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The survey of comparison the effects of selenium nanoparticles and selenit sodium on hematological factors of sheep

Document Type : Article

Authors

1 Assistant Professor, Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Associate Professor, Department of Clinical Science, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

3 Professor, Department of Clinical Science, School of Veterinary Medicine, Shahrekord University

Abstract

The aim of the present study was to compare the effects of oral administration of nanoparticles-selenium and selenit sodium on peripheral blood factors in sheep. For the purpose fifteen sheep, were randomly divided to three groups and to groups 1 nanoparticles-selenium (1mg/kg), prepared by selenium dioxide reduction using ascorbic acid, to groups 2 selenit sodium (1mg/kg) and to group 3 as control group distilled water were orally given for 10 consecutive days. Then, the blood sample were collected from the jugular vein of all sheep into the tubes containing heparin on days 0,10,20 and 30 and on them were determined the hematological factors including hematocrit, erythrocytes, hemoglobin, white blood cell, platelet and total protein concentration was determined by autoanalyzer. For differential cell counting, after the preparation of candle flame smear, were fixed by methanol and were stained with giemsa for 20 minutes. Then the cells number of neutrophils, eosinophils, basophils, monocytes and lymphocytes differential count were by lens 100 of optical microscopy and cedar oil. Statistical analysis in the hematocrit, erythrocytes, hemoglobin, platelets, fibrinogen and total protein showed no significant difference among the three groups on different days. But the survey of the white blood cells count showed a significant increase in group1 on day30 with day 0 and means comparison in group-1 with control group on day 30. Also, the neutrophils count determined a significant increase ingroup 1 on day 30 with day 10 and means comparison in group-1 with two and control groups on day10. The survey of the lymphocytes count showed a significant decrease inmeans comparison in group-1 with two and control groups on day10. The study revealed nanoparticles-selenium compared with sodium selenite increase, the number of white blood cells and neutrophils with greater intensity.
 

