In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Authors

1 Associate Professor, Department of Biology, Faculty of ‎Science, Science and Research Branch, Islamic Azad ‎University, Fars, Iran

2 Assistant Professor, Department of Biology, Faculty of ‎Science, Science and Research Branch, Islamic Azad ‎University, Fars, Iran

3 M.Sc. Student, Department of Biology, Faculty of Science, ‎Science and Research Branch, Islamic Azad University, Fars, ‎Iran

4 Assistant Professor, Department of Biology, Faculty of ‎Science,Islamic Azad University, Izeh Branch, Izeh, Iran

Abstract

Glucocorticoids are observed to increase at anxiety and different stressful situations. This augmentation is driven by stimulation of the hypothalamic-pituitary-adrenal axis which can threaten physical and mental health in long-term. Thus, this study compared the effects of diazepam and currant hydroalcoholic extract on serum corticosterone level in adult male rat. In this reserch, 48 adult male mice were used in 6 groups of 8 animals, including the control (no treatment), Sham (receiving water) and 4 experimental groups receiving doses of 100, 200, 400 mg/kg from currant hydroalcoholic extract and 1 mg/kg of diazepam by gavage. After 15 days, rats perceived stress in elevated plus maze, after which corticosterone level was measured in the serum of the blood collected from their heart. Results were statically analyzed using ANOVA and Tukey's test. The results showed that the doses of 200 and 400 mg/kg from currant extract significantly increases (P≤0.05) and diazepam significantly decreases (P≤0.01) the corticosterone levels, serum. Diazepam reduced corticosterone levels in serum by affecting the benzodiazepine receptors and facilitating inhibitory effect of GABA while currant extract increased it probably by stimulating noradrenergic pathways.

