Document Type : Article
Authors
M.A. in Marine Biology, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
Abstract
Abstract
The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health.
Keywords
References
Abdel-Khalek, A.A.; Kadry, M.A.; Badran, S.R.; Marie, M.A.S. (2015). Comparative toxicity of copper oxide bulk and nano particles in Nile tilapia; Oreochromis niloticus: biochemical and oxidative stress. The Journal of Basic & Applied Zoology; 72: 43-57.
Ahmadi, H.; Naeemi, A.S.; Nazarhaghighi, F.; Ghafuri, H. (2016). Subacute Effects of copper oxide nanoparticles on some hematological indices and gill tissue of the juvenile carp (Cyprinus carpio). Journal of Aquaculture Development; 10(4): 1-14.
Bai, Y.; Yang, T.; Gu, Q.; Cheng, G.; Zheng, R. (2012). Shape control mechanism of cuprous oxide nanoparticles in aqueous colloidal solutions. Powder Technology; 227: 35-42.
Barzegarzadeh-Zarandi, H.; Dabidy-Roshan, V. (2012). Changes in some liver enzymes and blood lipid level following interval and continuous regular aerobic training in old rats. Journal of Shahrekord Uuniversity of Medical Sciences; 14(5): 13-23.
Beyene, H.D.; Werkneh, A.A.; Bezabh, H.K.; Ambaye, T.G. (2017). Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustainable Materials and Technologies; 13: 18-23.
De Smet, H.; Blust, R. (2001). Stress responses and changes in protein metabolism in carp Cyprinus carpio during cadmium exposure. Ecotoxicologyand Environmental Safety; 48(3): 255-262.
Echavarri-Bravo, V.; Paterson, L.; Aspray, T.J.; Porter, J.S.; Winson, M.K.; Hartl, M.G. (2017). Natural marine bacteria as model organisms for the hazard-assessment of consumer products containing silver nanoparticles. Marine Environmental Research; 130: 293-302.
El-Demerdash, F. (2001). Effects of selenium and mercury on the enzymatic activities and lipid peroxidation in brain, liver, and blood of rats. Journal of Environmental Science and Health, Part B; 36: 489-499.
Esmaeel Kaviyani, F.; Naeemi, A.S.; Salehzadeh, A. (2018). Short term effects of zinc oxide nanoparticles on hematological parameters and metabolic enzymes of juvenile Caspian trout (Salmo trutta caspius). Iranian Scientific Fisheries Journal; 26(5): 43-50.
Farah, H.S.; Al-Atoom, A.A.; Shehab, G.M. (2012). Explanation of the decrease in alkaline phosphatase (ALP) activity in hemolysed blood samples from the clinical point of view: In vitro study. Jordan Journal of Biological Sciences; 5(2): 125-128.
Farkas, J.; Christian, P.; Urrea, J.A.G.; Roos, N.; Hassellöv, M.; Tollefsen, K.E.; Thomas, K.V. (2010). Effects of silver and gold nanoparticles on rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicology; 96(1): 44-52.
Fazli, H.; Kor, D.; Daryanabard, G. (2014). Spatial and temporal distribution of Kutum (Rutilus frisii kutum, Kamensky 1901) in Iranian waters of the Caspian Sea. Iranian Scientific Fisheries Journal; 23(1): 63-74.
Ghazinoory, S.; Yazdi, F.S.; Soltani, A.M. (2012). Iran and nanotechnology: A new experience of on time entry. New York, Springer. pp. 85-108.
Hoseini, S.M.; Hedayati, A.; Mirghaed, A.T.; Ghelichpour, M. (2016). Toxic effects of copper sulfate and copper nanoparticles on minerals, enzymes, thyroid hormones and protein fractions of plasma and histopathology in common carp Cyprinus carpio. Experimental and Toxicologic Pathology; 68(9): 493-503.
Hussain, S.; Hess, K.; Gearhart, J.; Geiss, K.; Schlager, J. (2005). In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicology in vitro; 19(7): 975-983.
Imani, A.; Sarvi Moghanlo, K.; Khani, S. (2016). Pathology of copper nanoparticles on liver histoarchitecture and haemato-biochemical parameters of rainbow trout (Oncorhynchus mykiss) fingerlings before and after a recovery period. Veterinary Researches & Biological Products; 29(4): 110-118.
Jahan, S.; Yusoff, I.B.; Alias, Y.B.; Bakar, A.F.B.A. (2017). Reviews of the toxicity behavior of five potential engineered nanomaterials (ENMs) into the aquatic ecosystem. Toxicology reports; 4: 211-220.
Jahanbakhshi, A.; Hedayati, A.; Pirbeigi, A.; Javadimoosavi, M. (2015). Determination of acute toxicity and the effects of sub-acute concentrations of CuO nanoparticles on blood parameters in Rutilus rutilus. Nanomedicine Journal; 2(3): 195-202.
