Biochemistry
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: ...
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Background: Based on the limited and inconsistent results about the effects of exercise on inflammatory markers in serum and saliva, this study was conducted to comparing of serum and saliva C-reactive protein (CRP) and interleukin-6 responses in healthy men following one bout exhaustive aerobic exercise.Methods: In a quasi-experimental pre/post design, twenty two volunteer healthy men (aged 20-30 years and body fat 10-16%) participated in an exhaustive aerobic exercise (Bruce treadmill test). Serum and saliva samples were collected immediately before and after the exercise test. C-reactive protein was determined by immunophotometry and IL-6 was determined by ELISA method. Data were expressed as mean (±SD) and analyzed by t-test and Spearman's correlation coefficient at α≤0.05. Results: The results show that the serum and saliva CRP and IL-6 were significantly increased (P
Davoud Fazli; Aliakbar Malekirad
Abstract
Reactive oxygen species (ROS) are cytotoxic agents that lead to significant oxidative damage. Bisphenol A (BPA) is a contaminant toxic, oxidative and estrogenic effects. Purslane is a promising natural product, which could be useful for the prevention of chronic diseases caused by oxidative stress. In ...
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Reactive oxygen species (ROS) are cytotoxic agents that lead to significant oxidative damage. Bisphenol A (BPA) is a contaminant toxic, oxidative and estrogenic effects. Purslane is a promising natural product, which could be useful for the prevention of chronic diseases caused by oxidative stress. In this study 49 adult male rats (250 10g) were divided randomly in 7 groups (n=7): Control, Sham (500 ug corn oil\Kg BW), Toxin group (500ug BPA\kg BW), Positive control (100mg purslane hydroalcoholic extract\kg BW) and 3 purslane treated groups (500ug BPA + 50,100&150 mg purslane\kg BW). All injections are done by i.p rout in 14 consequently days. At the end of administration time (14 day) all animals were anesthetized with Chloral hydrate and sacrificed, then liver samples isolated to preparing tissue homogenates for measure antioxidant enzymes including SOD, CAT, TAC, GPX and MDA. Data are analyzed in SPSS statistical software by one way ANOVA with Tukey posttest. Analyzed data showed that MDA, TAC, CAT, GSH and GPX measures are modified in purslane treated groups compared to the bisphenol A group which indicates that the portulaca oleracea extract can improve the hepatotoxicity of bisphenol A induced.
Fatemeh Nabiyouni; Gholamhasan Vaezi; Ali Akbar Malekirad; Mohammad Abdollahi
Volume 5, Issue 1 , September 2016, , Pages 31-38
Abstract
Diabetes is one of the most common metabolic diseases and in the long term affects many parts of body, including liver and kidneys. This study was to investigate alcoholic extract effect of Alhagi camelorum on functional tests of liver and kidney in diabetic rats induced by streptozotocin. In this study, ...
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Diabetes is one of the most common metabolic diseases and in the long term affects many parts of body, including liver and kidneys. This study was to investigate alcoholic extract effect of Alhagi camelorum on functional tests of liver and kidney in diabetic rats induced by streptozotocin. In this study, 28 Wistar male rats were divided into the four groups (n = 7): non-diabetic control group received normal diet, diabetic group was induced by streptozotocin and received 1ml of normal saline daily as extract solvent , two diabetic experimental groups received alcoholic extract of Alhagi Camelorum at dose of 200 mg/kg and 300 mg/kg by gavage, respectively. To induce diabetes, streptozotocin was injected to rats intraperitoneally at dose of 50 mg/kg. Blood samples were collected at day 21 from all groups and the related blood factors were measured and analyzed by SPSS (ANOVA). The findings show that there was a significant increase in creatinine, urea levels and liver enzymes in diabetic control group when compared to the non-diabetic control group. While the same amount factors show decrease in the groups receiving alcoholic extract of Alhagi Camerolum. The results show that alcoholic extract of aerial parts of Alhagi Camerolum causes to the decreased creatinine, urea levels and liver enzymes.
A. Jafari; L. Satarzadeh; F. Askarian; A. Akbar malekirad
Volume 2, Issue 2 , September 2014, , Pages 35-43
Abstract
This study was conducted to compare of inflammatory markers response in serum and saliva of healthy men following an exhaustive exercise test. Twenty two volunteer healthy men (aged 20-30 years and body fat 10-16%) in a quasi-experimental, single group design participated in Bruce exercise test. Serum ...
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This study was conducted to compare of inflammatory markers response in serum and saliva of healthy men following an exhaustive exercise test. Twenty two volunteer healthy men (aged 20-30 years and body fat 10-16%) in a quasi-experimental, single group design participated in Bruce exercise test. Serum and saliva samples were collected immediately before and after the exercise test. C-reactive protein (CRP) and interleukin-6 (IL-6) were determined by Immunoturbidimetric and ELISA methods, respectively. Data were expressed as mean (±SD) and analyzed by paired t-test and Spearman at α≤0.05. The serum and saliva CRP and IL-6 were significantly increased (P<0.05) after the exercise test. Moreover, the correlation between serum and salivary samples for CRP (rPre=0.82 and rPost= 0.88) and IL-6 (rPre=0.42 and rPost= 0.54) was significantly positive (P<0.05). According to the present results, it can be concluded that saliva CRP and most likely IL-6 samples a suitable and noninvasive alternative for use in order to evaluate exercise-induced systemic inflammation.
