Document Type : Article
Authors
1 M.A., Department of Biology, Payame Noor University (PNU), P.O. Box 19395-3697 Tehran, Iran
2 Assistant Professor, Department of Agriculture, Payame Noor University (PNU), P.O. Box 19395-3697 Tehran, Iran
3 Assistant Professor, Department of Biology, Payame Noor University (PNU), P.O. Box 19395-3697 Tehran, Iran
Abstract
Prolongation of wound healing time can be led to chronic infections. Therefor, to achieve infection preventive methods to accelerate of wound improvement process is one of essential needs of Human. In this study, the effects of methanolic extract of Holothuria leucospilota was investigated on restoration of created wound in rat skin. Methanolic extracts of collected sea cucumber from depth of 10 to 15 meters was prepared by rotary. The amount of 0.5 and 1 gr of obtained extraction were added to base ointment. In continuing, 40 rats (average mean of 185±5 gr) were divided into 4 groups which included control, sham (base cream), 0.5% ointment and 1% ointment. Wound (diameter of 6±1 mm) was created on rats’ skin by punch biopsy. Each group were treated (1ml) with prepared concentrations ointment during 13 days. On days of 0, 4, 7, 10 and 13, the wound improvement process was investigated and the diameter of the wounds was measured. According to results, accelerate the wound recovery process was more obvious in treated groups with 0.5% and 1% ointment compared to control and sham groups. Also, there was significant different between treated group with 1%, sham and control during days of 7, 10, 13 (P<0.05).
Keywords
Adibpour, N.; Nasr, F.; Nematpour, F.; Shakouri, A; & Ameri, A. (2014). Antibacterial and antifungal activity of Holothuria leucospilota isolated from Persian Gulf and Oman Sea. Jundishapur Journal of Microbiology; 7: 1-4.
Abraham, T.J.; Nagarajan, J; & Shanmugam, S.A. (2002). Antimicrobial substances of potential biomedical importance from holothurians species. Indian Journal of Geo-Marine Sciences; 31: 161-164.
Afkhami, M.; Ehsanpour, M.; Khazaali, A.; Kamrani, E.; Mokhlesi, A.; & Darvish Bastami, K. (2012). Sea cucumber fisheries of Qeshm Island, Persian Gulf. Beche-de-mer Information Bulletin; 60-61.
Aminin, D.L.; Chaykina, E.L.; Agafonova, I.G.; Avilov, S.A.; Kalinin, V.I.; & Stonik, V.A. (2010). Antitumor activity of the immunomodulatory lead Cumaside. International Immunopharmacology; 10: 648-654. DOI: 10.1016/j.intimp.2010.03.003.
Andersson, L.; Bohlin, L.; Lorizzi M.; Riccio, R.; Minale, L.; & Moreno-López, W. (1989). Biological activity of sea cucumber and saponinlike compounds from starfish and brittle-stars. Toxicon; 27: 179-188. DOI: 10.1016/0041-0101(89)90131-1.
Arfani, A.; Raif, A.; Ginting, C.N.; Ikhtiari, R. (2021). Evaluation of wound healing potential of a sea cucumber (Actinopyga mauritiana) extract in mice (Mus musculus). Jurnal Natural; 21: 168-174. DOI: 10.24815/jn.v21i3.19953.
Aydin, M.; Sevgili, H.; Tufan, B.; Emre, Y.; & Köse, S. (2011). Proximat composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. International Journal of Food Science and Technology; 46: 500-508. DOI: 10.1111/j.1365-2621.2010.02512.x.
Bahroodi, S.; Nematollahi, M.A., Aghasadeghi, M.R., Nazemi, M.; & Behroz, B. (2014). In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota). Iranian Scientific Fisheries Journal; 23 (3):11-19
Barathi, S.S.; Arul, A.; Prathibha, M.D.A.; & Arunkumar, H.D. (2013). Efficacy of gamat extract in wound healing in albino wistar rats. International Journal of Pharmaceutical Sciences Review and Research; 20:142-145.
Beauregard, K.A.; Truong, N.T.; Zhang, H.; Lin, W.; & Beck, G. (2001). The detection and isolation of a novel antimicrobial peptide from the echinoderm, Cucumaria frondosa. Advances in Experimental Medicine and Biology; 484: 55–62. DOI: 10.1007/978-1-4615-1291-2_5.
Bruckner, A.W.; Johnson, K.A.; & Field, J.D. (2003). Conservation strategies for sea cucumbers: Can a CITES Appendix II listing promote sustainable international trade?. Beche-de-mer Information Bulletin; 18: 24- 33.
Castro, P.; & Huber, M.E. (2000). Marine biology.3edition. McGraw-Hill Higher Education, p 444.
Chaphalkar, R.; Apte K. G.; Talekar, Y.; Ojha, S. K.; & Nandave, M. 2017. Antioxidants of Phyllanthus emblica L. Bark extract provide hepatoprotection against ethanol-induced hepatic damage: a comparison with silymarin. Oxidative Medicine and Cellular Longevity. 1-11. DOI: 10.1155/2017/3876040.
