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Vero Cell Culture as a Model for ‎Evaluating the Effects ‎of Macrovipera lebetina Venom

Document Type : Article

Authors

1 Ph.D. Student, Department of Biology, Faculty of science, Payame Noor University, Tehran, Iran

2 Assistant Professor, Department of Biology, Faculty of science, ‎Payame Noor University, Tehran, Iran‎

3 Associate Professor, Biotechnology Research Center, Venom ‎and Biotherapeutics Molecules Laboratory, Pasteur ‎Institute of ‎Iran, Tehran, Iran

4 Assistant Professor, Virology Department, Pasteur Institute of ‎Iran, Tehran, Iran

Abstract

Among Iranian venomous snakes, the most important groups causing envenomation are Naja Naja Oxiana, Echis,Vipera albicornuta, Vipera latifii Mertens, pseudocerastes peersicus and Vipera lebetina. Many researchers believe natural snake venom toxins are containing several pharmacologically active components that could be of potential therapeutic value. In the past, studies have shown that some neurotoxic fractions of snake venom interfere with some infectious and non-infectious diseases such as cancer. we purified snake venom of V. lebetina by fast protein liquid chromatography (FPLC) using Sephacryl S-200 hr column. The fractions collected and evaluated by SDS-PAGE analysis. The cytotoxicity effect of crude venom and fractions on Vero cells were demonstrated using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and adhesion assay. The aim of this study is to investigate the effects of Macrovipera lebetina (one of the native snakes of many parts of iran) , in order to create an appropriate animal cell culture model to evaluate the effects of protein complexes on diseases such as viral infections.

Keywords

References
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Experimental animal Biology
Volume 10, Issue 3 - Serial Number 39
February 2022
Pages 89-98
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