Frangis Ghassemi
Abstract
Abstract
Fauna identification of animal taxon is one of the basic parameters for biodiversity protection. Bats are useful animal in the ecosystem that is threatened seriously duo to several reasons, as the destruction of their habitat. So in this study, the diversity of bats in 36 caves in the 4 climates ...
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Abstract
Fauna identification of animal taxon is one of the basic parameters for biodiversity protection. Bats are useful animal in the ecosystem that is threatened seriously duo to several reasons, as the destruction of their habitat. So in this study, the diversity of bats in 36 caves in the 4 climates of Fars province was investigated during 10 months. For this purpose, the bats were photographed in the cave during flying or capturing by mist net. The species were identified according special taxonomic keys and confirmed by local and foreign authorities some of bats were abandoned in the site and others were transferred to lab for exactly identification. The obtained results showed 15 species belonging to 8 genera of 7 families as the big colonies of different species lived in the mist and large caves with height and depth. Family Rhinolophidae with 5 species was the most diverse family, and Rhinopoma had the most density and diversity in the harm and temperature regions, respectively. According to obtained results, Fars province is the suitable roost for bats, so the conservation of these habitats must be done for protection of their population by Department of Environment, and their cooperation with caving teams is necessary.
Development
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; ...
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Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; the released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometric analysis were done for CD105, CD90, CD146, CD31 and CD34 at third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated at the third passage after 21 days of induction.The flowcytometry analysis showed that EnSCs were positive for CD90, CD105, CD146 and were negative for CD31, CD34. Moreover we show that the hEnSCs can differentiate in osteocyte and adipocyte, as confirmed by alizarin Red and oil-Red O staining. These differentiated cells into neuron and endothelial express related markers including Nestin, MAP2 and CD31. Results show that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.
Morteza Naderi; Saeid Mohammadi; Neda Behdarvand
Volume 6, Issue 4 , June 2018, , Pages 113-121
Abstract
Abstract Mitochondrial genes important tool in various studies in the fields of animal evolution, is phylogeography and phylogenetic. Based on the analysis carried out by sequencing mitochondrial Cytochrome b gene, five samples of Iranian jerboa and two Hotson's Jerboa from the Mirabad plains and Chupanan ...
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Abstract Mitochondrial genes important tool in various studies in the fields of animal evolution, is phylogeography and phylogenetic. Based on the analysis carried out by sequencing mitochondrial Cytochrome b gene, five samples of Iranian jerboa and two Hotson's Jerboa from the Mirabad plains and Chupanan in Naein, Isfahan Province, respectively, were sampled, it was found that the divergence between the two groups, but this degree of intraspecific divergence between the two species is not enough to lead to the divergence. However, concludes that different habitat conditions, different diet and feeding behavior induced evolutionary divergence and this process in the future, especially in the absence of gene flow between populations may lead to speciation. Extensive studies and more accurate than relying on other molecular markers such as microsatellites and nDNA is suggested.
Ali Asghar Zarei; Maziar Mahmoudi
Abstract
Abstract One step in wildlife management is knowing as target population density and that is required cognition of species dispersion pattern in landscape level. In this research, the dispersion and density of brown bear (Ursus arctos syriacus) with use of the Standardized Morisita Index (SMI) and Adaptive ...
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Abstract One step in wildlife management is knowing as target population density and that is required cognition of species dispersion pattern in landscape level. In this research, the dispersion and density of brown bear (Ursus arctos syriacus) with use of the Standardized Morisita Index (SMI) and Adaptive cluster sampling (ACS) were determined in Arsanjan County range in Fars province. Field work and cencus of presence signs does with use random sample in 3×3 km cells. Total of sampeling grid in study area include 300 cells. Results SMI showed that dispersion pattern brown bear is clumped and individuals that most aggregationed in koh khom non hunting area. The Results of ACS related to active winter dens showed the brown bear density was 0.031 bear in each cell and 0.0034 bear in kilometer square. Therefor recommendation winter dens are appropriate index in evaluation brown bear population and use in density estimate this species in other areas.
Sajjad Rajabi; , Fatemeh Shahbazi; Ali Noori; Raziye Karshenas
Abstract
Abstract Since copper nanoparticles are one of the first nanoparticles planned in the industry, recent research has shown the pathological toxicity of these nanoparticles in different tissues and organs. Therefore, the purpose of this study was to investigate the effect of copper oxide nanoparticles ...
