In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Document Type : Article

Authors

Abstract

Abstract
This study was carried out to investigate the hepatoprotective effect of deuterium depleted water with Satureja rechingeri essential oil on liver damage. For this purpose, the adult male rats were divided into 16 groups. The negative control group used tap water in 14 days following DMSO i.p injection at day 15th. The treatment group with acetaminophen received tap water in 14 days following 500mg/kg b.w. i.p injection of acetaminophen dissolved in 300ml DMSO at day 15th. The treatment groups receiving deuterium depleted water (30ppm and 60 ppm) plus i.p injection of Satureja rechingeri essential oil following 500 mg/kg b.w. acetaminophen injection at day 15th.Then, at 4,8,16 and 24 hours after acetaminophen injection, the blood was taken from hurt puncture and the liver tissues were removed. Then, the biochemical marker levels such as AST, ALT, ALP and bilirubin were measured in plasma. The glutathione s-transferase enzyme activity and protein level, cytochrome P450 protein level and glutathione level were also estimated in liver homogenate. Findings indicated no significant changes in the level of serum parameters such as ALT, ALP and bilirubin in groups. While injecting acetaminophen decreased the levels of GST and GSH and increased significantly the level of CYP450 and AST. Treatment with deuterium depleted water and Satureja rechingeri essential oils are effective in moderating the levels of these parameters, leaded to returning of these parameters to normal values. Finally, the treatment of rats with high dose of acetaminophen caused liver oxidative damage and using of deuterium depleted water and Satureja rechingeri essential oil can be effective in preventing and improving these damages.
 

