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Evaluation of Plasmid-Mediated ‎Quinolone Resistance Genes and ‎Biofilm Formation in Clinical ‎Isolates of Escherichia coli

Document Type : Article

Authors

1 Instructor, Department of Animal science, School of ‎Agriculture, Payame Noor University, P.O.Box: 19395-3697, ‎Tehran, Iran

2 Assistant Professor, Department of Microbiology, Faculty of ‎Basic Science, Rouzbahan Non-Profit ‎University, Rouzbahan, ‎Iran

Abstract

Escherichiacoli is an opportunistic pathogenic bacterium that causes numerous diseases in humans and animals.Because of increased resistance to antibiotics, this bacterium has raised many concerns in the livestock industry as well as in medicine. The aim of this study was to determine the resistance to fluoroquinolones, to identify plasmid-mediated quinolone resistance (PMQR) genes and to determine the biofilm formation ability of E. coli strains isolated from human and bovine samples in Hamadan.In this descriptive study, 40 isolates of E. coli(20 human isolates, 20 bovine isolates) were studied. First, the resistance of the isolates to ciprofloxacin and levofloxacin was measured by microdilution broth method, and then the identification of fluoroquinolone resistance genes (qnrA, qnrB, qnrC, qnrD, qnrS and acc) was performed by PCR method. In the following, the ability of E. coli isolates to produce biofilm was evaluated by microtiter plate method and the results were analyzed by chi-square test using SPSS software (version 19). Microdilution results showed of 40 E.coli isolates, 26 (65%) were resistant to ciprofloxacin and 23 (57.5%) were found to be resistant to levofloxacin.In PMQR genes frequency analysis by PCR,the qnrA, qnrB, qnrC,qnrD, qnrS and aac genes were detected in 45%, 47.5%, 67.5%, 27.5%, 60% and 55% of the isolates, respectively. Also microtiter plate test results showed that 40% of the isolates were capable of forming strong biofilm and only 4% did not form biofilm.The results of the present study showed that the main mechanism of resistance to fluoroquinolones is related to PMQR genes and possibly excessive use of fluoroquinolones in human infections leads to the development of resistance to these drugs.Biofilm formation has also been shown to be effective in creating fluoroquinolone resistance.

