai hare (Lepus tolai), common fox (Vulpes vulpes), Indian crested porcupine (Hystrix indica), domesticated animals; dog, horse, bird and plant materials. Wild sheeps have the largest share and rabbits and horses have the lowest share in the diet of this species. Undoubtedly, identifying food habits of leopard and maintaining prey populations can improve management programs and conservation status.Keywords: Food pyramid, Scat, Food habits
Animal physiology
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: ...
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Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: In a quasi-experimental pre/post design, twenty two volunteer healthy men (aged 20-30 years and body fat 10-16%) participated in an exhaustive aerobic exercise (Bruce treadmill test). Serum and saliva samples were collected immediately before and after the exercise test. C-reactive protein was determined by immunophotometry and IL-6 was determined by ELISA method. Data were expressed as mean (±SD) and analyzed by t-test and Spearman's correlation coefficient at α≤0.05. Results: The results show that the serum and saliva CRP and IL-6 were significantly increased (P
Omid Tabiee
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
The objective of this paper was to determine species diversity of wintering waterbirds from a ten-year (2002-2011) monitoring in Meyghan wetland. Data for calculating the estimates of biodiversity come from the midwinter census by using a total count from the department of the Markazi province's environment. ...
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The objective of this paper was to determine species diversity of wintering waterbirds from a ten-year (2002-2011) monitoring in Meyghan wetland. Data for calculating the estimates of biodiversity come from the midwinter census by using a total count from the department of the Markazi province's environment. Data analysis for determine species diversity carried out by using Ecological Methodology and SPSS software. During a 10 periods from 2002 to 2011, a total of 134350 wetland birds composed of 56 species from 6 orders and 16 families of the bird fauna of Iran were observed during the course of this study. The most common species counted was the Common Crane (Grus grus) with a total of 54000 individuals. The results indicated that among families of migratory, wintering waterfowl and waders in Meyghan wetland the large family was Gruidae with 40.19 percent abundance and the lowest was Haematopodidae and Burhinidae each one with 0.01 percent. The highest species richness recording in years of 2010 with 37 species in this year. The results showed that in this period wader's fauna with 62.1 percent and waterfowl fauna with 37.9 percent, respectively has the highest and lowest abundances. According to the results of species diversity and evenness the year of 2010 has the highest biodiversity of migratory, wintering waterfowl and wader's fauna in Meyghan wetland and the year of 2002, 2003 and 2008 has the lowest biodiversity (P < 0.05).
Animal physiology
ramesh monagemi; noushin naghsh
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian ...
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Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian C. medica on Hela cell line. After preparation extract and volatile oils, different concentrations of volatile oils of C. medica (0.25-10 μg / ml) and extract of Citrullus Colocynthis (100-1000 μg / ml) were added to Hela cultured cells and incubated for 72 hours. Cell survival was evaluated using MTT-based cytotoxicity assay. The results showed that methanolic extract of Citrullus Colocynthis had not significant cytotoxic effect on Hela cells but Volatile oils of Citrus medica had significant cytotoxic effect on this cells and it’s most compounds was limonene , linalool , linalool acetate and beta - pinene. The findings showed that volatile oils of C. medica had more cytotoxic effect than the Citrullus Colocynthis extract, limonene and linalool of the volatile oil causes this effects.
Biochemistry
reza Haji Hosseini
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum ...
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Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum total protein was measured by the Bradford method.
Results:
The average amount of protein in the serum of exposure to mustard gas (patients) compared with a control group, showed no significant difference (05/0
Biochemistry
Nayere Bahamin; Behzad Sareghi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
In this study, the interaction of various concentrations of Cadmium Sulfate with Peroxidase (E.C 1.11.1.7) was investigated in different temperatures (25-35 °C). For this purpose thermostability, spectrophotometry, spectroflorimetry and kinetics studies were done to obtain thermodynamic parameters including ...
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In this study, the interaction of various concentrations of Cadmium Sulfate with Peroxidase (E.C 1.11.1.7) was investigated in different temperatures (25-35 °C). For this purpose thermostability, spectrophotometry, spectroflorimetry and kinetics studies were done to obtain thermodynamic parameters including ∆G°, ∆H°m, ∆S°m, Tm and also kinetics parameters including Vmax and Km. The results showed that Cadmium Sulfate caused uncompetitive inhibition at different temperatures and increasing of temperature intensified this inhibition effect. Also Cadmium Sulfate was reduced the stability of peroxidase and reduced its Tm. In the spectrophotometric studies the effect of Cadmium Sulfate on the peroxidase in such a way that the absorption in 275nm increased and temperatures increased it too, also the absorption in 404nm (soret bond) decreased and temperature decreased it too. Totally, Cadmium Sulfate ions in a time- and dose- dependent manner and affected by temperature bind to the peroxidase in the heme environment and decrease the thermostability of it, and exert uncompetitive inhibition.
animal biosystematic
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
To identify the species of sea cucumbers belong to Holothuroidea in the intertidal zones of Chabahar Bay, field studies were conducted during one year from November 2008 to September 2009. The area of study was located at the farthest end of southeast of Iran in province of Sistan and Balouchestan ...