Chen, S.; Xue, C.; Yin, L.; Tang, Q.; Yu, G.; & Chai, W. (2011). Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers. Carbohydrate Polymers; 83: 688-696. DOI: 10.1016/j.carbpol.2010.08.040.
Chludil, H.; Muniain, C.; & Maier, M.S. (2002). Cytotoxic and Antifungal Triterpene Glycosides from the Patagonian Sea Cucumber Hemoiedema spectabilis. Journal of Natural Products; 65: 860-5. DOI: 10.1021/np0106236.
Dhinakaran, D.I.; & Lipton, A.P. (2014). compounds from Holothuria atra of Indian ocean. SpringerPlus; 3: 1-10.
Diegelmann, R.F.; & Evans, M.C. (2004). Wound healing: an overview of acute, fibrotic and delayed healing. Frontiers in Bioscience; 9: 283-289. DOI: 10.2741/1184.
Farjami, B.; Nematollahi, M.A.; Moradi, Y.; Irajian, G.R.; Nazemi, M.; Ardebili, A.; & Pournajaf, A. (2013). Antibacterial activity of the sea cucumber Holothuria leucospilota. International Journal of Molecular and Clinical Microbiology; 1: 225-230.
Fredalina, B.D.; Ridzwan, B.H.; Abidin, A.A.; Kaswandi, M.A.; Zaiton, H.; Zali, I.; Kittakoop, P.; & Jais, A.M. (1999). Fatty acid compositions in local sea cucumber. Gen Pharmacol; 33: 337-340. DOI: 10.1016/s0306-3623(98)00253-5.
Ghaderi, R. (2005). Efficacy of epiglue, suture, honey and animal oil in accelerating healing of full thickness wound of skin in mice. Journal of Investigative Dermatology. 17th The annual congress of the European Academy of Dermatology Venereology, 17-21 September 2008, Paris. France.
Ghaderi, R.; Afshar, M.; Akhbarie, H.; & Golalipour, M.J. (2010). Comparison of the Efficacy of Honey and Animal Oil in Accelerating Healing of Full Thickness Wound of mice skin. International Journal of Morphology; 28:193-198.
Gafari, H.; Moradi, Y.; Nazemi, M.; & Safari, R. (2020). Evaluation of anti-inflammatory effects of methanolic extract of Persian Gulf sea cucumber (Holothuria leucospilota) on rats. Journal of Fisheries (Iranian Journal of Natural Resources); 73: 91-100.
Goad, L.J.; Garneau, F.X.; Simard, J.L.; ApSimon, J.W.; and Girard, M. (1985). Isolation of 9(11)-sterols from the sea cucumber. Implications for holothurin biosynthesis. Tetrahedron Letters; 26: 3513-3516.
Herencia, F.; Ubeda, A.; Ferrándiz, M.L.; Terencio, M.C.; Alcaraz, M.J.; García-Carrascosa, M.; Capaccioni, R.; & Payá, M. (1998). Anti-inflammatory activity in mice of extracts from mediterranean marine invertebrates. Life Science 62, 115-120.
Hu, Y.; Chen, J.; Hu, G.; Yu, J.; Zhu, X.; Lin, Y.; Chen, S.; & Yuan, J. (2015). Statistical research on the bioactivity of new marine natural products discovered during the 28 years from 1985 to 2012. Marine Drugs; 13: 202-221.
Ismail, H.; Lemriss, S.; Aoun, Z.B.; Mhadhebi, L.; Dellai, A.; Kacem, Y.; Boirom, P.; & Bouraoui, A. (2008). Antifungal activity of aqueous and methanolic extracts from the Mediterranean sea cucumber, Holothuria polii. Journal of Medical Mycology; 18: 23-26. DOI: 10.1016/j.mycmed.2008.01.002
Kojjoa, A.; & Sawangwong, P. (2004). Drugs and cosmetics from the sea. Marine Drugs; 2: 73-82. 7382. DOI: 10.3390/md202073.
Mazliadiyana, M.; Nazrun, A.S.; & Isa, NM. (2017). Optimum Dose of Sea Cucumber (Stichopus Chloronotus) Extract for Wound Healing. Medical and Health Journal; 12: 83-89. DOI: 10.17576/MH.2017.1201.09.
Mokhlesi, A.; Saeidnia, S.; Gohari, A.R.; Shahverdi, A.R.; Nasrolahi, A.; Farahani, F.; Khoshnood, R.; & E,shaghi, N. (2012). Biological activities of the sea cucumber Holothuria leucospilota. Asian Journal of Animal and Veterinary Advances; 7: 243-249. DOI: 10.3923/ajava.2012.243.249
Mojica, E.R.E.; & Merca, F.E. (2005). Biological properties of lectin from sea cucumber (Holothuria scabra). Journal of Biological Sciences; 5: 472-477. DOI: 10.3923/jbs.2005.472.477.