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Abstract Since copper nanoparticles are one of the first nanoparticles planned in the industry, recent research has shown the pathological toxicity of these nanoparticles in different tissues and organs. Therefore, the purpose of this study was to investigate the effect of copper oxide nanoparticles on gonadal tissues in male rats. In this study, 40 Wistar rats were divided in four groups control group and 10, 20, 30 (mg/ kg) of copper oxide nanoparticles, respectively 5 Times, received for one day in a row for 10 days. In order to histopathologic studies, the testicular tissue samples were stained with hematoxylin-eosin. The data were analyzed in the spss software, one –way anova and duncan's test. The results showed that the amount of spermatogonial cells, primary spermatocyte, spermatid cells were decreased in dose-dependent manner. In the testicle, disorders such as abnormality and severe deformity with different morphology in the spermicidal tubes and destruction of sertoli cells were observed. The results of the studies showed that copper oxide nanoparticles with oxidative stress and cellular degradation disrupted the structure and process of gonadal spermatogenesis which due to the absence of mortality in mice may eventually overcome disturbances in normal conditions.
Omid Tabiee; Abdulah Yousefi
Volume 6, Issue 2 , December 2017, , Pages 119-126
Abstract
The objective of this study was to determine the level of Khuzestan people’s knowledge and willingness to pay towards the protection of the Persian Follow Deer (Dama dama mesopotamica (Brooke, 1875)) as endangered species, to recommend solutions that can improve management and protection of this ...
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The objective of this study was to determine the level of Khuzestan people’s knowledge and willingness to pay towards the protection of the Persian Follow Deer (Dama dama mesopotamica (Brooke, 1875)) as endangered species, to recommend solutions that can improve management and protection of this species. In this descriptive and analytical study, 600 people from Khuzestan province were chosen via the clustered random sampling. Data was collected by a questionnaire with a reliability of 0.78, consisting of questions about the people’s demographics as well as their knowledge and practice regarding conservation of Persian Follow Deer was completed for all the study population through interviews. Subsequently, descriptive statistics, x2, and Mann-Whitney test and kruscal-wallis test were employed for analysis using SPSS Software (version 21). The findings of this study showed that, 67 percent of the people had poor awareness about endangered species of Persian Follow Deer and 82.8% of people were not interested in supporting the preservation of this species. On the other hand, there was a significant correlation between knowledge and sex, awareness and levels of education and knowledge and age (P< 0.05). Consequently, with low- level knowledge of the people’s under the study about Persian Follow Deer protection and conservation, it seems advisable that regular and systematic training programs be devised in relation to population decrease prevention strategies such as practical classes on the protection of the population of this species.
Saeed Esmaeili-Mahani2; Mehri Bahaaddini; Saeed Khatamsaz; Mehdi Abbasnejad; Maryam Raoof
Volume 7, Issue 1 , September 2018, , Pages 119-132
Abstract
Abstract Orexin A and orexin B are hypothalamic neuropeptides that have critical roles in the regulation of physiological responses such as pain. C-fos is neural activity markers in painful situations. This study was designed to evaluate the role of trigeminal nucleus caudalis (TNC) orexin 1 receptor ...
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Abstract Orexin A and orexin B are hypothalamic neuropeptides that have critical roles in the regulation of physiological responses such as pain. C-fos is neural activity markers in painful situations. This study was designed to evaluate the role of trigeminal nucleus caudalis (TNC) orexin 1 receptor on capsaicin-induced orofacial pain and c-fos expression in TNC. In this experimental study, 70 male Wistar rats (200-250 g) were randomly allocated into 10 groups: control, pain, sham surgery, sham capsaicin, surgery and pain, sham orexin A and pain, orexin A (50 pM/rat) and pain, sham SB-334867 and pain, SB-334867 (40 nM/rat) as antagonist and pain, orexin A plus SB-334867 and pain. Following cannula implantation and recovery period, the drugs were administration 20 min before capsaicin injection. C-fos expression in TNC was evaluated using immunohistochemistry. The capsaicin-injected rats showed a significant pain behavior and expression c-fos (p
Amer abdollah nezhad banaderi; Soheila Ebrahimi
Abstract
Abstract Pearl oyster Pinctada radiata is belongs to the family Pteriidae and is one of the pearl oyster Persian GOlf. This study was amid to determine the optimum amount of salt and its effects on the filtration Pearl maker incidents using phytoplankton Isochrysis aff galbana in five treatments ...
