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Biosystematics of species belonging to the genera LucioBarbus ‎and Capoeta based on scale morphology

Document Type : Article

Authors

1 Department of Animal Biology, ‎Shahrekord University, Shahrekord, ‎Iran‎

2 Department of Fisheries, Faculty of ‎Natural Resources and Earth ‎Sciences, Shahrekord University, ‎Shahrekord, Iran

10.30473/eab.2026.74723.1994

Abstract

There are more than 200 species of freshwater fish in Iran. In addition to the ability to identify fish, scales are the only available samples in cases such as checking food rations or checking historical biodiversity in aquatic environments. In this regard, in this research, with the aim of preparing the basic data of the scales in the genera Capoeta and LucioBarbus, samples including ten species were prepared from the Tigris, Karun, Ker, Namak and Caspian basins.In order to check the prepared samples, 10 to 15 scales were prepared from the areas of the cap, under the dorsal fin and on the caudal stem, and after treatment in 5% potassium solution and fixation on the slide using a microscope and a loupe equipped with a camera. became The landmarking operation and investigation were carried out using the detection function analysis and principal component analysis methods, respectively, using TpsDig and PAST software.Based on the obtained results, it was found that the scales have gender-specific appearance differences, the caudal stem scales separated the species more accurately. Examining the geometric shape using landmarks was the best for separations at the genus level. Among the examined species, LucioBarbus braczcephalus, LucioBarbus capito and Capoetasaadi were distinguished from other species with the highest accuracy.

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References
Abasi Dehkord, I., Hashemzadeh Segherloo, I., Poria, M., & Khajeh, P. (2018). Analysis of phylogenetic status of LucioBarbus esocinus, LucioBarbus xanthopterus, Tor grypus, and Mesopotamichties sharpeyi. Journal of Fisheries, 71(2), 159-166.
Barman, B. (2024). Beyond Copyright Walls: An Introduction to Creative Commons, Copyleft, and Open Educational Resources. In V. K. Ahuja & K. Baishya (Eds.), Research and Publication Ethics (pp. 229–250). Knowledge Resource Center, National Law University and Judicial Academy.
Braeger, Z., Staszny, A., Mertzen, M., Moritz, T., & Horvath, G. (2017). Fish scale identification: from individual to species-specific shape variability. Acta Ichthyologica et Piscatoria, 47,331-338.
Caliendo, A. M., Gilbert, D. N., Ginocchio, C. C., Hanson, K. E., May, L., Quinn, T. C., & Bonomo, R. A. (2013). Better tests, better care: improved diagnostics for infectious diseases. Clinical Infectious Diseases, 57(suppl_3), S139-S170.
Cantonati, M., Poikane, S., Pringle, C. M., Stevens, L. E., Turak, E., & Heino, J. (2020). Characteristics, main impacts, and stewardship of natural and artificial freshwater environments: consequences for biodiversity conservation. Water, 12(1), 260.
Coad, B. W., & Vilenkin, B. Y. (2004). Co-occurrence and zoogeography of the freshwater fishes of Iran. Zoology in the Middle East, 31(1), 53-62. 
Esmaeili, H. R., & Gholami, Z. (2011). Scanning Electron Microscopy of the scale morphology in Cyprinid fish, Rutilusfrisii kutum Kamenskii, 1901 (Actinopterygii: Cyprinidae).
Fischer, J. (2014). Fish identification tools for biodiversity and fisheries assessments: review and guidance for decision-makers. FAO Fisheries and Aquaculture Technical Paper(585), I.
Hashemzadeh Segherloo I., Abdoli A., & Purahmad R. (2015). Phylogenetic and geographic relationships of some species of the genus Capoeta in the Tigris, Persian Gulf, Kerman-Naeen, Namak, Tezdjen, and Caspian basins. Journal of Genetics, 10(2), 229-238.
Hooper, A. K., & Bonduriansky, R. (2022). Effects of genetic vs. environmental quality on condition‐dependent morphological and life history traits in a neriid fly. Journal of Evolutionary Biology, 35(6), 803-816.
Khaefi R., Esmailie, H.R., Ansari M., & Ebrahimi, M. (2018). Hashemzadeh Segherloo I., Abdoli A., Purahmad R, (2015). Phylogenetic and geographic relationships of some species of the genus Capoeta in the Tigris, Persian Gulf, Kerman-Naeen, Namak, Tezdjen, and Caspian basins, Journal of Genetics, 10(2), 229-238. 
Levin, B.A., Freyhof, J., Lajbner, Z., Perea, S., Abdoli, A., Gaffaroğlu, M., Özuluğ, M., Rubenyan, H.R., Salnikov, V.B., & Doadrio, I., 2012. Phylogenetic relationships of the algae scraping cyprinid genus Capoeta (Teleostei: Cyprinidae). Molecular phylogenetics and evolution, 62(1),542-549.
Manandhar, P., Khalid, M., & Labh, S. (2024). Spatiotemporal variability in water quality, ichthyofaunal diversity, and its conservation in the rivers of Nepal: A mini review. Iranian Journal of Fisheries Sciences, 23(4), 633-648.
Marenkov, O. (2018). Laboratory manual on general and special ichthyology. World News of Natural Sciences, 18(1), 1-51.
Papadakis, V. M., Papadakis, I. E., Lamprianidou, F., Glaropoulos, A., & Kentouri, M. (2012). A computer-vision system and methodology for the analysis of fish behavior. Aquacultural engineering, 46, 53-59.
Pinna, M., Zangaro, F., Saccomanno, B., Scalone, C., Bozzeda, F., Fanini, L., & Specchia, V. (2023). An overview of ecological indicators of fish to evaluate the anthropogenic pressures in aquatic ecosystems: from traditional to innovative DNA-based approaches. Water, 15(5), 949.
Poulet, N., Reyjol, Y. , Collier, H., & Lek, S. (2005). Does fish scale morphology allow the identification of populations at a local scale? A case study for rostrum dace Leuciscus leuciscus burdigalensis in River Viaur (SW France), Aquatic Sciences, 67, 122-127 
Reid, G. M., Contreras MacBeath, T., & Csatádi, K. (2013). Global challenges in freshwater‐fish conservation related to public aquariums and the aquarium industry. International Zoo Yearbook, 47(1), 6-45.
Rohlf, F.J. (2010). tpsDig. Stony Brook, NY: Department of Ecology and Evolution, State University of New York.
Roswell, M., Dushoff, J., & Winfree, R. (2021). A conceptual guide to measuring species diversity. Oikos, 130(3), 321-338.
Tabatabei, S.N., Eagderi, S., Hashemzadeh Segherloo, I., & Abdoli, A. (2013). Geometric and morphometric analysis of fish scales to identity genera, species and populations case study: the Cyprinid family. Taxonomy and Biosystematics, 5(17), 1-8.
Westlund, L. (2007). Disaster response and risk management in the fisheries sector (Vol. 479): Food & Agriculture Org.
Yousefi, M., Jouladeh-Roudbar, A., & Kafash, A. (2020). Using endemic freshwater fishes as proxies of their ecosystems to identify high priority rivers for conservation under climate change. Ecological Indicators, 112, 106137.
Experimental animal Biology
Volume 14, Issue 2 - Serial Number 54
September 2025
Pages 31-43
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