Sh Ghazanfari; Z Mohammadi
Volume 4, Issue 3 , January 2016, , Pages 21-30
Abstract
Abstract Dietary essential oils may be used as alternatives to antibiotics and its might improve poultry performance. The aim of this study was to investigate the effects of coriander essential oil on growth performance, carcass characteristics and immune system in broiler chickens. Two hundred day-old ...
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Abstract Dietary essential oils may be used as alternatives to antibiotics and its might improve poultry performance. The aim of this study was to investigate the effects of coriander essential oil on growth performance, carcass characteristics and immune system in broiler chickens. Two hundred day-old broiler chickens (Ross 308) were allocated to 5 treatments, 4 replications with a completely randomized design during 42 days of age. Experimental treatments consisted of control treatment (basal diet) or basal diets containing different levels of coriander essential oil (100, 200 and 300 mg/kg) and 600 mg/kg flavophospholipol antibiotic. Weight gain, feed intake and feed conversion ratio were measured weekly. Results in total period showed that highest feed intake was observed in antibiotic compared with 100 and 200 mg/kg coriander essential oil treatments (P
SH. GHAZANFARI; H. SEPEHRI MOGHADDAM; K. NOBARI
Volume 3, Issue 2 , March 2015, , Pages 1-9
Abstract
The aim of present research was to study influence of different levels of threonine amino acid (equal to requirements of NRC (1994), average of NRC (1994) requirements and ROSS (2007), requirements ROSS (2007) and more than requirements of ROSS (2007)) and age after hatching on interleukin-8 (IL8) gene ...
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The aim of present research was to study influence of different levels of threonine amino acid (equal to requirements of NRC (1994), average of NRC (1994) requirements and ROSS (2007), requirements ROSS (2007) and more than requirements of ROSS (2007)) and age after hatching on interleukin-8 (IL8) gene expression in intestinal tissue in broiler chickens. Threonine requirements in accordance with the recommendations ROSS (2007) are 14% higher than NRC (1994). In the first experiment, effect of threonine on IL8 gene expression in the jejunum in Ross male broilers and also in the second experiment, effect of age on IL8 gene expression was studied after hatching. In the first experiment, different levels of threonine were: 1) 0.8%, 2) 0.87, 3) 0.94 % and 4) 1.01%. At 14 days, one bird from each replicate was killed and the jejunum tissue samples were obtained. In the second experiment, from first experiment 1 treatment that level of threonine amino acid diet was 0.8%, at 1, 3, 6 and 9 days, a bird killed and jejunum tissue samples were obtained in accordance with first experiment. Trizol kit was used for RNA extraction. Then remove genomic remnants and cDNA was synthesized. Realtime PCR reactions using primers and SYBR Green kit was performed. Experiments results showed that IL8 mRNA expression in jejunum tissue increased during the first 14 days of live chickens with increase threonine amino acid level until 0.94 percentage diet (P<0.0001) and also with age increase (P<0.01).