Keywords

Bremmer, JD.; Innis, RB.; Southwick SM, et al. (2000) Decreased benzodiazepine receptor binding in prefrontal cortex in combat-related posttraumatic stress disorder. Am J Psychiatry, 157: 1120-1126.
Brigitte, MK.; Clemens, K.; (2005) Sex differences in HPA axis responses to stress: a review Biological. Psychology. (69): 113-132.
Brunton, LL.; Lazo, JS.; Parker, KL.; (2006) Godman gilmans the pharmacological basis of therapeutics. 11 th ed. Mcgraw hill new York. 429- 460.
Caillet, S.; Salmieri, S.; Lacroix, M.; (2006) Evaluation of free radical-scavening properties of commercial grape phenol extracts by a fast colorimetric method. Food Chem, 95: 1-8.
Campbell, S.; Marriott, M.; Nahmias, C.; MacQueen GM (2004) Lower hippocampal volume in patients suffering from depression: a meta-analysis. Am J Psychiatry, 161(4): 598-607.
Cullinan, WE.; Ziegler, DR.; Herman, JP.; (2008) Functional role of local GABAergic influences on the HPA axis. Brain Struct Funct, 213 (1-2):65-80.
De Kloet, ER.; (2003) Hormones, brain and stress.Endocr Regul, 37: 51-68.
Doymaz, I.; (2006) Drying kinetics of black grapes treated with different solutions. J. Food Engineering, 76: 212-217.
Felt, BT.; Mollen, E.; Diaz, S.; Renaud, E.; Zeglis, M.; Wheatcroft,  G.;  et  al. (2000) Behavioral  interventions reduce infant distress at immunization. Arch Pediatr Adolesc Med, 154 (7): 719-24
Fernandez, E.; Watterberg, K.; (2009) Relative adrenal insufficiency in the preterm and term infant. J of Perinatol, 29 (2): 44-9.
Gerrits, M.; Westenbroek, C.; Fokkema, DS.; Jongsma, ME.; Den Boer, JA.; Ter Horst, GJ.; (2003) Increased stress vulnerability after a prefrontal cortex lesion in female rats. Brain Res Bull, Oct 15; 61 (6):627-35.
Goymann, W.; Wingfield, JC.; (2004) Allostatic load, social status, and stress hormones- the costs of social status matter. Animal Behaviour, 67: 591-2.
Harshal, A.; Pawar Nilesh, M.; (2011) Khutle, Suparna Shukla, Apoorva Ugarkar and Saumya Vijaykumar. Functional Foods And Their Health Benefits: An Overview, International Journal of Advances in Pharmaceutical Research, 2(7): 397-404.
Herman, JP.; Figueiredo, H.; Mueller, NK.; et al. (2003) Central mechanisms of stress integration: hierarchical circuitry controlling hypothalamo-pituitary-adrenocortical responsiveness. Front Neuroendocrinol, Jul; 24 (3):151-80.
Holmes, A.; Parmigiani, PF.; Ferrari, PF.; Palanza, P.; Rodgers, RJ.; (2000) Behavioral profile of wild mice in the elevated plus-maze test for anxiety, Physiology Behavior, 71: 509-16.
Hosseini, SE.; (2013) The effect of interference of Nicotine and immobility stress on performance pituitary–adrenal axis in mature male rats, 3. 20(3):359-366.
Iriti, M.; Faoro, F.; (2006) Grape phytochemicals: A bouquet of old and new nutraceuticals for human health. Med Hypothesis, 67: 833-838.
Jayaprakasha, Gk.; Selvi, T.; Sakariah, KK.; (2003) Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res Int, 36: 117-122.
Levine, A.; Zagoory-Sharon, O.; Feldman, R.; Lewis, JG.; Weller, A.; (2007) Measuring cortisol in human psychobiological studies. Physiol and Behav, 90(1): 43-53.
Mahalaxmi, M.; Swati, S.; Jadhav Veena, S.; Kasture Sanjay, B.; (2009) Kasture Effect of myricetin on behavioral paradigms of anxiety, Pharmaceutical Biology, 47(10): 927-931.
Mantella, RC.; Butters, MA.; Amico, JA.; Mazumdar, S.; Rollman, BL.; Begley, AE.; et al. (2008) Salivary cortisol is associated with diagnosis and severity of late-life generalized anxiety disorder. Psychoneuroendocrinology, 33(6): 773-81.
Marqus de Suuza, L.; Franci, CR.; (2008) GABAergic mediation of stress-induced secretion of corticosterone and oxytocin,but not prolactin, by the hypothalamic paraventricular nucleus. Life Sci, 83(19-20): 686-92.
Mithun, SR.; Sampada, S.; Vineet, M.; Purti, A.; (2011) Herbal Antidepressants. IJPFR, 1(1): 159-169.
Mukesh Yadav.; (2009) Biological and Medicinal Properties of Grapes and Their Bioactive Constituents, An UpdateJ Med Food, 12(3), 473-484.
Nutt, DJ.; (2005) Overview of diagnosis and drug treatment of anxiety disorders. J CNS spectr, 10(1): 49- 56.
Piri, M.; Ayazi, E.; Bananej, M.; Shahin, MS.; (2012) Influence of Dopamine D1 Receptors of the Dorsal Hippocampus on the Histamine-Induced Anxiety Behavior in Mice. ZUMS Journal, 20(79): 1-13.
Putman, P.; Hermans, EJ.; Van Honk, J.; (2010) Cortisol administration  acutely  reduces  threat-selective spatial attention in healthy young men. Physiol & Behav, 99(3): 294-300.
Rabbani, M.;  Sajjadi, SE.;  Khalili, S.; (2011) A Lack of tolerance to the anxiolytic action of Echium amoenum. Res Pharm Sci. Jul-Dec; 6(2): 101-106.
Rezayat, M.; Roohbakhsh, A.; Zarrindast, MR.; Massoudi, R.; Djahanguiri, B.; (2005) Cholecystokinin and GABA interaction in the dorsal hippocampus of rats hn the elevated Plus-maze test of anxiety.Phsyiol Behav, 84(5): 775-782.
Roohbakhsh, A.; Mahmoodi Delfan, K.; Rostami, P.; Hajizadeh Moghaddam, A.; (2008) The Effect of Intracerebroventricular Injection of GABAC Selective Agonist and Antagonist on Anxiety-like Behaviors in Male Rats. Journal of Rafsenjan University of Medical Sciences, 7(1): 13-20.
Schmith, R.; Gazalle, FK.; Lima, MS.; Cunha, A.; et al. (2005) The efficacy of antidepressanta for G.A.D. Asystematic review and meta- analysis. J rev bross psiqiatr, 27(1): 18-24.
Tollenaar,  MS.;  Jansen,  J.;  Beijers,  R.;  Riksen-Walraven, JM.; Weerth, Cd.; (2010) Cortisol in the first year of life:  Normative  values  and  intra-individual variability. Early Hum Dev. 86(1): 13-6.
Torres, IL.; Vasconcellos, AP.; Silveira Cucco, SN.; Dalmaz, C.; (2001) Effect of repeated stress on novelty-induced antinociception in rats. Braz J Med Biol Res, 34: 241-244.
Tsigos, C.; Chrousos, GP.; (2002) Hypothalamic-pituitary-adrenal axis, neuroendocrine factors and stress. J.Psychosom. Res, 53, 865-871.
Xu, Y.; Li, S.; Chen, R.; Li, G.; Barish, PA.; You, W.; Chen, L.; Lin, M.; Ku, B.; Pan, J.; Ogle, WO.; (2010) Antidepressant-like effect of low molecular proanthocyanidin in mice: involvement of monoaminergic system, Pharmacol Biochem Behav, 94: 447-53.
Yu, G.; Chen, H.; Xingium, WU.; Shannon, G.; Matta Burt, M.; (2010) Nicotine self-administratration modulates glutamate and GABA transmission in hypothalamic paraventricular nucleus to enhance in hypothalamic-pituitary-adrenal response to stress. J Neurochem, 113(4): 919-29.