Fariba Yaghmori; Reza Hajihosseini; Seyed Mehrdad Kassaee; Bahram Sefizarei
Abstract
Sepsis is a systemic body reaction to invasive microorganisms such as bacteria and fungi It has also been a systemic response to severe infections it is one of the top ten main causes of death among all patients admitted to the hospital. Multiple potential drug therapies have been investigated, ...
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Sepsis is a systemic body reaction to invasive microorganisms such as bacteria and fungi It has also been a systemic response to severe infections it is one of the top ten main causes of death among all patients admitted to the hospital. Multiple potential drug therapies have been investigated, but as yet there is no known effective pharmacological treatment for sepsis. Therefore, the effect of aspirin as a non-steroidal anti-inflammatory drug (NSAID) in the treatment and reduction of sepsis effects on parameters involved in oxidative damage to liver tissue has been investigated. For this purpose, the experimental inflammatory model was used in this study, so that the mice were divided into four groups (4 people). The first group - control group, the second group-LAP group (laparotomy), the third group-CLP group; Group 4 - Aspirin treatment group with a dose of 2 mg / kg body weight orally once a day for 48 hours after induction of CLP in rats and blood samples were collected from their hearts. In the next step, the animals are killed and the liver tissue is separated for histological and biochemical studies. Separated liver tissue, to test for COX2 gene expression; The Real-Time pCR technique was used. Statistical analysis was performed using SPSS software and Anova test. p < 0.05 is considered statistically significant. Our results showed that the treatment of animals with aspirin is effective in regulating antioxidant and inflammatory parameters. Also, the findings indicate that in liver tissue, aspirin has the greatest effect on reducing gene expression. Pathological studies have also shown that sepsis causes damage to liver tissue that can be reduced by these methods. Finally, sepsis causes oxidative damage to liver tissue and the use of aspirin is effective in preventing and improving these injuries.
Fariba Yaghmori; Reza Hajihosseini; Seyed Mehrdad Kassaee; Bahram Seifizarei
Abstract
Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly ...
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Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly divided into six experimental groups. Rats were treated with indomethacin, celecoxib and aspirin orally with dose of 2 mg/kg b.w for 48h after CLP (Cecal ligation and puncture) injury. Then, blood samples were collected to determine serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and Bilirubin. The extracted livers were used for biochemical assays [Malondialdehyde (MDA), glutathione (GSH) and myeloperoxidase (MPO) activities] and real time PCR studies. The result revealed that treatments significantly improved antioxidant and liver enzymes by reducing MDA, MPO, AST and ALT level and increasing level of GSH. Moreover, cyclooxygenase-2(COX-2) expression in the liver tissue was decreased in the treatment group compared to the CLP group. Thus, our result suggests CLP induced oxidative hepatic damage and NSAIDs have the potential for the treatment of liver damage consecutive to chemical intoxication.