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To identify the species of sea cucumbers belong to Holothuroidea in the intertidal zones of Chabahar Bay, field studies were conducted during one year from November 2008 to September 2009. The area of study was located at the farthest end of southeast of Iran in province of Sistan and Balouchestan between 25°16́́ 61̋ and 25°22́ 47 ̋ North, and between 60°39́ 90 ̋ and 60°24́ 38 ̋ East. 5 sampling stations along the shoreline zone was determined based on geomorphological characteristics, habitat and accessibility. Bimonthly Sampling was carried out in the selected stations in complete tide and all samples of each species were collected. Then samples were transferred to laboratory and identified based on extracted ossicles with valid identification keys .the four species of sea cucumbers were identified: Holothuria leucospilota, Holothuria arenicola, Holothuria insignis, Holothuria sp. Which bolnge to family of Holothuroidae and order of Elasipodida. The Maximum frequency of Holothuroidea Was measured in January (2009) in station 4.
animal biosystematic
Articles in Press, Accepted Manuscript, Available Online from 12 November 2014
Abstract
Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters ...
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Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters including shape, size of shell, dextral and sinistral position of the shell, operculum, aperture, radula, mucus colour, respiratory pore, keel, renal ridge and reproduction system. The most and least distribution among identified snail species are 18 and 8 species belong to 4 and 2 stations respectively from Dez river, which they are 20/82 kilometers away from each other. The most commonly distributed family is Thiaridae with 8 species Melanopsispraemorsa ، Melanopsis costata ،Melanopsis doriae Melanopsis nodosa، Melanopsis subtingitana, Melanoides pyramis, Melanoides tuberculata Thiara scabra. Bulinus truncates and Lymnaea truncatula are identified as the most frequent and has medical importance.Melanopsis subtingitana Annandale,1918 and Gyraulus huwaizahensis species are reported for the first time from Iran.
teimour thanha
Articles in Press, Accepted Manuscript, Available Online from 15 May 2017
Abstract
This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal fistula .first, ...
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This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal fistula .first, The chemical properties include: moisture, ash, fat, lactose and protein were measured. Later, using Tris buffer samples were extracted proteins and the extracted proteins were determined electrophoresis. For nylon bag technique, the amounts of 5 gram of whey powder samples were placed inside the bag. Incubation times were: 0, 2, 4, 8, 16, 24, 48 and 72 hours. Gel electrophoresis showed that the major protein in whey powder consisted of β- lactoglobulin .lactalbumin, bovine serum albumin and immunoglobulin. These proteins are respectively 50, 20, 10 and 10 percent of total whey proteins in powder form nylon bag Results showed that at 0, 2, 4 and 48 hours incubation, WP1 and WP2 and 72 hours incubation, WP2 highest amount of protein degradability (p
Articles in Press, Accepted Manuscript, Available Online from 13 March 2018
Abstract
Abstract
The bilateral relationship between Ventro Medial Nucleus and dopaminergic system may play an important role in the secretion of the gastrointestinal exocrine glands, nutritional behaviors and habits. The aim of this study was to investigate the role of dopaminergic receptors in regulation of ...
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Abstract
The bilateral relationship between Ventro Medial Nucleus and dopaminergic system may play an important role in the secretion of the gastrointestinal exocrine glands, nutritional behaviors and habits. The aim of this study was to investigate the role of dopaminergic receptors in regulation of the exocrine glands and to find out whether the change in saliva secretion is the primary effects of central nervous system or there are subsequences of taking food, lick, swallowing and digestion in the mouth. Ninety rats were stereotaxically implanted under urethane anesthesia. The rats were divided into 9 groups: one control group, one fasted control group, two sham groups given surgery and surgery plus medicine solvents, one SCH23390 group, one bromocriptine group, one mixed group: SCH23390 along with bromocriptine, one pilocarpine and a mixed group: bromocriptine along with pilocarpine. In this study, saliva secretion was gathered from submandibular glands by cannulas and measures then volume of secretion by using SPSS program the groups were compared (Test Anova). The saliva secretion was not significantly different between all groups except SCH23390 group, pilocarpine, and both mixed groups which showed significant differences in the saliva secretion. This study showed that SCH23390 (7.5 μg) could increase saliva secretion. Bromocriptine could not affect saliva secretion. Bromocriptine along with pilocarpine significantly decreased saliva secretion. However, this agonist together with SCH23390 significantly increased saliva secretion. So the dopaminergic system of the Ventro Medial Nucleus has a considerable effect on the secretion of the exocrine glands in the digestive system.
Articles in Press, Accepted Manuscript, Available Online from 29 April 2018
