Pathogenicity assessment of an indigenous strain of Trichoderma harzianum Rifai (Ascomycota: Hypocreales) to the grasshopper, Chrotogonus trachypterus (Orth.: Pyrgomorphidae) (B.) in laboratory conditions

Hamzei, Mostafa; Mirshekar, Ali; Salari, Mohammad; Khani, Abbas; Sabbagh, Seyed Kazem

doi:10.30473/eab.2018.5182

Document Type : Article

Authors

¹ Lecturer, Payame-Noor University of Zabol, Iran.

² Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Iran.

³ Associate Professor, Department of Biology, Campus of Science, Yazd University, Iran

https://doi.org/10.30473/eab.2018.5182

Abstract

Abstract
The Sugarcane grasshopper (Chrotogonus trachypterus) is one of the most important and indigenous and harmful pest of field crops in Sistan region of Iran and cause considerable damage especially during seedling stages. We conducted this study in order to introduction of a biocontrol agent to C. trachypterus in a completely randomized designs and factorial experiment with three replications using topical application under laboratory conditions. For inoculations, after the preliminary experiments, five fungal suspensions prepared at consecrations of 1×10⁴, 1×10⁵, 1×10⁶, 1×10⁷ and 1×10⁸ conidia mL^-1 in distilled water containing 0.05% Tween-80^®. Grasshoppers typically inoculated under the pronotal shield using a Hamilton microaplicator. Control treatment were treated with distilled water containing 0.05% Tween-80^® as surfactant. The corrected cumulative percent mortality was recorded daily until 15 days after inoculations. Percentage of insect mortality was analyzed using (log-probit) methods.Results showed T. harzianum was pathogenic to adult insects and insect mortality increased with increasing concentrations. The highest and lowest mortality observed at 10⁸ conidia mL^-1 and 10⁴ conidia mL^-1 with a 75 and 25 percent respectively (p₅₀value was 1.01×10⁶conidia mL^-1. This is the first report of the pathogenicity of T. harzianum to the grasshopper, C. trachypterus.

Keywords

20.1001.1.23222387.1397.7.2.7.8

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Experimental animal Biology

Pathogenicity assessment of an indigenous strain of Trichoderma harzianum Rifai (Ascomycota: Hypocreales) to the grasshopper, Chrotogonus trachypterus (Orth.: Pyrgomorphidae) (B.) in laboratory conditions

References

References

Volume 7, Issue 2 - Serial Number 26
December 2018
Pages 71-80

Pathogenicity assessment of an indigenous strain of Trichoderma harzianum Rifai (Ascomycota: Hypocreales) to the grasshopper, Chrotogonus trachypterus (Orth.: Pyrgomorphidae) (B.) in laboratory conditions

References

References

Volume 7, Issue 2 - Serial Number 26December 2018Pages 71-80

Volume 7, Issue 2 - Serial Number 26
December 2018
Pages 71-80