Keywords

References
Abbas, AK.; Lichtman, A.H.; Pillai, S.; (2012). Cellular and Molecular Immunology. (7th ed). Elsevier. Philadelphia, USA. p. 55-88.
Arvillomi, H.; Poikonen, K.; Jokinen, I.; (1983). Selenium and Immune function in humans. Infection Immunology; 41: 185-189.
Bickhardt, K.; Ganterm, M.; Sallmann, P.; (1999). Investigation of the manifestation of vitamin E and selenium deficiency in sheep and goats. Dtsch Tierarztl Wochenschr; 106: 242-247.
Fraga, C.G.; Ariass, R.F.; Llesuy, S.F.; (1987). Effect of Vitamin E and Se deficiency on rat liver chemiluminescence. Biochemical Journal; 242: 383-392.
Hodgson, J.C.; Watkins, C.A.; Bayne, C.W.; (2006). Contribution of respiratory burst activity to innate immune function and the effect of disease status and agent on chemiluminescence responses by ruminant phagocytes in vitro. Veterinary Immunology Immunopathology; 112: 12-23.
Huang, B.; Zhang, J.; Hou, J.; Chen, C.; (2003). Free radical scavenging efficiency of ano-Se in vitro. Free Radical Biology and Medicine; 35: 805-813.
Jain, N.C.; (1986). Schalm’s Veterinary Hematology. (4th ed). Lea & Febiger Publication. Philadelphia, USA. p. 381-383.
Kojouri, G.A.; Sadeghian, S.; Mohebbi, A.; Mokhber Dezfouli, M.R.; (2012). The effects of oral consumption of selenium nanoparticles on chemotactic and respiratory burst activities of neutrophils in comparison with sodium selenite in sheep. Biological Trace Elemental Research; 146: 160-6.
Kojouri, G.A.; Sharifi, S.; (2013). Preventing Effects of Nano-Selenium Particles on Serum Concentration of Blood Urea Nitrogen, Creatinine, and Total Protein during Intense Exercise in Donkey. Journal of Equine Veterinary Science; 33: 597-600.
Lessard, M.; Yang, W.C.; Elliott, G.S.; (1991). Cellular immune response in Pigs fed a vitamin E & Se Deficient Diet. Journal of Animal Science; 69: 1575-1582.
Li, H.; Zhang, J.; Wang, T.; Luo, W.; Zhou, Q.; Jiang, J.; (2008). Elemental selenium particles at nano-size (Nano-Se) are more toxic to Medaka (Oryzias latipes) as a consequence of hyper-accumulation of selenium: a comparison with sodium selenite. Aquatic Toxicology; 89: 251-256.
Lumsden, J.H.; Valli, V.E.O.; McSherry, B.J.; (1974). The Piromen test as an assay of bone marrow Granolocyte reserves in the calf. I. Studies on bone marrow and peripheral blood leukocytes. The Canadian Journal of Comparative Medicine; 38: 56-64.
Nandra, R.K.; (1997). Nutrition and the immune system: an introduction. The American Jornal of Clinical Nutrition; 66: S460-S463.
Pighetti, G.M.; Eskew, M.L.; Reddy, C.C.; Sordillo, L.M.; (1998). Selenium and vitamin E deficiency impair transferrin receptor internalization but not IL-2, IL-2 receptor, or transferrin receptor expression. Journal of Leukocyte Biology; 63: 131-137.
Radostits, O.M.; Gay, C.C.; Hinchcliff, K.W.; Constable, P.D.; (2007). Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs and goats, (10th ed). Elsevier. Spain. p.552-557.
Roitt, I.P.; Martin, S.; Burton, D.; (2006). Roitt,s Essential Immunology, (11th ed). Wiley-Blackwell. England. p. 115-119.
Sadeghian, S.; Kojouri, GA.; Mohebi, A.; (2012). Nanoparticles of Selenium as species with stronger physiological effects in sheep in comparison with sodium selenite. Biological Trace Elemental Research; 146: 302-8.
Smith, B.P.; (2015). Large Animal Internal Medicine, (5th ed). Elsevier. Missouri. USA. p. 1062-1064.
Tizard, I.R.; (2013). Veterinary immunology. (9th ed). Elsevier. China. p. 30-40.
Van Vleet, J.F.; (1982). Comparative efficacy of five supplementation procedures to control selenium-vitamin E deficiency in swine. American Journal of Veterinary  Research; 43: 1180-1186.
Jacobs, R.M.; Smith, H.E.; Whetstone, C.A.; Suarez, D.L.; Jefferson, B.; Valli, V.E.O.; (1994). Haematological and lymphocyte subset analyses in sheep inoculated with bovine immunodeficiency-like virus.  Veterinary  Research Communication; 18: 471-482.
Wang, H.; Zhang, J.; Yu, H.; (2007). Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radical Biology and Medicine; 42: 1524-1533.
Xin, Z.; Waterman, D.F.; Hemken, R.W.; (1991). Effect of copper status on neutrophil Function in steers. Journal of Dairy Science; 74: 3078-3085.
Zhang, J.; Wang, H.; Bao, Y.; Zhang, L.; (2004). Nano red elemental selenium has no size effect in the induction of seleno-enzymes in both cultured cells and mice. Life Science; 75: 237-244.
Zhang, J.S.; Wang, H.L.; Yan, X.X.; Zhang, L.D.; (2005). Comparison of short-term toxicity between nano-Se and selenite in mice. Life Science; 76: 1099-1109.
Zhang, J.S.; Wang, X.F.; Xu, T.W.; (2008). Elemental selenium at nano size (Nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with Se-methylselenocysteine in mice. Toxicology Science; 101: 22-31.
 
Experimental animal Biology
Volume 6, Issue 1 - Serial Number 1
No.1
May 2017
Pages 11-22
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