Fisheries
Azam Ghorbannia Delavar; Saeed Alinejad Moallem
Abstract
Diazenone is one of the most important organospheric pesticides that is widely used in agriculture and homes to control insects in the soil, plants and other crops. Diazenone is used to control plant pests in many agricultural fields that are located near fresh water sources, It is common.Therefore, ...
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Diazenone is one of the most important organospheric pesticides that is widely used in agriculture and homes to control insects in the soil, plants and other crops. Diazenone is used to control plant pests in many agricultural fields that are located near fresh water sources, It is common.Therefore, in this research, the effect of agricultural poison diazenon was investigated by calculating LC50 on blood parameters and lactobacilli in the intestine of Hypophthalmichthys nobilis. In this experiment, fish were exposed to different concentrations of diazenon poison for 15 days. The amount of blood parameters of red and white blood cells and the presence of lactobacillus species were investigated in 6 treatments with 3 repetitions over a period of 15 days. Then, blood sampling and sampling from the intestines of Hypophthalmichthys nobilis were done. The results showed that the hematological indices of Red carp exposed to different doses of diazenon poison showed a significant decrease in RBC and WBC with increasing poison concentration (p<0.05). Also, the results showed that in all different doses of diazenon poison, the lack of growth of Lactobacillus bacteria compared to the control group on MRS agar culture medium had significant differences (p<0.05). Finally, it can be mentioned that the use of intestinal and blood Lactobacillus samples to investigate the microbial and hematological indicators of Sargandeh carp can be used as biomarkers for measuring and tracking the effects of diazenon poison in aquatic communities.
Morteza Kamali; Saeid Alinejad Moaelem; Nastaran Pour-Ahmadi
Abstract
Abstract Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and ...
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Abstract Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and the aquatic and human populations. In present study; effects of toxicity of DEHP on protein band model and amino acid profile of mucus common carp (Cyprinus carpio) was studied. 108 common carp (17.60±2.22gr) is gathered from warm water fish center and they were allocated into 9 tanks (12 Fish per tank). Fish adapted to new condition for a week. During this time; they were feed twice a day with same diets. Fishes were exposed at 3 concentration treatments containing 0; 0.1 and 1 mg/L DEHP under laboratory condition (25±0.5°C; pH: 7.4-8) for a period of 96 hours. We assessed skin mucus protein profiles by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and we were studied amino acid profile by HPLC. The results of the experiments to compare the protein pattern of carp’s mucus (exposed to DEHP with two different concentrations) with the control sample (without exposure to DEHP) showed that there is a significant difference in the bands density between the two protein patterns. These results indicated that concentrations of DEHP beneficially affects Amino Acid profiles of skin mucus in carp and they were categorized in 4 branches. There were Significant differences between exposed and control groups. Therefore; fish skin mucus can be a biomarker for showing contaminants and toxins. And the high consumption of plastic materials can be a warning to the health of aquatic animals and humans.