Keywords

Adams, ML.; Pierce, RH.; Vail, ME.; White, CC.; Tonge, RP.; Kavanagh, TJ.; Fausto, N.; Nelson, SD.; Bruschi, SA; (2001). Enhanced Acetaminophen Hepatotoxicity in Transgenic Mice Overexpressing BCL-2. Molecular Pharmacology; 60(5): 907-915.##Adewusi, EA.; Afolayan, AJ.; (2010). A review of natural products with hepatoprotective activity. J Med Plant Res; 4(13): 1318-1334.##Allameh, A.; Nickseresht, M.; Kheyrdoosh, F.; (1999). Role of cytosolic glutation S-transferase   in protemtion aginst acetaminophen-induced lipid peroxidation in weanling rats. Med J; 133: 213-217.##Allameh, A.; Nickseresht, M.; Kheyrdoosh, F.; (1999). Role of cytosolic glutation S-transferase in protemtion aginst acetaminophen-induced lipid peroxidation in weanling rats. Med J.; 133: 213-217.##Bradford, MM.; (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem; 72: 248-254.##Canatese, J.; Gimson, AES.; Davis, M.; Williams, R.; (1981). Factors conlribuling mortality in paracetamol induced hepatic failure. B Med J; 282: 199-201.##Catherine, A.; Evans, R.; Nicholas, JM.; Geoge, P.; (1996). structure antioxidante activity relationships of flavoniods and phenolic acids. free radic biol med; 20: 933-56.##Chattopadahyay, RR.; (2003). possible mechanism of hepatoprotective activity of azadirachta indica leaf extract: part ii. J ethnopharmacol; 62: 31-39.##Chiu, S.; Bhakthan, NMG.; (1978). Experimental acetaminophen-induced hepatic necrosis. Lab. Invest; 39: 193.##Chun, LJ.; Tong, MJ.; Busuttil, RW.; Hiatt, JR.; (2009). Acetaminophen Hepatotoxicity and Acute Liver Failure, J Clin Gastroenterol; 43(4), 342-349.##Council on Scientific Affairs; (2010). Science Brief on Acetaminophen and Liver Injury. May.##Dadkhah, A.; Allameh, A.; Khalafi, H.; Ashrafi-Helan, J.; (2001a). Inhibitory effects of dietary caraway essential oils on 1,2-shrafi-Helan J. Inhibitory effects of dietary caraway essential oils on 1,2-dimethylhydrazine-induced colon carcinogenesis is mediated by liver xenobiotic metabolizing enzymes. Nutrition and Cancer; 63: 43-54##Dadkhah, A.; Fatemi, F.; Ababzadeh, S.; Roshanaei, K.; Alipour, M.; Sadegh Tabrizi, B.; (2014). Potential preventive role of Iranian Achillea wilhelmsii C. Koch essential oils in acetaminopheninduced hepatotoxicity, Botanical Studies; 55: 37.##Dixon, M.; Dixon, B.; Aparicio, SR.; Loney, D.; (1975). experimental paracetamol-induced hepatic necrosis: a light and electron-microscopy and histochemical study. J. Pathol; 116: 17-29.##Doina, P.; Olariu, V.; Scurtu, M.; Tulcan, C.; Brudiu, I.; Muntean, D.; Petcu, F.; Padeanu, I.; Ostan, M.; (2012). The effect of deuterum depleted water on some hepatic enzymes’activity in rats intoxicated with chromium (vi), ecotoxicologie, Zootehnie Si Tehnologii De Industrie Alimentara.##Doina, P.M.; Victor Olariu, V.; Scurtu, M.; Tulcan, C.; Ileana Brudiu, I.; Muntean, D.; Petcu, F.; Pădeanu, I.; Ostan, M.; (2012). The effect of deuterum depleted water on some hepatic enzymes’ activity in rats intoxicated with chromium (vi), fascicula: ecotoxicologie, zootehnie si tehnologii deindustrie alimentară; 521-526.##Dong, H.; Haining, RL.; Thummel, KE.; Rettie, AE.; Nelson, SD.; (2000). Involvement of Human Cytochrome P450 2D6 in the Bioactivation of Acetaminophen. Drug Metabolism and Disposition; 28(12): 1397-1400.##El-Banna, H.; Soliman, M.; Al-wabel, N.; (2013). Hepatoprotective Effects of Thymus and Salvia Essential oils on Paracetamol-Induced Toxicity in Rats, J. Phys. Pharm. Adv.; 3(2): 41-47.##Fatemi, F.; Allameh, A.; Khalafi, H.; Ashrafihelan, J.; (2010a). Hepatoprotective effects of g-irradiated caraway essential oils in experimental sepsis. Appl Radiat Isotopes; 68: 280-285.##Fatemi, F.; Allameh, A.; Khalafi, H.; Rajaee, R.; Rezaei, MB.; (2011). Biochemical properties of γ-irradiated caraway essential oils. J Food Bio chem; 35: 650-62.##Fatemi, F.; Allameh, A.; Khalafi, H.; Rezaei, MB.; Seyhoon, M.; (2010b). The effect of essential oils and hydroalcoholic extract of caraway seed on oxidative stress parameters in rats suffering from acute lung inflammation before and after γ-irradiation. J Med Aroma Plant; 25(4): 441-455.##Garba, S.H.; Sambo, N.; Bala, U.; (2009). The effect of the aqueous extract of kohautia grandiflora on paracetamol induced liver damage in albino rats. Nigerian Journal of Physiological Sciences; 24(1): 17-23.##Giulivi, C.; Romero, FJ.; Cadenas, E.; (1992). The iteraction of trolox,a water-soluble vitamin E analog, with reduction of the oxoferyl moity. Arch. Biochem. Biophys; 299(1): 302-312.##Goodall, K.; (2003). The Role of Deuterium in DNA degradation, Anti-Aging Medical News, Resource.##Guo Ying, Zuo.; Fan Yan, Meng.; Xiano Yan, Hao.; Yan Ling, Zhang.; Gen Chun, Wang.; Gui Li, Xu.; (2008). Antibacterial alkaloids from Chelidonium majus L. against clinical isolates of methicillin resistant Staphylococcus aureus. J. Pharm. Pharmacent. Sci.; 11(4): 90-94.