Comparison of the Antifungal ‎effects of various extracts of aerial ‎part and root of Echium italicum ‎on Candida species with two ‎antibiotics Nystatin and ‎Fluconazole

Nabipour, Fatemeh; Fazilati, Mohammad; Dousti, Behroze; Mir Derikvand, Reza

doi:10.30473/eab.2019.45696.1700

Document Type : Article

Authors

¹ Ph. D., Department of Biology, Faculty of Basic ‎Sciences, Payame Noor University, Isfahan, Iran

² Professor, Department of Biology, Faculty of Basic ‎Sciences, Payame Noor Univercity, Isfahan, Iran

³ Assistant Professor, Department of Biology, Faculty of ‎Basic Sciences, Islamic Azad University, ‎Khorramabad Branch, Lorestan, Iran

⁴ Assistant Professor, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Khorramabad Branch, ‎Lorestan, Iran

https://doi.org/10.30473/eab.2019.45696.1700

Abstract

Recently, due to increased Candida infection and frequent recurrence and due to the resistance of Candida species to most antifungal drugs, for the first time, antifungal properties of methanolic, n-hexane and aqueous extracts from different parts of Echium italicum were compared with antibiotics. In this study, methanol, n-hexane and aqueous extracts from different parts of the plant were prepared in different concentrations by soaking method And on two species of Candida glabrata and Candida tropicalis by disk diffusion method and MIC (minimum inhibitory concentration), MFC (minimum fungicidal concentration of growth) by micro dilution method. Antibiotics Nystatin and Fluconazole were used as a positive control and DMSO was used as a negative control. Data were analyzed by one-way ANOVA and t-test. The results showed that different extracts from the root of the plant showed more antifungal activity than the aerial part of the plant against both species of Candida (P<0/05). The methanolic extract of the root showed the highest mean diameter of growth and the lowest MIC (15.62 μg/μl) and MFC (31.25 μg/μl) against Candida glabrata as inhibitory diameter of growth the methanolic extract in concentration of 5mg/ml (18.5±0.5) was higher than Nystatin antibiotic inhibitory diameter of growth for Candida glabrata. The results showed that the extracts of this plant have antifungal effects on both Candida species and after supplementary and clinical trials as an appropriate antifungal agent against Candida glabrata infection and Tropicalis infection.

Keywords

20.1001.1.23222387.1399.9.1.11.4

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Experimental animal Biology

Comparison of the Antifungal ‎effects of various extracts of aerial ‎part and root of Echium italicum ‎on Candida species with two ‎antibiotics Nystatin and ‎Fluconazole

References

References

Volume 9, Issue 1 - Serial Number 1
September 2020
Pages 123-133

Comparison of the Antifungal ‎effects of various extracts of aerial ‎part and root of Echium italicum ‎on Candida species with two ‎antibiotics Nystatin and ‎Fluconazole

References

References

Volume 9, Issue 1 - Serial Number 1September 2020Pages 123-133

Volume 9, Issue 1 - Serial Number 1
September 2020
Pages 123-133