In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Document Type : Article

Authors

1 Assistance of Professor, Faculty of Veterinary Medicine and Urmia Lake Research Institute, Urmia

2 Ph. D. Candidate of Aquaculture, Fishery Department, Faculty of Natural Resources, Urmia University, Iran

Abstract

Abstract
The present study was evaluated to determine effects of levamisole on growth and resistance of rainbow trout against density stress. For this purpose 1000 pieces fish (average weight of 150±6 g) were obtained from a local fish farm of Urmia and were divided in 5 test groups (33 kg/m3 density) and were fed on diet supplemented with levamisole at 0, 100, 250, 500 and 1000 mg per kg of diet for a period of 45 days. Then the fishes of all groups were fed on commercial diet without levamisole and were exposed density stress by 2-3 folds for the following 15 days. Biometry of fishes was done every 15 days and growth factors were measured. Results show that high level of levamisole (1000 mg/kg) had the lowest effect on growth factors of fishes. The maximum survival percent of fishes were observed in 500, 250, and 100 mg/kg Levamisole at the end of research and the level 1000 mg/kg Levamisole caused increase mortality of fishes into control group in density stress significantly (P 

Keywords

Aditya, K.W.; Heinrich, K.; Josie, L.; Gibson, L.; (2008). Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes. Aquaculture; 274: 1-14.
Anderson, D.P.; Siwicki, A.K.; Rumsey, G.L.; (1995). Injection or immersion delivery of selected immunostimulants to trout demonstrate enhancement of nonspecific defense mechanisms and protective immunity. In: Shariff, M., Subasighe, R. P. and Arthur, J. R. Eds. Diseases in Asian Aquaculture Vol. 11. Fish Health Section, Asian Fisheries Society, Manila, Philippines; pp: 413-426.
Asmita, S.; Uday, S.; (2013). Effect of coating of hydrocolloids on chickpea (Cicer arietinum L.) and green gram (Vigna radiate) splits during deep fat frying. International Food Research Journal; 20: 565-573.
Bahram, S.; Vahabzade Roudsari, H.; Mohammad Nazari, R.; Javadian, R.; (2005). Effect of Levamisole on survival of Acipenser persicus. Journal of Marine Science and Technology; Voi, 1&2, No, 4. pp: 1-9.
Booniaratpalin, S.; Booniaratpalin, M.; Supamattaya, K.; Toride, Y.; (1995). Effect of peptidoglucan (PG) on growth, survival, immune responses and tolerance to stress in black tiger shrimp, Penaeus monodon. In: Shariff, M., Subasighe, R. P. and Arthur, J. R. Eds. Diseases in Asian Aquaculture; Vol. 11. Fish Health Section, Asian Fishery Society, Manila, Philippines. pp: 469-477.
Emadi, H.; (2008). Culture of Oncorhynchus and Salmon. Iran, Abzian Publication; pp: 276.
Gholipoor, F.; Allameh, S.; Mohammadi Arani, M.; Nasr Esfahani, M.; (2006). Survey of density effects on growth and food conversion rate of rainbow trout. Journal of Pajoohesh and Sazandegi; 70: 13-27.
Gopalakannan, A.; Arul, V.; (2006). Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture; 255: 179-187.
Hardy, R.W.; (2002). Rainbow Trout, Oncorhynchus mykiss. In: Nutrient requirements and feeding of finfish for aquaculture. Eds., Carl D. Webster, Chorn Lim, London, CABI Publishing; 184-202. 
Hoseini Najdgerami, A.; Manaffar, R.; Meshkini, S.; Salimi, B.; (2007). Survey of initial feeding on rainbow trout fry. Journal of science of Tarbiat Moalem University; 7(1&2): 849.
Jorgensen, J.B.; Lunde, H.; Robertsen, B.; (1993). Peritoneal and head kidney cell response to intraperitoneally injected yeast glucan in Atlantic salmon, Salmo salar L. Journal of Fish Disease; 16: 313-325.
Kitao, T.; Yoshida, T.; (1986). Effect of an immunopotentiator on Aeromonas salmonicida infection in rainbow trout (Salmo gairdneri). Veterinary Immunology Immunopathology; 12: 287-291.
Li, P.; Wang, X.; Delbert, M.; Gatlin, I.; (2006). Evaluation of levamisole as a feed additive for growth and health management of hybrid striped bass (Morone chrysops×Morone saxatilis). Aquaculture; 251: 201-209.
Maqsood, S.; Samoon, M. H.; Singh, P.; (2009). Immunomodulatory and Growth Promoting Effect of Dietary Levamisole in Cyprinus carpio Fingerlings against the challenge of Aeromonas hydrophila. Turkish Journal of Fisheries and Aquatic Sciences; 9: 111-120.
McNeely, R.N.; Neimanis, V.P.; (1979). Water quality sourcebook, a guide to water quality parameter, water quality branch. OTAWA, Canada; 225-253.
Meshkini, S.; Tafy, A.A.; Tokmechi, A,; (2012). Farhangpajooh F. Effect of Chitosan on hematological parameters and stress resistance in rainbow rtout (Oncorhynchus mykiss). Journal of Veterinary Research Forum; 3(1) 49-54. 
Meshkini, S.; (2003). Survey effects of rainbow trout initial feeding by enriched artemia with ascorbic acid on growth, survival and resistance against environmental stresses. Ph. D. thesis of veterinary, Tehran University.; No: 163. 
Nadery Jolodar, M.; Esmaili Sari, E.; Ahmadi, M.; Seifabadi, S.; Abdoli, A.; (2006). Survey rainbow trout farm’s pollution on wwater quality of Haraz River. Journal of Environmental Science; 4(2): 21-36.
Pirhonen, J.; (2003). Effects of anesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture; 220: 507-514.
Shahidi, A.; Vahabzadeh, H.; Zamini, A.; Sadeghpour, A, Amiri, S.; (2012). The effect of levamisole hydrochloride on survival of grass carp (Ctenopharyngodon idella). Proceeding of the International Conference on Ecological, Environmental and Biological Sciences (ICEEBS'2012); Jan 7-8, Dubai. DOI: 10.13140/2.1.4786.6880.
Siwicki, A.K.; (1988). Korwin-Kossakowski M. The influence of levamisole on the growth of carp (Cyprinus carpio L.) larvae. Apply Ichthyology; 4: 178-181.
Sukhoverkhov, F.M.; (2006). The effect of cobalt, vitamin, tissue preparations and antibiotics on carp production; http://www.FAO.com
Symoens, I.; Rodenthall, M.; (1977). Levamisole in the modulation of the immune responses. The current experimental and clinical state. Journal of Reticuloendothelial Soc.; 21: 175-221.
Yazdanbakhsh, A.; Sheykhmohammadi, H.; Bonyadinejad, R.; Ghanbari, G.H.; (2009). Survey Nitrite and Nitrate concentration in potable water of Tehran (The zone under control of Shahid Beheshti Medical Science University). Proceeding of 12th national congress of environment hygiene; September, Shahid Beheshti Medical Science University, Iran.