##Hayes, JD.; Pulford, DJ.; (1995). The glutathione-S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol; 30: 445-600.##Heidarian, E.; Jafari-Dehkordi, E.; Seidkhani-Nahal, A.; (2011). Effect of garlic on liver phosphatidate phosphohydrolase and plasma lipid levels in hyperlipidemic rats. Food Chem Toxicol; 49(5): 1110-1114.##Hinson, J.A.; Phol, L.R.; Monks, T.J.; Gillette, J.R.; (1981). Acetaminophen   induced hepatotoxicity. Life Science; 29: 107-111.##James, LP.; Mayeux, PR.; Hinson, JA.; (2003a). Acetaminophen-induced hepatotoxicity. Drug Metabolism and Disposition; 31(12): 1499-1506.##James, L.P.; Mayeux, P.R.; Hinson, J.A.; (2003). Acetaminophen-induced hepatotoxicity. Drug Metab Dispos; 31(12): 1499-1506.##Jose, J.K.; Kuttan, R.; (2000). Hepatoprotective activity of Emblica officinalis and Chyavanaprash. Journal of Ethnopharmacology; 72: 135-140.##Kanchana, N.; Mohamed sadiq, A.; (2011). Hepatoprotective effect of plumbago zeylanica on paracetamol induced liver toxicity in rats. Int J Pharm Pharm Sci.; 3(1): 151-154.##Khorsandi, LS.; Taheri mobarakeh, M.; Kalantari, H.; (2007). The protective effects of Curcuma longa extract on acetaminophen-induced acute hepatotoxicity in mice. J Rafsanjan Univ Med Sci.; 6(4): 219-226. (Persian)##Knight, TR.; Fariss, MW.; Farhood, A.; Jaeschke, H.; (2003). Role of Lipid Peroxidation as a Mechanism of Liver Injury after Acetaminophen Overdose in Mice, Toxicological Sciences; 76: 229-236.##Kumar, S.; Gautam, S.; Powar, S.; Sharma, A.; (2010). Microbial decontamination of medicinally important herbals using gamma radiation and their biochemical characterisation. Food Chem.; 119: 328-335.##Kumari, A.; Kakkar, P.; (2012). Lupeol prevents acetaminophen-induced in vivo hepatotoxicity by altering the Bax/Bcl-2 and oxidative stress-mediated mitochondrial signaling cascade. Life Sci.; 90(15-16): 561-570.##Larson, AM.; Polson, J.; Fontana, RJ.; Davern, TJ.; Lalani, E.; Hynan, LS.; et al., (2005). Acetaminophen-induced acute liver failure: results of a United States multicenter, prospective study, Hepatology; 42: 1364-72.##Mann, J.M.; Louis, M.P.; Kragel, P.J.; Kragel, A.H.; Robert, W.C.; (1989). Cardiac consequences of massive acetaminophen overdose. The Amencan Journal of Cardiology; 63: 1018-1020.##Miric, R.E.; (2010). Deuterium-depleted water in cancer therapy, Environmental Engineering and Management Journal; 9(11): 1543-1545.##Mitchell, Jr.; Jollow, D.J.; Potter, W.Z.; (1973a). Acetaminophen-induced hepatic necrosis i. role of drug metabolism. J. Parmacol-Exper. Terap.; 187: 185 194.##Mudge, G.H.; Gembory, M.W.; Duggin, G.G.; (1978). Covalent binding of metabolites of acetaminophen to kidney protein and depletion of renal glutathione. J Pharmcol Exp Ther.; 206(1): 218-226.##Nithianantham, K.; Shyamala, M.; Chen, Y.; Latha, LY.; Jothy, SL.; Sasidharan, S.; (2011). Hepatoprotective potential of Clitoria ternatea leaf extract against paracetamol induced damage in mice. Molecules; 6(12): 10134-45.##Olariu, L.; Petcu, M.; Tulcan, C.; Chis-Buiga, I.; Pup, M.; Florin, M.; Brudiu, Ileana.; (2007). Deuterium depleted water- antioxidant or prooxidant?, lucrari stiinłifice medicina veterinara vol. xl, timisoara.##Olariu, L.; Petcu, M.; Tulcan, C.; Chis-Buiga, I.; Pup, M.; Florin, M.; Brudiu, Ileana.; (2007). Deuterium depleted water- antioxidant or prooxidant? lucrari stiinłifice medicina veterinara vol. xl, timisoara.##Rowden, AK.; Norvell, J.; Eldridge, DL.; Kirk, MA.; (2005). Updates on acetaminophen toxicity. Medical Clinics of North America; 89(6): 1145-1159.##Sabina, E.; Rasool, M.; Vedi, M.; Navaneethan, D.; Ravichander, M.; Parthasarthy, P.; Rachel Thella, S.; (2013). Hepatoprotective and antioxidant potential of withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci.; 5(2): 648-651##Sefidkon, F.; Abbasi, K.; Jamzad, Z.; Ahmadi, S.; (2007). The effect of distillation methods and stage of plant growth on the essential oil content and composition of Satureja rechingeri Jamzad. Food Chemistry, 100(3): 1054-1058.##Seldak, J.; Limdsay.; (1986). Estimation of total protein bound and non-protein sulfidryl groups in tissue with Elman,s reagent. Anal. Biochem.; 25: 192-205.
Swan, DK.; Ford, B.; (1997). Chemoprevention of cancer: review of the literature. Oncol Nurs Forum; 24: 719-727.##Walker, RM.; Racz, WJ.; McElligott, TF.; (1983). Scanning electron microscopic examination of acetaminophen-indced hepatotoxicity and congestion in mice. Am. J. Pathol; 113: 321-330.##Weil, K.; Hooper, L.; Afzal, Z.; Esposito, M.; Worthington, HV.; van Wijk, AJ.; Coulthard, P.; (2007). Paracetamol for pain relief after surgical removal of lower wisdom teeth, Cochrane Database Syst Rev.; 18(3): CD004487.##