Keywords

References
Akya, A.; Chegenelorestani, R.; Elahi, A.; Hamzavi, Y. (2017). Frequency of Plasmid-mediated Quinolone Resistance Genes in Extended-spectum β-lactamase-producing Escherichia coli. Journal of Mazandaran University of Medical Sciences; 27(151): 41-51.
Alni, R.H.; Ghobadi, N.; Asl, M.N.; Sharifi, A. (2018). Genotyping of Escherichia coli isolated from human and water samples using ERIC-PCR method in Hamadan city. Journal of Birjand University of Medical Sciences; 25(4): 297-306.
Barilli, E.; Vismarra, A.; Frascolla, V.; Rega, M.; Bacci, C. (2008). Escheri chia coli Strains Isolated from Retail Meat Products: Evaluation of Biofilm Formation Ability, Antibiotic Resistance, and Phylogenetic Group Analysis. Journal of Food Protection; 83(2): 233-240.
Bonyadian, M.; Moshtaghi, H., Behroozi P. (2017). Occurrence of verotoxigenic E. coli in cow feces and antimicrobial resistance of the isolates in cattle farms in Shahrekord area. Biological Journal of Microorganism :6(23):75-84.
Boroumand, M.; Sharifi, A.; Manzouri, L.; Khoramrooz, S.S.; Khosravani, S.A. (2019) Evaluation of pap and sfa genes relative frequency P and S fimbriae encoding of uropathogenic Escherichia coli isolated from hospitals and medical laboratories; Yasuj City, Southwest Iran. Iranian Red Crescent Medical Journal; 21(8): 1-8.
Burvenich, C.: Van Merris,V.; Mehrzad, J.; Diez-Fraile, A.; Duchateau, L. (2003). Severity of E. coli mastitis is mainly determined by cow factors. Veterinary research; 34(5): 521-564.
Cattoir, V.; Poirel, L.; Rotimi, V.; Soussy, C-J.; Nordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. Journal of antimicrobial chemotherapy; 60(2): 394-397.
Cavaco, L.; Hasman, H.; Xia, S. (2009). Aarestrup FM. qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovis morbi ficans strains of human origin. Antimicrobial agents and chemo therapy; 53(2): 603-608.
Chen, J.; Griffiths, M. (1998). PCR differen tia tion of Escherichia coli from other Gram‐negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein. Letters in applied microbiology; 27(6): 369-371.
Ciesielczuk, H.; Hornsey, M.; Choi, V.; Woodford, N., Wareham, D. (2013). Develop ment and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance deter minants. Journal of medical micro biology; 62(12): 1823-1827.
Doosti; Mohajer, M.; Pajavand, H.; Abiri R.; Alvandi, A.(2017) Phenotypic and Genotypic Efflux Pumps in Resistance to Fluoroquinolones in E. coli Isolated from Inpatients in Kermanshah Hospitals in 2013. Journal of Arak University of Medical Sciences; 20(9): 22-32.
Ebrahimi, K.A.A.; Shabanpour, Z.; Habibiyan, S., Hakimi-Alni, R.; Hemati, M.; Aflakiyan, F.; et al. (2015). Chlor hexi dine effect on bacterial biofilms iso leated from nosocomial infections. Bio logical Journal Of Micr oorganism; 4(14): 83-92.
EscobarPáramo, P.; Le, Menac’h, A.; Le Gall, T.; Amorin, C.; Gouriou, S.; Picard, B.; et al. (2006). Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Envir onmental microbiology; 8(11): 1975-1984.
FarajzadehSheikh, A.; Veisi, H.; Shahin, M. (2019). Getso M., Farahani A. Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections. Tropical medicine and health; 47(1): 19.
Hakami-Vala, S.A.; Ranjbar, R.; Bagheri Bejetani, A.; Bagheri-Bejestani, F. (2014). Distribution of qnrA gene in fluoroquinolone-resistant Escherichia coli isolates isolated from urine Patients Referred to Imam Khomeini Hospital in Tehran. Journal of Infectious and Tropical Diseases; 64(19): 63-66.
Jalilian, S. (2017). Evaluating the ability of Biofilm formation in Escherichia coli isolated from clinical samples in Zahedan. Journal of Jahrom University of Medical Sciences; 15(1): 36-42.
Jiang, Y.; Zhou, Z.; Qian, Y.; Wei, Z.; Yu, Y.; Hu, S.; et al. (2008). Plasmid-mediated quinolone resistance determinants qnr and aac (6′)-Ib-cr in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in China. Journal of Antimicrobial Chemo therapy; 61(5): 1003-1006.
Junior, J.C.R.; Tamanini, R.; Soares, B.F.; de Oliveira, A.M.; de Godoi Silva, F.; da Silva, F.F.; et al. (2016). Efficiency of boiling and four other methods for genomic DNA extraction of deteriorating spore-forming bacteria from milk. Semina: Ciências Agrárias; 37(5): 3069-3078.
Karah, N.; Poirel, L.; Bengtsson, S.; Sundqvist, M.; Kahlmeter, G.; Nordmann, P.; et al. (2010). Plasmid-mediated quinolone resistance determinants qnr and aac (6′)-Ib-cr in Escherichia coli and Klebsiella spp. from Norway and Sweden. Diagnostic microbiology and infectious disease; 66(4): 425-431.
Kawamura-Sato, K.; Yoshida, R.; Shiba ya ma, K.; Ohta, M. (2010). Virulence genes, quinolone and fluoroquinolone resistance, and phylogenetic back ground of uro patho genic Escherichia coli strains isolated in Japan. Japanese journal of infectious diseases; 63(2): 113-115.
Mah, T-F.C.; O'Toole, G.A. (2001). Mecha nisms of biofilm resistance to anti microbial agents. Trends in micro biology; 9(1): 34-39.
Norouzia; Hossieni, N.H.; Mohebi, S., Kan dehkar, G.M.; Taati, M.M. (2016). Fre quency of plasmid-mediated qnrA, qnrB, and qnrS genes and determination of antibiotic suscep tibility among quinolones and fluoro quinolones resistance Escheri chia coli isolated from Kerman hospitals; 23(148): 98-105.
Rasko, D.A.; Rosovitz, M.; Myers, G.S.; Mon godin, E.F.; Fricke, W.F.; Gajer, P.;et al. (2008). The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of bacteriology; 190(20): 6881-6893.
Rodríguez-Martínez, J.M.; Cano, -M.E.; Velasco, C.; Martínez-Martínez, L.; Pascual, A. (2011). Plasmid-mediated quinolone resistance: an update. Journalof Infection and Chemo therapy; 17(2): 149-182.
Sabaté, M.; Prats, G.; Moreno, E.; Ballesté, E.; Blanch, A.R.; Andreu, A. (2008). Viru lence and antimicrobial resistance pro files among Escherichia coli strains isolated from human and animal wastewater. Research in Microbio logy; 159(4): 288-293.
Sharifi, A.; Mohammadzadeh, A.; Zahraei Salehi, T.; Mahmoodi, P. (2018).Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensisessential oils against Staphylococcus aureus isolates. Journal of applied microbio logy; 124(2): 379-388.
Soleimani-Asl, Y.; Zibaei, M.; Firoozeh, F. (2013). Detection of qnrA gene among quinolone-resistant Escherichia coli isolated from urinary tract infections in Khorram Abad during. Feyz Journal of Kashan University of Medical Sciences; 17(5): 1-7.
Soto, S.; Smithson, A.; Martinez, J.; Hor caja da, J.; Mensa, J.; Vila J. (2007). Biofilm formation in uropathogenic Escheri chia coli strains: relationship with prostatitis, urovirulence factors and antimicrobial resistance. The Journal of urology; 177(1): 365-368.
Tendolkar, P.M.; Baghdayan, A.S.; Gilmore, M.S.; Shanka, N. (2004). Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infection and immunity; 72(10): 6032-6039.
Wareham, D.; Umoren, I.; Khanna, P.; Gordon, N. (2010). Allele-specific polymerase chain reaction (PCR) for rapid detection of the aac (6′)-Ib-cr quinolone resistance gene. Inter national journal of antimicrobial agents; 36(5): 476-487.
Experimental animal Biology
Volume 10, Issue 4 - Serial Number 40
July 2022
Pages 77-86
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