In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Document Type : Article

Authors

1 M. A. of Food Hygiene and Quality Control, ‎Department of Food Hygiene and Aquaculture, ‎Faculty of Veterinary Medicine, Ferdowsi University ‎of Mashhad, Mashhad, Iran

2 Associate Professor, Department of Food Hygiene and ‎Aquaculture, Faculty of Veterinary Medicine, ‎Ferdowsi University of Mashhad, Mashhad, Iran

3 Professor, Department of Food Hygiene and ‎Aquaculture, Faculty of Veterinary Medicine, ‎Ferdowsi University of Mashhad, Mashhad, Iran

4 Associate Professor, Department of Basic Sciences, ‎Faculty of Veterinary Medicine, Ferdowsi University ‎of Mashhad, Mashhad, Iran

Abstract

Oxidation is one of the most harmful chemical processes that affect the properties of food and health of consumers. The aim of this study was to determine the process of changes in the biomarkers of meat oxidation in trout during storage at 4°C as well as comparing these treatments with similar ones on the market. For this purpose the samples of trout (n=15) collected and maintained at 4°C for 96 hours and their MDA and carbonyl groups were measured every 24 hours (0, 24, 48, 72 and 96 hours). Also, the same samples (n=30) were taken from the markets to measure those parameters. MDA levels in trout increased significantly from the initial value of 0.117 μmol/gw (in time of catch) to 0.189 μmol/gw within 96 hours (P<0.05). The amount of carbonyl groups in the salmon increased significantly from initial value of 1463.72 nmol/gw to 1756.19 nmol/gw within the 96 hours. MDA level of market trout was 0.17032 μmol/gw and did not show a significant difference with the samples collected and maintained for 72 hours. The amount of carbonyl groups in the trout of the market was 1551.15 nmol/gw and there was no significant difference with the treated samples during 48 and 72 hours. A Significant increase in the level of malondialdehyde and carbonyl groups of meat indicate an increase in the oxidation of lipids and proteins during storage at 4°C.

Keywords

Afonso, C.; Cardoso, C.; Lourenço, H.M.; Anacleto, P.; Bandarra, N.M.; Carvalho, M.L.; Castro, M.; Nunes, M.L. (2013). Evaluation of hazards and benefits associated with the consumption of six fish species from the Portuguese coast. Journal of food composition and analysis, 32(1): 59-67.
Alasalvar, C.; Miyashita, K.; Shahidi, F.; Wanasundara, U. (2011). Handbook of seafood quality, safety and health applications. John Wiley & Sons.
Augustyniak, E.; Adam, A.; Wojdyla, K.; Rogowska-Wrzesinska, A.; Willetts, R.; Korkmaz, A.; Atalay, M.; Weber, D.; Grune, T.; Borsa, C.; Gradinaru, D. (2015). Validation of protein carbonyl measurement: a multi-centre study. Redox biology, 4: 149-157.
Baron, C.P.; Kjaersgard, I.V.; Jessen, F.; Jacobsen, C. (2007). Protein and lipid oxidation during frozen storage of rainbow trout (Oncorhynchus mykiss). Journal of agricultural and food chemistry; 55(20): 8118-8125.
Celi, P. (2010). The role of oxidative stress in small ruminants' health and production. Revista Brasileira de Zootecnia; 39: 348-363.
Davies, M. (2012). Free radicals, oxidants and protein damage. Australian Biochemist; 43: 8-12.
Del Rio, D.; Stewart, A.J.; Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, metabolism and cardiovascular diseases; 15(4): 316-328.
Eymard, S.; Baron, C.P.; Jacobsen, C. (2009). Oxidation of lipid and protein in horse mackerel (Trachurus trachurus) mince and washed minces during processing and storage. Food Chemistry; 114(1): 57-65.
Goli, Z.; Lakzaee, M.; Pouramir, M. (2010). Antioxidant activity of sour orange peel extract and its effect on lipid oxidation in raw and cooked fish Hypophthalmichthys molitrix. Iranian Journal of Nutrition Sciences & Food Technology; 5(2): 19-26.
He, Y.; Huang, H.; Li, L.; Yang, X.; Hao, S.; Chen, S.; Deng, J. (2018). The effects of modified atmosphere packaging and enzyme inhibitors on protein oxidation of tilapia muscle during iced storage. LWT-Food Science and Technology; 87: 186-193.
Jiang, W.D.; Feng, L.; Liu, Y.; Jiang, J.; Hu, K.; Li, S.H.; Zhou, X.Q. (2010). Lipid peroxidation, protein oxidant and antioxidant status of muscle, intestine and hepatopancreas for juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of myo-inositol. Food chemistry; 120(3): 692-697.
Latha, M.; Pari, L. (2003). Preventive effects of Cassia auriculata L. flowers on brain lipid peroxidation in rats treated with streptozotocin. Molecular and cellular biochemistry; 243(1): 23-28.
Lykkesfeldt, J. (2001). Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry. Clinical Chemistry; 47(9): 1725-1727.
Lykkesfeldt, J. (2007). Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinica chimica acta; 380(1): 50-58.
Lyu, F.; Shen, K.; Ding, Y.; Ma, X. (2016). Effect of pretreatment with carbon monoxide and ozone on the quality of vacuum packaged beef meats. Meat science; 117: 137-146.
Nakyinsige, K.; Sazili, A.Q.; Aghwan, Z.A.; Zulkifli, I.; Goh, Y.M.; Bakar, F.A.; Sarah, S.A. (2015). Development of microbial spoilage and lipid and protein oxidation in rabbit meat. Meat science; 108: 125-131.
Ogino, K.; Wang, D.H. (2007). Biomarkers of oxidative/nitrosative stress: an approach to disease prevention. Acta Medica Okayama; 61(4): 181-189.
Okolie, N.P.; Okugbo, T.O. (2013). A comparative study of malondialdehyde contents of some meat and fish samples processed by different methods. Journal of Pharmaceutical and Scientific Innovation; 2: 26-29.
Okpala, C.O.R.; Choo, W.S.; Dykes, G.A. (2014). Quality and shelf life assessment of Pacific white shrimp (Litopenaeus vannamei) freshly harvested and stored on ice. LWT-Food Science and Technology; 55(1): 110-116.
Popova, T.; Marinova, P.; Vasileva, V.; Gorinov, Y.; Lidji, K. (2009). Oxidative changes in lipids and proteins in beef during storage. Archiva Zootechnica; 12(3): 30-38.
Prego, R.; Pazos, M.; Medina, I.; Aubourg, S.P. (2012). Comparative chemical composition of different muscle zones in angler (Lophius piscatorius). Journal of food composition and analysis; 28(2): 81-87.
Richards, M.P.; Hultin, H.O. (2002). Contributions of blood and blood components to lipid oxidation in fish muscle. Journal of Agricultural and Food Chemistry; 50(3): 555-564.
Soyer, A.; Ozalp, B.; Dalmış, U.; Bilgin, V. (2010). Effects of freezing temperature and duration of frozen storage on lipid and protein oxidation in chicken meat. Food chemistry; 120(4): 1025-1030.
Zheng, J.; Huang, T.; Yu, Y.; Hu, X.; Yang, B.; Li, D. (2012). Fish consumption and CHD mortality: an updated meta-analysis of seventeen cohort studies. Public health nutrition; 15(4): 725-737.