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Abstract Pearl oyster Pinctada radiata is belongs to the family Pteriidae and is one of the pearl oyster Persian GOlf. This study was amid to determine the optimum amount of salt and its effects on the filtration Pearl maker incidents using phytoplankton Isochrysis aff galbana in five treatments salinity (20, 25, 30, 35 and 40 parts per thousand), and 3 were repeated. Shells with an average length (dorsal-ventral) 49.67± 6.98 mm it was gathered from the Hendorabi island. The initial density of phytoplankton to town with 100,000 cells / ml were considered and at the time of one-hour and two-hour congestion they were counted again. The most of filtration rate was in 35 parts per thousand salinity that it is the first time 2459.77± 89.46 and 14.66% increase in the second hour with 2820.39±57 /00ml / hour / shellfish with other treatments were significantly different (P˂0.05). The filtration rate was about 20 ppt salinity levels in the first hour 140.51±37.02 and in the second hour with 32.12% decrease, 40.55±22.11 ml/ h/ shellfish with Other treatments that were significantly different (P˂0.05). The results showed that best salt for biological activity such as eating, breathing and growing incidents of pearl was in 35 ppt salinity also increases the filtration at second hour in 30, 35 and 40 parts per thousand reflects its ability to adapt in salinity.
Bibi Fatemeh Fani Yazdi; Esmail Fattahi; Naser Mahdavi Shahri; Mohammad Mahdi Ghahramani Seno
Abstract
The interaction between ECM and cells plays an important role in the direction of cellular behavior. So far, different scaffolds have been developed to examine the behavior of cells in three-dimensional conditions. The aim of this study was to investigate the interaction between the mesenteric decellularized ...
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The interaction between ECM and cells plays an important role in the direction of cellular behavior. So far, different scaffolds have been developed to examine the behavior of cells in three-dimensional conditions. The aim of this study was to investigate the interaction between the mesenteric decellularized scaffold of the bovin and the blastema tissue originated from the pinna of New Zealandwhite rabbit. Mesenteric bovine tissue (5 mm×5 mm) was decellularized using physical methods (slow freezing and snap freeze–thaw) and chemical agents (sodium dodecyl sulfate (SDS) and Triton Х-100) followed by washing and sterilization procedures.These parts were assembled as scaffolds inside the blastema rings from rabbit’s pinna. Sampling was carried out on days 7, 10, 15 and 21. Interactions between the scaffolds and the blastema tissue cells were studied by histological and fluorescence microscopy and electron microscopy.The study of the behavior of Blastema cells in different days of culture in addition to the migration and maintenance of Blastema cells on mesenteric decellularized scaffold showed that these scaffolds were able to restore the structure of blood capillaries, fibroblast and fat cells.Based on histological findings, the results indicate that the Blastema tissue has dynamic cells that can migrate into the scaffold.Furthermore the characteristics of the mesenteric decellularized ECM can support adhesion, migration and differentiation of blastema cells in vitro.
Jaber Aazami; Saeed Seyed Ghasemi; Rasool Zamani-Ahmadmahmoudi
Volume 6, Issue 1 , May 2017, , Pages 133-147
Abstract
Birds are the only organisms that, because of flight ability, have a wide range of distribution. Changes in their composition, diversity and density can indicate changes in the environment. This study was done for the first time with the aims of identification of bird species and their diversity, and ...
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Birds are the only organisms that, because of flight ability, have a wide range of distribution. Changes in their composition, diversity and density can indicate changes in the environment. This study was done for the first time with the aims of identification of bird species and their diversity, and calculation of different ecological indices in a period of five years (2011-16) in 22 sampling sites in Golestan Province, located in Caspian Sea, Iran. From 123 identified bird species belonging to 27 bird families, 6 species have been listed in the International Union for Conservation of Nature. The highest number of birds belongs to the order of Anatidea, but in terms of individual bird species, the Common Coot, Mallard and Greater Flamingo had the greatest numbers. Ecological indices showed that the highest diversity and evenness of species occurred in 2012; during the last year of the study, not only did the number of species decrease, but also, all of the diversity indices declined, while the dominant index significantly increased. The results of this study are a warning for enthusiasts and lovers of environment conservation as well as experts and managers of this field of study, and the need for conservation of these areas is emphasized.
Teymour Tanha
Abstract
Abstract This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal ...