Mourao, P.A.S.; & Pereira, M.S. (1999). Searching for alternatives to heparin: Sulfated fucans from marine invertebrates. Trends in Cardiovascular Medicine; 9: 225-232. DOI: 10.1016/s1050-1738(00)00032-3.
Oh, G.W.; Ko, S.C.; Lee, D.H.; Heo, S.J.; & Jung, W.K. (2017). Biological activities and biomedical potential of sea cucumber (Stichopus japonicus): a review. Fisheries and Aquatic Sciences; 20: 1-17. DOI: 10.1186/s41240-017-0071-y.
Pacheco, R.G.; Vicente, C.P.; Zancan, P.; & Mourão, P.A.S. (2000). Different antithrombotic mechanisms among glycosaminoglycans revealed with a new fucosylated chondroitin sulfate from an echinoderm. Blood Coagulation and Fibrinolysis; 11: 563-573. DOI: 10.1097/00001721-200009000-00009.
Pangestuti, R.; & Arifin, Z. (2018). Medicinal and health benefit effects of functional sea cucumbers. Journal of Traditional and Complementary Medicine. 8: 341-351. DOI: 10.1016/j.jtcme.2017.06.007.
Pishehvarzad, F.; Yousefzadi, M.; Kamrani, E.; Moini Zanjani, T.; Ali Ahmadi, A.; & keshavarz, M. (2014). Antioxidant activity of extracts of two species of Sea Cucumber Holothuria parva and Holothuria leucospilota from the Persian Gulf, Iran. Journal of Aquatic Ecology; 4 (1): 34-29.
Rafiuddin, A.M.; Venkateshwarlu, U.; & Jayakumar, R. (2004). Multilayered peptide incorporated collagen tubules for peripheral nerve repair. Biomaterials; 25: 85-94. DOI: 10.1016/j.biomaterials.2003.09.075.
Rao, A.B.; Prasad, E.; Deepthi, S.S.; Haritha, V.; Ramakrishna, S.; Madhusudan, K.; & Rao, Y. S.R.V. (2015). Wound healing: a new perspective on glucosylated tetrahydrocurcumin. Drug Design, Development and Therapy; 9: 3579-3588. DOI: 10.2147/DDDT.S85041.
Ridzwan, B.H.; Zarina, M.Z.; Kaswandi, M.A.; Nadirah, M.; & Shamsuddin, A.F. (2001). The antinociceptive effects of extracts from Stichopus chloronotus Brandt. Pakistan Journal Biological Sciences; 4: 244-246. DOI: 10.3923/pjbs.2001.244.246.
Ridswan, B.H.; Leong, T.C.; & Idid, S.Z. (2003). The antinociceptive effects of sea cucumbers Holothuria leucospilota Brandt, Bohadschia marmorata vitiensis Jeager and coelomic Fluid from Stichopus hermanii, Jour. Pakistan Journal Biological Sciences; 6: 2068- 2072.
Roginsky, A.; Singh, B.; Ding, X.Z.; Collin, P.; Woodward, C.; Talamonti, M.S.; Bell, R.H.; & Adrian, T.E. (2004). Frondanol®-A5p from the sea cucumber, Cucumaria frondosa induces cell cycle arrest and apoptosis in pancreatic cancer cells. Pancreas; 29: 335. DOI: 10.1097/00006676-200411000-00048.
Roopesh, J.; & Archana, T. (2007). Sponge: An invertebrate of bioactive potential. Current Science; 93: 444-445.
San Miguel-Ruiz, J.E; and García-Arrarás, J.E. (2007). Common cellular events occur during wound healing and organ regeneration in the sea cucumber Holothuria glaberrima. BMC Developmental Biology; 7: 115. DOI: 10.1186/1471-213X-7-115.
Siahaan, E. A.; Pangestuti, R.; Munandar, H.; & Kim, S.K. (2017). Cosmeceuticals properties of sea cucumbers: prospects and trends. Cosmetics; 4: 1-12. DOI: 10.3390/cosmetics4030026
Tian, F.; Zhang, X.; Tong, Y.; Yi, Y.; Zhang, S.; Li, L.; Sun, P.; Lin, L.; & Ding, J. P. (2005). PE, a new sulfated saponin from sea cucumber, exhibits anti-angiogenic and anti-tumor activities in vitro and in vivo. Cancer biology and therapy; 4: 874-882. DOI: 10.4161/cbt.4.8.1917.
Vieira, R.P.; Mulloy, B.; & Mourão, P.A. (1991). Structure of a fucose-branched chondroitin sulphate from sea cucumber. Evidence for the presence of 3-O-sulfo-D-glucuronosyl residues. Journal of biological chemistry; 21:13530-13536.
Zhang, S.Y.; Khon, A.; & Shahidi, F. (2007). Compositional characteristics and antioxidant properties of fresh and processed seacucumber (Cucumaria frondosa). Agriculture Food Chemistry; 55: 1188-1192. DOI: 10.1021/jf063085h
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