Document Type : Article
Authors
Abstract
Abstract
In order to identify biodiversity of plant parasitic nematodes in alfalfa and no comprehensive research about the plant parasitic nematodes associated with alfalfa,during 2002-2003, 33 soil samples were collected from alfalfa fields of several locations in alfalfa fields in Ahar region, partly biodiversity plant parasitic nematodes in this crop were identified.Nematodes extracted by combined sieving and centrifugal-floatation Jenkinse method and transferred to glycerine by De Grisse,s method. After preparation pemanent microscopically slides examined under the light microscope. Measurements and drawing of nematodes were carried out using by drawing tube equipped the microscope. Different species were identified on the basis of morphological characters as well as morphometric parameters and available literature. In this survey 20 species of 11 genera belonging sub order tylenchina included: Boleodorus thylactus, Coslenchus aquaticus, Criconemella antipolitana, Ditylenchus dipsaci, Ditylenchus medicaginis, Geocenamus brevidens, Geocenamus nanus, Geocenamus quadrifer, Helicotylenchus digonicus, Helicotylenchus pseudorobustus, Helicotylenchus vulgaris, Paratylenchus italiensis, Paratylenchus tateae, Pratylenchus scribneri, Pratylenchus neglectus, Pratylenchus thornei, Psilenchus iranicus, Tylenchoryhnchus parvus, Tylenchoryhnchus striatus, Zygotylenchus guevarai. Among the recognized genera and species, Geocenamus nanus, Tylenchoryhnchus striatus and Tylenchoryhnchus parvus are reported for the first time from lucerne fields of Iran.
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