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Abstract This experiment was conducted to study three types of Ruminal degradability of protein whey powder brands with product Shyzr Gloshad Mashhad Food Industries (WP1), whey powder brand Nasr Dalia (WP2) and whey powder product Khorasan Pegah (WP3 ) with 2 Holstein male calves with Ruminal fistula .first, The chemical properties include: moisture, ash, fat, lactose and protein were measured. Later, using Tris buffer samples were extracted proteins and the extracted proteins were determined electrophoresis. For nylon bag technique, the amounts of 5 gram of whey powder samples were placed inside the bag. Incubation times were: 0, 2, 4, 8, 16, 24, 48 and 72 hours. Gel electrophoresis showed that the major protein in whey powder consisted of β- lactoglobulin .lactalbumin, bovine serum albumin and immunoglobulin. These proteins are respectively 50, 20, 10 and 10 percent of total whey proteins in powder form nylon bag Results showed that at 0, 2, 4 and 48 hours incubation, WP1 and WP2 and 72 hours incubation, WP2 highest amount of protein degradability (p < 0.05). At the same time, results also showed that WP1 the highest of soluble protein amount and WP2 highest of protein effective degradability in rumen (p < 0.05). It is also, WP1 and WP2 samples had the highest rate of degradability in 0.20 per hour (p < 0.05). This study showed the highest effective Ruminal degradability of protein whey powder samples related to WP2, WP1, respectively.
Hamed Ostovari; Haji Gholi Kami
Volume 6, Issue 3 , March 2018, , Pages 143-152
Abstract
This research was conducted to preliminary biological study of Eastern Persian toad Bufotes oblongus, in Ghasabe qanats of Gonabad township in Razavi Khorasan province and dam and seasonal spring of Mahvid village and Kowran and Baladeh qanats of Ferdows Township and Mohammad Abad qanats of Qaen township ...
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This research was conducted to preliminary biological study of Eastern Persian toad Bufotes oblongus, in Ghasabe qanats of Gonabad township in Razavi Khorasan province and dam and seasonal spring of Mahvid village and Kowran and Baladeh qanats of Ferdows Township and Mohammad Abad qanats of Qaen township in South Khorasan province at April 2012 to July 2013. Were collected Larvae of these species with the help of hand net (with openings of 1 mm) during the day and collected all adult specimens by hand at the night. The important characteristic of this species is the lump downward of parotoid gland at the rear tympanic membrane and also the small size of tympanic membrane. The maximum length obtained from these species was 8.27 cm (for female) and the minimum length 5.59 cm (for male). In this species the ratio of legs to body size was 0.35 and the ratio of parotoid gland length to body size was 0.21. The results of this research showed in relation to the seasonal spring, there are the threatening factors of these species: polluting water ponds due to the entering of domesticated animal feces into the spawning and larval development place, using that as a drinking water supply and also water evaporation due to the heat. It appears to be a solution for protection of these vertebrate, making plan and carrying out educational projects to raise awareness people is necessary concerning to the amphibians role especially in semi-desert regions of the country.
paria Akbary
Abstract
Abstract
The present study was conducted to evaluate the effect of supplementation of algae (Sargassum ilicifolium) on the growth performances (final weight (FW), condition factor (CF), survival and daily growth ratio (DGR), feed indices (feed conversion rate (FCR), voluntary feed intake (VFI), protein ...
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Abstract
The present study was conducted to evaluate the effect of supplementation of algae (Sargassum ilicifolium) on the growth performances (final weight (FW), condition factor (CF), survival and daily growth ratio (DGR), feed indices (feed conversion rate (FCR), voluntary feed intake (VFI), protein efficiency ratio (PER) and lipid efficiency ratio (LER)) and body composition (protein, fat, moisture and ash) of for Indian mackerel (Rastrelliger kanagurta) 62 days. The experiment was conducted in a completely randomized design with 120 of Indian mackerel (with average weight of 18.93±0.56g and average length of 12.30± 0.17 cm) in 4 treatments and 3 replicates (n=10 in each replicate) and included: control group without usingof supplementation of algae , an another groups (treatment 2, 3 and 4) the amounts of this algae supplement were 5,10 and 15 g/kg food The results showed that at the end of experiment, the highest FW (31.54±1.19g), CF (1.10±0.06%), DGR (23.30±0.38%), the highest PER (27.04±0.53), and the highest LER (115.76±12.84) were observed in the diet containing 15 g /kg supplement and treatment 4 (15 g/kg) and the lowest fat level of body composition (3.10±0.11%) and the highest protein level of body composition (19.46±0.26%) showed a significant difference compared with control treatment (P<0.05). Finally, the present results suggest that diet containing 15 g/kg S. ilicifolium could improve growth, feed performances and carcass quality of R. kanagurta.
