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Molecular and biochemical ‎investigation of the role of ‎streptococcus iniae in mortality of ‎Lates calcarifer cages culturing in ‎the Persian Gulf

Document Type : Article

Authors

1 Ph.D. of Aquatic Animal Health, Faculty of Veterinary, Shahid ‎Chamran University of Ahvaz, Iran

2 Professor, Department of clinical Sciences, Faculty of ‎Veterinary, Shahid Chamran University of Ahvaz, Iran

3 Professor, Department of pathobiology, Faculty of Veterinary, ‎Shahid Chamran University of Ahvaz, Iran

4 Assistant Professor, Agricultural Education and Extension ‎Research Organization, Fisheries Science Research Institute, ‎South IRAN Aquaculture Research Center,Ahvaz, Iran

Abstract

Streptococcus iniae is a gram-positive bacterium that causes invasive infections with severe septicemia and meningitis as well as high economic losses in freshwater and sea fish. It is one of the most infectious diseases in Asian sea bass. The purpose of the present study was to investigate the role of this bacterium in marine bass fish mortality in farmed cages on the northern coast of the Persian Gulf.  In this study, a number of 150 cultured Asian sea bass (60 fish suspected of bacterial infection and 90 apparently healthy fish) were selected and bacterial sampling were taken from head kidneys and brain of fish from sea bass cages of Bushehr, Khuzestan and Hormozgan provinces. After isolation and purification of the bacteria, the presence of Streptococcus iniae was evaluated and confirmed via biochemical tests and molecular methods (with specific primers of lctO gene). The PCR results and sequencing of the PCR product indicate high prevalence of Streptococcus iniae in Asian sea bass cages culturing in the Persian Gulf. Therefore, effective measures should be taken to prevent Streptococcosis (especially vaccination).

