Document Type : Article
Authors
1 Former M. Sc. Student, Department of Biology, Faculty of Science, Islamic Azad University, Lahijan Branch, Lahijan, Iran
2 Assistant Professor, Department of Marine Biology, Faculty of Science, Islamic Azad University, Lahijan Branch, Lahijan, Iran
3 Assistant Professor, Department of Aquaculture, MirzaKoochak Khan Higher Education Center for Fishery Science and Technology, Rasht, Iran
4 Former M. Sc. Student, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
Abstract
Heat shock proteins belong to a big family of proteins which are heat-tension dependent and are inductable by heat-stress. They can save the living organisms against suddenly fluctuation of environmental conditions and cause the stability of other protein. Pontogammarus maeoticus is one of the most abundant amphipods at the south of Caspian Sea coast. In this study the presence of heat shock proteins in Pontogammarus (male and female species) by treating at laboratory conditions and levels of temperature (20, 25 and 30 oC) was investigated. Samplings were carried out at coast of Hassan Rood (guilan province). The levels of proteins were determined by Bradford and ELISA methods. Statical analyses of data were carried out by one-way ANOVA and Duncan test. The results showed that by temperature increasing, the levels of Heat- shock proteins and absorbtion of ELISA were increased and the highest absorbance was observed in treating at 30 oC after four hours. Increasing the treating temperature between 5 until 10 oC more than the optimal value causes the more synthesis of heat shock protein 70 with molecular weights of 66.5 and 90 KD both in male and female species of Pontogammarus. In other words the results of this study express that by increasing the temperature the level of heat shock protein 70 will be increasing.
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