Homayoun Khazali; Nazli khajehnasiri; Farzam Sheikhzadeh Hesari
Abstract
Abstract Location of two sets of neurons; pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus, represents a core role of the hypothalamus in the regulation of energy balance. One of the important factors in energy balance is exercise. Until now, the effects ...
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Abstract Location of two sets of neurons; pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus, represents a core role of the hypothalamus in the regulation of energy balance. One of the important factors in energy balance is exercise. Until now, the effects of exercise on hypothalamic POMC and NPY genes expression, weight and appetite control are not clear. Therefore, in the present study, the influence of one-month regular moderate exercise in serum level of corticosterone, as one of the factors affecting appetite, and gene expression of hypothalamic POMC and NPY were discussed.Fourteen adult male Wistar rats were randomly divided into control and one-month regular moderate exercise (RME) groups. After, one-month exercise (22m/min), the arcuate nucleus was isolated from brain and stored in -80 refrigerators for gene expression assay by real-time PCR method. In addition, serum samples were taken to assess corticosterone level by ELISA method. Data were analyzed by Independent t-test in SPSS-16. Pro-opiomelanocortin mRNA level was lower in the group which received exercise less than the control animal (P. The results of the present study indicate that regular moderate exercise is effective in reducing stress but probably increasing anorexigenic neuropeptide, proopiomelanocortin, gene expression is the reason for incapacitation of exercise for losing weight.
Fariba Yaghmori; Reza Hajihosseini; Seyed Mehrdad Kassaee; Bahram Seifizarei
Abstract
Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly ...
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Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly divided into six experimental groups. Rats were treated with indomethacin, celecoxib and aspirin orally with dose of 2 mg/kg b.w for 48h after CLP (Cecal ligation and puncture) injury. Then, blood samples were collected to determine serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and Bilirubin. The extracted livers were used for biochemical assays [Malondialdehyde (MDA), glutathione (GSH) and myeloperoxidase (MPO) activities] and real time PCR studies. The result revealed that treatments significantly improved antioxidant and liver enzymes by reducing MDA, MPO, AST and ALT level and increasing level of GSH. Moreover, cyclooxygenase-2(COX-2) expression in the liver tissue was decreased in the treatment group compared to the CLP group. Thus, our result suggests CLP induced oxidative hepatic damage and NSAIDs have the potential for the treatment of liver damage consecutive to chemical intoxication.
Amir Arasteh; Zolfaghar Lotfi; Mohammad Fazilat; Habibollah Nazem
Volume 6, Issue 2 , December 2017, , Pages 127-137
Abstract
Amyloid fibrils are a class of protein nanoparticles in which native proteins are converted into dense fibers. The aggregation can be pathogenic or non-pathogenic. Recently, these fibrils have been considered for the production of Bio–nanomaterials due to their unique structure. In this study, ...
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Amyloid fibrils are a class of protein nanoparticles in which native proteins are converted into dense fibers. The aggregation can be pathogenic or non-pathogenic. Recently, these fibrils have been considered for the production of Bio–nanomaterials due to their unique structure. In this study, Bovine serum albumin was used as a model protein to optimize the fibrillation process. Concentrations of 2-10 mg/ml were prepared in buffer at different pH values of 3-7 and placed at 0-70 ºC for 0 to 48 hours, and the amounts of produced amyloid fibrils were analyzed by spectrophotometry, fluorimetry and circular dichroism. The spectra obtained from the Congo red absorption method were compared on the basis of the maximum wavelength and absorption at maximum wavelengths with a sample containing Congo red. The concentration of 10 mg/ml of protein, which was 48 hours in buffer at pH = 4 at 50 ºC, produced the highest amounts of amyloid. Optimum conditions were obtained by ThT fluorescence and circular dichroism at a concentration of 10 mg/ml, pH = 4, 70 ºC and 48 hours. The presence of the fibers was confirmed by transmission electron microscope images. The insoluble structure and dimensions of amyloid fibrils can be describe them as new Bio–nanomaterials. Optimizing the production of these structures allows them to be produced at higher scales.