Jiang, H.; Yang, H.; Kong, X.; Wang, S.; Liu, D.; Shi, S. (2012). Response of acid and alkaline phosphatase activities to copper exposure and recovery in freshwater fish Carassius auratus gibelio Var. Life Science Journal; 9(3): 233-245.
Karbassi, A.; Nadjafpour, S. (1996). Flocculation of dissolved Pb, Cu, Zn and Mn during estuarine mixing of river water with the Caspian Sea. Environmental Pollution; 93(3): 257-260.
Mazarei, S.; Sajjadi, M.M.; Sourinejad, I.; Johari, S.A.; Asadi, M. (2015). Effect of lethal concentration of Nano Silver in Aphanius dispar. Journal of Aquatic Ecology; 4(4): 110-115.
Monfared, A.L.; Soltani, S. (2013). Effects of silver nanoparticles administration on the liver of rainbow trout (Oncorhynchus mykiss): histological and biochemical studies.European Journal of Experimental Biology; 3(2): 285-289.
Morganti, P.; Yuanhong, L.; Morganti, G. (2007). Nano-structured products: technology and future. Journal of Applied Cosmetology; 25(4): 159-176.
Oloyede, O.; Sunmonu, T. (2008). Decrease in activities of selected rat liver enzymes following consumption of chemical effluent. Journal of Applied Sciences and Environmental Management; 12(3): 95-100.
Öner, M.; Atli, G.; Canli, M. (2008). Effects of Metal (Ag, Cd, Cr, Cu, Zn) Exposures on Some Enzymatic and Non-Enzymatic Indicators in the Liver of Oreochromis niloticus. Bulletin of Environmental Contamination and Toxicology; 82(3): 317-321.
Perreault, F.; Oukarroum, A.; Melegari, S.P.; Matias, W.G.; Popovic, R. (2012). Polymer coating of copper oxide nanoparticles increases nanoparticles uptake and toxicity in the green alga Chlamydomonas reinhardtii. Chemosphere; 87(11): 1388-1394.
Razmara, P.; Paykan heyrati, F.; Dorafshan, S. (2014). Effect of silver nanoparticles on some hematological parameters of Rainbow catfish, Pangasius hypophthalmus. Journal of Cell & Tissue; 5(3): 263-272.
Sabouri, S.; Falahatkar, B.; Khoshkholgh, M.R.; Poursaeid, S.; Abtahi, B. (2017). Cortisol and Lactate dehydrogenase alternation in juvenile Caspian Kutum (Rutilus frisii) exposed to crude oil pollution. Journal of Animal Research; 30(1): 25-36.
Sadeghi, P.; Attaran Fariman, G.; Kasalkhe, N. (2017). Evaluation the effects of sub-lethal concentrations of zinc chloride on hepatic enzymes activity in grey mullet (Mugil cephalus) in vitro. Journal of Aquatic Ecology; 6(4): 117-123.
Samsami, A.; Rahimi, R.; Shaluei, F.; Hashemi, G. (2017). Effect of colloidal nanosilver particles on some of liver enzymes in common carp (Cyprinus carpio). Journal of Animal Physiology and Development; 10(3): 63-71.
Sarkar, S.; Beitollahi, A. (2009). An overview on nanotechnology activities in Iran. Iranian Journal of Public Health; 38: 65-68.
Shafiei S.S.; Imanpoor, M.R.; Aminian F.B.; Gorgin, S. (2015). Study on morphological structure of Kutum population (Rutilus frisii kutum) in southern of Caspian Sea using Truss network system. Journal of Environmental Science and Technology; 17(3): 115-126.
Shahsavani, D.; Mohri, M.; Kanani, H.G. (2010). Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiology and Biochemistry; 36(1): 39-43.
Soleimani, F.; Nabipour, I.; Faraji C.F.; Dobaradaran, S. (2015). Nanoparticles impact on human and environment: a review of toxicity, exposure, control strategies, and future prospects. Iranian South Medical Journal; 18(3): 630-663.
Song, L.; Vijver, M.G.; Peijnenburg, W.J.; Galloway, T.S.; Tyler, C.R. (2015). A comparative analysis on the in vivo toxicity of copper nanoparticles in three species of freshwater fish. Chemosphere; 139: 181-189.
Taheri, S.; Banaee, M.; Haghi, B.N.; Mohiseni, M. (2017). Effects of dietary supplementation of zinc oxide nanoparticles on some biochemical biomarkers in common carp (Cyprinus carpio). International Journal of Aquatic Biology; 5(5): 286-294.
Vafadarnejad, M.; Gharaei, A.; Mirdar Harijani, J.; Miri, M. (2018). Determining of copper oxide nanoparticles (CuO NPs) LC50 in grass carp (Ctenopharyngodon idella) and its effects on hematological and liver enzymes activity indices. Journal of Fisheries; 71(1): 11-21.
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