Keywords

References
Akbary, P.; Mirvaghefi, A.R.; Akhlaghi, M.; Fereidouni, M.S. (2015). Influence of Maternal and Larval Immunisation against Lactococcus garviae Infection in Rainbow Trout Oncorhynchus mykiss (Walaum) Lysozyme Activity and IgM Level. Open Journal of Animal Sciences; 3: 258-265.
Akhlaghi, M.; Keshavarzi, M. (2002). The occurrence of streptococcosis in the cultured rainbow trout of Fars province, Iranian Journal of Veterinary Research; 3: 183-189.
Akhlaghi, M.; Mahjor, A.A. (2004). Some histopathological aspects of streptococcosis in cultured rainbow trout (Oncorhynchus mykiss). Bulletin European Association of Fish; 24(3): 132-136.
Al Harbi, A.H. (2011). Molecular characterization of Streptococcus iniae isolated from hybrid tilapia [Oreochromis niloticus x Oreochromis aureus]. Aquaculture; 312: 15-18.
Allen, J.P.; Neely, M.N. (2011). The Streptococcus iniae transcriptional regulator CpsY is required for protection from neutrophil-mediated killing and proper growth in vitro. Infection and Immunity; 79: 4638-48.
Altinok, I. (2011). Multiplex PCR assay for detection of four major bacterial pathogens causing rainbow trout disease. Dis Aquat Org.;93(3):199-206.
Austin, B.; Robertson, P.A. (1993). Recovery of Streptococcus milleri from ulcerated koi carp (Cyprinus carpio L.) in the UK. Bulletin- European Association of Fish Pathologists; 13: 207-209.
Bercovier, H.; Ghittino, C.; Eldar, A. (1997). Immunization with bacterial antigens: infections with streptococci and related organisms. Developments in Biological Standardization; 90: 153-160.
Bromage, E.S. (1997). Streptococcus iniae, a bacterial infection in barramundi. Lates calcarifer Bloch. Diseases of Aquatic Organisms dis Aquat Org.; 36: 177-181
Brousseau, R.; Hill, J.E.; Préfontaine, G.; Goh, S.H.; Harel, J.; Hemmingsen, S.M. (2001). Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences. Appl. Environ. Microbiol.; 67(10), 4828-4833.
Buller, N. (2014). Bacteria and fungi from fish and other aquatic animals: a practical identification manual, Department of Agriculture and Food Western Australia. (2nd edi.). Includes bibliographical references and index. ISBN 978-1-84593-805-5
Candice, M.; Justice, C.F.; Baiano, C.C.; Benedict, Y.; Fabian, A. (2012). Evolution of the Capsular Operon of Streptococcus iniae in Response to Vaccination. Applied and Environmental Microbiology, 78: 8219-8226.
Foo, J.T.; Ho, B.; Lam, T.J. (1985). Mass mortality in Siganus canaliculatus due to streptococcal infection. Aquaculture; 49: 185-196.
Holt, J.G.; Krieg, R.N.; Sneath, P.H.A.; Staley, J.T.;  Williams, S.T.  (1993).    Bergey’s  Manual of Determinative Bacteriology. (9th ed.). Williams and Wilkins, Baltimore, PP: 221-251.
Kayansamruaj, P.; Dong, H.T.; Nguyen, V.V.; Le, H.D.; Pirarat, N.; Rodkhum, C. (2017). Susceptibility of freshwater rearing Asian seabass (Lates calcarifer) to pathogenic Streptococcus iniae. Aquaculture Research; 48(2): 711-718.‏
Klesius, P.H.; Shoemaker, C.A.; Evans, J.J. (1999). Efficacy of a killed Streptococcus iniae vaccine in tilapia (Oreochromis niloticus). Bulletin- European Association of Fish Pathologists; 19: 39-41.
Locke, J.B.; Aziz, R.K.; Vicknair, M.R.; Nizet, V.; Buchanan, J.T. (2008). Streptococcus iniae M-ike protein contributes to virulence in fish and is a target for live attenuated vaccine development. PloS One; 3: 2824-2829.
Locke, J.B.; Colvin, K.M.; Varki, N.; Vicknair, M.R.; Nizet, V.; Buchanan, J.T. (2007). Streptococcus iniae beta-hemolysin streptolysin S is a virulence factor in fish infection. Diseases of Aquatic Organisms; 76: 17-26.
Lowe, B.A.; Miller, J.D.; Neely, M.N. (2007). Analysis of the polysaccharide capsule of the systemic pathogen Streptococcus iniae and its implications in virulence. Infection and Immunity; 75: 1255-1264.
Lu, M. (2010). Review of research on streptococcosis in tilapia. South China Fisheries Science; 6: 75-79.
Mathew, G. (2009). Taxonomy, identification and biology of seabass (Lates calcarifer).
Mirzakhani A .(2009). Isolation of Streptococcusiniae and Lactococcus garvieae in rainbow trout farms in Chahramahal va Bakhtiari Province by Multiplex PCR [Dissertation]. Shahrekord: Islamic Azad Univ.
Molloy, S. (2009). Bacterial immune evasion: an evasive surface. Nature Reviews Microbiology; 7: 762-772.
Nakanishi, T.; Kiryu, I.; Ototake, M. (2002). Vaccine development of a new vaccine delivery method for fish: percutaneous administration by immersion with application of a multiple puncture instrument. Vaccine; 20: 3764-9.
Nayak, S.K. (2010). Role of gastrointestinal microbiota in fish. Aquaculture Research; 41: 1553-1573.
Nguyen, T.L.; Lim, Y.J.; Kim, D.H.; Austin, B. (2016). Development of real‐time PCR for detection and quantitation of Streptococcus parauberis. Journal of Fish Diseases; 39(1), 31-39.
Ooyama, T.; Kera, A.; Okada, T.; Inglis, V.; Yoshida, T. (1999). The protective immune response of yellowtail Seriola quinqueradiata to the bacterial fish pathogen Lactococcus garvieae. Diseases of Aquatic Organisms; 37: 121-126.
Paterson, B.D.; Rimmer, M.A.; Meikle, G.M.; Semmens, G.L. (2003). Physiological responses of the Asian sea bass, Lates calcarifer to water quality deterioration during simulated live transport: acidosis, red-cell swelling, and levels of ions and ammonia in the plasma. Aquaculture, 218(1-4), 717-728.
Robins, J.B.; Halliday, I.A.; Staunton-Smith, J.; Mayer, D.G.; Sellin, M.J. (2005). Freshwater-flow requirements of estuarine fisheries in tropical Australia: a review of the state of knowledge and application of a suggested approach. Marine and Freshwater Research; 56(3), 343-360.
Singh, R.K. (2000). Growth, survival and production of Lates calcarifer in a seasonal rain fed coastal pond of the Konkan region. Aquaculture; 8: 55-60.
Soltani,  M.; Tarahomi,  M. (2008). Study of streptococcosis/lactococcosis in some farmed rainbow trout in Fars province, Iran. The first International Congress on Aquatic Animal Health Management and Diseases, Tehran, Iran. p.124.
Soltani, M.; Alishahi, M.; Mirzargar, S.; Nikbakht, G. (2007). Vaccination of rainbow trout against Streptococcusiniae infection: comparison of different routes of administration and different vaccines. Iranian Journal of Fisheries Sciences; 7: 129-140.
Soltani, M.; Jamshidi, S.; Sharifpour, I. (2005). Streptococcosis caused by Streptococcus iniae in farmed rainbow trout (Oncorhynchys mykiss) in Iran: biophysical characteristics and pathogenesis. Bulletin of the European Association of Fish; 25(3): 95-106.
Suanyuk, N.; Sukkasame, N.; Tanmark, N.; Yoshida, T.; Itami, T.; Thune, R.L.; ...; Supamattaya, K. (2010). Streptococcus iniae infection in cultured Asian sea bass (Lates calcarifer) and red tilapia (Oreochromis sp.) in southern Thailand. Songklanakarin Journal of Science & Technology, 32(4).‏
  Wanman, C.H.; Tanmark, N.; Supamattaya, K. (2007). Production of killed vaccine from Streptococcus sp. and its application in sea bass (Lates calcarifer). Songklanakarin Journal of Science and Technology; 29: 1251-61.
Whitehead, P.J.; Wilson, B.A.; Bowman, D.M.J.S. (1990). Conservation of coastal wetlands of the Northern Territory of Australia: the Mary River floodplain. Biological Conservation; 52(2): 85-111.
Wozniake, M.  (1996). The Role of carotenoids in fish. Protectio Aquarum Et Piscatoria; 22: 65-75.
Experimental animal Biology
Volume 9, Issue 3 - Serial Number 35
January 2021
Pages 19-27
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