Tohid Vahdatpour; Yahya Ebrahimnezhad; Hosein Cheraghi; Morteza Hesari
Volume 7, Issue 1 , September 2018, , Pages 133-141
Abstract
Abstract The aim of the present study was to investigate the effects of theophylline consumption on the electrocardiography indicators and performance traits of Japanese quails. The experimental diets including control (basal diet), and experimental diets containing of theophylline in levels of: 0.01, ...
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Abstract The aim of the present study was to investigate the effects of theophylline consumption on the electrocardiography indicators and performance traits of Japanese quails. The experimental diets including control (basal diet), and experimental diets containing of theophylline in levels of: 0.01, 0.02, 0.04 and 0.08 percentage of basal diet that supply almost amount 10, 20, 40 and 80 mg/kg of the body weight, respectively. The result showed that the feed conversion ratio in quails fed by theophylline was significant (p
Zahra Moradi; Eisa Solgi
Abstract
The dangers of bio-accumulation of metals at higher levels in the food chain are one of the major concerns in human health. Heavy metals are resistant to degradation and accumulate in the body of aquatic animals, including crayfish. This study was conducted to compare the accumulation of heavy metals ...
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The dangers of bio-accumulation of metals at higher levels in the food chain are one of the major concerns in human health. Heavy metals are resistant to degradation and accumulate in the body of aquatic animals, including crayfish. This study was conducted to compare the accumulation of heavy metals in soft and hard crab blue swimmers of Bushehr shores. Crab samples (40) were collected randomly in the autumn of 1395 using boats and fishing trips from Bushehr coastlines. The acid digestion method was used to prepare the samples. The concentrations of Fe, Zn, Cu, Mn, and Ni in hard tissue were 90.70±43.01, 52.93±6.78, 37.73±13.85, 23.50±7.76 and 4.37±3.66 mg/kg dry weight respectively and in soft tissue were 83.81±56.94, 17.51±4.11, 92.28±28.69, 69.12±3.73 and 4.24±3.90 respectively. Metal concentrations of hard tissue were significantly higher than those in soft tissue. Based on the results in soft crab tissue, the mean concentration of copper was higher than the WHO and NHMRC standards, the mean concentration of nickel was higher than the WHO and FAO standards and the mean concentration of zinc were lower than the global standard. In the hard tissue, the mean value of copper and nickel was higher than the international standards and Zn lower than these standards. Generally, Ni and Cu were higher than the standards that can create risk to human health.
Faraham Ahmadzadeh; Afarin Shahriyari; Hossein Mostafavi
Volume 6, Issue 3 , March 2018, , Pages 153-161
Abstract
Sphaerodactylidae is a family of geckos comprising 200 species in 12 genera. They are distributed in the Northern and Southern America, the Caribbean, Southern Europe, Northern Africa, Middle East, and Central Asia. Individuals of genus Pristurus are also known as the Semaphore Geckos and consist of ...
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Sphaerodactylidae is a family of geckos comprising 200 species in 12 genera. They are distributed in the Northern and Southern America, the Caribbean, Southern Europe, Northern Africa, Middle East, and Central Asia. Individuals of genus Pristurus are also known as the Semaphore Geckos and consist of 23 to 26 species. Pristurus rupestris is one of the smallest species of the genera which is found throughout the Eastern Arabian Peninsula and Southern Iran. In the current study, the origin and distribution of this species in Iran and the islands in which the presence of this gecko was confirmed, as well as the effects of climate oscillation on the distribution of this species during the periods of geologic events with usage of the mitochondrial marker (12S) was studied. In doing so, ten samples were collected from the species distribution range and their sequences were added to sequences obtained from GenBank and the resulted data set was analyzed using Bayesian and Maximum Likelihood methods. The results show that all Iranian samples belong to the Eastern Clade. Also, except for Kharg Island, all samples from other islands of the Persian Gulf are similar to those in the mail land. The Haplotype Network illustrated that both Iranian and Omani samples are placed in the same haplogroup and the central haplogroup is located in Oman. We speculate that during the last Ice Age in Pleistocene era, the Iranian individuals were separated from the Arabian ones and arrived in Iran during the Persian Gulf’s drought. Given that the geomorphologic barriers can directly affect the distribution patterns of reptiles, they would be excellent indicators for studying the phylogeographic
animal biosystematic
rose salatin
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
lepidoptera class consists of butterflies and moths.this class has about 165000 species all over the world which 20000of these species are butterflies.Rhopalocera subclass which belongs to lepidoptera class, is the most familiar and specified of insects.these insects have considerable economical importance ...
