Kinetic investigations of peroxidase in roots of  Gundelia Tournefortii

Saeidian, Shahriar

Document Type : Article

Author

Shahriar Saeidian

Assistant Professor of Biochemistry, Department of Biology, Payame Noor University, Tehran, Iran

Abstract

Kinetic properties of root of peroxidase of Gundelia tournefortii investigated at different pH, temperature and different inhibitors. Maximum activity of peroxidase achieved at pH 5.5-6 in according to type of substrate. Activity of peroxidase increased at constant concentration of H₂O₂ and different concentration of guaiacol, catechol and pyrogallol. The maximum activity in presence of 22mM of guaiacol was earned 2.4 Unit/mg.protein. At constant concentration of guaiacol and different concentration of H₂O₂, V_max and K_m were 2 Unit/mg.protein and 4 mM respectively. Activity decreased at high concentration of H₂O₂ so reached to 35% of V_max at 40 mM. Catalytic efficiency of peroxidase in presence of constant concentrations of guaiacol and H₂O₂ calculated 0.5 and 0.5 Unit/mg protein mM^-1 respectively. Stability of enzyme and maximum of activity was earned at 40 ⁰C. Sodium cyanid and sodium azide showed inhibitory effect on activity with IC50 of 0.008 and 0.4 mM respectively. These results of inhibitors showed that inhibitory effect of Azid is 312 times of inhibitory effect of cyanide. Cyanid is competitive inhibitor of peroxidase in presence of catechol and pyrogallol and noncompetitive inhibitor of enzyme in presence of guaiacol, and Azide is noncompetitive inhibitor of peroxidase of root in gundelia tournefortii in presence of catechol and pyrogallol and uncompetitive inhibitor of enzyme in presence of guaiacol and kojic acid is uncompetitive inhibitor of peroxidase in presence of guaiacol and pyrogallol.

Keywords

20.1001.1.23222387.1395.5.2.1.8

References

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Experimental animal Biology

Kinetic investigations of peroxidase in roots of Gundelia Tournefortii

References

References

Volume 5, Issue 2 - Serial Number 2
December 2016
Pages 1-11

Kinetic investigations of peroxidase in roots of Gundelia Tournefortii

References

References

Volume 5, Issue 2 - Serial Number 2December 2016Pages 1-11

Volume 5, Issue 2 - Serial Number 2
December 2016
Pages 1-11