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Analgesic effect of Satureja ‎bachtiarica Bunge essential oil ‎using formalin test in mice

Document Type : Article

Authors

1 M.A., Department of Biology, Izeh Branch, Islamic ‎Azad University, Izeh, Iran

2 Associate Professor,Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran

3 Assistant Professor, Department of Biology, Sanandaj ‎Branch, Islamic Azad University, Sanandaj, Iran

Abstract

The application of herbal plants instead of synthetic drugs is increasing in recent years because of their lower side-effects and high varieties of efficient components. The aim of this study was to investigate the analgesic effect of Satureja bachtiarica Bunge essential oil by formalin test in mice model in this study, S. bachtiarica Bunge essential oil was prepared using clevenger apparatus by distilled water method. Mature male rats (25-35 gr) were selected. Acute and chronic pain induction was performed by injection of 20μl of 4% formalin solution to evaluate the analgesic effects of S. bachtiarica essential oil. The study groups consisted of the control group receiving normal saline, the groups receiving 25, 50 and 100 mg/ kg of S. bachtiarica essential oil, and the positive control group received morphine 10 mg/ kg. Single dose injections were performed 30 minutes before the start of the test. Acute pain (5 minutes after injection) and chronic pain (20 minutes after inoculation) were measured by stopwatch and the results were analyzed by SPSS16 software.S. bachtiarica Bunge essential oil at doses of 25, 50 and 100 mg/kg significantly moderated formalin-induced pain as it could reduce foot licking caused by formalin injection in both acute and chronic phases. The present data indicate that essential oil of S. bachtiarica Bunge has analgesic effect on mice, and this plant could be considered in future health care.

Keywords

References
Aeschbach, R.; Löliger, J.; Scott, B.; Murcia, A.; Butler, J.; Halliwell, B.; et al. (1994). Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food and Chem Toxicol; 32(1): 31-6.
Apkarian, A.V.; Hashmi, J.A.; Baliki, M.N. (2011) Pain and the brain: specificity and plasticity of the brain in clinical chronic pain. Pain; 152(3 Suppl): 49-52.
Barnes, P.J.; Belvisi, M.G.; Rogers, D.F. (1990) Modulation of neurogenic inflammation: novel approaches to inflammatory disease. Trends Pharmacol Sci; 11(5): 185-189
Glass, C.K.; Saijo, K.; Winner, B.; Marchetto, M.C.; Gage, F.H. (2010) Mechanisms underlying inflammation in neurodegeneration. CelL; 140(6):918-34.
Hajhashemi, V.; Ghannadi, A.; Pezeshkian, S.K. (2002) Antinociceptive and anti-inflammatory effects of Satureja hortensis L. extracts and essential oil. J Ethnopharmacol; 82(2-3): 83-87
Hashemi, M.B.; Niakousari, M.; Saharkhiz, M.J. (2011) Antioxidant activity of Satureja bachtiarica Bunge essential oil in rapeseed oil irradiated with UV rays. Eur J Lipid Sci Technol; 113(9): 1132-7.
Looi, Y.; Audisio, R. (2007) A review of the literature on post-operative pain in older cancer patients. Eur J Cancer; 43(15): 2222-30.
Memarzadeh, S.M.; Pirbalouti, A.G.; AdibNejad, M. (2015) Chemical composition and yield of essential oils from Bakhtiari savory (Satureja bachtiarica Bunge.) under different extraction methods. Ind Crops Prod; 76: 809-16.
Nazıroğlu, M.; Dikici, D.M.; Dursun, Ş. (2012) Role of oxidative stress and Ca2+ signaling on molecular pathways of neuropathic pain in diabetes: focus on TRP channels. Neurochem Res; 37(10):2065-75.
Parada, C.; Tambeli, C.; Cunha, F.; Ferreira, S. (2001) The major role of peripheral release of histamine and 5-hydroxytryptamine in formalin-induced nociception. Neurosci; 102(4):937-44.
Rabiei, Z.; Gholami, M.; Rafieian-Kopaei, M. (2016) Antidepressant effects of Mentha pulegium in mice. Bangladesh J Pharmacol; 11(3):711-5
Riella, K.R.; Marinho, R.R.; Santos, J.S.; Pereira-Filho, R.N.; Cardoso, J.C.; Albuquerque-Junior, R.L.C.; Thomazzi, S.M. (2012) Anti-inflammatory and cicatrizing activities of thymol, a monoterpene of the essential oil from Lippia gracilis, in rodents. J Ethnopharmacol; 143: 656-663
Saghaei, F., Motamedi, S. (2017) The antinociceptive and anti-inflammatory effects of hydro alcoholic extract of Satureja bachtiarica on mouse. J Herbal Drugs; 8(3): 179-184
Sefidkon, F.; Jamzad, Z. (2000) Essential oil of Satureja bachtiarica Bunge. J Essential Oil Res; 12(5):545-6.
Sefidkon, F.; Sadeghzadeh, L.; Teymouri, M.; Asgari, F.; Ahmadi, S. (2007) Antimicrobial effects of the essential oils of two Satureja species (S. Khuzistanica Jamzad and S. bachtiarica Bunge) in two harvesting time.  Iranian J Med Aroma Plants; 23(2): 174-182.
Shabrandi, S.h.; Yousofvand, N.; Zarei, F. (2016) Effect of Dietary Virgin Olive (Olea europaea) Oil on Nociception and Its Effect on Morphine-induced Analgesia in Male Mice Using Formalin Test. Iranian J Nutr Sci Food Technol; 11: 1; 43-50
Telleria-Diaz, A.; Schmidt, M.; Kreusch, S.; Neubert, A.K.; Schache, F.; Vazquez, E.; et al. (2010) Spinal antinociceptive effects of cyclooxygenase inhibition during inflammation: involvement of prostaglandins and endocannabinoids. Pain; 148(1): 26-35.
Verdi, J.; Sabet Kasaei, M.; Kamalinejad, M.; Sharif, Sh. (2005) Antinociceptive effects of water extracts of Satureja hortensis seedlings in male rats. Physiol Pharmacol; 2(8): 163-168
Youdim, K.A.; Deans, S.G. (2000) Effect of thyme oil and thymol dietary supplementation on the antioxidant status and fatty acid composition of the ageing rat brain. British J Nutr; 83(01): 87-93.
Experimental animal Biology
Volume 9, Issue 1 - Serial Number 1
September 2020
Pages 103-110
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