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lepidoptera class consists of butterflies and moths.this class has about 165000 species all over the world which 20000of these species are butterflies.Rhopalocera subclass which belongs to lepidoptera class, is the most familiar and specified of insects.these insects have considerable economical importance ,most of these species larvae are vegeterian and a large number of them are crop pests. on the other hand,in the mature stage ,they play an essential role in flowers pollination and growth.a lot of these mature species are gathered for their beauty by collectors and they are used as models in art and drawing. this study has done to complete Elborz butterflies fauna. in researches during spring and summer of 2010 on butterflies fauna in some parts of central Elborz(Dizin, Galandrud, Kamarbon, Nesen, Kodir and Baladeh), 170 butterfly samples of nymphalidae family were gathered by insect net and specified which among them 6 species belong to 3 genus and 2 tribes belong to nymphalinae subfamily.
zahra zamani
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
For environmental safety and reduce the effect of pesticide, for the first report we used the enthomopathogenic fungi Lecanicillium muscarium and Ferula assafoetida extract for Biological control of keys pest that have wide damages to our crops. We isolate the pathogenic strain KB512 of enthomopathogenic ...
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For environmental safety and reduce the effect of pesticide, for the first report we used the enthomopathogenic fungi Lecanicillium muscarium and Ferula assafoetida extract for Biological control of keys pest that have wide damages to our crops. We isolate the pathogenic strain KB512 of enthomopathogenic fungi Lecanicillium muscarium from the soil and recognized.the pathogencity test was carried out with direct insect spray. Bioassay with concentration،1×108 conidi / ml were tested and the control were spraied by 0/01% tween80 in distilled water after spraying the pest, plat were placed in the incubator with temperature of 25±1 and RH80%.Tested pest were monitoring every day for checking the growth of the fungi ,after 9,7,5 days the percentage of mortality in treatment with ( 1×108 conidi/ml) concentration respectively was 36%-25%-17/26% and Ferula assafoetida extract was 40/96%,27/02%,18/62%for Aphis faba, and treatment with ( 1×108 conidi/ml) concentration 40/66%-28/79%-24% % and treatment with Ferula assafoetida extract was 38/9%,35/68%,32/02% for Bemisia tabaci
Mahnaz Shojapour; , Faezeh Fatemi; Reza Haji Hosseini; Marzieh Dehghan Shasaltaneh
Abstract
Abstract Acidithiobacillus ferrooxidans (Af) is an acidophilic bacterium involved in the bioleaching process. Cytochrome c552 (Cyc1) is a periplasmic protein that has a key role in the electron transportation in the respiratory chain. The presence of both heme A and B in the Cyc1 structure and its role ...
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Abstract Acidithiobacillus ferrooxidans (Af) is an acidophilic bacterium involved in the bioleaching process. Cytochrome c552 (Cyc1) is a periplasmic protein that has a key role in the electron transportation in the respiratory chain. The presence of both heme A and B in the Cyc1 structure and its role in taking electrons from the previous protein and electron transfer to the next protein is the main reason for choosing this protein. In this research, with purpose of improving the bioleaching process, glutamate 122 and histidine 54 Cyc1 were selected for point mutation in bioinformatics studies. Mutations were performed by PYMOL software and simulated molecular dynamics for wild proteins, and mutant E122D, H54I in Cyc1. The conformational changes of mutated protein were investigated by SASA, Rg, NH bond analysis. Our results confirmed that the mutated proteins retained its stability during the simulation. By converting glutamate to aspartate, an acid molecule changed into a more acidic molecule leading to the further decreased redox potential at the rusticyanin midpoint resulted into the improved electron transfer from the Rcy to Cyc1 . This will also cause the increased electron transfer rate to heme A. In the case of converting histidine 54 to isoleucine, an electrostatic imidazole loop changed into an amino acid with the root of R with lack of charge which leads to the formation of a stronger hydrogen bond between the two amino acids. Therefore, probably the electron transfers between Cyc1 and CcO is improved through a water molecule (W79) leading to increased bioleaching rate.
Faezeh fatemi; Reza Hajihosseini; Abbas Golbodagh; abolfazl dadkhah
Abstract
Abstract Sepsis is the most common reason of mortality among patients who are in the intensive care unit. Regarding to the side effects of the anti-inflammatory drug consumption, the replace of natural products are suggested in sepsis treatment. the effects of the combination of the deuterium depleted ...
