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Experimental studies on the effect of ‎the scaffold derived decellularized ‎bovine mesentery on the behavior of ‎Blastema cells In vitro

Document Type : Article

Authors

1 Ph. D., Department of Biology, Islamic Azad ‎University, Ayatollah Amoli Branch, Amol, Iran

2 Assistant Professor of Developemental Biology, ‎Ayatollah Amoli Branch Azad University, Amol, Iran

3 Professor, Department of Cell Biology, Kavian, Higher ‎Education Institute, Mashhad, Iran

4 Assistant Professor, Division of Biotechnology, ‎Department of Pathobiology, School of Veterinary ‎Medicine, Ferdowsi University of Mashhad, Mashhad, ‎Iran

Abstract

The interaction between ECM and cells plays an important role in the direction of cellular behavior. So far, different scaffolds have been developed to examine the behavior of cells in three-dimensional conditions. The aim of this study was to investigate the interaction between the mesenteric decellularized scaffold of the bovin and the blastema tissue originated from the pinna of New Zealandwhite rabbit. Mesenteric bovine tissue (5 mm×5 mm) was decellularized using physical methods (slow freezing and snap freeze–thaw) and chemical agents (sodium dodecyl sulfate (SDS) and Triton Х-100) followed by washing and sterilization procedures.These parts were assembled as scaffolds inside the blastema rings from rabbit’s pinna. Sampling was carried out on days 7, 10, 15 and 21. Interactions between the scaffolds and the blastema tissue cells were studied by histological and fluorescence microscopy and electron microscopy.The study of the behavior of Blastema cells in different days of culture in addition to the migration and maintenance of Blastema cells on mesenteric decellularized scaffold showed that these scaffolds were able to restore the structure of blood capillaries, fibroblast and fat cells.Based on histological findings, the results indicate that the Blastema tissue has dynamic cells that can migrate into the scaffold.Furthermore the characteristics of the mesenteric decellularized ECM can support adhesion, migration and differentiation of blastema cells in vitro.

Keywords

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Experimental animal Biology
Volume 8, Issue 4 - Serial Number 4
May 2020
Pages 131-142
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