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Iodine determination in hair with acid digestion and microplate reading procedure

Document Type : Article

Authors

1 . Former M. Sc. Student, Department of Biochemistry, Faculty of Sciences, Payame Noor University, Tehran, Iran

2 Ph. D. Candidate of Biochemistry, Payame Noor University, Tehran, Iran

3 Associate Professor, Cellular & Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Abstract
After iron, iodine deficiency is the most common nutritional deficiency in third world countries. Today, the urinary iodine which indicates the recent iodine status is the only biological indicator for iodine. Therefore, providing of a long-term indicator for assessing of iodine status is essential. In this study, by using the well-known Sandell–Kolthoff reaction the iodine levels were simultaneously measured in hair and urine of 50 samples. The precision, accuracy, and sensitivity of the assay were also evaluated. Acid digestion was sufficiently accurate. Intra and inter-assay coefficients were less than 10 and 12 percent, respectively. Based on the recovery and recycling tests, the recovery percentage of the applied method was 90 to 110 percent which indicates an acceptable accuracy for this study. This method can digest the hair samples in an acidic environment and has enough efficiency to remove interfering and iodine releasing agents in the samples. Moreover, this method has sufficiently the precision, accuracy, and sensitivity required for the measurement of iodine in the hair samples. In addition, using of acidic digestion, reduces the risk of alkaline medium for digestion and the time of long-term ashing methods. It also increases the safety of the used method.

Keywords

References
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Experimental animal Biology
Volume 6, Issue 4
June 2018
Pages 105-112
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