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Abstract Sepsis is the most common reason of mortality among patients who are in the intensive care unit. Regarding to the side effects of the anti-inflammatory drug consumption, the replace of natural products are suggested in sepsis treatment. the effects of the combination of the deuterium depleted water (DDW) and Rosa (R.) damascena Mill. on the stress oxidative parameters and the gene expression of cyclooxygenase-2 (COX-2) in the lung and plasma tissues were investigated. 50 Rats were randomly divided into 5 groups: negative control (LAP), control group (CLP), two treatment groups with the combination of DDW and R. damascena Mill. essential oil (DDW15+EO and DDW30+EO) and positive control group with indomethacin (IND). Then, the levels of oxidative stress parameters and the expression of COX-2 were estimated in plasma and lung tissue. The sepsis resulted in the decrease of ferric reducing antioxidant power (FRAP) and glutathione (GSH) levels along with the increase of lipid peroxidation (LP) andCOX-2 levels. However, the rats treated with the combination of deuterium depleted water and R. damascena Mill. essential oils as same as indomethacin were influenced on the regulation of those parameters through the evaluation of FRAP and GSH levels and the reduction of the LP level and COX-2 gene expression. The pathological studies confirmed the biochemical consequences as well. The results indicated that the oxidative damages were caused by sepsis, but the administration of the natural products such as deuterium depleted water and R. damascena Mill. essential oils could improve the injures due to the effectiveness of oxidative stress and antioxidants parameters.
Safieh Soufian
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Occult hepatitis B infection (OHB) is defined as infection with detectable HBV DNA and undetectable surface antigen (HBsAg) in patients' blood. It is believed that detection of hepatitis B virus by host in “a” region of surface antigen (HBsAg) is performed. However, there have never been successful ...
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Occult hepatitis B infection (OHB) is defined as infection with detectable HBV DNA and undetectable surface antigen (HBsAg) in patients' blood. It is believed that detection of hepatitis B virus by host in “a” region of surface antigen (HBsAg) is performed. However, there have never been successful attempts to provide evidence for this hypothesis, partly because the 3D structure of HBsAg molecule has not been determined. Tertiary structure prediction of HBsAg solely from its primary amino acid sequence may reveal the structural changes of the mutant proteins. We carried out this study to analyze the predicted HBsAg conformation changes of OBH variant and wild isolated from Iranian patient blood. We extracted HBsAg sequences (OBH and wilde) of Iranian from Gen Bank. Tertiary structures of the wild & occult HBsAg were predicted using “ab initio” modeling and I-TASSER method. Then visualized using deep View/Siwss-pdb Viewer. Dipole moments of 3D structures were determined by chimera software. Wild & occult HBsAg sequences were analyzed in CLC Main Workbench to prediction antigenic index of each sequence. Our results showed the single amino acid substitutions in OBH, may alter structural properties of variant HBsAg, which can be shown by both its antigenic index and dipole moment of predicted 3D conformation. This survey suggested that the moreover “a" region mutations, other mutations can be change structure of HBsAg. It suggested to detection and vaccine design should be pay attention to three-dimensional structure of HbsAg.
Fisheries
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
In this study acute toxicity (96h LC50) of EDTA has been carried out in laboratory conditions in 2011 fall for rainbow trout. The experiments were conucted for 96h with standard method (O.E.C.D). LC50 values of EDTA at 96 h were 2231 mgl-1, to the rainbow trout. Then, individuals with a body weight of ...
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In this study acute toxicity (96h LC50) of EDTA has been carried out in laboratory conditions in 2011 fall for rainbow trout. The experiments were conucted for 96h with standard method (O.E.C.D). LC50 values of EDTA at 96 h were 2231 mgl-1, to the rainbow trout. Then, individuals with a body weight of 51 ± 12 g and length 18.5 ± 2.1 cm were selected for six groups (five test group and control group). Onchorhychus mykiss were exposed to 800, 1100, 1400, 1700 and 2100 mgl-1 of EDTA. Fish were exposed for 96 h. The experiments periods were controlled water physicochemical factors such as: pH, total hardness, dissolved oxygen and temperature. For analysis of all data SPSS software program was used. Haematology results show that exposure to EDTA causes an increase in leucocyte count (WBC), notrophil, eosinophil, monocyte and a decrease in haemoglobin (Hb), haematocrit (HCT) and lymphocyte (P0.05). The results showed that exposure to low concentrations of EDTA causes changes in some haematological parameters of rainbow trout and may weaken the fish immune system.
