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Anti-inflammatory and ‎antioxidant activity of aspirin in ‎septic animal model and Take a ‎look at liver enzymes

Document Type : Article

Authors

1 Ph. D. in Biochemistry, Department of Biology, Payame Noor ‎University, Tehran, Iran ‎

2 Professor, Department of Biology, Faculty of Science, Payame ‎Noor University, Tehran, Iran

3 Assistant Professor of Biochemistry, Department of Biology, ‎Islamic Azad University, Hamedan Branch, Hamedan, Iran

4 Researcher, Gastroenterologist and Hepatologist Middle East ‎Liver Disease Center, Hamedan, Iran

Abstract

Sepsis is a systemic body reaction to invasive microorganisms such as bacteria and fungi It has also been a systemic response to severe infections it is one of the top ten main causes of death among all patients admitted to the hospital.  Multiple potential drug therapies have been investigated, but as yet there is no known effective pharmacological treatment for sepsis. Therefore, the effect of aspirin as a non-steroidal anti-inflammatory drug (NSAID) in the treatment and reduction of sepsis effects on parameters involved in oxidative damage to liver tissue has been investigated. For this purpose, the experimental inflammatory model was used in this study, so that the mice were divided into four groups (4 people). The first group - control group, the second group-LAP group (laparotomy), the third group-CLP group; Group 4 - Aspirin treatment group with a dose of 2 mg / kg body weight orally once a day for 48 hours after induction of CLP in rats and blood samples were collected from their hearts. In the next step, the animals are killed and the liver tissue is separated for histological and biochemical studies. Separated liver tissue, to test for COX2 gene expression; The Real-Time pCR technique was used. Statistical analysis was performed using SPSS software and Anova test. p < 0.05 is considered statistically significant. Our results showed that the treatment of animals with aspirin is effective in regulating antioxidant and inflammatory parameters. Also, the findings indicate that in liver tissue, aspirin has the greatest effect on reducing gene expression. Pathological studies have also shown that sepsis causes damage to liver tissue that can be reduced by these methods. Finally, sepsis causes oxidative damage to liver tissue and the use of aspirin is effective in preventing and improving these injuries.

Keywords

References
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Experimental animal Biology
Volume 9, Issue 3 - Serial Number 35
January 2021
Pages 11-17
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